Rorug02G0473700

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000109
Physical Location & Seq
Reverse (-)
60522501 .. 60526561
4061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0473700.1

Sequence Viewer

Length: 2040 bp
ATGGGTACCGCCTATAAACCGAAGAACATCCTCATTACTGGAGCTGCTGGCTTCATCGCATCCCATGTTTGCAATCGGCTTATCAGGAACTACCCTGAATACAAGATTGTCGTCCTTGACAAGCTTGATTACTGTTCAAATTTGAAGAACCTTCACCCCTCAAGGTCATCCTCAAACTTCAAATTTATCAAGGGAGACATTGGAAGCGCTGACCTTGTCAACTTCATCCTTCTCACCGAGTCCATTGATACAATAATGCACTTCGCTGCCCAGACCCATGTTGACAACTCATTTGGTAACAGCTTTGAGTTCACTAAAAACAACATATATGGCACGCATGTTCTTCTAGAAGCATGCAAAGTCACTGGCCAAATCAAAAGGTTCATCCATGTTAGCACAGATGAAGTTTATGGGGAGACAGATGAGGATGCTGTGGTGGGAAATCATGAGGCTTCTCAGCTTCTTCCCACAAACCCCTACTCTGCGACTAAAGCTGGAGCAGAGATGCTTGTTATGGCATATGGACGTTCATATGGGCTTCCTGTGATAACTACCAGGGGAAACAATGTTTATGGCCCCAATCAGTTCCCTGAAAAGATGATTCCAAAGTTCATGCTCTTGGCTATGAAAGGGAAGACTCTTCCGATTCACGGTGATGGATCAAATGTCAGGAGTTATCTCTATTGTGAGGATGTAGCCGAGGCATTTGAGGTCATTCTCCATAAGGGTGAGGTAGGCCATGTCTACAACATTGGGACAAAGAAAGAGAGGAGGGTAGTTGATGTCGCTAGGGAAATTTGCCAACTGTTCTCGTTGAACCCAGATACATATATAAACTTTGTTGAGAATAGACCTTTTAATGATCAGAGGTATTTTCTGGATGACCAAAAGCTTAAAAACTTGGGATGGTCTGAAAGTACTTCATGGGAGGAAGGTTTGAGGAAGACAATGGAATGGTACGTCAAGAATCCTCATTGGTGGGGAGATGTTTCTGGAGCACTGCTCCCTCATCCAAGAATGCTCATGGTTCCTGGAATTGAAAGAAACTTTGATGGGCCTGATACAAGCAATTCTGATTCCCCTGTGCCAGCAACTGATTCTAGTCAAAGCCAAGTGGTTGTTCCAGCTCCTAGGGGCATTCCATCTACTCAGAAGCCATCTCTGAAGTTCCTGATTTATGGGAGAACAGGGTGGATTGGAGGCCTTCTTGGGAAGATTTGTGAGAAACAAGGGATACCGTTTGAGTATGGACGAGGGCGGCTCCAGGAACGTTCTCAGCTCTTGGCTGATATTCAGAGGGTTAAGCCAACCCATGTTTTCAATGCTGCTGGAGTGACTGGCAGACCCAATGTGGATTGGTGTGAATCTCATAAACCAGAAACAATTCGGACCAATGTTGTGGGTACATTGACCTTGGCTGATGTCTGCAGAGAGCACAACCTCCTAATGATGAATTATGCTACTGGTTGTATCTTCGAGTACGATGCAGCTCATCCATCAAGATCAGGAATTGGGTTCAAGGAGGAAGATACACCAAATTTCACTGGTTCATTTTACTCCAAAACCAAAGCCATGGTGGAAGAGCTTTTGAAAGAATATGACAATGTCTGCACTCTTAGAGTTCGAATGCCTATATCATCTGATCTCAGCAATCCACGCAACTTCATCACAAAGATCTCCAAGTACAACAAAGTGGTTGATATTCCAAACAGCATGACTATCTTGGATGAACTTCTACCGATTTCAGTTGAGATGGCCAAGAGGAACTTGAAGGGCATTTGGAACTTCACAAACCCTGGTGTTGTGAGTCACAATGAGATCCTGGAGATGTACAAGAAGTACATAGACCCCAGTTTCAAGTGGGTTAATTTTACATTGGAAGAACAAGCCAAGGTTATTGTGGCCCCAAGAAGTAACAATGAGATGGATGCATCTAAGTTGAAGAAAGAGTTCCCTGAACTATTGCCAATCAAGGAGTCGCTGATTAAATACGTCTTTGAGCCCAACAAGAAAACATTTGCTGGTGGAGCAGCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

679

