MD17G1227800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009286
Physical Location & Seq
Forward (+)
27560246 .. 27564039
3794 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1227800.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGAAGCAATTGGATAAGATAACGGGTTTGTTAGAAAAAATGCGCCAGGATCATCTGGTGACTCTTAACACCGTAGGTAAGGTTATGAGAAGGTTTGCTGGTGCAGGGAAATGGGAGGACTCTGTGAGGATATTTGATGATTTAGGAAGTTTTGGGTTGGAGAAGAACACAGAATCTATGAACTTATTGCTTGACACCCTTTGCAAGGAGAACAAAGTTGAGAAGGCCCGTGAGATCTTCTTAGAGCTGAAATCACATATCTCGTCCAATGCTCATACCTTTAACATTTTCATTCACGGTTGGTGCAAGATCAAACGGGTTGACGAGGCACATTGGACAATCCAAGAGATGAAGGGACATGGCTATCGCCCTTGTGTCATCAGTTACTCGACCATCATCCTGTTTTATTGCCACCAATACAATTTTGGTAAGGTCTATGAATTGTTTGATGAAATGGAAGCTCAGGGGTGCTCCCCAAATGTCGTCACATATACCTCTGTCATGTGTTTTCTAGCAAAGTCCGAGGAGTTTGAGGAAGCTTTGCAGTTATATGAGAGAATGAAATCTGCTGGATGTGATCCCGATACACTTTTCTACAATTCACTGATCCACACACTAGGAAGAGCTGGCCGAGTCCGAGAGGCTGTCCATGTCTTCGACGTTGAAATGCCTGAGAAGGGGGTTCCTCCTAATACATCAACTTACAATACAATGATTGCCATGTTTTGCCACCACGGGGAAGAGCAAAAGGCCTTTAATGTCCTCCTGCAGATGGAAAATGCAGGGTTATGCAAACCTGATGTTCAGACGTACTATCCATTGCTTAAAATGTGCTTCAAAACTGGAAAAGTAGACGATTGTTTGACTCAATTATTGAATGACATGGTCAATAGACATCATCTCAGTCTCGACCTCTCCAGCTATACTCTTTTGATTCACGGACTATGCAGAGCAAATAGATGCGAGTGGGCTTACCTTCTCTTCAAGGAGATGATTGGTCAAGAGATAAAGCCTAGGTACAAGACATGTCGTTTTCTCTGGGATGAAGTGAAGCTGAAGAATATGTTTGCCGCTGCTGATACCATTGAAGATTTCATGAAGAAACTGTAG

Protein Analysis

370

Amino Acids

42.77

Weight (kDa)

6.54

Isoelectric Point (pI)

