MD12G1086700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001919
Physical Location & Seq
Reverse (-)
10643749 .. 10644439
691 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1086700.v1.1.491

Sequence Viewer

Length: 219 bp
ATGCGAAGAGAGTTGAGTCCCGGCTTGAAGATCCTCTGGGTCTGGACACTTGGAACCGCCGCCGTACTTGTTACCAGTGTGGTGAGGACAAGGATGAGGGACATGGAAACTCTTACCAACACCGAGCATCAGCCGCAGCCGCAGCAACACGACACGGAGACACTGATGGCGGACAGTTCGACTCAGTCCAGTGAACTGATTCGAGAAGAGAAGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

73

Amino Acids

8.26

Weight (kDa)

5.57

Isoelectric Point (pI)

51.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 57, 60, 134, 140, 170
AclWI GGATC 1 cut(s) 25
AfaI GTAC 1 cut(s) 66
AgsI TTSAA 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 25
ApeKI GCWGC 2 cut(s) 136, 142
Asp700I GAANNNNTTC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 1 cut(s) 94
BbvI GCAGC 2 cut(s) 148, 154
BccI CCATC 1 cut(s) 160
BceAI ACGGC 2 cut(s) 47, 199
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 152
BisI GCNGC 5 cut(s) 60, 134, 137, 140, 143
BlsI GCNGC 5 cut(s) 61, 135, 138, 141, 144
Bme1390I CCNGG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 136
BpuMI CCSGG 1 cut(s) 21
Bse1I ACTGG 2 cut(s) 75, 189
BseGI GGATG 1 cut(s) 99
BseMII CTCAG 1 cut(s) 197
BseNI ACTGG 2 cut(s) 75, 189
BseXI GCAGC 2 cut(s) 148, 154
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 2 cut(s) 3, 113
BsmAI GTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 3, 113
Bsp143I GATC 1 cut(s) 30
BspACI CCGC 5 cut(s) 57, 60, 134, 140, 170
BspCNI CTCAG 1 cut(s) 196
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 25
BsrI ACTGG 2 cut(s) 75, 189
BssMI GATC 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 99
BstKTI GATC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 133, 139, 142
BstSCI CCNGG 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 148, 154
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BtsCI GGATG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 82, 161, 196
Csp6I GTAC 1 cut(s) 65
CviAII CATG 1 cut(s) 103
CviJI RGCY 4 cut(s) 24, 133, 139, 214
CviKI_1 RGCY 4 cut(s) 24, 133, 139, 214
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 1 cut(s) 183
DpnI GATC 1 cut(s) 32
DpnII GATC 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 185
FaeI CATG 1 cut(s) 106
FaiI YATR 1 cut(s) 104
FaqI GGGAC 2 cut(s) 3, 113
FatI CATG 1 cut(s) 102
Fnu4HI GCNGC 5 cut(s) 60, 134, 137, 140, 143
FokI GGATG 1 cut(s) 106
Fsp4HI GCNGC 5 cut(s) 60, 134, 137, 140, 143
GluI GCNGC 5 cut(s) 60, 134, 137, 140, 143
HapII CCGG 1 cut(s) 21
Hin1II CATG 1 cut(s) 106
HinfI GANTC 3 cut(s) 16, 181, 199
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 1 cut(s) 194
Hpy188III TCNNGA 2 cut(s) 43, 203
Hpy8I GTNNAC 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 139, 142
HpyF3I CTNAG 1 cut(s) 183
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 1 cut(s) 30
LpnPI CCDG 5 cut(s) 22, 28, 34, 88, 202
Lsp1109I GCAGC 2 cut(s) 148, 154
LweI GCATC 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 70
MalI GATC 1 cut(s) 32
MboI GATC 1 cut(s) 30
MboII GAAGA 3 cut(s) 18, 40, 218
MflI RGATCY 1 cut(s) 30
MlyI GAGTC 2 cut(s) 25, 175
MnlI CCTC 3 cut(s) 44, 78, 90
MroXI GAANNNNTTC 1 cut(s) 198
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 133, 139, 142
NciI CCSGG 1 cut(s) 21
NdeII GATC 1 cut(s) 30
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 1 cut(s) 199
PflFI GACNNNGTC 1 cut(s) 184
PkrI GCNGC 5 cut(s) 61, 135, 138, 141, 144
PleI GAGTC 2 cut(s) 24, 175
PpsI GAGTC 2 cut(s) 24, 175
PspN4I GGNNCC 1 cut(s) 55
PsuI RGATCY 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 184
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SatI GCNGC 5 cut(s) 60, 134, 137, 140, 143
Sau3AI GATC 1 cut(s) 30
SchI GAGTC 2 cut(s) 25, 175
ScrFI CCNGG 1 cut(s) 21
SfaNI GCATC 1 cut(s) 136
SsiI CCGC 5 cut(s) 57, 60, 134, 140, 170
StyD4I CCNGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 2 cut(s) 179, 202
TauI GCSGC 3 cut(s) 62, 136, 142
TfiI GAWTC 1 cut(s) 199
TscAI CASTG 3 cut(s) 82, 168, 196
TseI GCWGC 2 cut(s) 136, 142
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 82, 168, 196
Tth111I GACNNNGTC 1 cut(s) 184
XmnI GAANNNNTTC 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.