Rroxscaffold_6G00426060

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011677
Physical Location & Seq
Forward (+)
46945260 .. 46946081
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00426060.1

Sequence Viewer

Length: 543 bp
ATGGCCTTGGTTACAACTGCTGAAGTCTGCGATGCACATTCGTCGCTCATTGTAAGTGGTGAGCTTCGGGTGCTTGAGCCGATCTTTCAGGTATATGGGCGGCGACAGGTCTTCTCTGGACAAGTAGTTACCTTAAAGGTATTCGAAGACAATGTTCTGGTTCGTGCCTTTCTTGAGGAGAAAGGCAATGGCAGAGTTCTTGTTGTGGACGGGGGTGGAAGCAAGCGGTGTGCAATATTGGGTGGCAACCCTGTAGTTCAAGCCCAGAATAATGGCTGGGCTGGTATAGTGGTCAATGGCTGCATAAGAGATGTTGATGAGATTGAAGGCTGTGACATTGGGGTGAGAGCTCTGGCTTCCCATCCAATGAAGGCCAATAAGAAGGGAACCGGAGAGAAGCAAGTACCGATAACCATTGCTGGGACAAGGATCAACGATGGGGAATGGCTTTATGCAGACACCGATGGAATTCTGATTTCTCATACTGAGCTATCTGCAACAGAAAATGTTGCTTTGACATATACCGTGATAGTGCTACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

180

Amino Acids

19.23

Weight (kDa)

5.2

Isoelectric Point (pI)

21.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 1.9e-45 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 100, 226
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 42
AfaI GTAC 1 cut(s) 405
AfiI CCNNNNNNNGG 1 cut(s) 420
AgsI TTSAA 2 cut(s) 260, 326
AluBI AGCT 3 cut(s) 64, 350, 490
AluI AGCT 3 cut(s) 64, 350, 490
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 1 cut(s) 437
AoxI GGCC 2 cut(s) 3, 372
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 468
AsuHPI GGTGA 2 cut(s) 71, 355
AsuII TTCGAA 1 cut(s) 144
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 2 cut(s) 103, 153
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 1 cut(s) 287
BccI CCATC 3 cut(s) 369, 431, 458
BcgI CGANNNNNNTGC 2 cut(s) 24, 58
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 2 cut(s) 101, 301
BlsI GCNGC 2 cut(s) 102, 302
BmiI GGNNCC 1 cut(s) 388
BmsI GCATC 1 cut(s) 22
BpiI GAAGAC 2 cut(s) 103, 153
Bpu14I TTCGAA 1 cut(s) 144
BpuEI CTTGAG 2 cut(s) 95, 194
BsaJI CCNNGG 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 1 cut(s) 420
Bse3DI GCAATG 2 cut(s) 193, 414
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 420
BseMI GCAATG 2 cut(s) 193, 414
BseMII CTCAG 1 cut(s) 477
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 287
BseYI CCCAGC 2 cut(s) 276, 419
BshFI GGCC 2 cut(s) 5, 374
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 1 cut(s) 390
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 2 cut(s) 5, 374
Bsp119I TTCGAA 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 2 cut(s) 81, 429
BspACI CCGC 2 cut(s) 100, 226
BspANI GGCC 2 cut(s) 5, 374
BspCNI CTCAG 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 388
BspPI GGATC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 144
BsrDI GCAATG 2 cut(s) 193, 414
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 2 cut(s) 81, 429
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 486
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 2 cut(s) 84, 432
BstMBI GATC 2 cut(s) 81, 429
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 2 cut(s) 103, 153
BstXI CCANNNNNNTGG 1 cut(s) 272
BsuRI GGCC 2 cut(s) 5, 374
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 404
CviQI GTAC 1 cut(s) 404
DdeI CTNAG 1 cut(s) 486
DpnI GATC 2 cut(s) 83, 431
DpnII GATC 2 cut(s) 81, 429
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 6
FaiI YATR 9 cut(s) 94, 96, 287, 305, 453, 483, 520, 522, 541
FaqI GGGAC 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 101, 301
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 2 cut(s) 101, 301
GluI GCNGC 2 cut(s) 101, 301
GsaI CCCAGC 2 cut(s) 280, 423
HaeIII GGCC 2 cut(s) 5, 374
HapII CCGG 1 cut(s) 390
HpaII CCGG 1 cut(s) 390
HphI GGTGA 2 cut(s) 71, 355
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 2 cut(s) 117, 173
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 320, 364, 376
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4V TGCA 5 cut(s) 35, 233, 303, 455, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 1 cut(s) 486
Kzo9I GATC 2 cut(s) 81, 429
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 22
MaeIII GTNAC 3 cut(s) 10, 127, 332
MalI GATC 2 cut(s) 83, 431
MboI GATC 2 cut(s) 81, 429
MboII GAAGA 2 cut(s) 103, 158
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 1 cut(s) 468
MnlI CCTC 1 cut(s) 169
MseI TTAA 1 cut(s) 134
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 2 cut(s) 81, 429
NlaIV GGNNCC 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 332
NspV TTCGAA 1 cut(s) 144
PkrI GCNGC 2 cut(s) 102, 302
Psp124BI GAGCTC 1 cut(s) 352
PspFI CCCAGC 2 cut(s) 276, 419
PspN4I GGNNCC 1 cut(s) 388
RsaI GTAC 1 cut(s) 405
RsaNI GTAC 1 cut(s) 404
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 2 cut(s) 101, 301
Sau3AI GATC 2 cut(s) 81, 429
SduI GDGCHC 1 cut(s) 352
SetI ASST 7 cut(s) 66, 93, 111, 134, 141, 352, 492
SfaNI GCATC 1 cut(s) 22
SfcI CTRYAG 1 cut(s) 252
SfuI TTCGAA 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 74, 173
SmoI CTYRAG 2 cut(s) 74, 173
Sse9I AATT 1 cut(s) 468
SsiI CCGC 2 cut(s) 100, 226
SspI AATATT 1 cut(s) 237
SstI GAGCTC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 526
TaqI TCGA 1 cut(s) 144
TasI AATT 1 cut(s) 468
TauI GCSGC 1 cut(s) 103
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 383
XapI RAATTY 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.