Rorug05G0551100

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000109
Physical Location & Seq
Forward (+)
73927552 .. 73930347
2796 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0551100.1

Sequence Viewer

Length: 960 bp
ATGGATGCCAACTATTGGAGCCCTTCTCAAGGAGGAGAACAGTCTATGTATGTAGATGATTGGAGGAGCCAATTGCTGCCAGATTTGCGGCAAGTGTTTCTTAATAAGATAATGGATACATTGAAAAGGCATCTGCCTTTCTCTAGTCAAGAGGGTTTACTTGAACTCCAGAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCTGCCACAAGTCAGTCGGATTATGAACGAAAAATTTCTCTGAAGATGCTCACAATGGAGACCAAGTCGCAGAACACAAGGGGCAGCTCTATACAATTCAACTCAGCCAGCAATAGCAAGAGACTCCCCGATCCTGTTTTAATGAATACCAGTAACAATGGGAGGCCTCCTCAAGGTGGAGGACCATCTATGGACACATCAGATTGGAGTCAATTGCCGTCAGATGCACAACATGGATGGCAACAGGAAGCTCCCCCACAGCAACGGCAGCAAACCTCGAATTCTCAGCAGTACTATATTTACTCGCAGCAGTTTATGTACCAGCAACAGTTACAACAACAACTCCTCCTTCAAAGTCATATTCAACTGCAACAAGAGCAGCAGCAGATTCCACCACAAAAAACTCAGTTGCAATCTTCTCAGCAATCAGCATCTGTTATGCAACCTTCGGTGGAGCCATTGCATCTCTCTGTGCAACCTTCTGAGCAATTGCCGCCAGATGCGCGACATAGAATTGTCAACAAAATAACTAATGTGTTAAAGAGGAATCCGTCCTGTGGTCAAATGGGATTACCAGAACTCGAGAGAATCGCAGCAACACTTGAGGAGAAGATATATGCCGTTGCCTCAAGCCAGGCGGATTATCTGCGTAAAGTAAGTCTGAAGATGCTCACCATGGAGACGCAAATCACAGCTCCCCCATCCAATGACAGCTTGGAATCATTATTTGCTTCCCTGGAGTTGCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

319

Amino Acids

36.29

Weight (kDa)

5.8

Isoelectric Point (pI)

