AT3G04130

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009286
Physical Location & Seq
Forward (+)
1083772 .. 1086418
2647 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04130.2

Sequence Viewer

Length: 1527 bp
ATGTCTTGGCTTATTCAAAATCGCATTGGAAACACCTTGTTACGCCTTAATCCTTCTTCGTCTTCTATCGCTATTTTCTCTACGTTTATCAAGAATCTCTCTACTGCTTCGGAGCAATTACCTGAGACACTTGATGAATATTCACAGTCTGAGGAGATATGGAATGTTATTGTAGGTAGAGACGGTGATAGAGATAGTGAAGATGATGTTTTTAAACGTCTATCTAGCGATGAAATCTGTAAAAGAGTCAATCTTTCTGATGGTTTGGTTCATAAGCTGCTTCATAGGTTTAGAGATGACTGGAGATCTGCGCTTGGTATTTTGAAATGGGCTGAGTCTTGTAAAGGGCATAAACATTCGAGTGATGCTTATGATATGGCTGTGGATATTCTCGGCAAGGCGAAGAAATGGGATCGGATGAAGGAGTTTGTGGAGAGAATGAGAGGAGATAAACTTGTTACTTTGAATACAGTTGCTAAGATCATGAGGAGATTTGCTGGTGCAGGGGAGTGGGAAGAAGCTGTGGGAATATTTGATAGGTTGGGTGAATTTGGATTGGAGAAGAACACGGAGTCGATGAACTTACTGCTCGATACGCTTTGCAAGGAGAAAAGAGTGGAGCAGGCCCGAGTAGTTTTGCTACAGCTGAAGTCACATATTACACCAAATGCTCATACGTTTAATATCTTCATTCATGGTTGGTGTAAGGCAAATAGAGTTGAGGAAGCTCTTTGGACGATCCAAGAGATGAAAGGTCATGGTTTTCGTCCTTGTGTTATTAGCTATACGACGATTATAAGGTGTTATTGCCAGCAGTTTGAGTTTATTAAGGTCTATGAAATGCTAAGTGAGATGGAAGCTAATGGGTCTCCTCCGAATTCTATTACTTACACAACTATTATGTCTTCTCTTAATGCACAGAAAGAGTTTGAGGAGGCTTTACGGGTTGCTACAAGGATGAAAAGATCCGGCTGTAAACCCGATTCTCTTTTCTACAACTGTTTGATTCACACGCTTGCTCGAGCTGGTCGGTTAGAAGAAGCTGAGCGGGTTTTCAGAGTCGAGATGCCAGAGCTTGGTGTTTCCATTAATACATCTACCTATAACTCTATGATTGCTATGTATTGTCATCACGATGAGGAAGATAAGGCGATTGAGCTTCTCAAGGAAATGGAAAGTTCAAACCTTTGTAATCCCGATGTTCATACGTATCAACCCCTGCTGAGATCTTGTTTTAAGAGAGGAGATGTTGTTGAGGTTGGGAAACTATTGAAAGAGATGGTGACTAAACATCATCTCAGCCTCGATGAGTCGACTTATACTTTTCTGATACAGAGGTTATGCAGAGCTAATATGTGTGAGTGGGCGTATTGTCTATTCGAAGAGATGATTAGCCAAGATATCACGCCGAGGCACCGCACTTGTTTGCTGCTCTTGGAAGAGGTCAAGAAGAAGAATATGCATGAATCAGCTGAGAGAATCGAACACATTATGAAGACTGTGAAACTTACTGCACCTGTAAAGTGA

Protein Analysis

508

Amino Acids

58.7

Weight (kDa)

