Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0009286

Physical Location & Seq

Genomic Coordinates Forward (+)

27578026 .. 27579546

Gene Structure 1521 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1228100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1521 bp

ATGGTCTTCCATATTCAGAAATGCTTTAAAAGCCTTCGCTTCCGACGCAATACTTCAAAACCTTGCTCCTTTTTCCATACCTCTCCTGAAAATTGCTCATCTGCTCTTTCTTCAATTTTTTCTTCTCCGCTGGGATCGTCACAGTTTGAAGACTCTACAAGACTATCTGACCTTGACATCATCATATCTAAAGTATCTGTTGGAAGCAGTGATGATGAAGTTCTACGGTCTCTTGCACAAGATCAAGCATGTAATAGCATTCAGCTCACGGATAAACTTGTTGATCAACTGTTTCATCGGTTTAAGAATGATTGGAAGTCTGCATTGGGTGTATTTAGATGGGTAGAGTTATGCTCCGACTATAAACCCACCCCAGATGCATATGATTTGATAGTTGACATTTTGGGTAAAATGAAGCAATTGGATAAGATAACGGGTTTGTTAGAAAAAATGCGCCAGGATCATCTGGTGACTCTTAACACCGTAGGTAAGGTTATGAGAAGGTTTGCTGGTGCAGGGAAATGGGAGGACTCTGTGAGGATATTTGATGATTTAGGAAGTTTTGGGTTGGAGAAGAACACAGAATCTATGAACTTATTGCTTGACACCCTTTGCAAGGAGAACAAAGTTGAGAAGGCCCGTGAGATCTTCTTAGAGCTGAAATCACATATCTCGTCCAATGCTCATACCTTTAACATTTTCATTCACGGTTGGTGCAAGATCAAACGGGTTGACGAGGCACATTGGACAATCCAAGAGATGAAGGGACATGGCTATCGCCCTTGTGTCATCAGTTACTCGACCATCATCCTGTTTTATTGCCACCAATACAATTTTGGTAAGGTCTATGAATTGTTTGATGAAATGGAAGCTCAGGGGTGCTCCCCAAATGTCGTCACATATACCTCTGTCATGTGTTTTCTAGCAAAGTCCGAGGAGTTTGAGGAAGCTTTGCAGTTATATGAGAGAATGAAATCTGCTGGATGTGATCCCGATACACTTTTCTACAATTCACTGATCCACACACTAGGAAGAGCTGGCCGAGTCCGAGAGGCTGTCCATGTCTTCGACGTTGAAATGCCTGAGAAGGGGGTTCCTCCTAATACATCAACTTACAATACAATGATTGCCATGTTTTGCCACCACGGGGAAGAGCAAAAGGCCTTTAATGTCCTCCTGCAGATGGAAAATGCAGGGTTATGCAAACCTGATGTTCAGACGTACTATCCATTGCTTAAAATGTGCTTCAAAACTGGAAAAGTAGACGATTGTTTGACTCAATTATTGAATGACATGGTCAATAGACATCATCTCAGTCTCGACCTCTCCAGCTATACTCTTTTGATTCACGGACTATGCAGAGCAAATAGATGCGAGTGGGCTTACCTTCTCTTCAAGGAGATGATTGGTCAAGAGATAAAGCCTAGGTACAAGACATGTCGTTTTCTCTGGGATGAAGTGAAGCTGAAGAATATGTTTGCCGCTGCTGATACCATTGAAGATTTCATGAAGAAACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

507

Amino Acids

58.25

Weight (kDa)

6.5

Isoelectric Point (pI)