74.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 98 9.7e-31 KIX domain
KIX_2 PF16987 231 - 304 7.2e-22 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000109)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g11460 FvH4_1g20081 FvH4_1g21331 FvH4_1g22022 FvH4_1g27932 FvH4_2g04010 FvH4_2g07601 FvH4_2g09181 FvH4_2g18601 FvH4_2g24951 FvH4_2g27371 FvH4_3g15420 FvH4_3g26790 FvH4_3g26791 FvH4_3g34441 FvH4_3g34930 FvH4_3g45133 FvH4_4g02561 FvH4_4g05731 FvH4_4g05732 FvH4_4g06570 FvH4_4g09962 FvH4_4g13551 FvH4_4g13621 FvH4_4g14830 FvH4_4g15081 FvH4_4g26661 FvH4_5g05871 FvH4_5g08822 FvH4_5g12461 FvH4_5g24922 FvH4_5g30051 FvH4_5g30200 FvH4_5g30551 FvH4_5g32991 FvH4_5g36132 FvH4_5g37291 FvH4_6g07931 FvH4_6g08641 FvH4_6g12800 FvH4_6g13840 FvH4_6g13900 FvH4_6g16432 FvH4_6g16701 FvH4_6g28831 FvH4_6g31171 FvH4_6g41261 FvH4_7g00030 FvH4_7g01201 FvH4_7g08120 FvH4_7g10071 FvH4_7g18780
rosa_chinensis RchiOBHm_Chr2g0119561 RchiOBHm_Chr3g0458511 RchiOBHm_Chr3g0477731 RchiOBHm_Chr3g0479111 RchiOBHm_Chr3g0497091 RchiOBHm_Chr5g0072451 RchiOBHm_Chr6g0284681 RchiOBHm_Chr6g0311691 RchiOBHm_Chr7g0182191
rosa_laevigata RLG00000000983 RLG00000001598 RLG00000004616 RLG00000004931 RLG00000006806 RLG00000008371 RLG00000009544 RLG00000009718 RLG00000013465 RLG00000013533 RLG00000014631 RLG00000014987 RLG00000015588 RLG00000016226 RLG00000018171 RLG00000018433 RLG00000018649 RLG00000018710 RLG00000018870 RLG00000019205 RLG00000020638 RLG00000023424 RLG00000024707 RLG00000025967 RLG00000030020 RLG00000030596 RLG00000030996 RLG00000033451 RLG00000033757 RLG00000033758 RLG00000033808 RLG00000033812 RLG00000034857 RLG00000035240 RLG00000035691 RLG00000036232 RLG00000036301 RLG00000036412
rosa_multiflora Rmu_co8113544.1_g000001 Rmu_co8372331.1_g000001 Rmu_sc0000298.1_g000003 Rmu_sc0000552.1_g000026 Rmu_sc0000814.1_g000037 Rmu_sc0001211.1_g000126 Rmu_sc0001311.1_g000026 Rmu_sc0001354.1_g000009 Rmu_sc0002119.1_g000029 Rmu_sc0002357.1_g000020 Rmu_sc0002573.1_g000014 Rmu_sc0002586.1_g000022 Rmu_sc0003517.1_g000004 Rmu_sc0003558.1_g000001 Rmu_sc0004084.1_g000044 Rmu_sc0004143.1_g000011 Rmu_sc0004514.1_g000002 Rmu_sc0005370.1_g000004 Rmu_sc0005392.1_g000015 Rmu_sc0005664.1_g000009 Rmu_sc0006235.1_g000009 Rmu_sc0006423.1_g000001 Rmu_sc0006838.1_g000018 Rmu_sc0006847.1_g000005 Rmu_sc0006959.1_g000019 Rmu_sc0007111.1_g000002 Rmu_sc0009125.1_g000001 Rmu_sc0009546.1_g000015 Rmu_sc0009670.1_g000006 Rmu_sc0011618.1_g000004 Rmu_sc0012306.1_g000002 Rmu_sc0012306.1_g000003 Rmu_sc0012355.1_g000001 Rmu_sc0012355.1_g000002 Rmu_sc0013116.1_g000002 Rmu_sc0016872.1_g000001 Rmu_sc0017043.1_g000005 Rmu_sc0017081.1_g000003 Rmu_sc0018075.1_g000002 Rmu_sc0020578.1_g000002 Rmu_sc0034515.1_g000001 Rmu_sc0040072.1_g000003 Rmu_ssc0000309.1_g000056 Rmu_ssc0000330.1_g000048
rosa_roxburghii Rroxscaffold_1G00007410 Rroxscaffold_1G00027350 Rroxscaffold_1G00043810 Rroxscaffold_2G00099880 Rroxscaffold_2G00112650 Rroxscaffold_2G00122020 Rroxscaffold_3G00223350 Rroxscaffold_3G00239670 Rroxscaffold_5G00333740 Rroxscaffold_5G00335340 Rroxscaffold_5G00341030 Rroxscaffold_5G00342730 Rroxscaffold_6G00426030 Rroxscaffold_7G00162470 Rroxscaffold_7G00196110 Rroxscaffold_7G00202440 Rroxscaffold_7G00202450 Rroxscaffold_7G00215740