6.52

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 189 - 236 2.4e-08 PPR repeat family
PPR_1 PF12854 220 - 250 5.4e-08 PPR repeat
PPR_2 PF13041 222 - 271 8.1e-12 PPR repeat family
PPR PF01535 226 - 255 6.2e-06 PPR repeat
PPR_3 PF13812 247 - 302 3.5e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 257 - 304 3.1e-11 PPR repeat family
TPR_24 PF23276 259 - 353 2.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 281 - 338 6e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 295 - 329 3.1e-06 PPR repeat family
PPR_3 PF13812 317 - 375 1.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 324 - 353 1.3e-08 PPR repeat
PPR_2 PF13041 327 - 377 9.6e-11 PPR repeat family
PPR_long PF17177 332 - 444 8.9e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 360 - 392 1.4e-06 PPR repeat
PPR_2 PF13041 364 - 413 4.5e-11 PPR repeat family
PPR PF01535 366 - 395 9.8e-08 PPR repeat
PPR_2 PF13041 399 - 449 7.4e-08 PPR repeat family
PPR_1 PF12854 433 - 463 9.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
AccB1I GGYRCC 1 cut(s) 1413
AccB7I CCANNNNNTGG 1 cut(s) 1076
AccBSI CCGCTC 1 cut(s) 1048
AccI GTMKAC 1 cut(s) 1313
AciI CCGC 2 cut(s) 1048, 1417
AclWI GGATC 3 cut(s) 420, 733, 960
AcsI RAATTY 2 cut(s) 548, 877
AcuI CTGAAG 1 cut(s) 668
AfiI CCNNNNNNNGG 1 cut(s) 1076
AgsI TTSAA 5 cut(s) 17, 325, 466, 1182, 1273
Alw26I GTCTC 3 cut(s) 119, 174, 873
AlwI GGATC 3 cut(s) 420, 733, 960
Ama87I CYCGRG 2 cut(s) 627, 1020
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 2 cut(s) 277, 1429
ApoI RAATTY 2 cut(s) 548, 877
AseI ATTAAT 1 cut(s) 1089
AspLEI GCGC 1 cut(s) 313
AspS9I GGNCC 1 cut(s) 625
AsuHPI GGTGA 3 cut(s) 197, 557, 1294
AsuII TTCGAA 1 cut(s) 1380
AvaI CYCGRG 2 cut(s) 627, 1020
BaeI ACNNNNGTAYC 2 cut(s) 1322, 1355
BanI GGYRCC 1 cut(s) 1413
BbsI GAAGAC 3 cut(s) 54, 897, 1502
BbvI GCAGC 2 cut(s) 264, 1416
BccI CCATC 3 cut(s) 254, 847, 1273
BcoDI GTCTC 3 cut(s) 119, 174, 873
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 641
BglII AGATCT 2 cut(s) 305, 1226
BisI GCNGC 2 cut(s) 278, 1430
BlpI GCTNAGC 1 cut(s) 1044
BlsI GCNGC 2 cut(s) 279, 1431
BmeT110I CYCGRG 2 cut(s) 627, 1020
BmgT120I GGNCC 1 cut(s) 625
BmiI GGNNCC 1 cut(s) 1415
BmsI GCATC 2 cut(s) 355, 1056
BpiI GAAGAC 3 cut(s) 54, 897, 1502
BpmI CTGGAG 1 cut(s) 322
Bpu1102I GCTNAGC 1 cut(s) 1044
Bpu14I TTCGAA 1 cut(s) 1380
BpuEI CTTGAG 1 cut(s) 1148
BsaAI YACGTR 1 cut(s) 1209
BsaI GGTCTC 1 cut(s) 873
BsaJI CCNNGG 1 cut(s) 1409
BsaXI ACNNNNNCTCC 2 cut(s) 1236, 1266
Bsc4I CCNNNNNNNGG 1 cut(s) 1076
Bse1I ACTGG 1 cut(s) 305
BseDI CCNNGG 1 cut(s) 1409
BseGI GGATG 2 cut(s) 423, 963
BseLI CCNNNNNNNGG 1 cut(s) 1076
BseMII CTCAG 7 cut(s) 114, 141, 324, 1035, 1214, 1312, 1464
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 6 cut(s) 167, 459, 502, 861, 947, 1257
BseXI GCAGC 2 cut(s) 264, 1416
BsgI GTGCAG 2 cut(s) 522, 1497
BshFI GGCC 1 cut(s) 626
BshNI GGYRCC 1 cut(s) 1413
BsiHKCI CYCGRG 2 cut(s) 627, 1020
BsiSI CCGG 1 cut(s) 969
BslI CCNNNNNNNGG 1 cut(s) 1076
BsmAI GTCTC 3 cut(s) 119, 174, 873
BsmBI CGTCTC 1 cut(s) 174
BsnI GGCC 1 cut(s) 626
