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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 129 |
| Acc36I | ACCTGC | 1 cut(s) | 129 |
| AccB1I | GGYRCC | 2 cut(s) | 4, 496 |
| AccI | GTMKAC | 1 cut(s) | 130 |
| AccII | CGCG | 1 cut(s) | 474 |
| AciI | CCGC | 1 cut(s) | 145 |
| AclWI | GGATC | 1 cut(s) | 31 |
| AcoI | YGGCCR | 2 cut(s) | 213, 397 |
| AcsI | RAATTY | 1 cut(s) | 380 |
| AcyI | GRCGYC | 1 cut(s) | 224 |
| AfaI | GTAC | 1 cut(s) | 111 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 259, 384 |
| AgsI | TTSAA | 5 cut(s) | 93, 201, 278, 286, 424 |
| AjnI | CCWGG | 2 cut(s) | 321, 394 |
| AluBI | AGCT | 1 cut(s) | 442 |
| AluI | AGCT | 1 cut(s) | 442 |
| Alw21I | GWGCWC | 1 cut(s) | 272 |
| AlwI | GGATC | 1 cut(s) | 31 |
| AoxI | GGCC | 3 cut(s) | 43, 213, 397 |
| ApeKI | GCWGC | 2 cut(s) | 205, 290 |
| ApoI | RAATTY | 1 cut(s) | 380 |
| AspLEI | GCGC | 3 cut(s) | 138, 474, 476 |
| BaeGI | GKGCMC | 1 cut(s) | 321 |
| BalI | TGGCCA | 1 cut(s) | 215 |
| BanI | GGYRCC | 2 cut(s) | 4, 496 |
| Bbv12I | GWGCWC | 1 cut(s) | 272 |
| BbvI | GCAGC | 2 cut(s) | 192, 277 |
| BccI | CCATC | 1 cut(s) | 193 |
| BceAI | ACGGC | 1 cut(s) | 149 |
| BciT130I | CCWGG | 2 cut(s) | 323, 396 |
| BfaI | CTAG | 3 cut(s) | 237, 249, 356 |
| BfmI | CTRYAG | 1 cut(s) | 189 |
| BfuAI | ACCTGC | 1 cut(s) | 129 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 221 |
| BisI | GCNGC | 2 cut(s) | 206, 291 |
| BlsI | GCNGC | 2 cut(s) | 207, 292 |
| Bme1390I | CCNGG | 2 cut(s) | 323, 396 |
| BmiI | GGNNCC | 3 cut(s) | 6, 327, 498 |
| BmrFI | CCNGG | 2 cut(s) | 323, 396 |
| BmsI | GCATC | 1 cut(s) | 317 |
| BpmI | CTGGAG | 2 cut(s) | 297, 486 |
| BpuEI | CTTGAG | 2 cut(s) | 422, 499 |
| BsaHI | GRCGYC | 1 cut(s) | 224 |
| BsaJI | CCNNGG | 2 cut(s) | 40, 321 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 259, 384 |
| Bse1I | ACTGG | 3 cut(s) | 24, 209, 314 |
| BseBI | CCWGG | 2 cut(s) | 323, 396 |
| BseDI | CCNNGG | 2 cut(s) | 40, 321 |
| BseGI | GGATG | 2 cut(s) | 177, 529 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 259, 384 |
| BseNI | ACTGG | 3 cut(s) | 24, 209, 314 |
| BsePI | GCGCGC | 1 cut(s) | 472 |
| BseSI | GKGCMC | 1 cut(s) | 321 |
| BseXI | GCAGC | 2 cut(s) | 192, 277 |
| Bsh1236I | CGCG | 1 cut(s) | 474 |
| BshFI | GGCC | 3 cut(s) | 45, 215, 399 |
| BshNI | GGYRCC | 2 cut(s) | 4, 496 |
| BsiHKAI | GWGCWC | 1 cut(s) | 272 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 259, 384 |
| BsmI | GAATGC | 1 cut(s) | 388 |
| BsnI | GGCC | 3 cut(s) | 45, 215, 399 |
| Bsp1286I | GDGCHC | 2 cut(s) | 272, 321 |
| Bsp143I | GATC | 1 cut(s) | 36 |
| BspACI | CCGC | 1 cut(s) | 145 |
| BspANI | GGCC | 3 cut(s) | 45, 215, 399 |
| BspFNI | CGCG | 1 cut(s) | 474 |
| BspLI | GGNNCC | 3 cut(s) | 6, 327, 498 |
| BspMAI | CTGCAG | 1 cut(s) | 193 |
| BspMI | ACCTGC | 1 cut(s) | 129 |
| BspPI | GGATC | 1 cut(s) | 31 |