31.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	193 - 240	2.4e-09	PPR repeat family
PPR_2	PF13041	227 - 270	3.9e-07	PPR repeat family
PPR_2	PF13041	261 - 309	3.7e-12	PPR repeat family
PPR_long	PF17177	281 - 355	8.8e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	285 - 344	1.6e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	296 - 333	1.4e-09	PPR repeat family
PPR	PF01535	299 - 329	3.6e-06	PPR repeat
PPR_long	PF17177	309 - 426	3.8e-11	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	328 - 357	1.4e-07	PPR repeat
PPR_2	PF13041	331 - 381	4.9e-13	PPR repeat family
TPR_24	PF23276	340 - 467	1.2e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	359 - 415	3.1e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	363 - 396	3.3e-07	PPR repeat
PPR_2	PF13041	367 - 417	3.7e-10	PPR repeat family
PPR_1	PF12854	438 - 468	2.1e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0009286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04130 AT3G04130
fragaria_vesca	FvH4_4g36630 FvH4_5g29890 FvH4_5g29890
malus_domestica	MD17G1227800.v1.1 MD17G1228100.v1.1
prunus_persica	Prupe.3G105000_v2.0.a1 Prupe.3G105000_v2.0.a1
pyrus_communis	pycom17g23070
rosa_chinensis	RchiOBHm_Chr7g0223501
rosa_laevigata	RLG00000001960
rosa_multiflora	Rmu_sc0002224.1_g000003
rosa_roxburghii	Rroxscaffold_3G00235660
rosa_rugosa	Rorug07G0217700 Rorug07G0217700 Rorug07G0217700
rosa_samantha	Rh7AG361100 Rh7BG351600 Rh7CG379600 Rh7DG356400
rosa_wichuraiana	Rw7G030690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1263
AciI	CCGC	2 cut(s)	128, 1482
AclWI	GGATC	4 cut(s)	142, 468, 983, 1012
AcoI	YGGCCR	1 cut(s)	1039
AcuI	CTGAAG	1 cut(s)	1487
AfaI	GTAC	2 cut(s)	1223, 1430
AfiI	CCNNNNNNNGG	4 cut(s)	616, 1088, 1147, 1183
AflIII	ACRYGT	1 cut(s)	1436
AgsI	TTSAA	8 cut(s)	57, 114, 149, 1076, 1249, 1288, 1396, 1499
AjnI	CCWGG	1 cut(s)	456
AjuI	GAANNNNNNNTTGG	2 cut(s)	308, 340
AluBI	AGCT	7 cut(s)	265, 658, 872, 950, 1037, 1332, 1465
AluI	AGCT	7 cut(s)	265, 658, 872, 950, 1037, 1332, 1465
Alw21I	GWGCWC	1 cut(s)	884
Alw26I	GTCTC	2 cut(s)	234, 1322
AlwI	GGATC	4 cut(s)	142, 468, 983, 1012
AoxI	GGCC	3 cut(s)	636, 1039, 1161
ApeKI	GCWGC	1 cut(s)	1484
AspA2I	CCTAGG	1 cut(s)	1424
AspLEI	GCGC	1 cut(s)	456
AspS9I	GGNCC	1 cut(s)	637
AsuHPI	GGTGA	1 cut(s)	481
AvrII	CCTAGG	1 cut(s)	1424
BbsI	GAAGAC	2 cut(s)	156, 1057
Bbv12I	GWGCWC	1 cut(s)	884
BbvI	GCAGC	1 cut(s)	1471
BccI	CCATC	3 cut(s)	333, 812, 1177
BciT130I	CCWGG	1 cut(s)	458
BclI	TGATCA	1 cut(s)	283
BcoDI	GTCTC	2 cut(s)	234, 1322
BfaI	CTAG	3 cut(s)	923, 1028, 1425
BfmI	CTRYAG	2 cut(s)	1178, 1517
BglII	AGATCT	1 cut(s)	645
BisI	GCNGC	2 cut(s)	1482, 1485
BlnI	CCTAGG	1 cut(s)	1424