Amino Acids

76.42

Weight (kDa)

7.59

Isoelectric Point (pI)

32.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 9 - 318 9.6e-14 RmlD substrate binding domain
Epimerase PF01370 10 - 252 3.9e-56 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 10 - 280 2.9e-20 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 11 - 316 2e-65 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 11 - 236 1.4e-24 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 12 - 195 3.1e-19 Male sterility protein
RmlD_sub_bind PF04321 389 - 558 3.9e-11 RmlD substrate binding domain
Epimerase PF01370 418 - 553 5.7e-11 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000109)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11460 FvH4_1g20081 FvH4_1g21331 FvH4_1g22022 FvH4_1g27932 FvH4_2g04010 FvH4_2g07601 FvH4_2g09181 FvH4_2g18601 FvH4_2g24951 FvH4_2g27371 FvH4_3g15420 FvH4_3g26790 FvH4_3g26791 FvH4_3g34441 FvH4_3g34930 FvH4_3g45133 FvH4_4g02561 FvH4_4g05731 FvH4_4g05732 FvH4_4g06570 FvH4_4g09962 FvH4_4g13551 FvH4_4g13621 FvH4_4g14830 FvH4_4g15081 FvH4_4g26661 FvH4_5g05871 FvH4_5g08822 FvH4_5g12461 FvH4_5g24922 FvH4_5g30051 FvH4_5g30200 FvH4_5g30551 FvH4_5g32991 FvH4_5g36132 FvH4_5g37291 FvH4_6g07931 FvH4_6g08641 FvH4_6g12800 FvH4_6g13840 FvH4_6g13900 FvH4_6g16432 FvH4_6g16701 FvH4_6g28831 FvH4_6g31171 FvH4_6g41261 FvH4_7g00030 FvH4_7g01201 FvH4_7g08120 FvH4_7g10071 FvH4_7g18780
rosa_chinensis RchiOBHm_Chr2g0119561 RchiOBHm_Chr3g0458511 RchiOBHm_Chr3g0477731 RchiOBHm_Chr3g0479111 RchiOBHm_Chr3g0497091 RchiOBHm_Chr5g0072451 RchiOBHm_Chr6g0284681 RchiOBHm_Chr6g0311691 RchiOBHm_Chr7g0182191
rosa_laevigata RLG00000000983 RLG00000001598 RLG00000004616 RLG00000004931 RLG00000006806 RLG00000008371 RLG00000009544 RLG00000009718 RLG00000013465 RLG00000013533 RLG00000014631 RLG00000014987 RLG00000015588 RLG00000016226 RLG00000018171 RLG00000018433 RLG00000018649 RLG00000018710 RLG00000018870 RLG00000019205 RLG00000020638 RLG00000023424 RLG00000024707 RLG00000025967 RLG00000030020 RLG00000030596 RLG00000030996 RLG00000033451 RLG00000033757 RLG00000033758 RLG00000033808 RLG00000033812 RLG00000034857 RLG00000035240 RLG00000035691 RLG00000036232 RLG00000036301 RLG00000036412
