MD12G1086900.v1.1

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011677
Physical Location & Seq
Forward (+)
10657699 .. 10659941
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1086900.v1.1.491

Sequence Viewer

Length: 501 bp
ATGGCCTTGGTTACAACTGCTGAAGTTTGCGATGCGCACTCTCAGTTGATTGGGAGTGGTGATCTTCGGGTGCTTGAGCCAATATTCCAGATATATGGCCGGCGACAGGTCTTCTCTGGACAAATAGTTACCTTAAAGGTGTTTGAAGACAATGTCCTCGTTCGTTCCTTTCTCGAGGAGAAAGGCAATGGAAGAGTTCTTGTTGTGGACGGAGGTGGAAGCAAGCGGTGTGCAATATTGGGAGGCAACCCTGTGGTTCAAGCTCAGAATAACGGCTGGGCAGGTATAGTGGTCAATGGCTGCATAAGAGATGTCGACGAGATTAACGGTTGCGACATTGGCGTGAGAGCTCTGGCGGCCCATCCCATGAAAGCCAACAAGAAAGGGATCGGCGAGAAGCAGGTACCAATAAGCATTGCCGGGACAAGGATCTGTGACGGGGAATGGCTTTATGCAGACACCGATGGAATATTGATTTCTCGTACCGAGTTATCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.73

Weight (kDa)

5.69

Isoelectric Point (pI)