| BspT107I | GGYRCC | 2 cut(s) | 4, 496 |
| BsrI | ACTGG | 3 cut(s) | 24, 209, 314 |
| BssECI | CCNNGG | 2 cut(s) | 40, 321 |
| BssHII | GCGCGC | 1 cut(s) | 472 |
| BssMI | GATC | 1 cut(s) | 36 |
| BssNI | GRCGYC | 1 cut(s) | 224 |
| BssT1I | CCWWGG | 1 cut(s) | 40 |
| Bst2UI | CCWGG | 2 cut(s) | 323, 396 |
| Bst4CI | ACNGT | 3 cut(s) | 197, 274, 557 |
| BstACI | GRCGYC | 1 cut(s) | 224 |
| BstC8I | GCNNGC | 3 cut(s) | 54, 474, 486 |
| BstDEI | CTNAG | 1 cut(s) | 429 |
| BstF5I | GGATG | 2 cut(s) | 177, 529 |
| BstFNI | CGCG | 1 cut(s) | 474 |
| BstHHI | GCGC | 3 cut(s) | 138, 474, 476 |
| BstKTI | GATC | 1 cut(s) | 39 |
| BstMBI | GATC | 1 cut(s) | 36 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 142, 221 |
| BstNI | CCWGG | 2 cut(s) | 323, 396 |
| BstSCI | CCNGG | 2 cut(s) | 321, 394 |
| BstSFI | CTRYAG | 1 cut(s) | 189 |
| BstSLI | GKGCMC | 1 cut(s) | 321 |
| BstUI | CGCG | 1 cut(s) | 474 |
| BstV1I | GCAGC | 2 cut(s) | 192, 277 |
| BstX2I | RGATCY | 1 cut(s) | 36 |
| BstYI | RGATCY | 1 cut(s) | 36 |
| BsuRI | GGCC | 3 cut(s) | 45, 215, 399 |
| BtsCI | GGATG | 2 cut(s) | 177, 529 |
| BtsIMutI | CAGTG | 5 cut(s) | 111, 216, 227, 321, 562 |
| BveI | ACCTGC | 1 cut(s) | 129 |
| Cac8I | GCNNGC | 3 cut(s) | 54, 474, 486 |
| CfoI | GCGC | 3 cut(s) | 138, 474, 476 |
| CseI | GACGC | 1 cut(s) | 213 |
| Csp6I | GTAC | 1 cut(s) | 110 |
| CviAII | CATG | 2 cut(s) | 116, 520 |
| CviQI | GTAC | 1 cut(s) | 110 |
| DdeI | CTNAG | 1 cut(s) | 429 |
| DpnI | GATC | 1 cut(s) | 38 |
| DpnII | GATC | 1 cut(s) | 36 |
| EaeI | YGGCCR | 2 cut(s) | 213, 397 |
| Eco130I | CCWWGG | 1 cut(s) | 40 |
| EcoRII | CCWGG | 2 cut(s) | 321, 394 |
| EcoT14I | CCWWGG | 1 cut(s) | 40 |
| ErhI | CCWWGG | 1 cut(s) | 40 |
| FaeI | CATG | 2 cut(s) | 119, 523 |
| FaiI | YATR | 3 cut(s) | 117, 512, 521 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 461, 493 |
| FatI | CATG | 2 cut(s) | 115, 519 |
| FblI | GTMKAC | 1 cut(s) | 130 |
| Fnu4HI | GCNGC | 2 cut(s) | 206, 291 |
| FokI | GGATG | 2 cut(s) | 184, 536 |
| Fsp4HI | GCNGC | 2 cut(s) | 206, 291 |
| FspBI | CTAG | 3 cut(s) | 237, 249, 356 |
| GlaI | GCGC | 3 cut(s) | 137, 473, 475 |
| GluI | GCNGC | 2 cut(s) | 206, 291 |
| GsuI | CTGGAG | 2 cut(s) | 297, 486 |
| HaeIII | GGCC | 3 cut(s) | 45, 215, 399 |
| HgaI | GACGC | 1 cut(s) | 213 |
| HhaI | GCGC | 3 cut(s) | 138, 474, 476 |
| Hin1I | GRCGYC | 1 cut(s) | 224 |
| Hin1II | CATG | 2 cut(s) | 119, 523 |
| Hin6I | GCGC | 3 cut(s) | 136, 472, 474 |
| HinP1I | GCGC | 3 cut(s) | 136, 472, 474 |
| HincII | GTYRAC | 1 cut(s) | 553 |
| HindII | GTYRAC | 1 cut(s) | 553 |
| Hpy166II | GTNNAC | 2 cut(s) | 131, 553 |
| Hpy188III | TCNNGA | 1 cut(s) | 503 |
| Hpy8I | GTNNAC | 2 cut(s) | 131, 553 |
| HpyAV | CCTTC | 1 cut(s) | 280 |
| HpyCH4III | ACNGT | 3 cut(s) | 197, 274, 557 |
| HpyCH4V | TGCA | 3 cut(s) | 159, 191, 368 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 142, 221 |