26.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 56 - 103 1.5e-09 PPR repeat family
PPR_2 PF13041 90 - 133 2.6e-07 PPR repeat family
PPR_2 PF13041 124 - 172 2.4e-12 PPR repeat family
PPR_long PF17177 144 - 218 4.6e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 148 - 207 1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 156 - 188 8.1e-06 PPR repeat
PPR_2 PF13041 160 - 196 7.6e-09 PPR repeat family
PPR PF01535 162 - 192 2.4e-06 PPR repeat
PPR_long PF17177 172 - 315 8.6e-12 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 191 - 220 9.3e-08 PPR repeat
PPR_2 PF13041 194 - 244 3.2e-13 PPR repeat family
TPR_24 PF23276 203 - 330 8.5e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 222 - 278 2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 226 - 259 2.2e-07 PPR repeat
PPR_2 PF13041 230 - 280 2.5e-10 PPR repeat family
PPR PF01535 233 - 263 1e-05 PPR repeat
PPR_1 PF12854 301 - 331 1.4e-07 PPR repeat
PPR PF01535 307 - 333 8e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 852
AciI CCGC 1 cut(s) 1071
AclWI GGATC 3 cut(s) 57, 572, 601
AcoI YGGCCR 1 cut(s) 628
AcuI CTGAAG 1 cut(s) 1076
AfaI GTAC 2 cut(s) 812, 1019
AfiI CCNNNNNNNGG 4 cut(s) 205, 677, 736, 772
AflIII ACRYGT 1 cut(s) 1025
AgsI TTSAA 5 cut(s) 665, 838, 877, 985, 1088
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 6 cut(s) 247, 461, 539, 626, 921, 1054
AluI AGCT 6 cut(s) 247, 461, 539, 626, 921, 1054
Alw21I GWGCWC 1 cut(s) 473
Alw26I GTCTC 1 cut(s) 911
AlwI GGATC 3 cut(s) 57, 572, 601
AoxI GGCC 3 cut(s) 225, 628, 750
ApeKI GCWGC 1 cut(s) 1073
AspA2I CCTAGG 1 cut(s) 1013
AspLEI GCGC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 70
AvrII CCTAGG 1 cut(s) 1013
BbsI GAAGAC 1 cut(s) 646
Bbv12I GWGCWC 1 cut(s) 473
BbvI GCAGC 1 cut(s) 1060
BccI CCATC 2 cut(s) 401, 766
BciT130I CCWGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 911
BfaI CTAG 3 cut(s) 512, 617, 1014
BfmI CTRYAG 2 cut(s) 767, 1106
BglII AGATCT 1 cut(s) 234
BisI GCNGC 2 cut(s) 1071, 1074
BlnI CCTAGG 1 cut(s) 1013
BlsI GCNGC 2 cut(s) 1072, 1075
Bme1390I CCNGG 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 684
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 950
BpiI GAAGAC 1 cut(s) 646
BpmI CTGGAG 1 cut(s) 901
Bpu10I CCTNAGC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 522, 733, 1013
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 677, 736, 772
Bse1I ACTGG 1 cut(s) 847
Bse3DI GCAATG 1 cut(s) 818
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 3 cut(s) 522, 733, 1013
BseGI GGATG 3 cut(s) 396, 578, 1048
BseLI CCNNNNNNNGG 4 cut(s) 205, 677, 736, 772
BseMI GCAATG 1 cut(s) 818
BseMII CTCAG 3 cut(s) 476, 663, 916
BseNI ACTGG 1 cut(s) 847
BseRI GAGGAG 1 cut(s) 539
BseXI GCAGC 1 cut(s) 1060
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 3 cut(s) 227, 630, 752
BsiHKAI GWGCWC 1 cut(s) 473
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 4 cut(s) 205, 677, 736, 772
BsmAI GTCTC 1 cut(s) 911
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 3 cut(s) 227, 630, 752
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 5 cut(s) 49, 234, 309, 577, 606
BspACI CCGC 1 cut(s) 1071
BspANI GGCC 3 cut(s) 227, 630, 752
BspCNI CTCAG 3 cut(s) 475, 664, 915
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 1 cut(s) 684
BspMAI CTGCAG 1 cut(s) 771
BspPI GGATC 3 cut(s) 57, 572, 601
BspQI GCTCTTC 2 cut(s) 616, 735
BsrDI GCAATG 1 cut(s) 818
BsrI ACTGG 1 cut(s) 847
BssECI CCNNGG 3 cut(s) 522, 733, 1013
BssMI GATC 5 cut(s) 49, 234, 309, 577, 606
BssT1I CCWWGG 1 cut(s) 1013
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 3 cut(s) 73, 299, 1107
Bst6I CTCTTC 3 cut(s) 616, 735, 986
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 4 cut(s) 241, 462, 672, 902
BstDSI CCRYGG 1 cut(s) 733
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 3 cut(s) 396, 578, 1048
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 5 cut(s) 52, 237, 312, 580, 609
BstMAI GTCTC 1 cut(s) 911
BstMBI GATC 5 cut(s) 49, 234, 309, 577, 606
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 1 cut(s) 1029
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 2 cut(s) 767, 1106
BstV1I GCAGC 1 cut(s) 1060
BstV2I GAAGAC 1 cut(s) 646
BstX2I RGATCY 1 cut(s) 234
BstYI RGATCY 1 cut(s) 234
BsuRI GGCC 3 cut(s) 227, 630, 752
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 3 cut(s) 396, 578, 1048
BtsIMutI CAGTG 1 cut(s) 602
Cac8I GCNNGC 1 cut(s) 628
CciI TCATGA 1 cut(s) 1095
