Rmu_sc0001311.1_g000026

C1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001311.1
OG0000109
Physical Location & Seq
Forward (+)
113100 .. 114587
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001311.1_g000026.1.cds

Sequence Viewer

Length: 1002 bp
atgtacatgatggtggaggaggtctcagctcgtatggctgttgcgcttgtaatcaaggacaatggaagagtgtcgtttggggttgctggcttatctgtgatctccaatcatgcctatgcaattgggaggcttctgtcacagaagaactttgattcacgtcttttcatggctatcatggctaatgaatgggataagatcttattcggaggaccgtggttttatggtcgttccgtgcttgctttggtggagtataacggctgctgtgaggcaaagtcggtgtctatttcagcgatctcgatctgggtggaagtgattagtctccctcctatactaatgatcattgaagctgtctccatgattggtgcttctttggtgccgacagatatgtcaggaacggaggtcatgactggggcacagcatgttgccgagcatttggaggtgggtggttctaaccctaggtctaacccgcctcgtgttttttattttgttgggtcgggttcgacatcggggctgatgcctcaggctgctacaagaccgcttacatgtagtagcatgtcggctggtatcgttccatcgccttctggtctggttccttctgcatctggcaactccatagaaaagatatcaatacaagcaattcctccctgtcctcgatcaagtggtgctcccggtgatctcctctccatgccggccattagtggagaaacttatggtgcaaagaataagcctagagtggaggagatttatccttggaagttggggactatttcaactaagaaagttaagaagtgtcagtttcattctgctaagaaagctttggttataaaggaagatacgatggtgttggtggctcctgtggggaaggctgttgtggagaagattactctgatggagatagttgaatctgagaagtctatggtggtggaggagtccgtttggtggggggggtttactcctatgagtttggtaattgtggatcctggggatgccccctatgcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 Jacalin

Protein Analysis

333

Amino Acids

35.52

Weight (kDa)

