MD04G1245000.v1.1

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011677
Physical Location & Seq
Reverse (-)
32081734 .. 32084024
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1245000.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGCCTTGGTTACAACTGCTGAAGTTTGTGACGCTCACTCGCAGTTGATTGGAAGTGGTGATCTTCGGGTGCTTGAGCCAGCATTCCAGATATATGGCCGGCGACAGGTGTTCTCTGGACAAATAGTTACCTTAAAGGTATTTGAAGACAATGTCCTTGTTCGTGGGTTTCTCGAGGAGAAGGGAAACGGCAGAGTCCTTGTTGTGGATGGGGGTGGAAGCAAGCGGTGCGCAATATTGGGAGGCAACCCTGTAGTCCAAGCTCAGAACAACGGATGGGCAGGGATAGTGGTCAATGGCTGTATAAGAGATGTCGACGAGATTAATGGCTGTGACATCGGCGTGAGAGCTCTTGCATCCCATCCCATGAAAGCCAACAAGAAAGGGATTGGAGAGAAACAGGTACCGATAACCATTGCCGGGACAAGAATCTGTGACGGGGAATGGCTTTATGCAGACACCGATGGAATTCTGATCTCTCGTACCGAATTATCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.69

Weight (kDa)

5.69

Isoelectric Point (pI)

24.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 1.7e-44 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 232
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 315
AciI CCGC 1 cut(s) 226
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 405, 484
AfiI CCNNNNNNNGG 3 cut(s) 106, 205, 420
AgsI TTSAA 1 cut(s) 146
AluBI AGCT 2 cut(s) 263, 350
AluI AGCT 2 cut(s) 263, 350
Alw21I GWGCWC 1 cut(s) 352
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 2 cut(s) 3, 97
ApoI RAATTY 1 cut(s) 468
ArsI GACNNNNNNTTYG 1 cut(s) 480
AseI ATTAAT 1 cut(s) 324
Asp718I GGTACC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 173
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 352
BccI CCATC 4 cut(s) 203, 270, 369, 458
BceAI ACGGC 1 cut(s) 205
BcnI CCSGG 1 cut(s) 421
BfmI CTRYAG 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 421
BmsI GCATC 1 cut(s) 365
BpiI GAAGAC 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 6
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 205, 420
Bse118I RCCGGY 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 4 cut(s) 214, 281, 356, 361
BseLI CCNNNNNNNGG 3 cut(s) 106, 205, 420
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 191
BshFI GGCC 2 cut(s) 5, 99
BshNI GGYRCC 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 352
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 100, 420
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 3 cut(s) 106, 205, 420
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 2 cut(s) 5, 99
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 2 cut(s) 61, 474
BspACI CCGC 1 cut(s) 226
BspANI GGCC 2 cut(s) 5, 99
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 414
BsrFI RCCGGY 1 cut(s) 99
BssAI RCCGGY 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 2 cut(s) 61, 474
BssT1I CCWWGG 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 228
BstC8I GCNNGC 3 cut(s) 81, 101, 224
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 4 cut(s) 214, 281, 356, 361
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 2 cut(s) 64, 477
BstMBI GATC 2 cut(s) 61, 474
BstMWI GCNNNNNNNGC 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 252
BstV2I GAAGAC 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 95
BsuRI GGCC 2 cut(s) 5, 99
BtsCI GGATG 4 cut(s) 214, 281, 356, 361
Cac8I GCNNGC 3 cut(s) 81, 101, 224
CfoI GCGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 99
CseI GACGC 1 cut(s) 41
Csp6I GTAC 2 cut(s) 404, 483
CviAII CATG 1 cut(s) 367
CviJI RGCY 9 cut(s) 5, 79, 99, 263, 300, 330, 350, 374, 448
CviKI_1 RGCY 9 cut(s) 5, 79, 99, 263, 300, 330, 350, 374, 448
CviQI GTAC 2 cut(s) 404, 483
DdeI CTNAG 1 cut(s) 264
DpnI GATC 2 cut(s) 63, 476
DpnII GATC 2 cut(s) 61, 474
EaeI YGGCCR 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 1 cut(s) 370
FaiI YATR 5 cut(s) 94, 96, 305, 368, 453
FaqI GGGAC 1 cut(s) 436
FatI CATG 1 cut(s) 366
FblI GTMKAC 1 cut(s) 315
FokI GGATG 4 cut(s) 221, 288, 343, 348
FriOI GRGCYC 1 cut(s) 352
FspI TGCGCA 1 cut(s) 232
GlaI GCGC 1 cut(s) 232
HaeIII GGCC 2 cut(s) 5, 99
HapII CCGG 2 cut(s) 100, 420
HgaI GACGC 1 cut(s) 41
HhaI GCGC 1 cut(s) 233
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 2 cut(s) 195, 429
HpaII CCGG 2 cut(s) 100, 420
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 2 cut(s) 267, 474
Hpy188III TCNNGA 3 cut(s) 88, 117, 173
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 175
HpyCH4V TGCA 2 cut(s) 356, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 228
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 1 cut(s) 231
KpnI GGTACC 1 cut(s) 407
KroI GCCGGC 1 cut(s) 99
KroNI GCCGGC 1 cut(s) 101
Kzo9I GATC 2 cut(s) 61, 474
LpnPI CCDG 9 cut(s) 92, 93, 101, 102, 113, 264, 267, 386, 433
LweI GCATC 1 cut(s) 365
MaeIII GTNAC 5 cut(s) 10, 29, 127, 332, 434
MalI GATC 2 cut(s) 63, 476
MboI GATC 2 cut(s) 61, 474
MboII GAAGA 2 cut(s) 56, 158
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 2 cut(s) 468, 488
MlyI GAGTC 1 cut(s) 204
MnlI CCTC 2 cut(s) 169, 236
MroNI GCCGGC 1 cut(s) 99
MseI TTAA 2 cut(s) 134, 324
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 2 cut(s) 100, 420
MspR9I CCNGG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 228
NaeI GCCGGC 1 cut(s) 101
NciI CCSGG 1 cut(s) 421
NdeII GATC 2 cut(s) 61, 474
NgoMIV GCCGGC 1 cut(s) 99
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 3 cut(s) 29, 332, 434
NsbI TGCGCA 1 cut(s) 232
PaeR7I CTCGAG 1 cut(s) 173
PctI GAATGC 1 cut(s) 83
PdiI GCCGGC 1 cut(s) 101
PfeI GAWTC 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 152
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 352
PspN4I GGNNCC 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 2 cut(s) 405, 484
RsaNI GTAC 2 cut(s) 404, 483
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 352
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 2 cut(s) 134, 324
Sau3AI GATC 2 cut(s) 61, 474
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 352
SetI ASST 6 cut(s) 111, 134, 141, 265, 352, 405
SfaNI GCATC 1 cut(s) 365
SfcI CTRYAG 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 74, 173
SmoI CTYRAG 2 cut(s) 74, 173
Sse9I AATT 2 cut(s) 468, 488
SsiI CCGC 1 cut(s) 226
SspI AATATT 1 cut(s) 237
SstI GAGCTC 1 cut(s) 352
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 1 cut(s) 6
TaqI TCGA 2 cut(s) 174, 315
TasI AATT 2 cut(s) 468, 488
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 134, 324
Tru9I TTAA 2 cut(s) 134, 324
TseFI GTSAC 3 cut(s) 29, 332, 434
Tsp45I GTSAC 3 cut(s) 29, 332, 434
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 288
Tth111I GACNNNGTC 1 cut(s) 152
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 1 cut(s) 468
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.