AT3G07220

multicellular organism development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0015944
Physical Location & Seq
Forward (+)
2297886 .. 2299572
1687 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07220.1

Sequence Viewer

Length: 963 bp
ATGGCTACGGCTGTTGGTGGCGGAAGCGATGTGGAGGTTGGATTTGCGAAGCTTCAAGGTGAGGATTTCGAGTACTATATGCAGTCTTACTCCATTATACTCGGCCGGAATTCTAAGAAAGCCACCGTCGACGTTGATCTCTCATCCCTCGGCGGTGGGATGAACATCTCGCGCAACCACGCTCGGATCTTCTATGACTTCACTAGACGACGCTTCTCTCTCGAGGTCCTTGGCAAAAATGGCTGCCTCGTTGAAGGTGTTCTTCATCTCCCTGGGAATCCTAACGTCAAGCTCGATTCACAAGACCTTTTGCAGATCGGAGACAAAGAGTTCTACTTTCTCCTACCGGTTCGGAGCATCTTAGGCGGGCCGTTGGGACCTAGGCACCACGTCTCTGGGCAAACAAGTGTTGTTCCATACCATAATTATCAGTCGGGTCCAGGTTCTGGGTCGGGTAAGAAGGGCGTCAGGAGTAGAGAGTTGTATGAGTACGATGATGAAGATGATGATGACGACGACGATGAGGAGGACGATATGAGAGGAAGTGGAAAGAAAACAAGGAGAGATGGACATGAAGTAGTATATGCTTCCGGAGAGAAGAAGAGAGAGGGAAGATCAAAGGTAGATCGTGAAGCTGATGATCAACAATTTTTGCAGCTGGAGGAAAAAGATGTTGTATCGTCTGTTGCCACTGTGCTTTCCGATTTGTGTGGTCCGGGAGAGTGGATGCCTATGGAAAAACTTCATTCGGTGATATTAAAGGAGTATGGAAACGTATGGCATCACAGTCGAGTAAGAAGATACCTATCACAAGAAGACTGGGCTATCCCTGAAGCAAAAGGTAAACCATGGTACGGTTTGCTGATGCTGCTGAGAAAATACCCGGAGCATTTCGTCATCAACACGAGATCAAAGGGAAGAGTTACCCTTGAATTCGTTTCCCTCGTTACCCTACTCTCATGA

Protein Analysis

320

Amino Acids

35.99

Weight (kDa)

5.74

Isoelectric Point (pI)