rosa_multiflora Rmu_co8113544.1_g000001 Rmu_co8372331.1_g000001 Rmu_sc0000298.1_g000003 Rmu_sc0000552.1_g000026 Rmu_sc0000814.1_g000037 Rmu_sc0001211.1_g000126 Rmu_sc0001311.1_g000026 Rmu_sc0001354.1_g000009 Rmu_sc0002119.1_g000029 Rmu_sc0002357.1_g000020 Rmu_sc0002573.1_g000014 Rmu_sc0002586.1_g000022 Rmu_sc0003517.1_g000004 Rmu_sc0003558.1_g000001 Rmu_sc0004084.1_g000044 Rmu_sc0004143.1_g000011 Rmu_sc0004514.1_g000002 Rmu_sc0005370.1_g000004 Rmu_sc0005392.1_g000015 Rmu_sc0005664.1_g000009 Rmu_sc0006235.1_g000009 Rmu_sc0006423.1_g000001 Rmu_sc0006838.1_g000018 Rmu_sc0006847.1_g000005 Rmu_sc0006959.1_g000019 Rmu_sc0007111.1_g000002 Rmu_sc0009125.1_g000001 Rmu_sc0009546.1_g000015 Rmu_sc0009670.1_g000006 Rmu_sc0011618.1_g000004 Rmu_sc0012306.1_g000002 Rmu_sc0012306.1_g000003 Rmu_sc0012355.1_g000001 Rmu_sc0012355.1_g000002 Rmu_sc0013116.1_g000002 Rmu_sc0016872.1_g000001 Rmu_sc0017043.1_g000005 Rmu_sc0017081.1_g000003 Rmu_sc0018075.1_g000002 Rmu_sc0020578.1_g000002 Rmu_sc0034515.1_g000001 Rmu_sc0040072.1_g000003 Rmu_ssc0000309.1_g000056 Rmu_ssc0000330.1_g000048
rosa_roxburghii Rroxscaffold_1G00007410 Rroxscaffold_1G00027350 Rroxscaffold_1G00043810 Rroxscaffold_2G00099880 Rroxscaffold_2G00112650 Rroxscaffold_2G00122020 Rroxscaffold_3G00223350 Rroxscaffold_3G00239670 Rroxscaffold_5G00333740 Rroxscaffold_5G00335340 Rroxscaffold_5G00341030 Rroxscaffold_5G00342730 Rroxscaffold_6G00426030 Rroxscaffold_7G00162470 Rroxscaffold_7G00196110 Rroxscaffold_7G00202440 Rroxscaffold_7G00202450 Rroxscaffold_7G00215740
rosa_rugosa Rorug01G0176600 Rorug01G0276600 Rorug02G0236500 Rorug02G0277000 Rorug02G0363300 Rorug02G0375900 Rorug02G0473700 Rorug02G0546200 Rorug02G0601000 Rorug03G0158400 Rorug03G0289500 Rorug04G0026600 Rorug04G0090000 Rorug04G0105500 Rorug04G0263100 Rorug04G0394500 Rorug05G0122200 Rorug05G0152200 Rorug05G0541800 Rorug05G0551100 Rorug05G0554400 Rorug06G0244900 Rorug06G0401400
rosa_samantha Rh1CG158000 Rh1CG280800 Rh1CG326900 Rh2BG051500 Rh2BG101500 Rh2BG438200 Rh2BG509100 Rh2BG531600 Rh2BG531800 Rh2BG629800 Rh2BG629900 Rh2CG565300 Rh2DG448000 Rh2DG540600 Rh2DG540700 Rh2DG641700 Rh2DG641800 Rh3AG222200 Rh3BG034200 Rh3BG254700 Rh3DG286800 Rh3DG322600 Rh4AG009200 Rh4AG050400 Rh4AG223100 Rh4BG072500 Rh4BG106000 Rh4CG010200 Rh4CG053700 Rh4CG236100 Rh4CG236200 Rh4CG236300 Rh4DG104800 Rh5AG474800 Rh5BG214500 Rh5BG360000 Rh5BG403700 Rh5BG494900 Rh5DG255300 Rh6AG205400 Rh6BG227300 Rh6CG280700 Rh6DG273400 Rh7AG187300 Rh7AG220800 Rh7AG355700 Rh7AG462000 Rh7AG462200 Rh7AG465900 Rh7CG198300 Rh7CG222100 Rh7CG373000 Rh7CG479300 Rh7DG233800