6.45

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000109)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11460 FvH4_1g20081 FvH4_1g21331 FvH4_1g22022 FvH4_1g27932 FvH4_2g04010 FvH4_2g07601 FvH4_2g09181 FvH4_2g18601 FvH4_2g24951 FvH4_2g27371 FvH4_3g15420 FvH4_3g26790 FvH4_3g26791 FvH4_3g34441 FvH4_3g34930 FvH4_3g45133 FvH4_4g02561 FvH4_4g05731 FvH4_4g05732 FvH4_4g06570 FvH4_4g09962 FvH4_4g13551 FvH4_4g13621 FvH4_4g14830 FvH4_4g15081 FvH4_4g26661 FvH4_5g05871 FvH4_5g08822 FvH4_5g12461 FvH4_5g24922 FvH4_5g30051 FvH4_5g30200 FvH4_5g30551 FvH4_5g32991 FvH4_5g36132 FvH4_5g37291 FvH4_6g07931 FvH4_6g08641 FvH4_6g12800 FvH4_6g13840 FvH4_6g13900 FvH4_6g16432 FvH4_6g16701 FvH4_6g28831 FvH4_6g31171 FvH4_6g41261 FvH4_7g00030 FvH4_7g01201 FvH4_7g08120 FvH4_7g10071 FvH4_7g18780
rosa_chinensis RchiOBHm_Chr2g0119561 RchiOBHm_Chr3g0458511 RchiOBHm_Chr3g0477731 RchiOBHm_Chr3g0479111 RchiOBHm_Chr3g0497091 RchiOBHm_Chr5g0072451 RchiOBHm_Chr6g0284681 RchiOBHm_Chr6g0311691 RchiOBHm_Chr7g0182191
rosa_laevigata RLG00000000983 RLG00000001598 RLG00000004616 RLG00000004931 RLG00000006806 RLG00000008371 RLG00000009544 RLG00000009718 RLG00000013465 RLG00000013533 RLG00000014631 RLG00000014987 RLG00000015588 RLG00000016226 RLG00000018171 RLG00000018433 RLG00000018649 RLG00000018710 RLG00000018870 RLG00000019205 RLG00000020638 RLG00000023424 RLG00000024707 RLG00000025967 RLG00000030020 RLG00000030596 RLG00000030996 RLG00000033451 RLG00000033757 RLG00000033758 RLG00000033808 RLG00000033812 RLG00000034857 RLG00000035240 RLG00000035691 RLG00000036232 RLG00000036301 RLG00000036412
rosa_multiflora Rmu_co8113544.1_g000001 Rmu_co8372331.1_g000001 Rmu_sc0000298.1_g000003 Rmu_sc0000552.1_g000026 Rmu_sc0000814.1_g000037 Rmu_sc0001211.1_g000126 Rmu_sc0001311.1_g000026 Rmu_sc0001354.1_g000009 Rmu_sc0002119.1_g000029 Rmu_sc0002357.1_g000020 Rmu_sc0002573.1_g000014 Rmu_sc0002586.1_g000022 Rmu_sc0003517.1_g000004 Rmu_sc0003558.1_g000001 Rmu_sc0004084.1_g000044 Rmu_sc0004143.1_g000011 Rmu_sc0004514.1_g000002 Rmu_sc0005370.1_g000004 Rmu_sc0005392.1_g000015 Rmu_sc0005664.1_g000009 Rmu_sc0006235.1_g000009 Rmu_sc0006423.1_g000001 Rmu_sc0006838.1_g000018 Rmu_sc0006847.1_g000005 Rmu_sc0006959.1_g000019 Rmu_sc0007111.1_g000002 Rmu_sc0009125.1_g000001 Rmu_sc0009546.1_g000015 Rmu_sc0009670.1_g000006 Rmu_sc0011618.1_g000004 Rmu_sc0012306.1_g000002 Rmu_sc0012306.1_g000003 Rmu_sc0012355.1_g000001 Rmu_sc0012355.1_g000002 Rmu_sc0013116.1_g000002 Rmu_sc0016872.1_g000001 Rmu_sc0017043.1_g000005 Rmu_sc0017081.1_g000003 Rmu_sc0018075.1_g000002 Rmu_sc0020578.1_g000002 Rmu_sc0034515.1_g000001 Rmu_sc0040072.1_g000003 Rmu_ssc0000309.1_g000056 Rmu_ssc0000330.1_g000048
rosa_roxburghii Rroxscaffold_1G00007410 Rroxscaffold_1G00027350 Rroxscaffold_1G00043810 Rroxscaffold_2G00099880 Rroxscaffold_2G00112650 Rroxscaffold_2G00122020 Rroxscaffold_3G00223350 Rroxscaffold_3G00239670 Rroxscaffold_5G00333740 Rroxscaffold_5G00335340 Rroxscaffold_5G00341030 Rroxscaffold_5G00342730 Rroxscaffold_6G00426030 Rroxscaffold_7G00162470 Rroxscaffold_7G00196110 Rroxscaffold_7G00202440 Rroxscaffold_7G00202450 Rroxscaffold_7G00215740