48.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 33 - 107 3.3e-09 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07220
fragaria_vesca FvH4_2g18540
malus_domestica MD05G1171600.v1.1 MD10G1159800.v1.1
prunus_persica Prupe.8G198900_v2.0.a1
pyrus_communis pycom05g15830 pycom10g13800
rosa_chinensis RchiOBHm_Chr6g0284101
rosa_laevigata RLG00000012769
rosa_multiflora Rmu_sc0003778.1_g000044 Rmu_sc0015649.1_g000003
rosa_roxburghii Rroxscaffold_7G00184930
rosa_rugosa Rorug06G0160700.1
rosa_samantha Rh6BG274200
rosa_wichuraiana Rw6G023540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 446
AccI GTMKAC 1 cut(s) 129
AccII CGCG 1 cut(s) 172
AccIII TCCGGA 1 cut(s) 590
AciI CCGC 3 cut(s) 21, 153, 366
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 2 cut(s) 109, 932
AcuI CTGAAG 1 cut(s) 852
AcyI GRCGYC 1 cut(s) 465
AfaI GTAC 3 cut(s) 74, 491, 854
AfiI CCNNNNNNNGG 2 cut(s) 446, 854
AgeI ACCGGT 1 cut(s) 346
AgsI TTSAA 3 cut(s) 56, 254, 932
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 2 cut(s) 271, 439
AluBI AGCT 4 cut(s) 52, 292, 635, 658
AluI AGCT 4 cut(s) 52, 292, 635, 658
Alw26I GTCTC 2 cut(s) 315, 397
AlwI GGATC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 221
Aor13HI TCCGGA 1 cut(s) 590
AoxI GGCC 2 cut(s) 103, 368
ApeKI GCWGC 3 cut(s) 243, 655, 868
ApoI RAATTY 2 cut(s) 109, 932
AsiGI ACCGGT 1 cut(s) 346
Asp700I GAANNNNTTC 2 cut(s) 258, 741
AspA2I CCTAGG 1 cut(s) 380
AspLEI GCGC 1 cut(s) 174
AspS9I GGNCC 5 cut(s) 226, 368, 377, 437, 713
AsuC2I CCSGG 2 cut(s) 717, 884
AsuHPI GGTGA 2 cut(s) 71, 763
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 4 cut(s) 226, 377, 437, 713
AvrII CCTAGG 1 cut(s) 380
BanI GGYRCC 1 cut(s) 384
BauI CACGAG 1 cut(s) 904
BbsI GAAGAC 1 cut(s) 822
BbvI GCAGC 3 cut(s) 230, 667, 855
BccI CCATC 1 cut(s) 560
BceAI ACGGC 2 cut(s) 24, 355
BcgI CGANNNNNNTGC 2 cut(s) 770, 804
BciT130I CCWGG 2 cut(s) 273, 441
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 2 cut(s) 717, 884
BcoDI GTCTC 2 cut(s) 315, 397
BfaI CTAG 2 cut(s) 204, 381
BisI GCNGC 3 cut(s) 244, 656, 869
BlnI CCTAGG 1 cut(s) 380
BlsI GCNGC 3 cut(s) 245, 657, 870
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 4 cut(s) 273, 441, 717, 884
Bme18I GGWCC 4 cut(s) 226, 377, 437, 713
BmeT110I CYCGRG 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 5 cut(s) 226, 368, 377, 437, 713
BmiI GGNNCC 3 cut(s) 378, 386, 438
BmrFI CCNGG 4 cut(s) 273, 441, 717, 884
BmrI ACTGGG 1 cut(s) 829
BmsI GCATC 4 cut(s) 366, 717, 790, 855
BmuI ACTGGG 1 cut(s) 829
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 680
BpuMI CCSGG 2 cut(s) 717, 884
BsaBI GATNNNNATC 2 cut(s) 164, 805
BsaHI GRCGYC 1 cut(s) 465
BsaJI CCNNGG 6 cut(s) 148, 229, 271, 272, 380, 848
BsaWI WCCGGW 2 cut(s) 346, 590
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 854
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 1 cut(s) 824
Bse8I GATNNNNATC 2 cut(s) 164, 805
BseAI TCCGGA 1 cut(s) 590
BseBI CCWGG 2 cut(s) 273, 441
BseDI CCNNGG 6 cut(s) 148, 229, 271, 272, 380, 848
BseGI GGATG 3 cut(s) 143, 165, 732
BseJI GATNNNNATC 2 cut(s) 164, 805
BseLI CCNNNNNNNGG 2 cut(s) 446, 854
BseMII CTCAG 1 cut(s) 863
BseNI ACTGG 1 cut(s) 824
BseRI GAGGAG 1 cut(s) 539
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 3 cut(s) 230, 667, 855
Bsh1236I CGCG 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 105, 370
BshNI GGYRCC 1 cut(s) 384
BshTI ACCGGT 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 221
BsiSI CCGG 5 cut(s) 106, 347, 591, 716, 884
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 2 cut(s) 446, 854
BsmAI GTCTC 2 cut(s) 315, 397