rosa_wichuraiana Rw0G018350 Rw1G026500 Rw2G024750 Rw2G051200 Rw5G001570 Rw5G036850 Rw6G016070 Rw6G042710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AccI GTMKAC 1 cut(s) 744
AciI CCGC 2 cut(s) 9, 1258
AclI AACGTT 1 cut(s) 1270
AclWI GGATC 2 cut(s) 667, 1813
AcoI YGGCCR 2 cut(s) 367, 1755
AcsI RAATTY 4 cut(s) 139, 182, 795, 1537
AcuI CTGAAG 1 cut(s) 1184
AfaI GTAC 8 cut(s) 7, 919, 959, 1405, 1481, 1685, 1832, 1841
AfeI AGCGCT 1 cut(s) 208
AfiI CCNNNNNNNGG 1 cut(s) 650
AjnI CCWGG 5 cut(s) 554, 1030, 1263, 1795, 1821
AjuI GAANNNNNNNTTGG 2 cut(s) 1104, 1136
Alw21I GWGCWC 2 cut(s) 1000, 1437
Alw26I GTCTC 2 cut(s) 189, 410
AlwI GGATC 2 cut(s) 667, 1813
AlwNI CAGNNNCTG 1 cut(s) 1094
Aor51HI AGCGCT 1 cut(s) 208
AoxI GGCC 7 cut(s) 367, 574, 736, 1055, 1201, 1755, 1902
ApeKI GCWGC 5 cut(s) 44, 266, 1325, 1487, 2030
ApoI RAATTY 4 cut(s) 139, 182, 795, 1537
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
Asp700I GAANNNNTTC 2 cut(s) 1383, 1949
Asp718I GGTACC 1 cut(s) 5
AspA2I CCTAGG 1 cut(s) 1130
AspLEI GCGC 1 cut(s) 209
AspS9I GGNCC 4 cut(s) 575, 1055, 1389, 1903
AsuHPI GGTGA 4 cut(s) 146, 226, 665, 740
AsuII TTCGAA 1 cut(s) 1624
AvaII GGWCC 1 cut(s) 1389
AvrII CCTAGG 1 cut(s) 1130
BalI TGGCCA 2 cut(s) 369, 1757
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 2004
BbsI GAAGAC 2 cut(s) 641, 950
Bbv12I GWGCWC 2 cut(s) 1000, 1437
BbvI GCAGC 4 cut(s) 31, 253, 1312, 1499
BccI CCATC 8 cut(s) 650, 900, 1046, 1150, 1165, 1504, 1747, 1918
BcgI CGANNNNNNTGC 2 cut(s) 1466, 1500
BciT130I CCWGG 5 cut(s) 556, 1032, 1265, 1797, 1823
BciVI GTATCC 1 cut(s) 1227
BclI TGATCA 1 cut(s) 862
BcoDI GTCTC 2 cut(s) 189, 410
BfaI CTAG 4 cut(s) 347, 789, 1101, 1131
BfmI CTRYAG 1 cut(s) 1426
BfoI RGCGCY 1 cut(s) 210
BfuI GTATCC 1 cut(s) 1227
BglII AGATCT 1 cut(s) 1674
BisI GCNGC 6 cut(s) 45, 267, 1259, 1326, 1488, 2031
BlnI CCTAGG 1 cut(s) 1130
BlsI GCNGC 6 cut(s) 46, 268, 1260, 1327, 1489, 2032
BmcAI AGTACT 1 cut(s) 919
Bme1390I CCNGG 5 cut(s) 556, 1032, 1265, 1797, 1823
Bme18I GGWCC 1 cut(s) 1389
BmgT120I GGNCC 4 cut(s) 575, 1055, 1389, 1903
BmiI GGNNCC 5 cut(s) 7, 577, 1029, 1262, 1905
BmrFI CCNGG 5 cut(s) 556, 1032, 1265, 1797, 1823
BmrI ACTGGG 1 cut(s) 1845
BmsI GCATC 6 cut(s) 68, 418, 495, 1474, 1918, 1940
BmuI ACTGGG 1 cut(s) 1845