Bso31I GGTCTC 1 cut(s) 873
BsoBI CYCGRG 2 cut(s) 627, 1020
Bsp119I TTCGAA 1 cut(s) 1380
Bsp143I GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
Bsp1720I GCTNAGC 1 cut(s) 1044
BspACI CCGC 2 cut(s) 1048, 1417
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 7 cut(s) 115, 142, 325, 1036, 1215, 1311, 1465
BspHI TCATGA 1 cut(s) 483
BspLI GGNNCC 1 cut(s) 1415
BspPI GGATC 3 cut(s) 420, 733, 960
BspT104I TTCGAA 1 cut(s) 1380
BspT107I GGYRCC 1 cut(s) 1413
BspTNI GGTCTC 1 cut(s) 873
BsrBI CCGCTC 1 cut(s) 1048
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 1409
BssMI GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
Bst4CI ACNGT 5 cut(s) 147, 185, 472, 1001, 1501
Bst6I CTCTTC 2 cut(s) 1377, 1434
BstBAI YACGTR 1 cut(s) 1209
BstBI TTCGAA 1 cut(s) 1380
BstC8I GCNNGC 3 cut(s) 624, 812, 1017
BstDEI CTNAG 9 cut(s) 123, 150, 333, 477, 845, 1044, 1223, 1298, 1473
BstF5I GGATG 2 cut(s) 423, 963
BstHHI GCGC 1 cut(s) 313
BstKTI GATC 6 cut(s) 308, 415, 483, 741, 968, 1229
BstMAI GTCTC 3 cut(s) 119, 174, 873
BstMBI GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
BstMWI GCNNNNNNNGC 1 cut(s) 595
BstSFI CTRYAG 1 cut(s) 641
BstSNI TACGTA 1 cut(s) 1209
BstV1I GCAGC 2 cut(s) 264, 1416
BstV2I GAAGAC 3 cut(s) 54, 897, 1502
BstX2I RGATCY 3 cut(s) 305, 965, 1226
BstYI RGATCY 3 cut(s) 305, 965, 1226
BsuRI GGCC 1 cut(s) 626
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 2 cut(s) 423, 963
Cac8I GCNNGC 3 cut(s) 624, 812, 1017
CciI TCATGA 1 cut(s) 483
CfoI GCGC 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 625
CviAII CATG 4 cut(s) 484, 695, 758, 1463
DdeI CTNAG 9 cut(s) 123, 150, 333, 477, 845, 1044, 1223, 1298, 1473
DpnI GATC 6 cut(s) 307, 414, 482, 740, 967, 1228
DpnII GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
DraI TTTAAA 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 1377, 1434
EarI CTCTTC 2 cut(s) 1377, 1434
Eco105I TACGTA 1 cut(s) 1209
Eco31I GGTCTC 1 cut(s) 873
Eco32I GATATC 1 cut(s) 1402
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 2 cut(s) 627, 1020
EcoRI GAATTC 1 cut(s) 877
EcoRV GATATC 1 cut(s) 1402
EcoT22I ATGCAT 1 cut(s) 1464
Esp3I CGTCTC 1 cut(s) 174
FaeI CATG 4 cut(s) 487, 698, 761, 1466
FatI CATG 4 cut(s) 483, 694, 757, 1462
FauI CCCGC 1 cut(s) 1041
FblI GTMKAC 1 cut(s) 1313
Fnu4HI GCNGC 2 cut(s) 278, 1430
FokI GGATG 2 cut(s) 430, 970
Fsp4HI GCNGC 2 cut(s) 278, 1430
FspBI CTAG 1 cut(s) 225
GlaI GCGC 1 cut(s) 312
GluI GCNGC 2 cut(s) 278, 1430
GsuI CTGGAG 1 cut(s) 322
HaeIII GGCC 1 cut(s) 626
HapII CCGG 1 cut(s) 969
HhaI GCGC 1 cut(s) 313
Hin1II CATG 4 cut(s) 487, 698, 761, 1466
Hin6I GCGC 1 cut(s) 311
HinP1I GCGC 1 cut(s) 311
HincII GTYRAC 1 cut(s) 1314
HindII GTYRAC 1 cut(s) 1314
HpaII CCGG 1 cut(s) 969
HphI GGTGA 3 cut(s) 197, 557, 1294
Hpy166II GTNNAC 2 cut(s) 977, 1314
Hpy188I TCNGA 7 cut(s) 112, 151, 259, 417, 876, 1058, 1329
Hpy188III TCNNGA 6 cut(s) 91, 484, 1063, 1133, 1196, 1447
Hpy8I GTNNAC 2 cut(s) 977, 1314
Hpy99I CGWCG 1 cut(s) 793
HpyAV CCTTC 2 cut(s) 63, 415
HpyCH4III ACNGT 5 cut(s) 147, 185, 472, 1001, 1501