rosa_rugosa Rorug01G0176600 Rorug01G0276600 Rorug02G0236500 Rorug02G0277000 Rorug02G0363300 Rorug02G0375900 Rorug02G0473700 Rorug02G0546200 Rorug02G0601000 Rorug03G0158400 Rorug03G0289500 Rorug04G0026600 Rorug04G0090000 Rorug04G0105500 Rorug04G0263100 Rorug04G0394500 Rorug05G0122200 Rorug05G0152200 Rorug05G0541800 Rorug05G0551100 Rorug05G0554400 Rorug06G0244900 Rorug06G0401400
rosa_samantha Rh1CG158000 Rh1CG280800 Rh1CG326900 Rh2BG051500 Rh2BG101500 Rh2BG438200 Rh2BG509100 Rh2BG531600 Rh2BG531800 Rh2BG629800 Rh2BG629900 Rh2CG565300 Rh2DG448000 Rh2DG540600 Rh2DG540700 Rh2DG641700 Rh2DG641800 Rh3AG222200 Rh3BG034200 Rh3BG254700 Rh3DG286800 Rh3DG322600 Rh4AG009200 Rh4AG050400 Rh4AG223100 Rh4BG072500 Rh4BG106000 Rh4CG010200 Rh4CG053700 Rh4CG236100 Rh4CG236200 Rh4CG236300 Rh4DG104800 Rh5AG474800 Rh5BG214500 Rh5BG360000 Rh5BG403700 Rh5BG494900 Rh5DG255300 Rh6AG205400 Rh6BG227300 Rh6CG280700 Rh6DG273400 Rh7AG187300 Rh7AG220800 Rh7AG355700 Rh7AG462000 Rh7AG462200 Rh7AG465900 Rh7CG198300 Rh7CG222100 Rh7CG373000 Rh7CG479300 Rh7DG233800
rosa_wichuraiana Rw0G018350 Rw1G026500 Rw2G024750 Rw2G051200 Rw5G001570 Rw5G036850 Rw6G016070 Rw6G042710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
AasI GACNNNNNNGTC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 373
AciI CCGC 2 cut(s) 467, 536
AclWI GGATC 2 cut(s) 971, 984
AcoI YGGCCR 1 cut(s) 690
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 939
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 3 cut(s) 344, 771, 902
AjiI CACGTC 1 cut(s) 158
AjnI CCWGG 1 cut(s) 979
AluBI AGCT 3 cut(s) 29, 347, 815
AluI AGCT 3 cut(s) 29, 347, 815
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 3 cut(s) 28, 323, 355
AlwI GGATC 2 cut(s) 971, 984
AoxI GGCC 1 cut(s) 690
ApeKI GCWGC 2 cut(s) 258, 524
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspA2I CCTAGG 1 cut(s) 455
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 1 cut(s) 683
AvaII GGWCC 1 cut(s) 209
AvrII CCTAGG 1 cut(s) 455
AxyI CCTNAGG 1 cut(s) 519
BaeGI GKGCMC 1 cut(s) 415
BaeI ACNNNNGTAYC 2 cut(s) 825, 858
BamHI GGATCC 1 cut(s) 976
BanI GGYRCC 1 cut(s) 373
BauI CACGAG 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 245, 511
BccI CCATC 4 cut(s) 4, 580, 832, 883
BceAI ACGGC 1 cut(s) 271
BciT130I CCWGG 1 cut(s) 981
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 3 cut(s) 28, 323, 355
BfaI CTAG 2 cut(s) 456, 729
BglII AGATCT 1 cut(s) 195
BisI GCNGC 2 cut(s) 259, 525
BlnI CCTAGG 1 cut(s) 455
BlsI GCNGC 2 cut(s) 260, 526
Bme1390I CCNGG 2 cut(s) 669, 981
Bme18I GGWCC 1 cut(s) 209
BmgBI CACGTC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 4 cut(s) 375, 591, 852, 978
BmrFI CCNGG 2 cut(s) 669, 981
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 3 cut(s) 504, 608, 976
BmuI ACTGGG 1 cut(s) 417
BplI GAGNNNNNCTC 2 cut(s) 8, 40
BpuMI CCSGG 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 28
BsaJI CCNNGG 4 cut(s) 212, 455, 749, 980
Bsc4I CCNNNNNNNGG 1 cut(s) 939
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 1 cut(s) 412
Bse21I CCTNAGG 1 cut(s) 519
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 1 cut(s) 981
BseDI CCNNGG 4 cut(s) 212, 455, 749, 980
BseGI GGATG 1 cut(s) 991
