ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 2353 |
| Acc65I | GGTACC | 1 cut(s) | 1639 |
| AccB1I | GGYRCC | 1 cut(s) | 1639 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 486, 1952 |
| AccBSI | CCGCTC | 1 cut(s) | 624 |
| AccI | GTMKAC | 1 cut(s) | 1767 |
| AccII | CGCG | 1 cut(s) | 619 |
| AciI | CCGC | 8 cut(s) | 622, 1107, 1140, 1331, 1687, 1719, 1804, 2043 |
| AclI | AACGTT | 1 cut(s) | 1561 |
| AclWI | GGATC | 2 cut(s) | 60, 617 |
| AcoI | YGGCCR | 1 cut(s) | 1082 |
| AcsI | RAATTY | 3 cut(s) | 119, 1027, 1819 |
| AcuI | CTGAAG | 1 cut(s) | 320 |
| AfaI | GTAC | 9 cut(s) | 675, 703, 799, 1203, 1220, 1552, 1641, 1661, 1757 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 166, 453, 486, 1585, 1625, 1649, 1952 |
| AflIII | ACRYGT | 1 cut(s) | 1827 |
| AgsI | TTSAA | 9 cut(s) | 126, 155, 511, 778, 1045, 1574, 2014, 2182, 2353 |
| AjnI | CCWGG | 3 cut(s) | 1642, 1923, 2142 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 847, 879 |
| AleI | CACNNNNGTG | 1 cut(s) | 2228 |
| AluBI | AGCT | 8 cut(s) | 86, 94, 145, 257, 812, 959, 1286, 1669 |
| AluI | AGCT | 8 cut(s) | 86, 94, 145, 257, 812, 959, 1286, 1669 |
| Alw21I | GWGCWC | 2 cut(s) | 1470, 2211 |
| Alw26I | GTCTC | 4 cut(s) | 416, 1594, 2162, 2351 |
| Alw44I | GTGCAC | 1 cut(s) | 2207 |
| AlwI | GGATC | 2 cut(s) | 60, 617 |
| AoxI | GGCC | 3 cut(s) | 1082, 1651, 1810 |
| ApaLI | GTGCAC | 1 cut(s) | 2207 |
| ApeKI | GCWGC | 7 cut(s) | 142, 470, 1010, 1109, 1514, 1836, 2139 |
| ApoI | RAATTY | 3 cut(s) | 119, 1027, 1819 |
| AseI | ATTAAT | 1 cut(s) | 131 |
| Asp718I | GGTACC | 1 cut(s) | 1639 |
| AspLEI | GCGC | 4 cut(s) | 619, 621, 1168, 1395 |
| AspS9I | GGNCC | 2 cut(s) | 1616, 1811 |
| AsuC2I | CCSGG | 1 cut(s) | 1324 |
| AsuHPI | GGTGA | 5 cut(s) | 188, 565, 810, 1217, 1699 |
| AsuNHI | GCTAGC | 1 cut(s) | 1669 |
| AvaII | GGWCC | 1 cut(s) | 1616 |
| BaeGI | GKGCMC | 2 cut(s) | 1451, 2211 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 665, 698 |
| BalI | TGGCCA | 1 cut(s) | 1084 |
| BanI | GGYRCC | 1 cut(s) | 1639 |
| BbsI | GAAGAC | 1 cut(s) | 2160 |
| Bbv12I | GWGCWC | 2 cut(s) | 1470, 2211 |
| BbvI | GCAGC | 7 cut(s) | 154, 457, 997, 1096, 1526, 1823, 2151 |
| BccI | CCATC | 4 cut(s) | 480, 557, 1588, 1954 |
| BceAI | ACGGC | 1 cut(s) | 1344 |
| BciT130I | CCWGG | 3 cut(s) | 1644, 1925, 2144 |
| BclI | TGATCA | 1 cut(s) | 64 |
| BcnI | CCSGG | 1 cut(s) | 1324 |
| BcoDI | GTCTC | 4 cut(s) | 416, 1594, 2162, 2351 |
| BfaI | CTAG | 5 cut(s) | 536, 875, 1670, 1968, 1989 |
| BfmI | CTRYAG | 3 cut(s) | 319, 2188, 2226 |
| BfoI | RGCGCY | 2 cut(s) | 1169, 1396 |
| BfuAI | ACCTGC | 1 cut(s) | 2353 |
| BisI | GCNGC | 9 cut(s) | 143, 471, 622, 1011, 1107, 1110, 1515, 1837, 2140 |
