AT5G16450

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011677
Physical Location & Seq
Forward (+)
5373820 .. 5375459
1640 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16450.1

Sequence Viewer

Length: 501 bp
ATGGCGTTTGTGACAACTGCGGAAGTTTGTGACGCGAATCAAGAGCTGATTCGGAGTGGTCAGCTACGAGCTTTGCAACCCATTTTCCAGATTTATGGCCGTCGACAAATATTCTCAGGACCAGTTGTCACTGTCAAAGTTTTTGAAGACAATGGCTTAATCCGTCAATTCATCGAGGAGAAAGGAAACGGTAGAGTTCTTGTGGTGGATGGTGGAGGGAGTCAACGATGTGCAATACTCGGAGGTAACCCCGTTGTTCAAGCTCAGAACAACGGATGGGCAGGAATCGTTGTGAACGGATGCATCAGAGACGTCGATGAGATCAACGGTTGTGATATCGGAGTGAGAGCTTTGGCCTCTCATCCGATAAAAGCGAGTAAGAAAGGACTTGGAGAACAACGAGTTCCGGTAAATATCGCAGGGACTCGGATTTGTGATGGTGAATGGCTTTATGCAGATACTGATGGGATTCTTGTCTCCCAAATTGAGTTATCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

166

Amino Acids

17.82

Weight (kDa)

