Rorug05G0122200

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000109
Physical Location & Seq
Forward (+)
10802893 .. 10809123
6231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0122200.1

Sequence Viewer

Length: 2832 bp
ATGTTTTTTATGCACAGTAAAGATGATTCGGTGGTAGTCCCATCGAACTTTTTTAGTGAGATGCCGAAACTCAAGGTGCTGGATTTAACCAACATGTGTATGCCATCATTGCCGTTATCTCTTCAATACTTGAAGACGCTTCAAACATTGTGCTTAGATCTATGCACTCTGGGAGATATAACTCTACTCGGGCAGCTAAGTAGCTTAGAAATTCTTAGCCTTTTAAAATCCGATGTTGAAGAATTGCCTAGAGAAATAGGGCAATTGACTCGTCTTCGGTTGTTGGATTTATCTGAGTGTTCACAACTTGAAGTGATTTCACCTACTGTCATATCAAGCTTGGTGAGATTGGAAGATTTGAGAATGAGAAACAGCTTTAACAAATGGGAGGTTGAAGGTGAAAAAGGTAATGCAAGCCTGTCTGAGCTAAAGCACTTGTCTCAGCTATCCGCATTAGAAATACATATTCCGAATGCTGACTTTCTTCCAGCAAACTTGTTCTCCCACAAGTTAGAGAGATATAATATTTTTATTGGAGATGCGTGGGATGCGAGATATTCTTGGGATGAACCTATGGCTGCAGTGACCCTTAACACACTAAAACTCAAGCTCACTAACAACAATATTATGGGATTAGATGAAGGCCTAAAATCGTTGCTGAAGAGATCTGAGGACTTGTCCTTGGATGAGAGTGTTATTAATATTGTCCGGCAATTGGATACCAAAGATTTTGAGAATGTGAAACATCTCCGACTCCAAAACAATATTGACTTTACATATATCATTAATAGAAAGGTTGTATTCCCAAATCTAGTAACCTTGGAGGTGCATGGATGTGATAATTCAAGATTTTTGTTCTCATTTTCTATGGCTAAAAGTTTTATACTACTCAAACACCTGAAAATATCCACATGTGAACTTCTGCAAGAGATTGTATCATCAACAAGAGAATGCGCTGAAGAAAATATAGATGATGTGTTTTCTAAACTTAATATTTTGGAACTTGAAGCACTTCCAAACCTTGCTAGATTTGGCATAGCAAATTATATTGAATTTCCGGCCTTGGAAGAATTGGAGATAAAAGATTGTGTTAAATTGGTGGAATTCATTGGCGCTAAAGTTGCTACGAGTAGTAAAGATACAAGAATCTGCACGGAAATTGAACAGAGGGTCTTGGAAGAGGAAGAAAACCTTGATGTTAACAGCAAGACTGTTGTACAATATATCCTCTTTGACAAAAAGGTAGGATTTCCTAGCTTGGAGAGATTGAGCATCGAAGGGTTAAGAGAGTTGAAGGCAATATGGCATGTCCAACTTGCTCGAGACTCTTTTCGGAGACTCAAGCAAGTAGATGTCCAGGAATGCAAAAATCTAATAAGTTTGTTTCCGCCTAGTGTCATGGGAAGATTGAATGCCCTGGAAACTTTGAAGATATGGTGCTGCGGATCACTAGAAGTGATATTTGAATTACGCGGAAGAAGTAACGTTAAAGAAATACAGGTCGACACATCATTCCCTCATGACCAATTGAAACATTTTGATTGTCAAAATCTTGATTCAATAAACATCTACAACTGTCATAGTTTGAAACAGATTTTCCCAGCCTCAGTGGCCAGAGGTCTTCAAAACCTTACTCAGTTGGATGTGGACAAATGTGATGCAATTGAGGAAATTGTTGGTGAGGAAGAGGAAAAAGAAGGTCTAGAAACAATACCTCCTCATTTTGTGTTCCCAAAAGTAACATCAGTGAAGTTGAAGAATCTGCCTCAACTTACGAGTTTCTATCCCAAAACACATGCTTCCCAGTGTCCATTACTTGTACAATTGAAAGTGGTGAGATGTGATAAAGTGAAAATCTTTGCCGCAGAATTTTCATTCTCTCAAAAGAAACATGAGACGAATCATCTAATGTCACAATCACCTTTATTTTCAATCAACGAGGATTCGTTCCTCGACTTGGAAGACTTACAATTGAATGACATGGAAATTTGGAATGGACCAACCCCGGAAAATCCGTTGGTGTTTGGTAAATTAAAATCAATTACCTTTCGTGGGAGTCGTGCACCTTTCTCCAAGCAAGCTTCAGTCATTTTCCTCCAGAGATTACAAAATCTTGAAGAAATTGAAATTGACAATTGTTCTTGGGAAGCAATATTTGTCAATCAAGGAATTAGTACTGGGGAAGAAAAACATGATGATGGAGTTAGGAGTGGGGGCCTCCCGCGTGTAAGGATTTTGAGCCTCGAGAGTATGGACGAACTGATGCATCTAGGGAATGACAACTCCCAATCACTACTTTTTCCAAATTTGGAAACTCTTAAGGTGTCGCTCTGTACCAGATTAAAGAGTCTGGAGTCATATGCAATATTCTTCCGAAATCTAACGACTTTGACAATATTTGCTTGTGATGGATTGGGATATTTAACAAGTTATTCGGTGGCTAAAAACTTAGTGCAACTCACGACTTTGGAAGTGGACTACTGTGATGGAATTGTAGTAATAGTGTCAAGCAACGGAGATGATGATGCTGATGTAGCAAGAAATGAGATTTCTTTCAGCCGCTTGCAGCATTTGGAGCTTACTCGTCTACCAAGTCTACAAGGCTTTTGCTCCGGGAATTGCACACTCAAATTCCCATCCTCCATGACCTTACAAGTAGAAGACTGCCCGATCAAGTTGAAGATTTCCCCTGAAGGAGTTCTCGTGACAGAGATAGACCATCAATTTCAGCAAAACAGAGTTGACTATGATAGTGATGATGATGATGAGGAGGAGGATGTTGAAAAGGCTGAGAGAAATCCTGAAATATTATCAGAAGGGCCTGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

943

Amino Acids

106.96

Weight (kDa)

4.9

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 248 - 376 3.1e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 415 - 482 5.6e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 422 - 499 4.9e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 507 - 618 7.6e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 658 - 784 1.3e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 789 - 890 4.2e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000109)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11460 FvH4_1g20081 FvH4_1g21331 FvH4_1g22022 FvH4_1g27932 FvH4_2g04010 FvH4_2g07601 FvH4_2g09181 FvH4_2g18601 FvH4_2g24951 FvH4_2g27371 FvH4_3g15420 FvH4_3g26790 FvH4_3g26791 FvH4_3g34441 FvH4_3g34930 FvH4_3g45133 FvH4_4g02561 FvH4_4g05731 FvH4_4g05732 FvH4_4g06570 FvH4_4g09962 FvH4_4g13551 FvH4_4g13621 FvH4_4g14830 FvH4_4g15081 FvH4_4g26661 FvH4_5g05871 FvH4_5g08822 FvH4_5g12461 FvH4_5g24922 FvH4_5g30051 FvH4_5g30200 FvH4_5g30551 FvH4_5g32991 FvH4_5g36132 FvH4_5g37291 FvH4_6g07931 FvH4_6g08641 FvH4_6g12800 FvH4_6g13840 FvH4_6g13900 FvH4_6g16432 FvH4_6g16701 FvH4_6g28831 FvH4_6g31171 FvH4_6g41261 FvH4_7g00030 FvH4_7g01201 FvH4_7g08120 FvH4_7g10071 FvH4_7g18780
rosa_chinensis RchiOBHm_Chr2g0119561 RchiOBHm_Chr3g0458511 RchiOBHm_Chr3g0477731 RchiOBHm_Chr3g0479111 RchiOBHm_Chr3g0497091 RchiOBHm_Chr5g0072451 RchiOBHm_Chr6g0284681 RchiOBHm_Chr6g0311691 RchiOBHm_Chr7g0182191
rosa_laevigata RLG00000000983 RLG00000001598 RLG00000004616 RLG00000004931 RLG00000006806 RLG00000008371 RLG00000009544 RLG00000009718 RLG00000013465 RLG00000013533 RLG00000014631 RLG00000014987 RLG00000015588 RLG00000016226 RLG00000018171 RLG00000018433 RLG00000018649 RLG00000018710 RLG00000018870 RLG00000019205 RLG00000020638 RLG00000023424 RLG00000024707 RLG00000025967 RLG00000030020 RLG00000030596 RLG00000030996 RLG00000033451 RLG00000033757 RLG00000033758 RLG00000033808 RLG00000033812 RLG00000034857 RLG00000035240 RLG00000035691 RLG00000036232 RLG00000036301 RLG00000036412