| BlsI | GCNGC | 9 cut(s) | 144, 472, 623, 1012, 1108, 1111, 1516, 1838, 2141 |
| Bme1390I | CCNGG | 4 cut(s) | 1324, 1644, 1925, 2144 |
| Bme18I | GGWCC | 1 cut(s) | 1616 |
| BmgT120I | GGNCC | 2 cut(s) | 1616, 1811 |
| BmiI | GGNNCC | 5 cut(s) | 544, 1497, 1618, 1641, 1793 |
| BmrFI | CCNGG | 4 cut(s) | 1324, 1644, 1925, 2144 |
| BmrI | ACTGGG | 2 cut(s) | 932, 1614 |
| BmsI | GCATC | 1 cut(s) | 1698 |
| BmtI | GCTAGC | 1 cut(s) | 1673 |
| BmuI | ACTGGG | 2 cut(s) | 932, 1614 |
| BoxI | GACNNNNGTC | 1 cut(s) | 336 |
| BpiI | GAAGAC | 1 cut(s) | 2160 |
| BpmI | CTGGAG | 1 cut(s) | 561 |
| BpuEI | CTTGAG | 1 cut(s) | 1457 |
| BpuMI | CCSGG | 1 cut(s) | 1324 |
| Bsa29I | ATCGAT | 1 cut(s) | 835 |
| BsaI | GGTCTC | 1 cut(s) | 1594 |
| BsaJI | CCNNGG | 4 cut(s) | 72, 160, 697, 1187 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 166, 453, 486, 1585, 1625, 1649, 1952 |
| Bse1I | ACTGG | 6 cut(s) | 938, 1085, 1429, 1620, 1635, 1905 |
| Bse3DI | GCAATG | 1 cut(s) | 1358 |
| BseBI | CCWGG | 3 cut(s) | 1644, 1925, 2144 |
| BseCI | ATCGAT | 1 cut(s) | 835 |
| BseDI | CCNNGG | 4 cut(s) | 72, 160, 697, 1187 |
| BseGI | GGATG | 4 cut(s) | 82, 568, 1863, 2117 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 166, 453, 486, 1585, 1625, 1649, 1952 |
| BseMI | GCAATG | 1 cut(s) | 1358 |
| BseMII | CTCAG | 4 cut(s) | 618, 1524, 1656, 2163 |
| BseNI | ACTGG | 6 cut(s) | 938, 1085, 1429, 1620, 1635, 1905 |
| BsePI | GCGCGC | 1 cut(s) | 617 |
| BseRI | GAGGAG | 3 cut(s) | 183, 1406, 1460 |
| BseSI | GKGCMC | 2 cut(s) | 1451, 2211 |
| BseXI | GCAGC | 7 cut(s) | 154, 457, 997, 1096, 1526, 1823, 2151 |
| BseYI | CCCAGC | 1 cut(s) | 1491 |
| BsgI | GTGCAG | 1 cut(s) | 456 |
| Bsh1236I | CGCG | 1 cut(s) | 619 |
| BshFI | GGCC | 3 cut(s) | 1084, 1653, 1812 |
| BshNI | GGYRCC | 1 cut(s) | 1639 |
| BshVI | ATCGAT | 1 cut(s) | 835 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1470, 2211 |
| BsiSI | CCGG | 1 cut(s) | 1323 |
| BslFI | GGGAC | 4 cut(s) | 510, 913, 1339, 1602 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 166, 453, 486, 1585, 1625, 1649, 1952 |
| BsmAI | GTCTC | 4 cut(s) | 416, 1594, 2162, 2351 |
| BsmFI | GGGAC | 4 cut(s) | 510, 913, 1339, 1602 |
| BsnI | GGCC | 3 cut(s) | 1084, 1653, 1812 |
| Bso31I | GGTCTC | 1 cut(s) | 1594 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1451, 1470, 2211 |
| Bsp1407I | TGTACA | 2 cut(s) | 1201, 1659 |
| Bsp143I | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| Bsp19I | CCATGG | 2 cut(s) | 72, 697 |
| BspACI | CCGC | 8 cut(s) | 622, 1107, 1140, 1331, 1687, 1719, 1804, 2043 |
| BspANI | GGCC | 3 cut(s) | 1084, 1653, 1812 |
| BspCNI | CTCAG | 4 cut(s) | 619, 1523, 1657, 2164 |
| BspDI | ATCGAT | 1 cut(s) | 835 |
