Prupe.6G361600_v2.0.a1

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011677
Physical Location & Seq
Reverse (-)
30462534 .. 30464943
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G361600.1

Sequence Viewer

Length: 501 bp
ATGGCCTTGGTAACAACTGCTGAAGTTTGCGACGCACACTCGCAGCTAATTGGAAGTGGTGATCTACGGGTGCTTGAGCCAATATTTCAGATATATGGCAGGCGACAGGTCTTCTCTGGACAAATAGTTACCTTAAAGGTATTTGAAGACAACGTTCTGGTTCGTGGGTTTCTCGAGGAGAAAGGCAATGGCAGAGTTCTTGTTGTGGATGGGGGTGGAAGCAAGCGGTGTGCAATATTGGGGGGCAACCCTGTAGTTCAAGCTCAGAATAATGGATGGGCAGGTATAGTGGTGAATGGCTGTATAAGAGATGTTGATGAGATTAATGGTTGTGACATTGGTGTGAGAGCTCTAGCGTCCCATCCCATGAAAGCCAATAAGAAAGGGATTGGAGAGAAACAGGTACCAATTACCATCGCTGGGACAAGGATCTGCGATGGGGAATGGCTTTATGCAGACACCGATGGAATTCTGATTTCTCGTACCGAGTTATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.73

Weight (kDa)

5.69

Isoelectric Point (pI)

23.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AciI CCGC 1 cut(s) 226
AclI AACGTT 1 cut(s) 153
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 405, 484
AfiI CCNNNNNNNGG 1 cut(s) 420
AgsI TTSAA 2 cut(s) 146, 260
AluBI AGCT 3 cut(s) 46, 263, 350
AluI AGCT 3 cut(s) 46, 263, 350
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 1 cut(s) 437
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 1 cut(s) 468
AseI ATTAAT 1 cut(s) 324
Asp718I GGTACC 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 71, 304
AvaI CYCGRG 1 cut(s) 173
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 2 cut(s) 103, 153
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 1 cut(s) 55
BccI CCATC 6 cut(s) 203, 270, 369, 422, 431, 458
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 252
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
BmeT110I CYCGRG 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 405
BpiI GAAGAC 2 cut(s) 103, 153
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 6
Bsc4I CCNNNNNNNGG 1 cut(s) 420
Bse3DI GCAATG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 3 cut(s) 214, 281, 361
BseLI CCNNNNNNNGG 1 cut(s) 420
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 55
BseYI CCCAGC 1 cut(s) 419
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 352
BsiHKCI CYCGRG 1 cut(s) 173
BslFI GGGAC 2 cut(s) 343, 436
BslI CCNNNNNNNGG 1 cut(s) 420
BsmFI GGGAC 2 cut(s) 343, 436
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 2 cut(s) 61, 429
BspACI CCGC 1 cut(s) 226
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 405
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 2 cut(s) 61, 429
BssT1I CCWWGG 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 101, 224
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 3 cut(s) 214, 281, 361
BstKTI GATC 2 cut(s) 64, 432
BstMBI GATC 2 cut(s) 61, 429
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 2 cut(s) 103, 153
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 2 cut(s) 400, 450
BtsCI GGATG 3 cut(s) 214, 281, 361
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 2 cut(s) 101, 224
CseI GACGC 2 cut(s) 41, 345
Csp6I GTAC 2 cut(s) 404, 483
CviAII CATG 1 cut(s) 367
CviJI RGCY 8 cut(s) 5, 46, 79, 263, 300, 350, 374, 448
CviKI_1 RGCY 8 cut(s) 5, 46, 79, 263, 300, 350, 374, 448
CviQI GTAC 2 cut(s) 404, 483
DdeI CTNAG 1 cut(s) 264
DpnI GATC 2 cut(s) 63, 431
DpnII GATC 2 cut(s) 61, 429
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 1 cut(s) 370
FaiI YATR 7 cut(s) 94, 96, 287, 305, 368, 453, 499
FaqI GGGAC 2 cut(s) 343, 436
FatI CATG 1 cut(s) 366
Fnu4HI GCNGC 1 cut(s) 44
FokI GGATG 3 cut(s) 221, 288, 348
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 1 cut(s) 44
FspBI CTAG 1 cut(s) 353
GluI GCNGC 1 cut(s) 44
GsaI CCCAGC 1 cut(s) 423
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 41, 345
Hin1II CATG 1 cut(s) 370
HphI GGTGA 2 cut(s) 71, 304
Hpy188I TCNGA 3 cut(s) 90, 267, 474
Hpy188III TCNNGA 2 cut(s) 117, 173
Hpy99I CGWCG 1 cut(s) 35
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 233, 455
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 1 cut(s) 370
KpnI GGTACC 1 cut(s) 407
Kzo9I GATC 2 cut(s) 61, 429
LpnPI CCDG 8 cut(s) 85, 92, 102, 143, 264, 267, 386, 405
Lsp1109I GCAGC 1 cut(s) 55
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 3 cut(s) 10, 127, 332
MalI GATC 2 cut(s) 63, 431
MboI GATC 2 cut(s) 61, 429
MboII GAAGA 2 cut(s) 103, 158
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 3 cut(s) 48, 408, 468
MnlI CCTC 1 cut(s) 169
MseI TTAA 2 cut(s) 134, 324
MslI CAYNNNNRTG 1 cut(s) 341
NdeII GATC 2 cut(s) 61, 429
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 173
PkrI GCNGC 1 cut(s) 45
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 352
Psp1406I AACGTT 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 419
PspN4I GGNNCC 1 cut(s) 405
PsuI RGATCY 1 cut(s) 429
RsaI GTAC 2 cut(s) 405, 484
RsaNI GTAC 2 cut(s) 404, 483
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 2 cut(s) 134, 324
SatI GCNGC 1 cut(s) 44
Sau3AI GATC 2 cut(s) 61, 429
SduI GDGCHC 1 cut(s) 352
SetI ASST 9 cut(s) 48, 111, 134, 141, 156, 265, 286, 352, 405
SfcI CTRYAG 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 74, 173
SmoI CTYRAG 2 cut(s) 74, 173
Sse9I AATT 3 cut(s) 48, 408, 468
SsiI CCGC 1 cut(s) 226
SspI AATATT 2 cut(s) 84, 237
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 6
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 48, 408, 468
Tru1I TTAA 2 cut(s) 134, 324
Tru9I TTAA 2 cut(s) 134, 324
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 383
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 1 cut(s) 468
XhoI CTCGAG 1 cut(s) 173
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.