rosa_rugosa Rorug01G0176600 Rorug01G0276600 Rorug02G0236500 Rorug02G0277000 Rorug02G0363300 Rorug02G0375900 Rorug02G0473700 Rorug02G0546200 Rorug02G0601000 Rorug03G0158400 Rorug03G0289500 Rorug04G0026600 Rorug04G0090000 Rorug04G0105500 Rorug04G0263100 Rorug04G0394500 Rorug05G0122200 Rorug05G0152200 Rorug05G0541800 Rorug05G0551100 Rorug05G0554400 Rorug06G0244900 Rorug06G0401400
rosa_samantha Rh1CG158000 Rh1CG280800 Rh1CG326900 Rh2BG051500 Rh2BG101500 Rh2BG438200 Rh2BG509100 Rh2BG531600 Rh2BG531800 Rh2BG629800 Rh2BG629900 Rh2CG565300 Rh2DG448000 Rh2DG540600 Rh2DG540700 Rh2DG641700 Rh2DG641800 Rh3AG222200 Rh3BG034200 Rh3BG254700 Rh3DG286800 Rh3DG322600 Rh4AG009200 Rh4AG050400 Rh4AG223100 Rh4BG072500 Rh4BG106000 Rh4CG010200 Rh4CG053700 Rh4CG236100 Rh4CG236200 Rh4CG236300 Rh4DG104800 Rh5AG474800 Rh5BG214500 Rh5BG360000 Rh5BG403700 Rh5BG494900 Rh5DG255300 Rh6AG205400 Rh6BG227300 Rh6CG280700 Rh6DG273400 Rh7AG187300 Rh7AG220800 Rh7AG355700 Rh7AG462000 Rh7AG462200 Rh7AG465900 Rh7CG198300 Rh7CG222100 Rh7CG373000 Rh7CG479300 Rh7DG233800
rosa_wichuraiana Rw0G018350 Rw1G026500 Rw2G024750 Rw2G051200 Rw5G001570 Rw5G036850 Rw6G016070 Rw6G042710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 15
AccII CGCG 1 cut(s) 712
AciI CCGC 3 cut(s) 88, 701, 845
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 2 cut(s) 240, 487
AcuI CTGAAG 2 cut(s) 269, 890
AfaI GTAC 2 cut(s) 500, 527
AfiI CCNNNNNNNGG 5 cut(s) 15, 29, 380, 383, 764
AgsI TTSAA 5 cut(s) 124, 164, 307, 560, 572
AjnI CCWGG 2 cut(s) 840, 942
AluBI AGCT 4 cut(s) 294, 458, 902, 921
AluI AGCT 4 cut(s) 294, 458, 902, 921
Alw26I GTCTC 3 cut(s) 260, 322, 881
AlwI GGATC 1 cut(s) 332
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 788
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 8 cut(s) 76, 209, 291, 475, 514, 586, 589, 800
ApoI RAATTY 2 cut(s) 240, 487
Asp700I GAANNNNTTC 1 cut(s) 241
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 1 cut(s) 871
AvaI CYCGRG 1 cut(s) 788
AvaII GGWCC 1 cut(s) 389
BanII GRGCYC 1 cut(s) 23
BbvI GCAGC 8 cut(s) 63, 196, 303, 487, 526, 598, 601, 812
BccI CCATC 3 cut(s) 400, 438, 916
BceAI ACGGC 3 cut(s) 409, 488, 812
BciT130I CCWGG 2 cut(s) 842, 944
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 3 cut(s) 260, 322, 881
BfaI CTAG 2 cut(s) 144, 958
BfuI GTATCC 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 500
Bme1390I CCNGG 2 cut(s) 842, 944
Bme18I GGWCC 1 cut(s) 389
BmeT110I CYCGRG 1 cut(s) 788
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 3 cut(s) 20, 68, 663
BmrFI CCNGG 2 cut(s) 842, 944
BmsI GCATC 7 cut(s) 139, 243, 421, 647, 679, 697, 864
BplI GAGNNNNNCTC 4 cut(s) 10, 42, 361, 393
BpmI CTGGAG 1 cut(s) 152
BpuEI CTTGAG 4 cut(s) 12, 363, 820, 830
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 882, 942
BsaXI ACNNNNNCTCC 8 cut(s) 150, 180, 257, 287, 534, 537, 564, 567
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 29, 380, 383, 764
Bse1I ACTGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 665
BseBI CCWGG 2 cut(s) 842, 944