rosa_multiflora Rmu_co8113544.1_g000001 Rmu_co8372331.1_g000001 Rmu_sc0000298.1_g000003 Rmu_sc0000552.1_g000026 Rmu_sc0000814.1_g000037 Rmu_sc0001211.1_g000126 Rmu_sc0001311.1_g000026 Rmu_sc0001354.1_g000009 Rmu_sc0002119.1_g000029 Rmu_sc0002357.1_g000020 Rmu_sc0002573.1_g000014 Rmu_sc0002586.1_g000022 Rmu_sc0003517.1_g000004 Rmu_sc0003558.1_g000001 Rmu_sc0004084.1_g000044 Rmu_sc0004143.1_g000011 Rmu_sc0004514.1_g000002 Rmu_sc0005370.1_g000004 Rmu_sc0005392.1_g000015 Rmu_sc0005664.1_g000009 Rmu_sc0006235.1_g000009 Rmu_sc0006423.1_g000001 Rmu_sc0006838.1_g000018 Rmu_sc0006847.1_g000005 Rmu_sc0006959.1_g000019 Rmu_sc0007111.1_g000002 Rmu_sc0009125.1_g000001 Rmu_sc0009546.1_g000015 Rmu_sc0009670.1_g000006 Rmu_sc0011618.1_g000004 Rmu_sc0012306.1_g000002 Rmu_sc0012306.1_g000003 Rmu_sc0012355.1_g000001 Rmu_sc0012355.1_g000002 Rmu_sc0013116.1_g000002 Rmu_sc0016872.1_g000001 Rmu_sc0017043.1_g000005 Rmu_sc0017081.1_g000003 Rmu_sc0018075.1_g000002 Rmu_sc0020578.1_g000002 Rmu_sc0034515.1_g000001 Rmu_sc0040072.1_g000003 Rmu_ssc0000309.1_g000056 Rmu_ssc0000330.1_g000048
rosa_roxburghii Rroxscaffold_1G00007410 Rroxscaffold_1G00027350 Rroxscaffold_1G00043810 Rroxscaffold_2G00099880 Rroxscaffold_2G00112650 Rroxscaffold_2G00122020 Rroxscaffold_3G00223350 Rroxscaffold_3G00239670 Rroxscaffold_5G00333740 Rroxscaffold_5G00335340 Rroxscaffold_5G00341030 Rroxscaffold_5G00342730 Rroxscaffold_6G00426030 Rroxscaffold_7G00162470 Rroxscaffold_7G00196110 Rroxscaffold_7G00202440 Rroxscaffold_7G00202450 Rroxscaffold_7G00215740
rosa_rugosa Rorug01G0176600 Rorug01G0276600 Rorug02G0236500 Rorug02G0277000 Rorug02G0363300 Rorug02G0375900 Rorug02G0473700 Rorug02G0546200 Rorug02G0601000 Rorug03G0158400 Rorug03G0289500 Rorug04G0026600 Rorug04G0090000 Rorug04G0105500 Rorug04G0263100 Rorug04G0394500 Rorug05G0122200 Rorug05G0152200 Rorug05G0541800 Rorug05G0551100 Rorug05G0554400 Rorug06G0244900 Rorug06G0401400
rosa_samantha Rh1CG158000 Rh1CG280800 Rh1CG326900 Rh2BG051500 Rh2BG101500 Rh2BG438200 Rh2BG509100 Rh2BG531600 Rh2BG531800 Rh2BG629800 Rh2BG629900 Rh2CG565300 Rh2DG448000 Rh2DG540600 Rh2DG540700 Rh2DG641700 Rh2DG641800 Rh3AG222200 Rh3BG034200 Rh3BG254700 Rh3DG286800 Rh3DG322600 Rh4AG009200 Rh4AG050400 Rh4AG223100 Rh4BG072500 Rh4BG106000 Rh4CG010200 Rh4CG053700 Rh4CG236100 Rh4CG236200 Rh4CG236300 Rh4DG104800 Rh5AG474800 Rh5BG214500 Rh5BG360000 Rh5BG403700 Rh5BG494900 Rh5DG255300 Rh6AG205400 Rh6BG227300 Rh6CG280700 Rh6DG273400 Rh7AG187300 Rh7AG220800 Rh7AG355700 Rh7AG462000 Rh7AG462200 Rh7AG465900 Rh7CG198300 Rh7CG222100 Rh7CG373000 Rh7CG479300 Rh7DG233800
