pycom04g21590

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001919
Physical Location & Seq
Forward (+)
22681067 .. 22681395
329 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g21590.1

Sequence Viewer

Length: 240 bp
ATGCCAAGAGAGTTGAGTCCGGGCTTGAAGATCCTCTGGGTCTGGACACTTGGCACCGCCGCCGTACTGGTCACCAGTGTGGTGAGGACGAGGATGAGAGACATGGAGAATCTTACCAACACCGAGCAGCAGCAGCCGCAGCCGCAGCTGCAGCAGCACGACACTGCCGCCGACACGGAGACACTCATGGCAGACACTTCGACTCAGTCCAATGAGCTCATTCGAGAAGATAAACCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

80

Amino Acids

8.96

Weight (kDa)

4.72

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 53
AciI CCGC 5 cut(s) 57, 60, 137, 143, 168
AclWI GGATC 1 cut(s) 25
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 77
AluBI AGCT 2 cut(s) 148, 217
AluI AGCT 2 cut(s) 148, 217
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 2 cut(s) 93, 173
AlwI GGATC 1 cut(s) 25
ApeKI GCWGC 8 cut(s) 127, 130, 133, 139, 145, 148, 151, 154
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 64, 94
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 8 cut(s) 135, 139, 142, 145, 151, 157, 163, 166
BceAI ACGGC 1 cut(s) 47
BcgI CGANNNNNNTGC 2 cut(s) 180, 214
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 2 cut(s) 93, 173
BfmI CTRYAG 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 21
Bse1I ACTGG 2 cut(s) 72, 75
BseGI GGATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 2 cut(s) 72, 75
BseXI GCAGC 8 cut(s) 135, 139, 142, 145, 151, 157, 163, 166
BshNI GGYRCC 1 cut(s) 53
BsiHKAI GWGCWC 1 cut(s) 219
BsiSI CCGG 1 cut(s) 20
BsmAI GTCTC 2 cut(s) 93, 173
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 30
BspACI CCGC 5 cut(s) 57, 60, 137, 143, 168
BspCNI CTCAG 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 153
BspPI GGATC 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 53
BsrI ACTGG 2 cut(s) 72, 75
BssMI GATC 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 204
BstEII GGTNACC 1 cut(s) 70
BstF5I GGATG 1 cut(s) 99
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 2 cut(s) 93, 173
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 8 cut(s) 133, 136, 139, 142, 145, 148, 151, 154
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 149
BstV1I GCAGC 8 cut(s) 135, 139, 142, 145, 151, 157, 163, 166
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BtsCI GGATG 1 cut(s) 99
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 82, 162
Csp6I GTAC 1 cut(s) 65
CviAII CATG 2 cut(s) 103, 187
CviJI RGCY 5 cut(s) 24, 136, 142, 148, 217
CviKI_1 RGCY 5 cut(s) 24, 136, 142, 148, 217
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 204
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 217
Eco91I GGTNACC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 217
EcoO65I GGTNACC 1 cut(s) 70
EcoT38I GRGCYC 1 cut(s) 219
FaeI CATG 2 cut(s) 106, 190
FaiI YATR 2 cut(s) 104, 188
FatI CATG 2 cut(s) 102, 186
FokI GGATG 1 cut(s) 106
FriOI GRGCYC 1 cut(s) 219
HapII CCGG 1 cut(s) 20
Hin1II CATG 2 cut(s) 106, 190
HinfI GANTC 3 cut(s) 16, 109, 202
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 64, 94
Hpy188III TCNNGA 2 cut(s) 43, 224
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 151
HpyF10VI GCNNNNNNNGC 8 cut(s) 133, 136, 139, 142, 145, 148, 151, 154
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 2 cut(s) 106, 190
Kzo9I GATC 1 cut(s) 30
LpnPI CCDG 5 cut(s) 22, 28, 33, 53, 88
Lsp1109I GCAGC 8 cut(s) 135, 139, 142, 145, 151, 157, 163, 166
MaeIII GTNAC 1 cut(s) 70
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 2 cut(s) 40, 239
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 219
MlyI GAGTC 2 cut(s) 25, 196
MnlI CCTC 3 cut(s) 44, 78, 84
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 148
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 1 cut(s) 21
MwoI GCNNNNNNNGC 8 cut(s) 133, 136, 139, 142, 145, 148, 151, 154
NciI CCSGG 1 cut(s) 21
NdeII GATC 1 cut(s) 30
NlaIII CATG 2 cut(s) 106, 190
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 70
OliI CACNNNNGTG 1 cut(s) 77
PfeI GAWTC 1 cut(s) 109
PflFI GACNNNGTC 1 cut(s) 205
PleI GAGTC 2 cut(s) 24, 196
PpsI GAGTC 2 cut(s) 24, 196
Psp124BI GAGCTC 1 cut(s) 219
PspEI GGTNACC 1 cut(s) 70
PspN4I GGNNCC 1 cut(s) 55
PstI CTGCAG 1 cut(s) 153
PsuI RGATCY 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 77
SacI GAGCTC 1 cut(s) 219
Sau3AI GATC 1 cut(s) 30
SchI GAGTC 2 cut(s) 25, 196
ScrFI CCNGG 1 cut(s) 21
SduI GDGCHC 1 cut(s) 219
SetI ASST 2 cut(s) 150, 219
SfcI CTRYAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 77
SsiI CCGC 5 cut(s) 57, 60, 137, 143, 168
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 200, 223
TauI GCSGC 4 cut(s) 62, 139, 145, 170
TfiI GAWTC 1 cut(s) 109
TscAI CASTG 2 cut(s) 82, 169
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 8 cut(s) 127, 130, 133, 139, 145, 148, 151, 154
Tsp45I GTSAC 1 cut(s) 70
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 82, 169
Tth111I GACNNNGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.