BseDI CCNNGG 2 cut(s) 882, 942
BseGI GGATG 3 cut(s) 10, 449, 908
BseLI CCNNNNNNNGG 5 cut(s) 15, 29, 380, 383, 764
BseMI GCAATG 1 cut(s) 665
BseMII CTCAG 5 cut(s) 324, 506, 626, 641, 681
BseNI ACTGG 1 cut(s) 357
BseRI GAGGAG 5 cut(s) 48, 79, 366, 542, 827
BseXI GCAGC 8 cut(s) 63, 196, 303, 487, 526, 598, 601, 812
Bsh1236I CGCG 1 cut(s) 712
BshFI GGCC 1 cut(s) 373
BsiHKCI CYCGRG 1 cut(s) 788
BslI CCNNNNNNNGG 5 cut(s) 15, 29, 380, 383, 764
BsmAI GTCTC 3 cut(s) 260, 322, 881
BsmBI CGTCTC 1 cut(s) 881
BsnI GGCC 1 cut(s) 373
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 788
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 1 cut(s) 337
Bsp19I CCATGG 1 cut(s) 882
BspACI CCGC 3 cut(s) 88, 701, 845
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 5 cut(s) 323, 505, 625, 640, 682
BspFNI CGCG 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 20, 68, 663
BspPI GGATC 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 665
BsrI ACTGG 1 cut(s) 357
BssECI CCNNGG 2 cut(s) 882, 942
BssMI GATC 1 cut(s) 337
BssT1I CCWWGG 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 842, 944
Bst4CI ACNGT 2 cut(s) 42, 537
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 5 cut(s) 310, 492, 612, 627, 690
BstDSI CCRYGG 1 cut(s) 882
BstENI CCTNNNNNAGG 2 cut(s) 27, 378
BstF5I GGATG 3 cut(s) 10, 449, 908
BstFNI CGCG 1 cut(s) 712
BstHHI GCGC 1 cut(s) 712
BstKTI GATC 1 cut(s) 340
BstMAI GTCTC 3 cut(s) 260, 322, 881
BstMBI GATC 1 cut(s) 337
BstMWI GCNNNNNNNGC 5 cut(s) 85, 475, 583, 700, 709
BstNI CCWGG 2 cut(s) 842, 944
BstSCI CCNGG 2 cut(s) 840, 942
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 8 cut(s) 63, 196, 303, 487, 526, 598, 601, 812
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 1 cut(s) 373
BtgI CCRYGG 1 cut(s) 882
BtsCI GGATG 3 cut(s) 10, 449, 908
Cac8I GCNNGC 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 389
CseI GACGC 1 cut(s) 898
Csp6I GTAC 2 cut(s) 499, 526
CviAII CATG 2 cut(s) 440, 883
CviQI GTAC 2 cut(s) 499, 526
DdeI CTNAG 5 cut(s) 310, 492, 612, 627, 690
DpnI GATC 1 cut(s) 339
DpnII GATC 1 cut(s) 337
EciI GGCGGA 1 cut(s) 860
Eco130I CCWWGG 1 cut(s) 882
Eco147I AGGCCT 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 23
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 2 cut(s) 269, 890
Eco88I CYCGRG 1 cut(s) 788
EcoNI CCTNNNNNAGG 2 cut(s) 27, 378
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 840, 942
EcoT14I CCWWGG 1 cut(s) 882
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 882
Esp3I CGTCTC 1 cut(s) 881
FaeI CATG 2 cut(s) 443, 886
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FatI CATG 2 cut(s) 439, 882
FokI GGATG 3 cut(s) 17, 456, 895
FriOI GRGCYC 1 cut(s) 23
FspBI CTAG 2 cut(s) 144, 958
GlaI GCGC 1 cut(s) 711
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 373
HgaI GACGC 1 cut(s) 898
HhaI GCGC 1 cut(s) 712
Hin1II CATG 2 cut(s) 443, 886
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HinfI GANTC 6 cut(s) 330, 415, 595, 754, 795, 926
HphI GGTGA 1 cut(s) 871
Hpy166II GTNNAC 2 cut(s) 158, 727
Hpy188I TCNGA 6 cut(s) 226, 249, 409, 430, 691, 870
Hpy188III TCNNGA 3 cut(s) 149, 169, 790