rosa_wichuraiana Rw0G018350 Rw1G026500 Rw2G024750 Rw2G051200 Rw5G001570 Rw5G036850 Rw6G016070 Rw6G042710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 1503, 2587, 2596
AccII CGCG 2 cut(s) 1473, 2224
AciI CCGC 7 cut(s) 450, 1388, 1443, 1473, 1863, 2222, 2560
AclI AACGTT 1 cut(s) 1485
AclWI GGATC 1 cut(s) 1453
AcoI YGGCCR 1 cut(s) 1611
AcsI RAATTY 7 cut(s) 210, 1052, 1103, 1868, 1986, 2305, 2630
AcuI CTGAAG 4 cut(s) 680, 978, 2067, 2712
AfaI GTAC 4 cut(s) 1218, 1821, 2176, 2335
AfiI CCNNNNNNNGG 3 cut(s) 715, 1957, 2052
AflII CTTAAG 1 cut(s) 2318
AflIII ACRYGT 2 cut(s) 93, 911
AjnI CCWGG 2 cut(s) 1356, 1416
Alw21I GWGCWC 1 cut(s) 2065
Alw26I GTCTC 4 cut(s) 444, 1317, 1330, 1889
Alw44I GTGCAC 1 cut(s) 2061
AlwI GGATC 1 cut(s) 1453
Ama87I CYCGRG 3 cut(s) 188, 1320, 2243
AoxI GGCC 5 cut(s) 643, 1059, 1611, 2215, 2819
ApaLI GTGCAC 1 cut(s) 2061
ApeKI GCWGC 4 cut(s) 193, 578, 1440, 2566
ApoI RAATTY 7 cut(s) 210, 1052, 1103, 1868, 1986, 2305, 2630
AseI ATTAAT 2 cut(s) 699, 786
Asp700I GAANNNNTTC 2 cut(s) 1011, 2697
AspLEI GCGC 2 cut(s) 956, 1115
AspS9I GGNCC 3 cut(s) 1997, 2215, 2819
AsuC2I CCSGG 2 cut(s) 2006, 2614
AsuHPI GGTGA 6 cut(s) 312, 355, 410, 1691, 1846, 1911
AsuNHI GCTAGC 1 cut(s) 2825
AvaI CYCGRG 3 cut(s) 188, 1320, 2243
AvaII GGWCC 1 cut(s) 1997
BaeGI GKGCMC 1 cut(s) 2065
BalI TGGCCA 1 cut(s) 1613
BauI CACGAG 1 cut(s) 2702
BbsI GAAGAC 5 cut(s) 140, 266, 1613, 1968, 2667
Bbv12I GWGCWC 1 cut(s) 2065
BbvI GCAGC 4 cut(s) 205, 565, 1427, 2578
BccI CCATC 7 cut(s) 49, 112, 2192, 2402, 2480, 2644, 2727
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 2 cut(s) 1358, 1418
BciVI GTATCC 1 cut(s) 712
BcnI CCSGG 2 cut(s) 2006, 2614
BcoDI GTCTC 4 cut(s) 444, 1317, 1330, 1889
BfmI CTRYAG 1 cut(s) 579
BfoI RGCGCY 1 cut(s) 1116
BfrI CTTAAG 1 cut(s) 2318
BfuI GTATCC 1 cut(s) 712
BglI GCCNNNNNGGC 1 cut(s) 1610
BglII AGATCT 2 cut(s) 157, 665
BisI GCNGC 6 cut(s) 194, 579, 1441, 1863, 2560, 2567
BlsI GCNGC 6 cut(s) 195, 580, 1442, 1864, 2561, 2568
BmcAI AGTACT 1 cut(s) 2176
Bme1390I CCNGG 4 cut(s) 1358, 1418, 2006, 2614
Bme18I GGWCC 1 cut(s) 1997
BmeT110I CYCGRG 3 cut(s) 188, 1320, 2243
BmgT120I GGNCC 3 cut(s) 1997, 2215, 2819