| BspFNI | CGCG | 1 cut(s) | 619 |
| BspHI | TCATGA | 4 cut(s) | 1020, 1377, 2098, 2338 |
| BspLI | GGNNCC | 5 cut(s) | 544, 1497, 1618, 1641, 1793 |
| BspMI | ACCTGC | 1 cut(s) | 2353 |
| BspOI | GCTAGC | 1 cut(s) | 1673 |
| BspPI | GGATC | 2 cut(s) | 60, 617 |
| BspT107I | GGYRCC | 1 cut(s) | 1639 |
| BspTNI | GGTCTC | 1 cut(s) | 1594 |
| BsrBI | CCGCTC | 1 cut(s) | 624 |
| BsrDI | GCAATG | 1 cut(s) | 1358 |
| BsrGI | TGTACA | 2 cut(s) | 1201, 1659 |
| BsrI | ACTGG | 6 cut(s) | 938, 1085, 1429, 1620, 1635, 1905 |
| BssECI | CCNNGG | 4 cut(s) | 72, 160, 697, 1187 |
| BssHII | GCGCGC | 1 cut(s) | 617 |
| BssMI | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| BssT1I | CCWWGG | 3 cut(s) | 72, 697, 1187 |
| Bst2UI | CCWGG | 3 cut(s) | 1644, 1925, 2144 |
| Bst6I | CTCTTC | 4 cut(s) | 108, 525, 1424, 2255 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 917 |
| BstAUI | TGTACA | 2 cut(s) | 1201, 1659 |
| BstC8I | GCNNGC | 7 cut(s) | 147, 619, 1164, 1170, 1288, 1671, 2239 |
| BstDEI | CTNAG | 7 cut(s) | 87, 95, 627, 1510, 1665, 2172, 2294 |
| BstDSI | CCRYGG | 2 cut(s) | 72, 697 |
| BstEII | GGTNACC | 1 cut(s) | 1351 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1647 |
| BstF5I | GGATG | 4 cut(s) | 82, 568, 1863, 2117 |
| BstFNI | CGCG | 1 cut(s) | 619 |
| BstH2I | RGCGCY | 2 cut(s) | 1169, 1396 |
| BstHHI | GCGC | 4 cut(s) | 619, 621, 1168, 1395 |
| BstKTI | GATC | 5 cut(s) | 55, 67, 612, 633, 1509 |
| BstMAI | GTCTC | 4 cut(s) | 416, 1594, 2162, 2351 |
| BstMBI | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 917, 1106, 1118, 1990 |
| BstNI | CCWGG | 3 cut(s) | 1644, 1925, 2144 |
| BstNSI | RCATGY | 1 cut(s) | 1831 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 336 |
| BstPI | GGTNACC | 1 cut(s) | 1351 |
| BstSCI | CCNGG | 4 cut(s) | 1322, 1642, 1923, 2142 |
| BstSFI | CTRYAG | 3 cut(s) | 319, 2188, 2226 |
| BstSLI | GKGCMC | 2 cut(s) | 1451, 2211 |
| BstUI | CGCG | 1 cut(s) | 619 |
| BstV1I | GCAGC | 7 cut(s) | 154, 457, 997, 1096, 1526, 1823, 2151 |
| BstV2I | GAAGAC | 1 cut(s) | 2160 |
| Bsu15I | ATCGAT | 1 cut(s) | 835 |
| BsuRI | GGCC | 3 cut(s) | 1084, 1653, 1812 |
| BsuTUI | ATCGAT | 1 cut(s) | 835 |
| BtgI | CCRYGG | 2 cut(s) | 72, 697 |
| BtsCI | GGATG | 4 cut(s) | 82, 568, 1863, 2117 |
| BtsIMutI | CAGTG | 7 cut(s) | 18, 693, 1092, 1422, 1662, 2223, 2323 |
| BveI | ACCTGC | 1 cut(s) | 2353 |
| Cac8I | GCNNGC | 7 cut(s) | 147, 619, 1164, 1170, 1288, 1671, 2239 |
| CciI | TCATGA | 4 cut(s) | 1020, 1377, 2098, 2338 |
| CfoI | GCGC | 4 cut(s) | 619, 621, 1168, 1395 |
| Cfr13I | GGNCC | 2 cut(s) | 1616, 1811 |
| ClaI | ATCGAT | 1 cut(s) | 835 |
| CsiI | ACCWGGT | 1 cut(s) | 1642 |