BlsI	GCNGC	2 cut(s)	1483, 1486
Bme1390I	CCNGG	1 cut(s)	458
BmgT120I	GGNCC	1 cut(s)	637
BmiI	GGNNCC	1 cut(s)	1095
BmrFI	CCNGG	1 cut(s)	458
BmsI	GCATC	2 cut(s)	367, 1361
BpiI	GAAGAC	2 cut(s)	156, 1057
BplI	GAGNNNNNCTC	2 cut(s)	338, 370
BpmI	CTGGAG	1 cut(s)	1312
Bpu10I	CCTNAGC	1 cut(s)	873
BsaI	GGTCTC	1 cut(s)	234
BsaJI	CCNNGG	3 cut(s)	933, 1144, 1424
Bsc4I	CCNNNNNNNGG	4 cut(s)	616, 1088, 1147, 1183
Bse1I	ACTGG	1 cut(s)	1258
Bse3DI	GCAATG	1 cut(s)	1229
BseBI	CCWGG	1 cut(s)	458
BseDI	CCNNGG	3 cut(s)	933, 1144, 1424
BseGI	GGATG	3 cut(s)	807, 989, 1459
BseLI	CCNNNNNNNGG	4 cut(s)	616, 1088, 1147, 1183
BseMI	GCAATG	1 cut(s)	1229
BseMII	CTCAG	3 cut(s)	887, 1074, 1327
BseNI	ACTGG	1 cut(s)	1258
BseRI	GAGGAG	1 cut(s)	950
BseXI	GCAGC	1 cut(s)	1471
BseYI	CCCAGC	1 cut(s)	130
BsgI	GTGCAG	1 cut(s)	534
BshFI	GGCC	3 cut(s)	638, 1041, 1163
BsiHKAI	GWGCWC	1 cut(s)	884
BslFI	GGGAC	1 cut(s)	780
BslI	CCNNNNNNNGG	4 cut(s)	616, 1088, 1147, 1183
BsmAI	GTCTC	2 cut(s)	234, 1322
BsmFI	GGGAC	1 cut(s)	780
BsmI	GAATGC	1 cut(s)	258
BsnI	GGCC	3 cut(s)	638, 1041, 1163
Bso31I	GGTCTC	1 cut(s)	234
Bsp1286I	GDGCHC	1 cut(s)	884
Bsp143I	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
BspACI	CCGC	2 cut(s)	128, 1482
BspANI	GGCC	3 cut(s)	638, 1041, 1163
BspCNI	CTCAG	3 cut(s)	886, 1075, 1326
BspHI	TCATGA	1 cut(s)	1506
BspLI	GGNNCC	1 cut(s)	1095
BspMAI	CTGCAG	1 cut(s)	1182
BspPI	GGATC	4 cut(s)	142, 468, 983, 1012
BspQI	GCTCTTC	2 cut(s)	1027, 1146
BspTNI	GGTCTC	1 cut(s)	234
BsrDI	GCAATG	1 cut(s)	1229
BsrI	ACTGG	1 cut(s)	1258
BssECI	CCNNGG	3 cut(s)	933, 1144, 1424
BssMI	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
BssT1I	CCWWGG	1 cut(s)	1424
Bst2UI	CCWGG	1 cut(s)	458
Bst4CI	ACNGT	6 cut(s)	144, 228, 291, 484, 710, 1518
Bst6I	CTCTTC	3 cut(s)	1027, 1146, 1397
BstC8I	GCNNGC	1 cut(s)	1039
BstDEI	CTNAG	4 cut(s)	652, 873, 1083, 1313
BstDSI	CCRYGG	1 cut(s)	1144
BstENI	CCTNNNNNAGG	1 cut(s)	614
BstF5I	GGATG	3 cut(s)	807, 989, 1459
BstHHI	GCGC	1 cut(s)	456
BstKTI	GATC	8 cut(s)	137, 244, 286, 463, 648, 723, 991, 1020
BstMAI	GTCTC	2 cut(s)	234, 1322
BstMBI	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
BstMWI	GCNNNNNNNGC	2 cut(s)	30, 45
BstNI	CCWGG	1 cut(s)	458
BstNSI	RCATGY	2 cut(s)	252, 1440
BstSCI	CCNGG	1 cut(s)	456
BstSFI	CTRYAG	2 cut(s)	1178, 1517
BstV1I	GCAGC	1 cut(s)	1471
BstV2I	GAAGAC	2 cut(s)	156, 1057
BstX2I	RGATCY	1 cut(s)	645
BstYI	RGATCY	1 cut(s)	645
BsuRI	GGCC	3 cut(s)	638, 1041, 1163
BtgI	CCRYGG	1 cut(s)	1144
BtsCI	GGATG	3 cut(s)	807, 989, 1459
BtsI	GCAGTG	1 cut(s)	214
BtsIMutI	CAGTG	2 cut(s)	214, 1013
Cac8I	GCNNGC	1 cut(s)	1039
CciI	TCATGA	1 cut(s)	1506
CfoI	GCGC	1 cut(s)	456
Cfr13I	GGNCC	1 cut(s)	637
CseI	GACGC	1 cut(s)	54