BmiI GGNNCC 1 cut(s) 2216
BmrFI CCNGG 4 cut(s) 1358, 1418, 2006, 2614
BmrI ACTGGG 2 cut(s) 1798, 2187
BmsI GCATC 8 cut(s) 51, 529, 538, 1281, 1648, 2253, 2275, 2515
BmtI GCTAGC 1 cut(s) 2829
BmuI ACTGGG 2 cut(s) 1798, 2187
BpiI GAAGAC 5 cut(s) 140, 266, 1613, 1968, 2667
BpmI CTGGAG 2 cut(s) 2081, 2372
BpuEI CTTGAG 3 cut(s) 56, 590, 1325
BpuMI CCSGG 2 cut(s) 2006, 2614
BsaJI CCNNGG 5 cut(s) 681, 819, 1062, 1416, 2004
BsaXI ACNNNNNCTCC 6 cut(s) 485, 515, 1699, 1729, 2688, 2718
Bsc4I CCNNNNNNNGG 3 cut(s) 715, 1957, 2052
Bse1I ACTGG 2 cut(s) 1804, 2182
Bse3DI GCAATG 1 cut(s) 107
BseBI CCWGG 2 cut(s) 1358, 1418
BseDI CCNNGG 5 cut(s) 681, 819, 1062, 1416, 2004
BseGI GGATG 7 cut(s) 553, 571, 691, 839, 1648, 2636, 2782
BseLI CCNNNNNNNGG 3 cut(s) 715, 1957, 2052
BseMI GCAATG 1 cut(s) 107
BseMII CTCAG 7 cut(s) 285, 414, 455, 660, 1620, 1649, 2781
BseNI ACTGG 2 cut(s) 1804, 2182
BseRI GAGGAG 3 cut(s) 1707, 2783, 2786
BseSI GKGCMC 1 cut(s) 2065
BseXI GCAGC 4 cut(s) 205, 565, 1427, 2578
BseYI CCCAGC 1 cut(s) 1600
BsgI GTGCAG 1 cut(s) 1135
Bsh1236I CGCG 2 cut(s) 1473, 2224
BshFI GGCC 5 cut(s) 645, 1061, 1613, 2217, 2821
BsiHKAI GWGCWC 1 cut(s) 2065
BsiHKCI CYCGRG 3 cut(s) 188, 1320, 2243
BsiSI CCGG 4 cut(s) 709, 1058, 2006, 2613
BslFI GGGAC 1 cut(s) 23
BslI CCNNNNNNNGG 3 cut(s) 715, 1957, 2052
BsmAI GTCTC 4 cut(s) 444, 1317, 1330, 1889
BsmBI CGTCTC 1 cut(s) 1889
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 4 cut(s) 478, 956, 1367, 1417
BsnI GGCC 5 cut(s) 645, 1061, 1613, 2217, 2821
BsoBI CYCGRG 3 cut(s) 188, 1320, 2243
Bsp1286I GDGCHC 1 cut(s) 2065
Bsp1407I TGTACA 2 cut(s) 1216, 1819
Bsp143I GATC 4 cut(s) 157, 665, 1445, 2670
BspACI CCGC 7 cut(s) 450, 1388, 1443, 1473, 1863, 2222, 2560
BspANI GGCC 5 cut(s) 645, 1061, 1613, 2217, 2821
BspCNI CTCAG 7 cut(s) 286, 415, 454, 661, 1619, 1648, 2782
BspFNI CGCG 2 cut(s) 1473, 2224
BspHI TCATGA 1 cut(s) 1519
BspLI GGNNCC 1 cut(s) 2216
BspMAI CTGCAG 1 cut(s) 583
BspOI GCTAGC 1 cut(s) 2829
BspPI GGATC 1 cut(s) 1453
BspTI CTTAAG 1 cut(s) 2318
BsrDI GCAATG 1 cut(s) 107
BsrGI TGTACA 2 cut(s) 1216, 1819
BsrI ACTGG 2 cut(s) 1804, 2182
BssECI CCNNGG 5 cut(s) 681, 819, 1062, 1416, 2004
BssMI GATC 4 cut(s) 157, 665, 1445, 2670
BssSI CACGAG 1 cut(s) 2702
BssT1I CCWWGG 3 cut(s) 681, 819, 1062
Bst2BI CACGAG 1 cut(s) 2702
Bst2UI CCWGG 2 cut(s) 1358, 1418
Bst4CI ACNGT 5 cut(s) 17, 328, 1213, 1577, 2483
Bst6I CTCTTC 4 cut(s) 126, 656, 1173, 1680
BstAFI CTTAAG 1 cut(s) 2318