BpiI GAAGAC 2 cut(s) 641, 950
BplI GAGNNNNNCTC 4 cut(s) 987, 1019, 1245, 1277
BpmI CTGGAG 6 cut(s) 60, 516, 1014, 1247, 1350, 1844
Bpu14I TTCGAA 1 cut(s) 1624
BpuEI CTTGAG 1 cut(s) 145
BsaJI CCNNGG 7 cut(s) 555, 699, 1130, 1413, 1572, 1795, 1890
Bsc4I CCNNNNNNNGG 1 cut(s) 650
Bse1I ACTGG 6 cut(s) 43, 370, 1342, 1468, 1549, 1851
BseBI CCWGG 5 cut(s) 556, 1032, 1265, 1797, 1823
BseDI CCNNGG 7 cut(s) 555, 699, 1130, 1413, 1572, 1795, 1890
BseLI CCNNNNNNNGG 1 cut(s) 650
BseMII CTCAG 4 cut(s) 470, 1163, 1289, 1660
BseNI ACTGG 6 cut(s) 43, 370, 1342, 1468, 1549, 1851
BseRI GAGGAG 1 cut(s) 784
BseXI GCAGC 4 cut(s) 31, 253, 1312, 1499
BsgI GTGCAG 1 cut(s) 1594
BshFI GGCC 7 cut(s) 369, 576, 738, 1057, 1203, 1757, 1904
BshNI GGYRCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 1000, 1437
BslFI GGGAC 1 cut(s) 769
BslI CCNNNNNNNGG 1 cut(s) 650
BsmAI GTCTC 2 cut(s) 189, 410
BsmFI GGGAC 1 cut(s) 769
BsmI GAATGC 3 cut(s) 1023, 1137, 1632
BsnI GGCC 7 cut(s) 369, 576, 738, 1057, 1203, 1757, 1904
Bsp119I TTCGAA 1 cut(s) 1624
Bsp1286I GDGCHC 3 cut(s) 1000, 1437, 2004
Bsp1407I TGTACA 1 cut(s) 1830
Bsp143I GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
Bsp19I CCATGG 1 cut(s) 1572
BspACI CCGC 2 cut(s) 9, 1258
BspANI GGCC 7 cut(s) 369, 576, 738, 1057, 1203, 1757, 1904
BspCNI CTCAG 4 cut(s) 469, 1162, 1288, 1659
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 5 cut(s) 7, 577, 1029, 1262, 1905
BspMAI CTGCAG 1 cut(s) 1430
BspPI GGATC 2 cut(s) 667, 1813
BspQI GCTCTTC 1 cut(s) 1575
BspT104I TTCGAA 1 cut(s) 1624
BspT107I GGYRCC 1 cut(s) 5
BsrGI TGTACA 1 cut(s) 1830
BsrI ACTGG 6 cut(s) 43, 370, 1342, 1468, 1549, 1851
BssECI CCNNGG 7 cut(s) 555, 699, 1130, 1413, 1572, 1795, 1890
BssMI GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
BssT1I CCWWGG 4 cut(s) 1130, 1413, 1572, 1890
Bst2UI CCWGG 5 cut(s) 556, 1032, 1265, 1797, 1823
Bst4CI ACNGT 4 cut(s) 134, 653, 807, 1239
Bst6I CTCTTC 2 cut(s) 645, 1575
BstAUI TGTACA 1 cut(s) 1830
BstBI TTCGAA 1 cut(s) 1624
BstC8I GCNNGC 4 cut(s) 49, 335, 355, 1089
BstDEI CTNAG 6 cut(s) 456, 1149, 1275, 1616, 1646, 1935
BstDSI CCRYGG 1 cut(s) 1572
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 6 cut(s) 662, 865, 1505, 1645, 1677, 1821
BstMAI GTCTC 2 cut(s) 189, 410
BstMBI GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
BstMWI GCNNNNNNNGC 3 cut(s) 491, 1656, 2027