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 2 cut(s) 105, 370
BsoBI CYCGRG 1 cut(s) 221
Bsp13I TCCGGA 1 cut(s) 590
Bsp143I GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
Bsp19I CCATGG 1 cut(s) 848
BspACI CCGC 3 cut(s) 21, 153, 366
BspANI GGCC 2 cut(s) 105, 370
BspCNI CTCAG 1 cut(s) 864
BspEI TCCGGA 1 cut(s) 590
BspFNI CGCG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 959
BspLI GGNNCC 3 cut(s) 378, 386, 438
BspPI GGATC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 1 cut(s) 824
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 6 cut(s) 148, 229, 271, 272, 380, 848
BssMI GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
BssNI GRCGYC 1 cut(s) 465
BssSI CACGAG 1 cut(s) 904
BssT1I CCWWGG 3 cut(s) 229, 380, 848
Bst2BI CACGAG 1 cut(s) 904
Bst2UI CCWGG 2 cut(s) 273, 441
Bst4CI ACNGT 4 cut(s) 127, 694, 788, 857
Bst6I CTCTTC 2 cut(s) 596, 913
BstACI GRCGYC 1 cut(s) 465
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 3 cut(s) 114, 361, 872
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 3 cut(s) 143, 165, 732
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 7 cut(s) 139, 189, 318, 617, 628, 643, 911
BstMAI GTCTC 2 cut(s) 315, 397
BstMBI GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 240, 363, 868
BstNI CCWGG 2 cut(s) 273, 441
BstSCI CCNGG 4 cut(s) 271, 439, 715, 882
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 3 cut(s) 230, 667, 855
BstV2I GAAGAC 1 cut(s) 822
BstX2I RGATCY 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 1 cut(s) 186
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 2 cut(s) 105, 370
BtgI CCRYGG 1 cut(s) 848
BtgZI GCGATG 1 cut(s) 42
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 3 cut(s) 143, 165, 732
BtsIMutI CAGTG 1 cut(s) 690
Cac8I GCNNGC 1 cut(s) 368
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 1 cut(s) 959
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 5 cut(s) 226, 368, 377, 437, 713
CseI GACGC 2 cut(s) 219, 454
Csp6I GTAC 3 cut(s) 73, 490, 853
CspAI ACCGGT 1 cut(s) 346
CviAII CATG 3 cut(s) 572, 849, 960
CviQI GTAC 3 cut(s) 73, 490, 853
DdeI CTNAG 3 cut(s) 114, 361, 872
DpnI GATC 7 cut(s) 138, 188, 317, 616, 627, 642, 910
DpnII GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 596, 913
EarI CTCTTC 2 cut(s) 596, 913
EciI GGCGGA 1 cut(s) 36
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 3 cut(s) 229, 380, 848
Eco47I GGWCC 4 cut(s) 226, 377, 437, 713
Eco52I CGGCCG 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 852
Eco88I CYCGRG 1 cut(s) 221
EcoO109I RGGNCCY 2 cut(s) 226, 377
EcoRI GAATTC 2 cut(s) 109, 932
EcoRII CCWGG 2 cut(s) 271, 439
EcoT14I CCWWGG 3 cut(s) 229, 380, 848
ErhI CCWWGG 3 cut(s) 229, 380, 848
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 3 cut(s) 575, 852, 963
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 1 cut(s) 390
FatI CATG 3 cut(s) 571, 848, 959
FauI CCCGC 1 cut(s) 359
FbaI TGATCA 1 cut(s) 640
FblI GTMKAC 1 cut(s) 129
Fnu4HI GCNGC 3 cut(s) 244, 656, 869
FokI GGATG 3 cut(s) 130, 172, 739
Fsp4HI GCNGC 3 cut(s) 244, 656, 869
FspBI CTAG 2 cut(s) 204, 381
GlaI GCGC 1 cut(s) 173
GluI GCNGC 3 cut(s) 244, 656, 869
GsuI CTGGAG 1 cut(s) 680
HaeIII GGCC 2 cut(s) 105, 370
HapII CCGG 5 cut(s) 106, 347, 591, 716, 884
HgaI GACGC 2 cut(s) 219, 454
HhaI GCGC 1 cut(s) 174
Hin1I GRCGYC 1 cut(s) 465
Hin1II CATG 3 cut(s) 575, 852, 963
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 2 cut(s) 277, 296
HpaII CCGG 5 cut(s) 106, 347, 591, 716, 884
HphI GGTGA 2 cut(s) 71, 763
Hpy166II GTNNAC 2 cut(s) 130, 845
Hpy188I TCNGA 4 cut(s) 186, 320, 354, 703
Hpy188III TCNNGA 5 cut(s) 221, 469, 591, 629, 960