BstAUI TGTACA 2 cut(s) 1216, 1819
BstC8I GCNNGC 5 cut(s) 415, 2079, 2564, 2823, 2827
BstF5I GGATG 7 cut(s) 553, 571, 691, 839, 1648, 2636, 2782
BstFNI CGCG 2 cut(s) 1473, 2224
BstH2I RGCGCY 1 cut(s) 1116
BstHHI GCGC 2 cut(s) 956, 1115
BstKTI GATC 4 cut(s) 160, 668, 1448, 2673
BstMAI GTCTC 4 cut(s) 444, 1317, 1330, 1889
BstMBI GATC 4 cut(s) 157, 665, 1445, 2670
BstMWI GCNNNNNNNGC 6 cut(s) 109, 548, 1121, 1610, 2534, 2575
BstNI CCWGG 2 cut(s) 1358, 1418
BstNSI RCATGY 4 cut(s) 97, 915, 1310, 1799
BstSCI CCNGG 4 cut(s) 1356, 1416, 2004, 2612
BstSFI CTRYAG 1 cut(s) 579
BstSLI GKGCMC 1 cut(s) 2065
BstUI CGCG 2 cut(s) 1473, 2224
BstV1I GCAGC 4 cut(s) 205, 565, 1427, 2578
BstV2I GAAGAC 5 cut(s) 140, 266, 1613, 1968, 2667
BstX2I RGATCY 2 cut(s) 157, 665
BstYI RGATCY 2 cut(s) 157, 665
BsuI GTATCC 1 cut(s) 712
BsuRI GGCC 5 cut(s) 645, 1061, 1613, 2217, 2821
BtsCI GGATG 7 cut(s) 553, 571, 691, 839, 1648, 2636, 2782
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 4 cut(s) 588, 1614, 1752, 1811
Cac8I GCNNGC 5 cut(s) 415, 2079, 2564, 2823, 2827
CciI TCATGA 1 cut(s) 1519
CfoI GCGC 2 cut(s) 956, 1115
Cfr13I GGNCC 3 cut(s) 1997, 2215, 2819
CseI GACGC 1 cut(s) 145
Csp6I GTAC 4 cut(s) 1217, 1820, 2175, 2334
CviQI GTAC 4 cut(s) 1217, 1820, 2175, 2334
DpnI GATC 4 cut(s) 159, 667, 1447, 2672
DpnII GATC 4 cut(s) 157, 665, 1445, 2670
DraI TTTAAA 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 1611
Eam1104I CTCTTC 4 cut(s) 126, 656, 1173, 1680
EarI CTCTTC 4 cut(s) 126, 656, 1173, 1680
EciI GGCGGA 1 cut(s) 1377
Eco130I CCWWGG 3 cut(s) 681, 819, 1062
Eco147I AGGCCT 1 cut(s) 645
Eco47I GGWCC 1 cut(s) 1997
Eco57I CTGAAG 4 cut(s) 680, 978, 2067, 2712
Eco88I CYCGRG 3 cut(s) 188, 1320, 2243
EcoO109I RGGNCCY 2 cut(s) 2215, 2819
EcoRI GAATTC 1 cut(s) 1103
EcoRII CCWGG 2 cut(s) 1356, 1416
EcoT14I CCWWGG 3 cut(s) 681, 819, 1062
EcoT22I ATGCAT 1 cut(s) 2268
ErhI CCWWGG 3 cut(s) 681, 819, 1062
Esp3I CGTCTC 1 cut(s) 1889
FalI AAGNNNNNCTT 2 cut(s) 1176, 1208
FaqI GGGAC 1 cut(s) 23
FauI CCCGC 1 cut(s) 2229
FauNDI CATATG 1 cut(s) 2359
FblI GTMKAC 3 cut(s) 1503, 2587, 2596
Fnu4HI GCNGC 6 cut(s) 194, 579, 1441, 1863, 2560, 2567
FokI GGATG 7 cut(s) 560, 578, 698, 846, 1655, 2623, 2789
Fsp4HI GCNGC 6 cut(s) 194, 579, 1441, 1863, 2560, 2567
GlaI GCGC 2 cut(s) 955, 1114
GluI GCNGC 6 cut(s) 194, 579, 1441, 1863, 2560, 2567
GsaI CCCAGC 1 cut(s) 1604
GsuI CTGGAG 2 cut(s) 2081, 2372
HaeII RGCGCY 1 cut(s) 1116
HaeIII GGCC 5 cut(s) 645, 1061, 1613, 2217, 2821