| HpyF3I | CTNAG | 1 cut(s) | 429 |
| Hsp92I | GRCGYC | 1 cut(s) | 224 |
| Hsp92II | CATG | 2 cut(s) | 119, 523 |
| HspAI | GCGC | 3 cut(s) | 136, 472, 474 |
| Kzo9I | GATC | 1 cut(s) | 36 |
| LmnI | GCTCC | 2 cut(s) | 298, 331 |
| Lsp1109I | GCAGC | 2 cut(s) | 192, 277 |
| LweI | GCATC | 1 cut(s) | 317 |
| MaeI | CTAG | 3 cut(s) | 237, 249, 356 |
| MaeIII | GTNAC | 1 cut(s) | 225 |
| MalI | GATC | 1 cut(s) | 38 |
| MboI | GATC | 1 cut(s) | 36 |
| MboII | GAAGA | 2 cut(s) | 414, 478 |
| MflI | RGATCY | 1 cut(s) | 36 |
| MhlI | GDGCHC | 2 cut(s) | 272, 321 |
| MlsI | TGGCCA | 1 cut(s) | 215 |
| MluCI | AATT | 2 cut(s) | 380, 458 |
| MluNI | TGGCCA | 1 cut(s) | 215 |
| MmeI | TCCRAC | 1 cut(s) | 156 |
| MnlI | CCTC | 7 cut(s) | 56, 61, 456, 474, 487, 508, 510 |
| Mox20I | TGGCCA | 1 cut(s) | 215 |
| MscI | TGGCCA | 1 cut(s) | 215 |
| MslI | CAYNNNNRTG | 1 cut(s) | 524 |
| Msp20I | TGGCCA | 1 cut(s) | 215 |
| MspR9I | CCNGG | 2 cut(s) | 323, 396 |
| Mva1269I | GAATGC | 1 cut(s) | 388 |
| MvaI | CCWGG | 2 cut(s) | 323, 396 |
| MvnI | CGCG | 1 cut(s) | 474 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 142, 221 |
| NdeII | GATC | 1 cut(s) | 36 |
| NlaIII | CATG | 2 cut(s) | 119, 523 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 327, 498 |
| NmuCI | GTSAC | 1 cut(s) | 225 |
| PaqCI | CACCTGC | 1 cut(s) | 129 |
| PauI | GCGCGC | 1 cut(s) | 472 |
| PctI | GAATGC | 1 cut(s) | 388 |
| PkrI | GCNGC | 2 cut(s) | 207, 292 |
| Psp6I | CCWGG | 2 cut(s) | 321, 394 |
| PspGI | CCWGG | 2 cut(s) | 321, 394 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 327, 498 |
| PstI | CTGCAG | 1 cut(s) | 193 |
| PsuI | RGATCY | 1 cut(s) | 36 |
| PteI | GCGCGC | 1 cut(s) | 472 |
| RsaI | GTAC | 1 cut(s) | 111 |
| RsaNI | GTAC | 1 cut(s) | 110 |
| RseI | CAYNNNNRTG | 1 cut(s) | 524 |
| SatI | GCNGC | 2 cut(s) | 206, 291 |
| Sau3AI | GATC | 1 cut(s) | 36 |
| ScrFI | CCNGG | 2 cut(s) | 323, 396 |
| SduI | GDGCHC | 2 cut(s) | 272, 321 |
| SetI | ASST | 4 cut(s) | 143, 420, 444, 498 |
| SfaNI | GCATC | 1 cut(s) | 317 |
| SfcI | CTRYAG | 1 cut(s) | 189 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 524 |
| SmlI | CTYRAG | 2 cut(s) | 437, 478 |
| SmoI | CTYRAG | 2 cut(s) | 437, 478 |
| Sse9I | AATT | 2 cut(s) | 380, 458 |
| SsiI | CCGC | 1 cut(s) | 145 |
| SspMI | CTAG | 3 cut(s) | 237, 249, 356 |
| StyD4I | CCNGG | 2 cut(s) | 321, 394 |
| StyI | CCWWGG | 1 cut(s) | 40 |
| TaaI | ACNGT | 3 cut(s) | 197, 274, 557 |
| TasI | AATT | 2 cut(s) | 380, 458 |
| TscAI | CASTG | 5 cut(s) | 111, 216, 234, 321, 562 |
| TseFI | GTSAC | 1 cut(s) | 225 |
| TseI | GCWGC | 2 cut(s) | 205, 290 |
| Tsp45I | GTSAC | 1 cut(s) | 225 |
| TspRI | CASTG | 5 cut(s) | 111, 216, 234, 321, 562 |
| XapI | RAATTY | 1 cut(s) | 380 |
| XmiI | GTMKAC | 1 cut(s) | 130 |
| XspI | CTAG | 3 cut(s) | 237, 249, 356 |