Hpy8I GTNNAC 2 cut(s) 130, 845
Hpy99I CGWCG 5 cut(s) 131, 134, 213, 518, 521
HpyAV CCTTC 2 cut(s) 248, 454
HpyCH4III ACNGT 4 cut(s) 127, 694, 788, 857
HpyCH4IV ACGT 4 cut(s) 132, 285, 390, 774
HpyCH4V TGCA 3 cut(s) 82, 313, 655
HpyF10VI GCNNNNNNNGC 3 cut(s) 240, 363, 868
HpyF3I CTNAG 3 cut(s) 114, 361, 872
HpySE526I ACGT 4 cut(s) 132, 285, 390, 774
Hsp92I GRCGYC 1 cut(s) 465
Hsp92II CATG 3 cut(s) 575, 852, 963
HspAI GCGC 1 cut(s) 172
Kpn2I TCCGGA 1 cut(s) 590
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
LmnI GCTCC 2 cut(s) 354, 886
Lsp1109I GCAGC 3 cut(s) 230, 667, 855
LweI GCATC 4 cut(s) 366, 717, 790, 855
MaeI CTAG 2 cut(s) 204, 381
MaeII ACGT 4 cut(s) 132, 285, 390, 774
MaeIII GTNAC 2 cut(s) 922, 946
MalI GATC 7 cut(s) 138, 188, 317, 616, 627, 642, 910
MboI GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
MboII GAAGA 9 cut(s) 181, 254, 512, 610, 613, 624, 810, 827, 930
MflI RGATCY 1 cut(s) 186
MluCI AATT 4 cut(s) 109, 424, 647, 932
MmeI TCCRAC 1 cut(s) 19
MroI TCCGGA 1 cut(s) 590
MroXI GAANNNNTTC 2 cut(s) 258, 741
MseI TTAA 1 cut(s) 758
MspA1I CMGCKG 1 cut(s) 658
MspI CCGG 5 cut(s) 106, 347, 591, 716, 884
MspR9I CCNGG 4 cut(s) 273, 441, 717, 884
MvaI CCWGG 2 cut(s) 273, 441
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 3 cut(s) 240, 363, 868
NciI CCSGG 2 cut(s) 717, 884
NcoI CCATGG 1 cut(s) 848
NdeII GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
NlaIII CATG 3 cut(s) 575, 852, 963
NlaIV GGNNCC 3 cut(s) 378, 386, 438
NmeAIII GCCGAG 2 cut(s) 81, 129
PaeR7I CTCGAG 1 cut(s) 221
PagI TCATGA 1 cut(s) 959
PasI CCCWGGG 1 cut(s) 272
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 942
PdmI GAANNNNTTC 2 cut(s) 258, 741
PfeI GAWTC 2 cut(s) 277, 296
PflMI CCANNNNNTGG 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 715
PinAI ACCGGT 1 cut(s) 346
PkrI GCNGC 3 cut(s) 245, 657, 870
PpuMI RGGWCCY 2 cut(s) 226, 377
Psp5II RGGWCCY 2 cut(s) 226, 377
Psp6I CCWGG 2 cut(s) 271, 439
PspGI CCWGG 2 cut(s) 271, 439
PspN4I GGNNCC 3 cut(s) 378, 386, 438
PspPI GGNCC 5 cut(s) 226, 368, 377, 437, 713
PspPPI RGGWCCY 2 cut(s) 226, 377
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 658
RsaI GTAC 3 cut(s) 74, 491, 854
RsaNI GTAC 3 cut(s) 73, 490, 853
SalI GTCGAC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 758
SatI GCNGC 3 cut(s) 244, 656, 869
Sau3AI GATC 7 cut(s) 136, 186, 315, 614, 625, 640, 908
Sau96I GGNCC 5 cut(s) 226, 368, 377, 437, 713
ScaI AGTACT 1 cut(s) 74
ScrFI CCNGG 4 cut(s) 273, 441, 717, 884
SfaNI GCATC 4 cut(s) 366, 717, 790, 855
Sfr274I CTCGAG 1 cut(s) 221
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 4 cut(s) 226, 377, 437, 713
SlaI CTCGAG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
Sse9I AATT 4 cut(s) 109, 424, 647, 932
SsiI CCGC 3 cut(s) 21, 153, 366
SspMI CTAG 2 cut(s) 204, 381
StyD4I CCNGG 4 cut(s) 271, 439, 715, 882
StyI CCWWGG 3 cut(s) 229, 380, 848
TaaI ACNGT 4 cut(s) 127, 694, 788, 857
TaiI ACGT 4 cut(s) 135, 288, 393, 777
TaqI TCGA 5 cut(s) 69, 129, 222, 294, 790
TasI AATT 4 cut(s) 109, 424, 647, 932
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 2 cut(s) 277, 296
Tru1I TTAA 1 cut(s) 758
Tru9I TTAA 1 cut(s) 758
TscAI CASTG 1 cut(s) 697
TseI GCWGC 3 cut(s) 243, 655, 868
TspDTI ATGAA 5 cut(s) 176, 254, 513, 588, 734
TspRI CASTG 1 cut(s) 697
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 4 cut(s) 226, 377, 437, 713
XapI RAATTY 2 cut(s) 109, 932
XhoI CTCGAG 1 cut(s) 221
XmaJI CCTAGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 129
XmnI GAANNNNTTC 2 cut(s) 258, 741
XspI CTAG 2 cut(s) 204, 381
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.