| Csp6I | GTAC | 9 cut(s) | 674, 702, 798, 1202, 1219, 1551, 1640, 1660, 1756 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1468, 1503 |
| CviQI | GTAC | 9 cut(s) | 674, 702, 798, 1202, 1219, 1551, 1640, 1660, 1756 |
| DdeI | CTNAG | 7 cut(s) | 87, 95, 627, 1510, 1665, 2172, 2294 |
| DpnI | GATC | 5 cut(s) | 54, 66, 611, 632, 1508 |
| DpnII | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| DraI | TTTAAA | 1 cut(s) | 1294 |
| EaeI | YGGCCR | 1 cut(s) | 1082 |
| Eam1104I | CTCTTC | 4 cut(s) | 108, 525, 1424, 2255 |
| EarI | CTCTTC | 4 cut(s) | 108, 525, 1424, 2255 |
| Eco130I | CCWWGG | 3 cut(s) | 72, 697, 1187 |
| Eco147I | AGGCCT | 1 cut(s) | 1653 |
| Eco31I | GGTCTC | 1 cut(s) | 1594 |
| Eco47I | GGWCC | 1 cut(s) | 1616 |
| Eco57I | CTGAAG | 1 cut(s) | 320 |
| Eco91I | GGTNACC | 1 cut(s) | 1351 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1647 |
| EcoO65I | GGTNACC | 1 cut(s) | 1351 |
| EcoRI | GAATTC | 1 cut(s) | 1027 |
| EcoRII | CCWGG | 3 cut(s) | 1642, 1923, 2142 |
| EcoT14I | CCWWGG | 3 cut(s) | 72, 697, 1187 |
| EcoT22I | ATGCAT | 1 cut(s) | 2290 |
| ErhI | CCWWGG | 3 cut(s) | 72, 697, 1187 |
| FaqI | GGGAC | 4 cut(s) | 510, 913, 1339, 1602 |
| FauI | CCCGC | 2 cut(s) | 1694, 1726 |
| FbaI | TGATCA | 1 cut(s) | 64 |
| FblI | GTMKAC | 1 cut(s) | 1767 |
| Fnu4HI | GCNGC | 9 cut(s) | 143, 471, 622, 1011, 1107, 1110, 1515, 1837, 2140 |
| FokI | GGATG | 4 cut(s) | 89, 575, 1850, 2104 |
| Fsp4HI | GCNGC | 9 cut(s) | 143, 471, 622, 1011, 1107, 1110, 1515, 1837, 2140 |
| FspBI | CTAG | 5 cut(s) | 536, 875, 1670, 1968, 1989 |
| GlaI | GCGC | 4 cut(s) | 618, 620, 1167, 1394 |
| GluI | GCNGC | 9 cut(s) | 143, 471, 622, 1011, 1107, 1110, 1515, 1837, 2140 |
| GsaI | CCCAGC | 1 cut(s) | 1495 |
| GsuI | CTGGAG | 1 cut(s) | 561 |
| HaeII | RGCGCY | 2 cut(s) | 1169, 1396 |
| HaeIII | GGCC | 3 cut(s) | 1084, 1653, 1812 |
| HapII | CCGG | 1 cut(s) | 1323 |
| HhaI | GCGC | 4 cut(s) | 619, 621, 1168, 1395 |
| Hin6I | GCGC | 4 cut(s) | 617, 619, 1166, 1393 |
| HinP1I | GCGC | 4 cut(s) | 617, 619, 1166, 1393 |
| HindIII | AAGCTT | 2 cut(s) | 92, 810 |
| HinfI | GANTC | 8 cut(s) | 568, 574, 1264, 1405, 1603, 1931, 2069, 2344 |
| HpaII | CCGG | 1 cut(s) | 1323 |
| HphI | GGTGA | 5 cut(s) | 188, 565, 810, 1217, 1699 |
| Hpy166II | GTNNAC | 7 cut(s) | 684, 1131, 1579, 1662, 1768, 1973, 2209 |
| Hpy188I | TCNGA | 6 cut(s) | 573, 628, 1033, 1610, 1960, 2125 |
| Hpy8I | GTNNAC | 7 cut(s) | 684, 1131, 1579, 1662, 1768, 1973, 2209 |
| HpyAV | CCTTC | 6 cut(s) | 8, 30, 995, 1528, 1664, 1769 |
| HpyCH4IV | ACGT | 2 cut(s) | 1561, 2009 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 917, 1106, 1118, 1990 |
| HpyF3I | CTNAG | 7 cut(s) | 87, 95, 627, 1510, 1665, 2172, 2294 |
| HpySE526I | ACGT | 2 cut(s) | 1561, 2009 |