26.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 5.7e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 36
Acc36I ACCTGC 2 cut(s) 272, 391
Acc65I GGTACC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 315
AciI CCGC 2 cut(s) 226, 356
AclWI GGATC 2 cut(s) 395, 437
AcoI YGGCCR 1 cut(s) 97
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 2 cut(s) 405, 484
AfiI CCNNNNNNNGG 2 cut(s) 106, 426
AgsI TTSAA 2 cut(s) 146, 260
AluBI AGCT 2 cut(s) 263, 350
AluI AGCT 2 cut(s) 263, 350
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 2 cut(s) 395, 437
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 3 cut(s) 3, 97, 357
ApeKI GCWGC 1 cut(s) 300
Asp718I GGTACC 1 cut(s) 403
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 71
AvaI CYCGRG 1 cut(s) 173
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 2 cut(s) 103, 153
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 1 cut(s) 287
BccI CCATC 2 cut(s) 369, 458
BceAI ACGGC 1 cut(s) 289
BcnI CCSGG 1 cut(s) 421
BfuAI ACCTGC 2 cut(s) 272, 391
BisI GCNGC 2 cut(s) 301, 357
BlsI GCNGC 2 cut(s) 302, 358
Bme1390I CCNGG 1 cut(s) 421
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 421
BmsI GCATC 1 cut(s) 22
BpiI GAAGAC 2 cut(s) 103, 153
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 6
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 426
Bse118I RCCGGY 1 cut(s) 99
Bse3DI GCAATG 2 cut(s) 193, 414
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 2 cut(s) 106, 426
BseMI GCAATG 2 cut(s) 193, 414
BseMII CTCAG 2 cut(s) 56, 278
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 287
BseYI CCCAGC 1 cut(s) 276
BshFI GGCC 3 cut(s) 5, 99, 359
BshNI GGYRCC 1 cut(s) 403
BsiHKAI GWGCWC 1 cut(s) 352
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 100, 420
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 2 cut(s) 106, 426
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 3 cut(s) 5, 99, 359
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 3 cut(s) 61, 387, 429
BspACI CCGC 2 cut(s) 226, 356
BspANI GGCC 3 cut(s) 5, 99, 359
BspCNI CTCAG 2 cut(s) 55, 277
BspLI GGNNCC 1 cut(s) 405
BspMI ACCTGC 2 cut(s) 272, 391
BspPI GGATC 2 cut(s) 395, 437
BspT107I GGYRCC 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 193, 414
BsrFI RCCGGY 1 cut(s) 99
BssAI RCCGGY 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 3 cut(s) 61, 387, 429
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 329
Bst6I CTCTTC 1 cut(s) 187
BstC8I GCNNGC 2 cut(s) 101, 224
BstDEI CTNAG 2 cut(s) 42, 264
BstF5I GGATG 1 cut(s) 361
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 3 cut(s) 64, 390, 432
BstMBI GATC 3 cut(s) 61, 387, 429
BstMWI GCNNNNNNNGC 2 cut(s) 339, 356
BstSCI CCNGG 1 cut(s) 419
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 2 cut(s) 103, 153
BstX2I RGATCY 1 cut(s) 429
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 1 cut(s) 429
BsuRI GGCC 3 cut(s) 5, 99, 359
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 361
BveI ACCTGC 2 cut(s) 272, 391
Cac8I GCNNGC 2 cut(s) 101, 224
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 99
Cfr13I GGNCC 1 cut(s) 358
Csp6I GTAC 2 cut(s) 404, 483
CviAII CATG 1 cut(s) 367
CviQI GTAC 2 cut(s) 404, 483
DdeI CTNAG 2 cut(s) 42, 264
DpnI GATC 3 cut(s) 63, 389, 431
DpnII GATC 3 cut(s) 61, 387, 429
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 187
EarI CTCTTC 1 cut(s) 187
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 1 cut(s) 370
FaiI YATR 6 cut(s) 94, 96, 287, 305, 368, 453
FaqI GGGAC 1 cut(s) 436
FatI CATG 1 cut(s) 366
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 2 cut(s) 301, 357
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 2 cut(s) 301, 357
FspAI RTGCGCAY 1 cut(s) 36
FspI TGCGCA 1 cut(s) 36
GlaI GCGC 1 cut(s) 36
GluI GCNGC 2 cut(s) 301, 357
GsaI CCCAGC 1 cut(s) 280
HaeIII GGCC 3 cut(s) 5, 99, 359
HapII CCGG 2 cut(s) 100, 420
HhaI GCGC 1 cut(s) 37
Hin1II CATG 1 cut(s) 370
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HpaII CCGG 2 cut(s) 100, 420
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 2 cut(s) 208, 316
Hpy188I TCNGA 2 cut(s) 267, 500
Hpy188III TCNNGA 3 cut(s) 88, 117, 173
Hpy8I GTNNAC 2 cut(s) 208, 316
Hpy99I CGWCG 1 cut(s) 320
HpyCH4III ACNGT 1 cut(s) 329
HpyCH4V TGCA 3 cut(s) 233, 303, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 356
HpyF3I CTNAG 2 cut(s) 42, 264
Hsp92II CATG 1 cut(s) 370
HspAI GCGC 1 cut(s) 35
KpnI GGTACC 1 cut(s) 407
KroI GCCGGC 1 cut(s) 99
KroNI GCCGGC 1 cut(s) 101
Kzo9I GATC 3 cut(s) 61, 387, 429
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 22
MaeIII GTNAC 3 cut(s) 10, 127, 434
MalI GATC 3 cut(s) 63, 389, 431
MboI GATC 3 cut(s) 61, 387, 429
MboII GAAGA 4 cut(s) 56, 103, 158, 204
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 352
MnlI CCTC 4 cut(s) 167, 169, 206, 236
MroNI GCCGGC 1 cut(s) 99
MseI TTAA 2 cut(s) 134, 324
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 2 cut(s) 100, 420
MspR9I CCNGG 1 cut(s) 421
MwoI GCNNNNNNNGC 2 cut(s) 339, 356
NaeI GCCGGC 1 cut(s) 101
NciI CCSGG 1 cut(s) 421
NdeII GATC 3 cut(s) 61, 387, 429
NgoMIV GCCGGC 1 cut(s) 99
NlaIII CATG 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 1 cut(s) 434
NsbI TGCGCA 1 cut(s) 36
PaeR7I CTCGAG 1 cut(s) 173
PdiI GCCGGC 1 cut(s) 101
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 2 cut(s) 302, 358
Psp124BI GAGCTC 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 429
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 2 cut(s) 405, 484
RsaNI GTAC 2 cut(s) 404, 483
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 352
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 2 cut(s) 134, 324
SatI GCNGC 2 cut(s) 301, 357
Sau3AI GATC 3 cut(s) 61, 387, 429
Sau96I GGNCC 1 cut(s) 358
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 352
SetI ASST 8 cut(s) 111, 134, 141, 217, 265, 286, 352, 405
SfaNI GCATC 1 cut(s) 22
Sfr274I CTCGAG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 74, 173
SmoI CTYRAG 2 cut(s) 74, 173
SsiI CCGC 2 cut(s) 226, 356
SspI AATATT 3 cut(s) 84, 237, 471
SstI GAGCTC 1 cut(s) 352
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 329
TaqI TCGA 2 cut(s) 174, 315
TauI GCSGC 1 cut(s) 359
Tru1I TTAA 2 cut(s) 134, 324
Tru9I TTAA 2 cut(s) 134, 324
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 225
Tth111I GACNNNGTC 1 cut(s) 152
XhoI CTCGAG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.