HpyCH4IV ACGT 4 cut(s) 83, 217, 677, 1208
HpyCH4V TGCA 6 cut(s) 503, 603, 917, 1344, 1462, 1514
HpyF10VI GCNNNNNNNGC 1 cut(s) 595
HpyF3I CTNAG 9 cut(s) 123, 150, 333, 477, 845, 1044, 1223, 1298, 1473
HpySE526I ACGT 4 cut(s) 83, 217, 677, 1208
Hsp92II CATG 4 cut(s) 487, 698, 761, 1466
HspAI GCGC 1 cut(s) 311
Kzo9I GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
LmnI GCTCC 2 cut(s) 112, 619
Lsp1109I GCAGC 2 cut(s) 264, 1416
LweI GCATC 2 cut(s) 355, 1056
MaeI CTAG 1 cut(s) 225
MaeII ACGT 4 cut(s) 83, 217, 677, 1208
MaeIII GTNAC 4 cut(s) 39, 457, 651, 1282
MalI GATC 6 cut(s) 307, 414, 482, 740, 967, 1228
MbiI CCGCTC 1 cut(s) 1048
MboI GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
MflI RGATCY 3 cut(s) 305, 965, 1226
MluCI AATT 3 cut(s) 116, 548, 877
MlyI GAGTC 5 cut(s) 255, 344, 581, 1068, 1319
Mph1103I ATGCAT 1 cut(s) 1464
MseI TTAA 7 cut(s) 48, 213, 681, 828, 912, 1089, 1236
MslI CAYNNNNRTG 2 cut(s) 360, 1134
MspA1I CMGCKG 2 cut(s) 646, 1472
MspI CCGG 1 cut(s) 969
MwoI GCNNNNNNNGC 1 cut(s) 595
NdeII GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
NlaIII CATG 4 cut(s) 487, 698, 761, 1466
NlaIV GGNNCC 1 cut(s) 1415
NmeAIII GCCGAG 2 cut(s) 372, 1434
NmuCI GTSAC 2 cut(s) 651, 1282
NsiI ATGCAT 1 cut(s) 1464
NspV TTCGAA 1 cut(s) 1380
PaeR7I CTCGAG 1 cut(s) 1020
PagI TCATGA 1 cut(s) 483
PfeI GAWTC 5 cut(s) 94, 983, 1006, 1466, 1479
PflMI CCANNNNNTGG 1 cut(s) 1076
PkrI GCNGC 2 cut(s) 279, 1431
PleI GAGTC 5 cut(s) 254, 343, 580, 1067, 1318
PpsI GAGTC 5 cut(s) 254, 343, 580, 1067, 1318
Ppu21I YACGTR 1 cut(s) 1209
PshBI ATTAAT 1 cut(s) 1089
PsiI TTATAA 1 cut(s) 797
PspN4I GGNNCC 1 cut(s) 1415
PspPI GGNCC 1 cut(s) 625
PspXI VCTCGAGB 1 cut(s) 1020
PsuI RGATCY 3 cut(s) 305, 965, 1226
PvuII CAGCTG 2 cut(s) 646, 1472
RseI CAYNNNNRTG 2 cut(s) 360, 1134
SalI GTCGAC 1 cut(s) 1312
SaqAI TTAA 7 cut(s) 48, 213, 681, 828, 912, 1089, 1236
SatI GCNGC 2 cut(s) 278, 1430
Sau3AI GATC 6 cut(s) 305, 412, 480, 738, 965, 1226
Sau96I GGNCC 1 cut(s) 625
SchI GAGTC 5 cut(s) 255, 344, 581, 1068, 1319
SfaNI GCATC 2 cut(s) 355, 1056
SfcI CTRYAG 1 cut(s) 641
Sfr274I CTCGAG 1 cut(s) 1020
SfuI TTCGAA 1 cut(s) 1380
SlaI CTCGAG 1 cut(s) 1020
SmiMI CAYNNNNRTG 2 cut(s) 360, 1134
SmlI CTYRAG 2 cut(s) 1020, 1163
SmoI CTYRAG 2 cut(s) 1020, 1163
SnaBI TACGTA 1 cut(s) 1209
Sse9I AATT 3 cut(s) 116, 548, 877
SsiI CCGC 2 cut(s) 1048, 1417
SspI AATATT 2 cut(s) 140, 531
SspMI CTAG 1 cut(s) 225
TaaI ACNGT 5 cut(s) 147, 185, 472, 1001, 1501
TaiI ACGT 4 cut(s) 86, 220, 680, 1211
TaqI TCGA 9 cut(s) 359, 575, 591, 1021, 1062, 1305, 1313, 1380, 1482
TasI AATT 3 cut(s) 116, 548, 877
TfiI GAWTC 5 cut(s) 94, 983, 1006, 1466, 1479
Tru1I TTAA 7 cut(s) 48, 213, 681, 828, 912, 1089, 1236
Tru9I TTAA 7 cut(s) 48, 213, 681, 828, 912, 1089, 1236
TseFI GTSAC 2 cut(s) 651, 1282
TseI GCWGC 2 cut(s) 277, 1429
Tsp45I GTSAC 2 cut(s) 651, 1282
TspGWI ACGGA 1 cut(s) 584
Van91I CCANNNNNTGG 1 cut(s) 1076
VspI ATTAAT 1 cut(s) 1089
XapI RAATTY 2 cut(s) 548, 877
XhoI CTCGAG 1 cut(s) 1020
XmiI GTMKAC 1 cut(s) 1313
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.