FvH4_6g00480

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2- oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011677
Physical Location & Seq
Forward (+)
307044 .. 309484
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g00480.t2

Sequence Viewer

Length: 501 bp
ATGGCCTTGGTTACAACTGCTGAAGTCTGCGATGCACACTCATCGCTCATTGTAAGTGGTGAGCTTCGGGTGCTTGAGCCGATCTTCCAGATATATGGGCGGCGACCGGTCTTCTCTGGACCAGTAGTTACCCTGAAGGTATTTGAAGACAATGTTCTGGTTCGTGGGTTTCTTGAGGAGAAAGGCAATGGCAGAGTTCTTGTTGTGGACGGGGGTGGAAGCAAGCGGTGTGCAATATTAGGTGGCAACCCTGTAGTTCAAGCTCAGAATAATGGCTGGGCTGGTATAGTGGTCAATGGCTGCATAAGAGATGTTGATGAGATTAATGGCTGTGACATTGGGGTGAGAGCTCTGGCTTCTCATCCAATGAAAGCCAACAAGAAGGGAACAGGAGAGAAGCAAGTACCAATAACCATAGCTGGGACAAGGATCACTGATGGGGAATGGCTTTATGCAGACACCGATGGAATTCTAATTTCTCATACTGAGCTATCTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RraA-like

Protein Analysis

167

Amino Acids

17.64

Weight (kDa)

5.6

Isoelectric Point (pI)

22.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RraA-like PF03737 41 - 194 1.1e-44 Aldolase/RraA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 100, 226
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 2 cut(s) 42, 155
AfaI GTAC 1 cut(s) 405
AfiI CCNNNNNNNGG 1 cut(s) 420
AgeI ACCGGT 1 cut(s) 106
AgsI TTSAA 2 cut(s) 146, 260
AluBI AGCT 5 cut(s) 64, 263, 350, 419, 490
AluI AGCT 5 cut(s) 64, 263, 350, 419, 490
Alw21I GWGCWC 1 cut(s) 352
AlwI GGATC 1 cut(s) 437
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 468
AseI ATTAAT 1 cut(s) 324
AsiGI ACCGGT 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 71, 355
AvaII GGWCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 2 cut(s) 103, 153
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 1 cut(s) 287
BccI CCATC 2 cut(s) 431, 458
BcgI CGANNNNNNTGC 2 cut(s) 24, 58
BfmI CTRYAG 1 cut(s) 252
BisI GCNGC 2 cut(s) 101, 301
BlsI GCNGC 2 cut(s) 102, 302
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmsI GCATC 1 cut(s) 22
BpiI GAAGAC 2 cut(s) 103, 153
BpuEI CTTGAG 2 cut(s) 95, 194
BsaJI CCNNGG 1 cut(s) 6
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 420
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 193
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 420
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 2 cut(s) 278, 477
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 1 cut(s) 287
BseYI CCCAGC 2 cut(s) 276, 419
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 5
BshTI ACCGGT 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 352
BsiSI CCGG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 352
Bsp143I GATC 2 cut(s) 81, 429
BspACI CCGC 2 cut(s) 100, 226
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 277, 478
BspPI GGATC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 106
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 2 cut(s) 81, 429
BssT1I CCWWGG 1 cut(s) 6
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 2 cut(s) 264, 486
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 2 cut(s) 84, 432
BstMBI GATC 2 cut(s) 81, 429
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 252
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 2 cut(s) 103, 153
BstXI CCANNNNNNTGG 1 cut(s) 95
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 2 cut(s) 27, 45
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 404
CspAI ACCGGT 1 cut(s) 106
CviQI GTAC 1 cut(s) 404
DdeI CTNAG 2 cut(s) 264, 486
DpnI GATC 2 cut(s) 83, 431
DpnII GATC 2 cut(s) 81, 429
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 352
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 2 cut(s) 42, 155
EcoICRI GAGCTC 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 6
FaiI YATR 7 cut(s) 94, 96, 287, 305, 416, 453, 483
FaqI GGGAC 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 101, 301
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 352
Fsp4HI GCNGC 2 cut(s) 101, 301
GluI GCNGC 2 cut(s) 101, 301
GsaI CCCAGC 2 cut(s) 280, 423
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 107
HpaII CCGG 1 cut(s) 107
HphI GGTGA 2 cut(s) 71, 355
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 3 cut(s) 88, 117, 173
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 2 cut(s) 130, 376
HpyCH4V TGCA 4 cut(s) 35, 233, 303, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 70
HpyF3I CTNAG 2 cut(s) 264, 486
Kzo9I GATC 2 cut(s) 81, 429
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 22
MaeIII GTNAC 3 cut(s) 10, 127, 332
MalI GATC 2 cut(s) 83, 431
MboI GATC 2 cut(s) 81, 429
MboII GAAGA 3 cut(s) 76, 103, 158
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 2 cut(s) 468, 474
MnlI CCTC 1 cut(s) 169
MseI TTAA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 341
MspI CCGG 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 70
NdeII GATC 2 cut(s) 81, 429
NmuCI GTSAC 1 cut(s) 332
PinAI ACCGGT 1 cut(s) 106
PkrI GCNGC 2 cut(s) 102, 302
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 352
PspFI CCCAGC 2 cut(s) 276, 419
PspPI GGNCC 1 cut(s) 119
RsaI GTAC 1 cut(s) 405
RsaNI GTAC 1 cut(s) 404
RseI CAYNNNNRTG 1 cut(s) 341
SacI GAGCTC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 2 cut(s) 101, 301
Sau3AI GATC 2 cut(s) 81, 429
Sau96I GGNCC 1 cut(s) 119
SduI GDGCHC 1 cut(s) 352
SetI ASST 7 cut(s) 66, 141, 244, 265, 352, 421, 492
SfaNI GCATC 1 cut(s) 22
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 74, 173
SmoI CTYRAG 2 cut(s) 74, 173
Sse9I AATT 2 cut(s) 468, 474
SsiI CCGC 2 cut(s) 100, 226
SspI AATATT 1 cut(s) 237
SstI GAGCTC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 6
TasI AATT 2 cut(s) 468, 474
TauI GCSGC 1 cut(s) 103
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 439
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 383
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 119
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.