5.42

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 6 - 159 4.8e-47 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
AccI GTMKAC 1 cut(s) 103
AccII CGCG 1 cut(s) 35
AciI CCGC 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 97
AcyI GRCGYC 1 cut(s) 312
AgsI TTSAA 2 cut(s) 146, 260
AhdI GACNNNNNGTC 1 cut(s) 125
AluBI AGCT 5 cut(s) 46, 64, 71, 263, 350
AluI AGCT 5 cut(s) 46, 64, 71, 263, 350
Alw26I GTCTC 2 cut(s) 303, 481
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 2 cut(s) 97, 354
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 1 cut(s) 452
AvaII GGWCC 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 153
BccI CCATC 4 cut(s) 203, 270, 431, 458
BceAI ACGGC 1 cut(s) 84
BcoDI GTCTC 2 cut(s) 303, 481
Bme18I GGWCC 1 cut(s) 119
BmeRI GACNNNNNGTC 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 119
BmsI GCATC 2 cut(s) 290, 312
BpiI GAAGAC 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 312
BsaWI WCCGGW 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 122
BseGI GGATG 4 cut(s) 214, 281, 305, 361
BseMII CTCAG 2 cut(s) 129, 278
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 191
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 99, 356
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 436
BsmAI GTCTC 2 cut(s) 303, 481
BsmBI CGTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 2 cut(s) 99, 356
Bsp143I GATC 1 cut(s) 321
BspACI CCGC 1 cut(s) 20
BspANI GGCC 2 cut(s) 99, 356
BspCNI CTCAG 2 cut(s) 128, 277
BspFNI CGCG 1 cut(s) 35
BsrI ACTGG 1 cut(s) 122
BssMI GATC 1 cut(s) 321
BssNI GRCGYC 1 cut(s) 312
Bst4CI ACNGT 3 cut(s) 133, 191, 329
BstACI GRCGYC 1 cut(s) 312
BstDEI CTNAG 2 cut(s) 115, 264
BstEII GGTNACC 1 cut(s) 245
BstF5I GGATG 4 cut(s) 214, 281, 305, 361
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 1 cut(s) 324
BstMAI GTCTC 2 cut(s) 303, 481
BstMBI GATC 1 cut(s) 321
BstPI GGTNACC 1 cut(s) 245
BstUI CGCG 1 cut(s) 35
BstV2I GAAGAC 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 95
BsuRI GGCC 2 cut(s) 99, 356
BtsCI GGATG 4 cut(s) 214, 281, 305, 361
BtsIMutI CAGTG 1 cut(s) 129
CaiI CAGNNNCTG 1 cut(s) 461
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 1 cut(s) 41
CviJI RGCY 9 cut(s) 46, 64, 71, 99, 156, 263, 350, 356, 448
CviKI_1 RGCY 9 cut(s) 46, 64, 71, 99, 156, 263, 350, 356, 448
DdeI CTNAG 2 cut(s) 115, 264
DpnI GATC 1 cut(s) 323
DpnII GATC 1 cut(s) 321
DriI GACNNNNNGTC 1 cut(s) 125
EaeI YGGCCR 1 cut(s) 97
Eam1105I GACNNNNNGTC 1 cut(s) 125
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 245
EcoRV GATATC 1 cut(s) 337
EcoT22I ATGCAT 1 cut(s) 305
Esp3I CGTCTC 1 cut(s) 303
FaiI YATR 2 cut(s) 96, 453
FaqI GGGAC 1 cut(s) 436
FblI GTMKAC 1 cut(s) 103
FokI GGATG 4 cut(s) 221, 288, 312, 348
HaeIII GGCC 2 cut(s) 99, 356
HapII CCGG 1 cut(s) 407
HgaI GACGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 312
HincII GTYRAC 2 cut(s) 104, 224
HindII GTYRAC 2 cut(s) 104, 224
HinfI GANTC 6 cut(s) 37, 49, 220, 285, 424, 469
HpaII CCGG 1 cut(s) 407
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 3 cut(s) 104, 224, 295
Hpy188I TCNGA 7 cut(s) 54, 242, 267, 308, 341, 366, 429
Hpy188III TCNNGA 3 cut(s) 41, 88, 117
Hpy8I GTNNAC 3 cut(s) 104, 224, 295
Hpy99I CGWCG 2 cut(s) 105, 317
HpyCH4III ACNGT 3 cut(s) 133, 191, 329
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 4 cut(s) 76, 233, 303, 455
HpyF3I CTNAG 2 cut(s) 115, 264
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 312
Kzo9I GATC 1 cut(s) 321
LpnPI CCDG 6 cut(s) 101, 102, 135, 267, 405, 420
LweI GCATC 2 cut(s) 290, 312
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 4 cut(s) 10, 29, 127, 245
MalI GATC 1 cut(s) 323
MboI GATC 1 cut(s) 321
MboII GAAGA 1 cut(s) 158
MluCI AATT 2 cut(s) 167, 483
MlyI GAGTC 2 cut(s) 229, 418
MnlI CCTC 4 cut(s) 169, 209, 236, 367
Mph1103I ATGCAT 1 cut(s) 305
MseI TTAA 2 cut(s) 158, 499
MspI CCGG 1 cut(s) 407
MvnI CGCG 1 cut(s) 35
NdeII GATC 1 cut(s) 321
NmuCI GTSAC 3 cut(s) 10, 29, 127
NsiI ATGCAT 1 cut(s) 305
PfeI GAWTC 4 cut(s) 37, 49, 285, 469
PleI GAGTC 2 cut(s) 228, 418
PpsI GAGTC 2 cut(s) 228, 418
PspEI GGTNACC 1 cut(s) 245
PspPI GGNCC 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 461
SalI GTCGAC 1 cut(s) 102
SaqAI TTAA 2 cut(s) 158, 499
Sau3AI GATC 1 cut(s) 321
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 2 cut(s) 229, 418
SetI ASST 7 cut(s) 48, 66, 73, 247, 265, 315, 352
SfaNI GCATC 2 cut(s) 290, 312
SinI GGWCC 1 cut(s) 119
Sse9I AATT 2 cut(s) 167, 483
SsiI CCGC 1 cut(s) 20
SspI AATATT 1 cut(s) 111
TaaI ACNGT 3 cut(s) 133, 191, 329
TaiI ACGT 1 cut(s) 315
TaqI TCGA 3 cut(s) 103, 174, 315
TasI AATT 2 cut(s) 167, 483
TfiI GAWTC 4 cut(s) 37, 49, 285, 469
Tru1I TTAA 2 cut(s) 158, 499
Tru9I TTAA 2 cut(s) 158, 499
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 3 cut(s) 10, 29, 127
Tsp45I GTSAC 3 cut(s) 10, 29, 127
TspDTI ATGAA 1 cut(s) 160
TspGWI ACGGA 4 cut(s) 152, 288, 312, 484
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 103
ZraI GACGTC 1 cut(s) 313
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.