Hpy8I GTNNAC 2 cut(s) 158, 727
HpyAV CCTTC 4 cut(s) 33, 566, 663, 696
HpyCH4III ACNGT 2 cut(s) 42, 537
HpyCH4V TGCA 7 cut(s) 434, 577, 619, 649, 670, 682, 952
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 475, 583, 700, 709
HpyF3I CTNAG 5 cut(s) 310, 492, 612, 627, 690
Hsp92II CATG 2 cut(s) 443, 886
HspAI GCGC 1 cut(s) 710
Kzo9I GATC 1 cut(s) 337
LmnI GCTCC 5 cut(s) 18, 66, 463, 661, 907
Lsp1109I GCAGC 8 cut(s) 63, 196, 303, 487, 526, 598, 601, 812
LweI GCATC 7 cut(s) 139, 243, 421, 647, 679, 697, 864
MaeI CTAG 2 cut(s) 144, 958
MaeIII GTNAC 2 cut(s) 359, 537
MalI GATC 1 cut(s) 339
MboI GATC 1 cut(s) 337
MboII GAAGA 4 cut(s) 262, 615, 829, 883
MfeI CAATTG 3 cut(s) 71, 419, 695
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 8 cut(s) 71, 174, 240, 302, 419, 487, 695, 720
MlyI GAGTC 2 cut(s) 324, 424
MmeI TCCRAC 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 241
MseI TTAA 3 cut(s) 102, 347, 746
MspR9I CCNGG 2 cut(s) 842, 944
MunI CAATTG 3 cut(s) 71, 419, 695
MvaI CCWGG 2 cut(s) 842, 944
MvnI CGCG 1 cut(s) 712
MwoI GCNNNNNNNGC 5 cut(s) 85, 475, 583, 700, 709
NcoI CCATGG 1 cut(s) 882
NdeII GATC 1 cut(s) 337
NlaIII CATG 2 cut(s) 443, 886
NlaIV GGNNCC 3 cut(s) 20, 68, 663
PaeR7I CTCGAG 1 cut(s) 788
PceI AGGCCT 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 241
PfeI GAWTC 4 cut(s) 595, 754, 795, 926
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 15
PleI GAGTC 2 cut(s) 324, 423
PpsI GAGTC 2 cut(s) 324, 423
Psp6I CCWGG 2 cut(s) 840, 942
PspGI CCWGG 2 cut(s) 840, 942
PspN4I GGNNCC 3 cut(s) 20, 68, 663
PspPI GGNCC 1 cut(s) 389
PstNI CAGNNNCTG 1 cut(s) 641
PsyI GACNNNGTC 1 cut(s) 271
RsaI GTAC 2 cut(s) 500, 527
RsaNI GTAC 2 cut(s) 499, 526
SaqAI TTAA 3 cut(s) 102, 347, 746
Sau3AI GATC 1 cut(s) 337
Sau96I GGNCC 1 cut(s) 389
ScaI AGTACT 1 cut(s) 500
SchI GAGTC 2 cut(s) 324, 424
ScrFI CCNGG 2 cut(s) 842, 944
SduI GDGCHC 1 cut(s) 23
SetI ASST 9 cut(s) 188, 296, 385, 460, 485, 655, 688, 904, 923
SfaNI GCATC 7 cut(s) 139, 243, 421, 647, 679, 697, 864
Sfr274I CTCGAG 1 cut(s) 788
SinI GGWCC 1 cut(s) 389
SlaI CTCGAG 1 cut(s) 788
SmlI CTYRAG 5 cut(s) 27, 378, 788, 809, 835
SmoI CTYRAG 5 cut(s) 27, 378, 788, 809, 835
Sse9I AATT 8 cut(s) 71, 174, 240, 302, 419, 487, 695, 720
SseBI AGGCCT 1 cut(s) 373
SsiI CCGC 3 cut(s) 88, 701, 845
SspMI CTAG 2 cut(s) 144, 958
StuI AGGCCT 1 cut(s) 373
StyD4I CCNGG 2 cut(s) 840, 942
StyI CCWWGG 1 cut(s) 882
TaaI ACNGT 2 cut(s) 42, 537
TaqI TCGA 2 cut(s) 485, 789
TasI AATT 8 cut(s) 71, 174, 240, 302, 419, 487, 695, 720
TatI WGTACW 1 cut(s) 498
TauI GCSGC 2 cut(s) 91, 703
TfiI GAWTC 4 cut(s) 595, 754, 795, 926
Tru1I TTAA 3 cut(s) 102, 347, 746
Tru9I TTAA 3 cut(s) 102, 347, 746
TseI GCWGC 8 cut(s) 76, 209, 291, 475, 514, 586, 589, 800
TspDTI ATGAA 2 cut(s) 246, 365
TspGWI ACGGA 1 cut(s) 747
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 389
XagI CCTNNNNNAGG 2 cut(s) 27, 378
XapI RAATTY 2 cut(s) 240, 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XhoI CTCGAG 1 cut(s) 788
XmnI GAANNNNTTC 1 cut(s) 241
XspI CTAG 2 cut(s) 144, 958
ZrmI AGTACT 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.