HapII CCGG 4 cut(s) 709, 1058, 2006, 2613
HgaI GACGC 1 cut(s) 145
HhaI GCGC 2 cut(s) 956, 1115
Hin6I GCGC 2 cut(s) 954, 1113
HinP1I GCGC 2 cut(s) 954, 1113
HincII GTYRAC 3 cut(s) 1201, 1504, 2743
HindII GTYRAC 3 cut(s) 1201, 1504, 2743
HindIII AAGCTT 2 cut(s) 337, 2079
HpaI GTTAAC 1 cut(s) 1201
HpaII CCGG 4 cut(s) 709, 1058, 2006, 2613
HphI GGTGA 6 cut(s) 312, 355, 410, 1691, 1846, 1911
Hpy188I TCNGA 9 cut(s) 232, 295, 424, 471, 670, 752, 1335, 2375, 2815
HpyAV CCTTC 7 cut(s) 389, 635, 1271, 1288, 1691, 2687, 2810
HpyCH4III ACNGT 5 cut(s) 17, 328, 1213, 1577, 2483
HpyCH4IV ACGT 1 cut(s) 1485
HpyF10VI GCNNNNNNNGC 6 cut(s) 109, 548, 1121, 1610, 2534, 2575
HpySE526I ACGT 1 cut(s) 1485
HspAI GCGC 2 cut(s) 954, 1113
KspAI GTTAAC 1 cut(s) 1201
Kzo9I GATC 4 cut(s) 157, 665, 1445, 2670
LmnI GCTCC 2 cut(s) 2575, 2615
Lsp1109I GCAGC 4 cut(s) 205, 565, 1427, 2578
LweI GCATC 8 cut(s) 51, 529, 538, 1281, 1648, 2253, 2275, 2515
MaeII ACGT 1 cut(s) 1485
MaeIII GTNAC 6 cut(s) 583, 814, 1481, 1738, 1911, 2704
MalI GATC 4 cut(s) 159, 667, 1447, 2672
MboI GATC 4 cut(s) 157, 665, 1445, 2670
MfeI CAATTG 7 cut(s) 263, 713, 1526, 1662, 1823, 1970, 2134
MflI RGATCY 2 cut(s) 157, 665
MhlI GDGCHC 1 cut(s) 2065
MlsI TGGCCA 1 cut(s) 1613
MluNI TGGCCA 1 cut(s) 1613
MlyI GAGTC 7 cut(s) 262, 747, 1319, 1332, 2065, 2356, 2363
MmeI TCCRAC 4 cut(s) 264, 775, 1336, 1620
Mox20I TGGCCA 1 cut(s) 1613
Mph1103I ATGCAT 1 cut(s) 2268
MroXI GAANNNNTTC 2 cut(s) 1011, 2697
MscI TGGCCA 1 cut(s) 1613
MslI CAYNNNNRTG 3 cut(s) 98, 834, 2196
Msp20I TGGCCA 1 cut(s) 1613
MspCI CTTAAG 1 cut(s) 2318
MspI CCGG 4 cut(s) 709, 1058, 2006, 2613
MspR9I CCNGG 4 cut(s) 1358, 1418, 2006, 2614
MunI CAATTG 7 cut(s) 263, 713, 1526, 1662, 1823, 1970, 2134
Mva1269I GAATGC 4 cut(s) 478, 956, 1367, 1417
MvaI CCWGG 2 cut(s) 1358, 1418
MvnI CGCG 2 cut(s) 1473, 2224
MwoI GCNNNNNNNGC 6 cut(s) 109, 548, 1121, 1610, 2534, 2575
NciI CCSGG 2 cut(s) 2006, 2614
NdeI CATATG 1 cut(s) 2359
NdeII GATC 4 cut(s) 157, 665, 1445, 2670
NheI GCTAGC 1 cut(s) 2825
NlaIV GGNNCC 1 cut(s) 2216
NmuCI GTSAC 3 cut(s) 583, 1911, 2704
NsiI ATGCAT 1 cut(s) 2268
NspI RCATGY 4 cut(s) 97, 915, 1310, 1799
PaeR7I CTCGAG 2 cut(s) 1320, 2243
PagI TCATGA 1 cut(s) 1519
PceI AGGCCT 1 cut(s) 645
PciI ACATGT 2 cut(s) 93, 911
PcsI WCGNNNNNNNCGW 1 cut(s) 2056
PctI GAATGC 4 cut(s) 478, 956, 1367, 1417
PdmI GAANNNNTTC 2 cut(s) 1011, 2697
PfeI GAWTC 6 cut(s) 26, 1146, 1556, 1759, 1900, 1943
PfoI TCCNGGA 2 cut(s) 1356, 2612
PkrI GCNGC 6 cut(s) 195, 580, 1442, 1864, 2561, 2568