Csp6I	GTAC	2 cut(s)	1222, 1429
CviAII	CATG	8 cut(s)	249, 770, 913, 1061, 1132, 1294, 1437, 1507
CviQI	GTAC	2 cut(s)	1222, 1429
DdeI	CTNAG	4 cut(s)	652, 873, 1083, 1313
DpnI	GATC	8 cut(s)	136, 243, 285, 462, 647, 722, 990, 1019
DpnII	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
DraI	TTTAAA	1 cut(s)	28
EaeI	YGGCCR	1 cut(s)	1039
Eam1104I	CTCTTC	3 cut(s)	1027, 1146, 1397
EarI	CTCTTC	3 cut(s)	1027, 1146, 1397
Eco130I	CCWWGG	1 cut(s)	1424
Eco147I	AGGCCT	1 cut(s)	1163
Eco31I	GGTCTC	1 cut(s)	234
Eco57I	CTGAAG	1 cut(s)	1487
EcoNI	CCTNNNNNAGG	1 cut(s)	614
EcoRII	CCWGG	1 cut(s)	456
EcoT14I	CCWWGG	1 cut(s)	1424
EcoT22I	ATGCAT	1 cut(s)	382
ErhI	CCWWGG	1 cut(s)	1424
FaeI	CATG	8 cut(s)	252, 773, 916, 1064, 1135, 1297, 1440, 1510
FaqI	GGGAC	1 cut(s)	780
FatI	CATG	8 cut(s)	248, 769, 912, 1060, 1131, 1293, 1436, 1506
FauNDI	CATATG	1 cut(s)	382
FbaI	TGATCA	1 cut(s)	283
FblI	GTMKAC	1 cut(s)	1263
Fnu4HI	GCNGC	2 cut(s)	1482, 1485
FokI	GGATG	3 cut(s)	794, 996, 1466
Fsp4HI	GCNGC	2 cut(s)	1482, 1485
FspBI	CTAG	3 cut(s)	923, 1028, 1425
GlaI	GCGC	1 cut(s)	455
GluI	GCNGC	2 cut(s)	1482, 1485
GsaI	CCCAGC	1 cut(s)	134
GsuI	CTGGAG	1 cut(s)	1312
HaeIII	GGCC	3 cut(s)	638, 1041, 1163
HgaI	GACGC	1 cut(s)	54
HhaI	GCGC	1 cut(s)	456
Hin1II	CATG	8 cut(s)	252, 773, 916, 1064, 1135, 1297, 1440, 1510
Hin6I	GCGC	1 cut(s)	454
HinP1I	GCGC	1 cut(s)	454
HincII	GTYRAC	2 cut(s)	397, 733
HindII	GTYRAC	2 cut(s)	397, 733
HindIII	AAGCTT	1 cut(s)	948
HinfI	GANTC	7 cut(s)	152, 472, 530, 584, 1044, 1276, 1345
HphI	GGTGA	1 cut(s)	481
Hpy166II	GTNNAC	3 cut(s)	397, 733, 1264
Hpy188I	TCNGA	7 cut(s)	18, 44, 169, 358, 934, 1049, 1218
Hpy188III	TCNNGA	5 cut(s)	86, 992, 1319, 1412, 1507
Hpy8I	GTNNAC	3 cut(s)	397, 733, 1264
Hpy99I	CGWCG	2 cut(s)	48, 1073
HpyAV	CCTTC	6 cut(s)	44, 495, 628, 757, 1081, 1397
HpyCH4III	ACNGT	6 cut(s)	144, 228, 291, 484, 710, 1518
HpyCH4IV	ACGT	2 cut(s)	1071, 1220
HpyF10VI	GCNNNNNNNGC	2 cut(s)	30, 45
HpyF3I	CTNAG	4 cut(s)	652, 873, 1083, 1313
HpySE526I	ACGT	2 cut(s)	1071, 1220
Hsp92II	CATG	8 cut(s)	252, 773, 916, 1064, 1135, 1297, 1440, 1510
HspAI	GCGC	1 cut(s)	454
Ksp22I	TGATCA	1 cut(s)	283
Kzo9I	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
LguI	GCTCTTC	2 cut(s)	1027, 1146
LmnI	GCTCC	3 cut(s)	71, 359, 887
Lsp1109I	GCAGC	1 cut(s)	1471
LweI	GCATC	2 cut(s)	367, 1361
MaeI	CTAG	3 cut(s)	923, 1028, 1425
MaeII	ACGT	2 cut(s)	1071, 1220
MaeIII	GTNAC	4 cut(s)	138, 469, 794, 895
MalI	GATC	8 cut(s)	136, 243, 285, 462, 647, 722, 990, 1019
MboI	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
MfeI	CAATTG	1 cut(s)	419
MflI	RGATCY	1 cut(s)	645
MhlI	GDGCHC	1 cut(s)	884
MluCI	AATT	7 cut(s)	91, 114, 419, 832, 851, 1009, 1280