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 939
BseMII CTCAG 3 cut(s) 39, 533, 897
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 4 cut(s) 32, 668, 752, 941
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 2 cut(s) 245, 511
BshFI GGCC 1 cut(s) 692
BshNI GGYRCC 1 cut(s) 373
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 2 cut(s) 669, 689
BslFI GGGAC 1 cut(s) 775
BslI CCNNNNNNNGG 1 cut(s) 939
BsmAI GTCTC 3 cut(s) 28, 323, 355
BsmFI GGGAC 1 cut(s) 775
BsnI GGCC 1 cut(s) 692
Bso31I GGTCTC 1 cut(s) 28
Bsp1286I GDGCHC 2 cut(s) 415, 667
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
BspACI CCGC 2 cut(s) 467, 536
BspANI GGCC 1 cut(s) 692
BspCNI CTCAG 3 cut(s) 38, 532, 898
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 4 cut(s) 375, 591, 852, 978
BspPI GGATC 2 cut(s) 971, 984
BspT107I GGYRCC 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 28
BsrFI RCCGGY 1 cut(s) 688
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 412
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 4 cut(s) 212, 455, 749, 980
BssMI GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
BssSI CACGAG 1 cut(s) 471
BssT1I CCWWGG 2 cut(s) 455, 749
Bst2BI CACGAG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 981
Bst4CI ACNGT 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 88, 237, 690
BstDEI CTNAG 5 cut(s) 25, 519, 774, 807, 906
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 991
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 8 cut(s) 102, 198, 294, 300, 339, 656, 676, 979
BstMAI GTCTC 3 cut(s) 28, 323, 355
BstMBI GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
BstMWI GCNNNNNNNGC 4 cut(s) 35, 176, 812, 995
BstNI CCWGG 1 cut(s) 981
BstNSI RCATGY 3 cut(s) 422, 546, 556
BstSCI CCNGG 2 cut(s) 667, 979
BstSLI GKGCMC 1 cut(s) 415
BstV1I GCAGC 2 cut(s) 245, 511
BstX2I RGATCY 2 cut(s) 195, 976
BstYI RGATCY 2 cut(s) 195, 976
Bsu36I CCTNAGG 1 cut(s) 519
BsuRI GGCC 1 cut(s) 692
BtgI CCRYGG 1 cut(s) 212
BtgZI GCGATG 1 cut(s) 558
BtrI CACGTC 1 cut(s) 158
BtsCI GGATG 1 cut(s) 991
Cac8I GCNNGC 3 cut(s) 88, 237, 690
CciI TCATGA 1 cut(s) 402
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 209
Csp6I GTAC 1 cut(s) 4
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 5 cut(s) 25, 519, 774, 807, 906
DpnI GATC 8 cut(s) 101, 197, 293, 299, 338, 655, 675, 978
DpnII GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
Eco130I CCWWGG 2 cut(s) 455, 749
Eco31I GGTCTC 1 cut(s) 28
Eco32I GATATC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 209
Eco81I CCTNAGG 1 cut(s) 519
EcoRII CCWGG 1 cut(s) 979
EcoRV GATATC 1 cut(s) 624
EcoT14I CCWWGG 2 cut(s) 455, 749
ErhI CCWWGG 2 cut(s) 455, 749
FaqI GGGAC 1 cut(s) 775
FauI CCCGC 1 cut(s) 474
FbaI TGATCA 1 cut(s) 336
Fnu4HI GCNGC 2 cut(s) 259, 525
FokI GGATG 1 cut(s) 998
Fsp4HI GCNGC 2 cut(s) 259, 525
FspBI CTAG 2 cut(s) 456, 729
GlaI GCGC 1 cut(s) 45
GluI GCNGC 2 cut(s) 259, 525
HaeIII GGCC 1 cut(s) 692
HapII CCGG 2 cut(s) 669, 689
HhaI GCGC 1 cut(s) 46
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 1 cut(s) 813
HinfI GANTC 3 cut(s) 152, 902, 929
HpaII CCGG 2 cut(s) 669, 689