| HspAI | GCGC | 4 cut(s) | 617, 619, 1166, 1393 |
| KpnI | GGTACC | 1 cut(s) | 1643 |
| Ksp22I | TGATCA | 1 cut(s) | 64 |
| Kzo9I | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| LmnI | GCTCC | 3 cut(s) | 254, 457, 1473 |
| Lsp1109I | GCAGC | 7 cut(s) | 154, 457, 997, 1096, 1526, 1823, 2151 |
| LweI | GCATC | 1 cut(s) | 1698 |
| MabI | ACCWGGT | 1 cut(s) | 1642 |
| MaeI | CTAG | 5 cut(s) | 536, 875, 1670, 1968, 1989 |
| MaeII | ACGT | 2 cut(s) | 1561, 2009 |
| MaeIII | GTNAC | 6 cut(s) | 233, 427, 1223, 1279, 1351, 1562 |
| MalI | GATC | 5 cut(s) | 54, 66, 611, 632, 1508 |
| MbiI | CCGCTC | 1 cut(s) | 624 |
| MboI | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| MhlI | GDGCHC | 3 cut(s) | 1451, 1470, 2211 |
| MlsI | TGGCCA | 1 cut(s) | 1084 |
| MluNI | TGGCCA | 1 cut(s) | 1084 |
| MlyI | GAGTC | 4 cut(s) | 568, 1940, 2063, 2353 |
| MmeI | TCCRAC | 3 cut(s) | 232, 471, 845 |
| Mox20I | TGGCCA | 1 cut(s) | 1084 |
| Mph1103I | ATGCAT | 1 cut(s) | 2290 |
| MscI | TGGCCA | 1 cut(s) | 1084 |
| MseI | TTAA | 6 cut(s) | 131, 755, 843, 1248, 1293, 2328 |
| MslI | CAYNNNNRTG | 2 cut(s) | 417, 2228 |
| Msp20I | TGGCCA | 1 cut(s) | 1084 |
| MspA1I | CMGCKG | 2 cut(s) | 1109, 1286 |
| MspI | CCGG | 1 cut(s) | 1323 |
| MspR9I | CCNGG | 4 cut(s) | 1324, 1644, 1925, 2144 |
| MvaI | CCWGG | 3 cut(s) | 1644, 1925, 2144 |
| MvnI | CGCG | 1 cut(s) | 619 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 917, 1106, 1118, 1990 |
| NciI | CCSGG | 1 cut(s) | 1324 |
| NcoI | CCATGG | 2 cut(s) | 72, 697 |
| NdeII | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| NheI | GCTAGC | 1 cut(s) | 1669 |
| NlaIV | GGNNCC | 5 cut(s) | 544, 1497, 1618, 1641, 1793 |
| NmuCI | GTSAC | 2 cut(s) | 233, 1223 |
| NsiI | ATGCAT | 1 cut(s) | 2290 |
| NspI | RCATGY | 1 cut(s) | 1831 |
| OliI | CACNNNNGTG | 1 cut(s) | 2228 |
| PagI | TCATGA | 4 cut(s) | 1020, 1377, 2098, 2338 |
| PauI | GCGCGC | 1 cut(s) | 617 |
| PceI | AGGCCT | 1 cut(s) | 1653 |
| PciI | ACATGT | 1 cut(s) | 1827 |
| PfeI | GAWTC | 4 cut(s) | 568, 1264, 1405, 1603 |
| PflFI | GACNNNGTC | 1 cut(s) | 1614 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 486, 1952 |
| PfoI | TCCNGGA | 1 cut(s) | 1322 |
| PkrI | GCNGC | 9 cut(s) | 144, 472, 623, 1012, 1108, 1111, 1516, 1838, 2141 |
| PleI | GAGTC | 4 cut(s) | 568, 1939, 2063, 2352 |
| PpsI | GAGTC | 4 cut(s) | 568, 1939, 2063, 2352 |
| PscI | ACATGT | 1 cut(s) | 1827 |
| PshAI | GACNNNNGTC | 1 cut(s) | 336 |
| PshBI | ATTAAT | 1 cut(s) | 131 |
| Psp1406I | AACGTT | 1 cut(s) | 1561 |
| Psp6I | CCWGG | 3 cut(s) | 1642, 1923, 2142 |
| PspEI | GGTNACC | 1 cut(s) | 1351 |
| PspFI | CCCAGC | 1 cut(s) | 1491 |
| PspGI | CCWGG | 3 cut(s) | 1642, 1923, 2142 |