MlyI	GAGTC	5 cut(s)	146, 466, 524, 1053, 1270
MmeI	TCCRAC	4 cut(s)	67, 181, 381, 549
Mph1103I	ATGCAT	1 cut(s)	382
MseI	TTAA	6 cut(s)	27, 303, 477, 693, 1167, 1236
MspA1I	CMGCKG	2 cut(s)	130, 1484
MspR9I	CCNGG	1 cut(s)	458
MunI	CAATTG	1 cut(s)	419
Mva1269I	GAATGC	1 cut(s)	258
MvaI	CCWGG	1 cut(s)	458
MwoI	GCNNNNNNNGC	2 cut(s)	30, 45
NdeI	CATATG	1 cut(s)	382
NdeII	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
NlaIII	CATG	8 cut(s)	252, 773, 916, 1064, 1135, 1297, 1440, 1510
NlaIV	GGNNCC	1 cut(s)	1095
NmeAIII	GCCGAG	1 cut(s)	1067
NmuCI	GTSAC	3 cut(s)	138, 469, 895
NsiI	ATGCAT	1 cut(s)	382
NspI	RCATGY	2 cut(s)	252, 1440
PagI	TCATGA	1 cut(s)	1506
PceI	AGGCCT	1 cut(s)	1163
PciI	ACATGT	1 cut(s)	1436
PciSI	GCTCTTC	2 cut(s)	1027, 1146
PctI	GAATGC	1 cut(s)	258
PfeI	GAWTC	2 cut(s)	584, 1345
PflFI	GACNNNGTC	1 cut(s)	1295
PkrI	GCNGC	2 cut(s)	1483, 1486
PleI	GAGTC	5 cut(s)	146, 466, 524, 1052, 1270
PpsI	GAGTC	5 cut(s)	146, 466, 524, 1052, 1270
PscI	ACATGT	1 cut(s)	1436
Psp6I	CCWGG	1 cut(s)	456
PspFI	CCCAGC	1 cut(s)	130
PspGI	CCWGG	1 cut(s)	456
PspN4I	GGNNCC	1 cut(s)	1095
PspPI	GGNCC	1 cut(s)	637
PstI	CTGCAG	1 cut(s)	1182
PsuI	RGATCY	1 cut(s)	645
PsyI	GACNNNGTC	1 cut(s)	1295
RsaI	GTAC	2 cut(s)	1223, 1430
RsaNI	GTAC	2 cut(s)	1222, 1429
SapI	GCTCTTC	2 cut(s)	1027, 1146
SaqAI	TTAA	6 cut(s)	27, 303, 477, 693, 1167, 1236
SatI	GCNGC	2 cut(s)	1482, 1485
Sau3AI	GATC	8 cut(s)	134, 241, 283, 460, 645, 720, 988, 1017
Sau96I	GGNCC	1 cut(s)	637
SchI	GAGTC	5 cut(s)	146, 466, 524, 1053, 1270
ScrFI	CCNGG	1 cut(s)	458
SduI	GDGCHC	1 cut(s)	884
SfaNI	GCATC	2 cut(s)	367, 1361
SfcI	CTRYAG	2 cut(s)	1178, 1517
Sse9I	AATT	7 cut(s)	91, 114, 419, 832, 851, 1009, 1280
SseBI	AGGCCT	1 cut(s)	1163
SsiI	CCGC	2 cut(s)	128, 1482
SspMI	CTAG	3 cut(s)	923, 1028, 1425
StuI	AGGCCT	1 cut(s)	1163
StyD4I	CCNGG	1 cut(s)	456
StyI	CCWWGG	1 cut(s)	1424
TaaI	ACNGT	6 cut(s)	144, 228, 291, 484, 710, 1518
TaiI	ACGT	2 cut(s)	1074, 1223
TaqI	TCGA	3 cut(s)	800, 1068, 1320
TasI	AATT	7 cut(s)	91, 114, 419, 832, 851, 1009, 1280
TauI	GCSGC	1 cut(s)	1484
TfiI	GAWTC	2 cut(s)	584, 1345
Tru1I	TTAA	6 cut(s)	27, 303, 477, 693, 1167, 1236
Tru9I	TTAA	6 cut(s)	27, 303, 477, 693, 1167, 1236
TscAI	CASTG	2 cut(s)	214, 1020
TseFI	GTSAC	3 cut(s)	138, 469, 895
TseI	GCWGC	1 cut(s)	1484
Tsp45I	GTSAC	3 cut(s)	138, 469, 895
TspGWI	ACGGA	2 cut(s)	284, 1365
TspRI	CASTG	2 cut(s)	214, 1020
Tth111I	GACNNNGTC	1 cut(s)	1295
XagI	CCTNNNNNAGG	1 cut(s)	614
XceI	RCATGY	2 cut(s)	252, 1440
XcmI	CCANNNNNNNNNTGG	1 cut(s)	833
XmaJI	CCTAGG	1 cut(s)	1424
XmiI	GTMKAC	1 cut(s)	1263
XspI	CTAG	3 cut(s)	923, 1028, 1425
Zsp2I	ATGCAT	1 cut(s)	382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.