BstNI CCWGG 5 cut(s) 556, 1032, 1265, 1797, 1823
BstNSI RCATGY 2 cut(s) 341, 357
BstSCI CCNGG 5 cut(s) 554, 1030, 1263, 1795, 1821
BstSFI CTRYAG 1 cut(s) 1426
BstV1I GCAGC 4 cut(s) 31, 253, 1312, 1499
BstV2I GAAGAC 2 cut(s) 641, 950
BstX2I RGATCY 2 cut(s) 1674, 1818
BstXI CCANNNNNNTGG 2 cut(s) 1399, 1573
BstYI RGATCY 2 cut(s) 1674, 1818
BsuI GTATCC 1 cut(s) 1227
BsuRI GGCC 7 cut(s) 369, 576, 738, 1057, 1203, 1757, 1904
BtgI CCRYGG 1 cut(s) 1572
BtgZI GCGATG 1 cut(s) 40
BtsI GCAGTG 1 cut(s) 998
BtsIMutI CAGTG 3 cut(s) 363, 998, 1542
Cac8I GCNNGC 4 cut(s) 49, 335, 355, 1089
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 1 cut(s) 445
CfoI GCGC 1 cut(s) 209
Cfr13I GGNCC 4 cut(s) 575, 1055, 1389, 1903
Csp6I GTAC 8 cut(s) 6, 918, 958, 1404, 1480, 1684, 1831, 1840
CviQI GTAC 8 cut(s) 6, 918, 958, 1404, 1480, 1684, 1831, 1840
DdeI CTNAG 6 cut(s) 456, 1149, 1275, 1616, 1646, 1935
DpnI GATC 6 cut(s) 661, 864, 1504, 1644, 1676, 1820
DpnII GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
EaeI YGGCCR 2 cut(s) 367, 1755
Eam1104I CTCTTC 2 cut(s) 645, 1575
EarI CTCTTC 2 cut(s) 645, 1575
Eco130I CCWWGG 4 cut(s) 1130, 1413, 1572, 1890
Eco147I AGGCCT 1 cut(s) 1203
Eco24I GRGCYC 1 cut(s) 2004
Eco47I GGWCC 1 cut(s) 1389
Eco47III AGCGCT 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 1184
EcoRII CCWGG 5 cut(s) 554, 1030, 1263, 1795, 1821
EcoT14I CCWWGG 4 cut(s) 1130, 1413, 1572, 1890
EcoT22I ATGCAT 1 cut(s) 1933
EcoT38I GRGCYC 1 cut(s) 2004
ErhI CCWWGG 4 cut(s) 1130, 1413, 1572, 1890
FalI AAGNNNNNCTT 2 cut(s) 1751, 1783
FaqI GGGAC 1 cut(s) 769
FauNDI CATATG 2 cut(s) 520, 532
FbaI TGATCA 1 cut(s) 862
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 6 cut(s) 45, 267, 1259, 1326, 1488, 2031
FriOI GRGCYC 1 cut(s) 2004
Fsp4HI GCNGC 6 cut(s) 45, 267, 1259, 1326, 1488, 2031
FspBI CTAG 4 cut(s) 347, 789, 1101, 1131
GlaI GCGC 1 cut(s) 208
GluI GCNGC 6 cut(s) 45, 267, 1259, 1326, 1488, 2031
GsuI CTGGAG 6 cut(s) 60, 516, 1014, 1247, 1350, 1844
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 7 cut(s) 369, 576, 738, 1057, 1203, 1757, 1904
HhaI GCGC 1 cut(s) 209
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HincII GTYRAC 2 cut(s) 220, 283
HindII GTYRAC 2 cut(s) 220, 283
HindIII AAGCTT 2 cut(s) 122, 890
HphI GGTGA 4 cut(s) 146, 226, 665, 740
Hpy166II GTNNAC 4 cut(s) 220, 283, 312, 745