| PspN4I | GGNNCC | 5 cut(s) | 544, 1497, 1618, 1641, 1793 |
| PspPI | GGNCC | 2 cut(s) | 1616, 1811 |
| PsyI | GACNNNGTC | 1 cut(s) | 1614 |
| PteI | GCGCGC | 1 cut(s) | 617 |
| PvuII | CAGCTG | 1 cut(s) | 1286 |
| RsaI | GTAC | 9 cut(s) | 675, 703, 799, 1203, 1220, 1552, 1641, 1661, 1757 |
| RsaNI | GTAC | 9 cut(s) | 674, 702, 798, 1202, 1219, 1551, 1640, 1660, 1756 |
| RseI | CAYNNNNRTG | 2 cut(s) | 417, 2228 |
| SaqAI | TTAA | 6 cut(s) | 131, 755, 843, 1248, 1293, 2328 |
| SatI | GCNGC | 9 cut(s) | 143, 471, 622, 1011, 1107, 1110, 1515, 1837, 2140 |
| Sau3AI | GATC | 5 cut(s) | 52, 64, 609, 630, 1506 |
| Sau96I | GGNCC | 2 cut(s) | 1616, 1811 |
| SchI | GAGTC | 4 cut(s) | 568, 1940, 2063, 2353 |
| ScrFI | CCNGG | 4 cut(s) | 1324, 1644, 1925, 2144 |
| SduI | GDGCHC | 3 cut(s) | 1451, 1470, 2211 |
| SexAI | ACCWGGT | 1 cut(s) | 1642 |
| SfaNI | GCATC | 1 cut(s) | 1698 |
| SfcI | CTRYAG | 3 cut(s) | 319, 2188, 2226 |
| SinI | GGWCC | 1 cut(s) | 1616 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 417, 2228 |
| SmlI | CTYRAG | 1 cut(s) | 1472 |
| SmoI | CTYRAG | 1 cut(s) | 1472 |
| SseBI | AGGCCT | 1 cut(s) | 1653 |
| SsiI | CCGC | 8 cut(s) | 622, 1107, 1140, 1331, 1687, 1719, 1804, 2043 |
| SspI | AATATT | 1 cut(s) | 507 |
| SspMI | CTAG | 5 cut(s) | 536, 875, 1670, 1968, 1989 |
| StuI | AGGCCT | 1 cut(s) | 1653 |
| StyD4I | CCNGG | 4 cut(s) | 1322, 1642, 1923, 2142 |
| StyI | CCWWGG | 3 cut(s) | 72, 697, 1187 |
| TaiI | ACGT | 2 cut(s) | 1564, 2012 |
| TaqI | TCGA | 5 cut(s) | 376, 835, 1592, 2063, 2151 |
| TatI | WGTACW | 2 cut(s) | 1201, 1659 |
| TauI | GCSGC | 2 cut(s) | 624, 1109 |
| TfiI | GAWTC | 4 cut(s) | 568, 1264, 1405, 1603 |
| Tru1I | TTAA | 6 cut(s) | 131, 755, 843, 1248, 1293, 2328 |
| Tru9I | TTAA | 6 cut(s) | 131, 755, 843, 1248, 1293, 2328 |
| TscAI | CASTG | 7 cut(s) | 25, 693, 1092, 1429, 1669, 2230, 2323 |
| TseFI | GTSAC | 2 cut(s) | 233, 1223 |
| TseI | GCWGC | 7 cut(s) | 142, 470, 1010, 1109, 1514, 1836, 2139 |
| Tsp45I | GTSAC | 2 cut(s) | 233, 1223 |
| TspDTI | ATGAA | 8 cut(s) | 27, 93, 126, 354, 1037, 1715, 2087, 2115 |
| TspRI | CASTG | 7 cut(s) | 25, 693, 1092, 1429, 1669, 2230, 2323 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1614 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 486, 1952 |
| VneI | GTGCAC | 1 cut(s) | 2207 |
| VpaK11BI | GGWCC | 1 cut(s) | 1616 |
| VspI | ATTAAT | 1 cut(s) | 131 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1647 |
| XapI | RAATTY | 3 cut(s) | 119, 1027, 1819 |
| XceI | RCATGY | 1 cut(s) | 1831 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 487, 1248, 1627 |
| XmiI | GTMKAC | 1 cut(s) | 1767 |
| XspI | CTAG | 5 cut(s) | 536, 875, 1670, 1968, 1989 |
| Zsp2I | ATGCAT | 1 cut(s) | 2290 |