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 1 cut(s) 951
Hpy188I TCNGA 3 cut(s) 206, 888, 907
Hpy188III TCNNGA 3 cut(s) 295, 390, 403
Hpy8I GTNNAC 1 cut(s) 951
HpyAV CCTTC 3 cut(s) 588, 603, 856
HpyCH4III ACNGT 1 cut(s) 213
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 3 cut(s) 119, 599, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 176, 812, 995
HpyF3I CTNAG 5 cut(s) 25, 519, 774, 807, 906
HpySE526I ACGT 1 cut(s) 157
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 688
KroNI GCCGGC 1 cut(s) 690
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
LmnI GCTCC 2 cut(s) 670, 856
Lsp1109I GCAGC 2 cut(s) 245, 511
LweI GCATC 3 cut(s) 504, 608, 976
MaeI CTAG 2 cut(s) 456, 729
MaeII ACGT 1 cut(s) 157
MaeIII GTNAC 1 cut(s) 135
MalI GATC 8 cut(s) 101, 197, 293, 299, 338, 655, 675, 978
MboI GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
MboII GAAGA 4 cut(s) 78, 154, 842, 889
MfeI CAATTG 1 cut(s) 120
MflI RGATCY 2 cut(s) 195, 976
MhlI GDGCHC 2 cut(s) 415, 667
MluCI AATT 3 cut(s) 120, 636, 969
MlyI GAGTC 1 cut(s) 938
MroNI GCCGGC 1 cut(s) 688
MseI TTAA 1 cut(s) 783
MslI CAYNNNNRTG 2 cut(s) 11, 114
MspI CCGG 2 cut(s) 669, 689
MspR9I CCNGG 2 cut(s) 669, 981
MunI CAATTG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 981
MwoI GCNNNNNNNGC 4 cut(s) 35, 176, 812, 995
NaeI GCCGGC 1 cut(s) 690
NciI CCSGG 1 cut(s) 669
NdeII GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
NgoMIV GCCGGC 1 cut(s) 688
NlaIV GGNNCC 4 cut(s) 375, 591, 852, 978
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 1 cut(s) 135
NspI RCATGY 3 cut(s) 422, 546, 556
PagI TCATGA 1 cut(s) 402
PciI ACATGT 1 cut(s) 542
PdiI GCCGGC 1 cut(s) 690
PfeI GAWTC 2 cut(s) 152, 902
PkrI GCNGC 2 cut(s) 260, 526
PleI GAGTC 1 cut(s) 937
PpsI GAGTC 1 cut(s) 937
PscI ACATGT 1 cut(s) 542
PsiI TTATAA 1 cut(s) 824
Psp6I CCWGG 1 cut(s) 979
PspGI CCWGG 1 cut(s) 979
PspN4I GGNNCC 4 cut(s) 375, 591, 852, 978
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 2 cut(s) 195, 976
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 11, 114
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 2 cut(s) 259, 525
Sau3AI GATC 8 cut(s) 99, 195, 291, 297, 336, 653, 673, 976
Sau96I GGNCC 1 cut(s) 209
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 2 cut(s) 669, 981
SduI GDGCHC 2 cut(s) 415, 667
SetI ASST 8 cut(s) 24, 31, 160, 349, 402, 441, 461, 817
SfaNI GCATC 3 cut(s) 504, 608, 976
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 2 cut(s) 11, 114
Sse9I AATT 3 cut(s) 120, 636, 969
SsiI CCGC 2 cut(s) 467, 536
SspMI CTAG 2 cut(s) 456, 729
StyD4I CCNGG 2 cut(s) 667, 979
StyI CCWWGG 2 cut(s) 455, 749
TaaI ACNGT 1 cut(s) 213
TaiI ACGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 296, 500, 652
TasI AATT 3 cut(s) 120, 636, 969
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 152, 902
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 2 cut(s) 258, 524
Tsp45I GTSAC 1 cut(s) 135
TspDTI ATGAA 3 cut(s) 154, 198, 788
TspGWI ACGGA 3 cut(s) 220, 410, 922
VpaK11BI GGWCC 1 cut(s) 209
XceI RCATGY 3 cut(s) 422, 546, 556
XmaJI CCTAGG 1 cut(s) 455
XspI CTAG 2 cut(s) 456, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.