CfoI GCGC 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 811, 1018
CviAII CATG 7 cut(s) 359, 502, 650, 721, 883, 1026, 1096
CviQI GTAC 2 cut(s) 811, 1018
DdeI CTNAG 4 cut(s) 241, 462, 672, 902
DpnI GATC 5 cut(s) 51, 236, 311, 579, 608
DpnII GATC 5 cut(s) 49, 234, 309, 577, 606
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 616, 735, 986
EarI CTCTTC 3 cut(s) 616, 735, 986
Eco130I CCWWGG 1 cut(s) 1013
Eco147I AGGCCT 1 cut(s) 752
Eco57I CTGAAG 1 cut(s) 1076
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 1013
ErhI CCWWGG 1 cut(s) 1013
FaeI CATG 7 cut(s) 362, 505, 653, 724, 886, 1029, 1099
FaqI GGGAC 1 cut(s) 369
FatI CATG 7 cut(s) 358, 501, 649, 720, 882, 1025, 1095
FblI GTMKAC 1 cut(s) 852
Fnu4HI GCNGC 2 cut(s) 1071, 1074
FokI GGATG 3 cut(s) 383, 585, 1055
Fsp4HI GCNGC 2 cut(s) 1071, 1074
FspBI CTAG 3 cut(s) 512, 617, 1014
GlaI GCGC 1 cut(s) 44
GluI GCNGC 2 cut(s) 1071, 1074
GsuI CTGGAG 1 cut(s) 901
HaeIII GGCC 3 cut(s) 227, 630, 752
HhaI GCGC 1 cut(s) 45
Hin1II CATG 7 cut(s) 362, 505, 653, 724, 886, 1029, 1099
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 6 cut(s) 61, 119, 173, 633, 865, 934
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 2 cut(s) 322, 853
Hpy188I TCNGA 3 cut(s) 523, 638, 807
Hpy188III TCNNGA 4 cut(s) 581, 908, 1001, 1096
Hpy8I GTNNAC 2 cut(s) 322, 853
Hpy99I CGWCG 1 cut(s) 662
HpyAV CCTTC 5 cut(s) 84, 217, 346, 670, 986
HpyCH4III ACNGT 3 cut(s) 73, 299, 1107
HpyCH4IV ACGT 2 cut(s) 660, 809
HpyCH4V TGCA 8 cut(s) 104, 204, 306, 544, 769, 782, 792, 948
HpyF3I CTNAG 4 cut(s) 241, 462, 672, 902
HpySE526I ACGT 2 cut(s) 660, 809
Hsp92II CATG 7 cut(s) 362, 505, 653, 724, 886, 1029, 1099
HspAI GCGC 1 cut(s) 43
Kzo9I GATC 5 cut(s) 49, 234, 309, 577, 606
LguI GCTCTTC 2 cut(s) 616, 735
LmnI GCTCC 1 cut(s) 476
Lsp1109I GCAGC 1 cut(s) 1060
LweI GCATC 1 cut(s) 950
MaeI CTAG 3 cut(s) 512, 617, 1014
MaeII ACGT 2 cut(s) 660, 809
MaeIII GTNAC 3 cut(s) 58, 383, 484
MalI GATC 5 cut(s) 51, 236, 311, 579, 608
MboI GATC 5 cut(s) 49, 234, 309, 577, 606
MboII GAAGA 8 cut(s) 175, 229, 633, 646, 752, 973, 1069, 1100
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 5 cut(s) 8, 421, 440, 598, 869
MlyI GAGTC 4 cut(s) 55, 113, 642, 859
MmeI TCCRAC 1 cut(s) 138
MseI TTAA 4 cut(s) 66, 282, 756, 825
MspA1I CMGCKG 1 cut(s) 1073
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 8
MvaI CCWGG 1 cut(s) 47
NdeII GATC 5 cut(s) 49, 234, 309, 577, 606
NlaIII CATG 7 cut(s) 362, 505, 653, 724, 886, 1029, 1099
NlaIV GGNNCC 1 cut(s) 684
NmeAIII GCCGAG 1 cut(s) 656
NmuCI GTSAC 2 cut(s) 58, 484
NspI RCATGY 1 cut(s) 1029
PagI TCATGA 1 cut(s) 1095
PceI AGGCCT 1 cut(s) 752
PciI ACATGT 1 cut(s) 1025
PciSI GCTCTTC 2 cut(s) 616, 735
PfeI GAWTC 2 cut(s) 173, 934
PflFI GACNNNGTC 1 cut(s) 884
PkrI GCNGC 2 cut(s) 1072, 1075
PleI GAGTC 4 cut(s) 55, 113, 641, 859
PpsI GAGTC 4 cut(s) 55, 113, 641, 859
PscI ACATGT 1 cut(s) 1025
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 684
PspPI GGNCC 1 cut(s) 226
PstI CTGCAG 1 cut(s) 771
PsuI RGATCY 1 cut(s) 234
PsyI GACNNNGTC 1 cut(s) 884
RsaI GTAC 2 cut(s) 812, 1019
RsaNI GTAC 2 cut(s) 811, 1018
SapI GCTCTTC 2 cut(s) 616, 735
SaqAI TTAA 4 cut(s) 66, 282, 756, 825
SatI GCNGC 2 cut(s) 1071, 1074
Sau3AI GATC 5 cut(s) 49, 234, 309, 577, 606
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 4 cut(s) 55, 113, 642, 859
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 473
SfaNI GCATC 1 cut(s) 950
SfcI CTRYAG 2 cut(s) 767, 1106
Sse9I AATT 5 cut(s) 8, 421, 440, 598, 869
SseBI AGGCCT 1 cut(s) 752
SsiI CCGC 1 cut(s) 1071
SspMI CTAG 3 cut(s) 512, 617, 1014
StuI AGGCCT 1 cut(s) 752
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 1013
TaaI ACNGT 3 cut(s) 73, 299, 1107
TaiI ACGT 2 cut(s) 663, 812
TaqI TCGA 3 cut(s) 389, 657, 909
TasI AATT 5 cut(s) 8, 421, 440, 598, 869
TauI GCSGC 1 cut(s) 1073
TfiI GAWTC 2 cut(s) 173, 934
Tru1I TTAA 4 cut(s) 66, 282, 756, 825
Tru9I TTAA 4 cut(s) 66, 282, 756, 825
TscAI CASTG 1 cut(s) 609
TseFI GTSAC 2 cut(s) 58, 484
TseI GCWGC 1 cut(s) 1073
Tsp45I GTSAC 2 cut(s) 58, 484
TspDTI ATGAA 9 cut(s) 17, 194, 280, 365, 453, 465, 575, 1059, 1084
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 1 cut(s) 609
Tth111I GACNNNGTC 1 cut(s) 884
XagI CCTNNNNNAGG 1 cut(s) 203
XceI RCATGY 1 cut(s) 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 422
XmaJI CCTAGG 1 cut(s) 1013
XmiI GTMKAC 1 cut(s) 852
XspI CTAG 3 cut(s) 512, 617, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.