Hpy188I TCNGA 9 cut(s) 645, 867, 913, 1075, 1152, 1164, 1296, 1389, 1642
Hpy8I GTNNAC 4 cut(s) 220, 283, 312, 745
HpyAV CCTTC 5 cut(s) 161, 239, 926, 1214, 1765
HpyCH4III ACNGT 4 cut(s) 134, 653, 807, 1239
HpyCH4IV ACGT 4 cut(s) 526, 960, 1270, 1992
HpyCH4V TGCA 7 cut(s) 72, 259, 357, 1428, 1487, 1611, 1931
HpyF10VI GCNNNNNNNGC 3 cut(s) 491, 1656, 2027
HpyF3I CTNAG 6 cut(s) 456, 1149, 1275, 1616, 1646, 1935
HpySE526I ACGT 4 cut(s) 526, 960, 1270, 1992
HspAI GCGC 1 cut(s) 207
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
LguI GCTCTTC 1 cut(s) 1575
LmnI GCTCC 7 cut(s) 41, 497, 995, 1008, 1132, 1266, 2027
Lsp1109I GCAGC 4 cut(s) 31, 253, 1312, 1499
LweI GCATC 6 cut(s) 68, 418, 495, 1474, 1918, 1940
MaeI CTAG 4 cut(s) 347, 789, 1101, 1131
MaeII ACGT 4 cut(s) 526, 960, 1270, 1992
MaeIII GTNAC 5 cut(s) 296, 361, 1333, 1808, 1913
MalI GATC 6 cut(s) 661, 864, 1504, 1644, 1676, 1820
MboI GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
MflI RGATCY 2 cut(s) 1674, 1818
MhlI GDGCHC 3 cut(s) 1000, 1437, 2004
MlsI TGGCCA 2 cut(s) 369, 1757
MluNI TGGCCA 2 cut(s) 369, 1757
MlyI GAGTC 4 cut(s) 248, 631, 1816, 1985
Mox20I TGGCCA 2 cut(s) 369, 1757
Mph1103I ATGCAT 1 cut(s) 1933
MroXI GAANNNNTTC 2 cut(s) 1383, 1949
MscI TGGCCA 2 cut(s) 369, 1757
MseI TTAA 6 cut(s) 858, 894, 1302, 1866, 1986, 2038
MslI CAYNNNNRTG 2 cut(s) 654, 726
Msp20I TGGCCA 2 cut(s) 369, 1757
MspR9I CCNGG 5 cut(s) 556, 1032, 1265, 1797, 1823
Mva1269I GAATGC 3 cut(s) 1023, 1137, 1632
MvaI CCWGG 5 cut(s) 556, 1032, 1265, 1797, 1823
MwoI GCNNNNNNNGC 3 cut(s) 491, 1656, 2027
NcoI CCATGG 1 cut(s) 1572
NdeI CATATG 2 cut(s) 520, 532
NdeII GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
NlaIV GGNNCC 5 cut(s) 7, 577, 1029, 1262, 1905
NmeAIII GCCGAG 1 cut(s) 724
NmuCI GTSAC 3 cut(s) 361, 1333, 1808
NsiI ATGCAT 1 cut(s) 1933
NspI RCATGY 2 cut(s) 341, 357
NspV TTCGAA 1 cut(s) 1624
PaeI GCATGC 1 cut(s) 357
PagI TCATGA 1 cut(s) 445
PceI AGGCCT 1 cut(s) 1203
PciSI GCTCTTC 1 cut(s) 1575
PctI GAATGC 3 cut(s) 1023, 1137, 1632
PdmI GAANNNNTTC 2 cut(s) 1383, 1949
PfeI GAWTC 6 cut(s) 601, 646, 967, 1076, 1097, 1364
PflFI GACNNNGTC 2 cut(s) 215, 1604
PfoI TCCNGGA 3 cut(s) 1030, 1263, 1821
PkrI GCNGC 6 cut(s) 46, 268, 1260, 1327, 1489, 2032
PleI GAGTC 4 cut(s) 247, 631, 1815, 1984
PpsI GAGTC 4 cut(s) 247, 631, 1815, 1984
Psp1406I AACGTT 1 cut(s) 1270