PleI GAGTC 7 cut(s) 262, 747, 1319, 1332, 2064, 2355, 2362
PpsI GAGTC 7 cut(s) 262, 747, 1319, 1332, 2064, 2355, 2362
PscI ACATGT 2 cut(s) 93, 911
PshBI ATTAAT 2 cut(s) 699, 786
Psp1406I AACGTT 1 cut(s) 1485
Psp6I CCWGG 2 cut(s) 1356, 1416
PspFI CCCAGC 1 cut(s) 1600
PspGI CCWGG 2 cut(s) 1356, 1416
PspN4I GGNNCC 1 cut(s) 2216
PspPI GGNCC 3 cut(s) 1997, 2215, 2819
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 2 cut(s) 157, 665
RsaI GTAC 4 cut(s) 1218, 1821, 2176, 2335
RsaNI GTAC 4 cut(s) 1217, 1820, 2175, 2334
RseI CAYNNNNRTG 3 cut(s) 98, 834, 2196
SalI GTCGAC 1 cut(s) 1502
SatI GCNGC 6 cut(s) 194, 579, 1441, 1863, 2560, 2567
Sau3AI GATC 4 cut(s) 157, 665, 1445, 2670
Sau96I GGNCC 3 cut(s) 1997, 2215, 2819
ScaI AGTACT 1 cut(s) 2176
SchI GAGTC 7 cut(s) 262, 747, 1319, 1332, 2065, 2356, 2363
ScrFI CCNGG 4 cut(s) 1358, 1418, 2006, 2614
SduI GDGCHC 1 cut(s) 2065
SfaNI GCATC 8 cut(s) 51, 529, 538, 1281, 1648, 2253, 2275, 2515
SfcI CTRYAG 1 cut(s) 579
Sfr274I CTCGAG 2 cut(s) 1320, 2243
SinI GGWCC 1 cut(s) 1997
SlaI CTCGAG 2 cut(s) 1320, 2243
SmiMI CAYNNNNRTG 3 cut(s) 98, 834, 2196
SmlI CTYRAG 6 cut(s) 71, 605, 1320, 1340, 2243, 2318
SmoI CTYRAG 6 cut(s) 71, 605, 1320, 1340, 2243, 2318
SseBI AGGCCT 1 cut(s) 645
SsiI CCGC 7 cut(s) 450, 1388, 1443, 1473, 1863, 2222, 2560
SspI AATATT 9 cut(s) 526, 625, 703, 766, 994, 2154, 2367, 2397, 2808
StuI AGGCCT 1 cut(s) 645
StyD4I CCNGG 4 cut(s) 1356, 1416, 2004, 2612
StyI CCWWGG 3 cut(s) 681, 819, 1062
TaaI ACNGT 5 cut(s) 17, 328, 1213, 1577, 2483
TaiI ACGT 1 cut(s) 1488
TaqI TCGA 6 cut(s) 44, 1275, 1321, 1503, 1953, 2244
TatI WGTACW 3 cut(s) 1216, 1819, 2174
TauI GCSGC 2 cut(s) 1865, 2562
TfiI GAWTC 6 cut(s) 26, 1146, 1556, 1759, 1900, 1943
TscAI CASTG 4 cut(s) 588, 1614, 1752, 1811
TseFI GTSAC 3 cut(s) 583, 1911, 2704
TseI GCWGC 4 cut(s) 193, 578, 1440, 2566
Tsp45I GTSAC 3 cut(s) 583, 1911, 2704
TspDTI ATGAA 4 cut(s) 582, 654, 1096, 1863
TspGWI ACGGA 3 cut(s) 1169, 2004, 2529
TspRI CASTG 4 cut(s) 588, 1614, 1752, 1811
Vha464I CTTAAG 1 cut(s) 2318
VneI GTGCAC 1 cut(s) 2061
VpaK11BI GGWCC 1 cut(s) 1997
VspI ATTAAT 2 cut(s) 699, 786
XapI RAATTY 7 cut(s) 210, 1052, 1103, 1868, 1986, 2305, 2630
XbaI TCTAGA 1 cut(s) 1702
XceI RCATGY 4 cut(s) 97, 915, 1310, 1799
XhoI CTCGAG 2 cut(s) 1320, 2243
XmiI GTMKAC 3 cut(s) 1503, 2587, 2596
XmnI GAANNNNTTC 2 cut(s) 1011, 2697
ZrmI AGTACT 1 cut(s) 2176
Zsp2I ATGCAT 1 cut(s) 2268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.