Psp6I CCWGG 5 cut(s) 554, 1030, 1263, 1795, 1821
PspGI CCWGG 5 cut(s) 554, 1030, 1263, 1795, 1821
PspN4I GGNNCC 5 cut(s) 7, 577, 1029, 1262, 1905
PspPI GGNCC 4 cut(s) 575, 1055, 1389, 1903
PstI CTGCAG 1 cut(s) 1430
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 2 cut(s) 1674, 1818
PsyI GACNNNGTC 2 cut(s) 215, 1604
RsaI GTAC 8 cut(s) 7, 919, 959, 1405, 1481, 1685, 1832, 1841
RsaNI GTAC 8 cut(s) 6, 918, 958, 1404, 1480, 1684, 1831, 1840
RseI CAYNNNNRTG 2 cut(s) 654, 726
SapI GCTCTTC 1 cut(s) 1575
SaqAI TTAA 6 cut(s) 858, 894, 1302, 1866, 1986, 2038
SatI GCNGC 6 cut(s) 45, 267, 1259, 1326, 1488, 2031
Sau3AI GATC 6 cut(s) 659, 862, 1502, 1642, 1674, 1818
Sau96I GGNCC 4 cut(s) 575, 1055, 1389, 1903
ScaI AGTACT 1 cut(s) 919
SchI GAGTC 4 cut(s) 248, 631, 1816, 1985
ScrFI CCNGG 5 cut(s) 556, 1032, 1265, 1797, 1823
SduI GDGCHC 3 cut(s) 1000, 1437, 2004
SfaNI GCATC 6 cut(s) 68, 418, 495, 1474, 1918, 1940
SfcI CTRYAG 1 cut(s) 1426
SfuI TTCGAA 1 cut(s) 1624
SinI GGWCC 1 cut(s) 1389
SmiMI CAYNNNNRTG 2 cut(s) 654, 726
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
SphI GCATGC 1 cut(s) 357
SseBI AGGCCT 1 cut(s) 1203
SsiI CCGC 2 cut(s) 9, 1258
SspMI CTAG 4 cut(s) 347, 789, 1101, 1131
StuI AGGCCT 1 cut(s) 1203
StyD4I CCNGG 5 cut(s) 554, 1030, 1263, 1795, 1821
StyI CCWWGG 4 cut(s) 1130, 1413, 1572, 1890
TaaI ACNGT 4 cut(s) 134, 653, 807, 1239
TaiI ACGT 4 cut(s) 529, 963, 1273, 1995
TaqI TCGA 2 cut(s) 1476, 1624
TatI WGTACW 4 cut(s) 917, 1683, 1830, 1839
TauI GCSGC 1 cut(s) 1261
TfiI GAWTC 6 cut(s) 601, 646, 967, 1076, 1097, 1364
Tru1I TTAA 6 cut(s) 858, 894, 1302, 1866, 1986, 2038
Tru9I TTAA 6 cut(s) 858, 894, 1302, 1866, 1986, 2038
TscAI CASTG 3 cut(s) 370, 1005, 1549
TseFI GTSAC 3 cut(s) 361, 1333, 1808
TseI GCWGC 5 cut(s) 44, 266, 1325, 1487, 2030
Tsp45I GTSAC 3 cut(s) 361, 1333, 1808
TspRI CASTG 3 cut(s) 370, 1005, 1549
Tth111I GACNNNGTC 2 cut(s) 215, 1604
VpaK11BI GGWCC 1 cut(s) 1389
XapI RAATTY 4 cut(s) 139, 182, 795, 1537
XbaI TCTAGA 1 cut(s) 346
XceI RCATGY 2 cut(s) 341, 357
XcmI CCANNNNNNNNNTGG 2 cut(s) 1573, 1897
XmaJI CCTAGG 1 cut(s) 1130
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 2 cut(s) 1383, 1949
XspI CTAG 4 cut(s) 347, 789, 1101, 1131
ZrmI AGTACT 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 1933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.