MD05G1171600.v1.1

Forkhead associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015944
Physical Location & Seq
Reverse (-)
30060124 .. 30062428
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1171600.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCCGCGAGCAGCAGCGATGTGGAGGCGGGGTTCGCGAAGCTCCAAGGCGAAGACTTCGAATACTACATGCAGACCTACTCCATAATCCTGGGCCGGAACTCCAAGAAATCGACGGTGGACGTCGACCTCTCCAGCCTTGGCGGCGGTATGAACATTTCGCGCCACCACGCGCGAATCTTTTACGACTTCACGCGTCGTCGATTCGCCCTCGAGGTCCTCGGAAAGAACGGCTGCCTCGTGGAGGGCGTACTCCACCTGCCCGGGAACCCGCCGGTCAAGCTCGATTCGCAGGACCTTCTTCAGATCGGAGATAAGGAGTTTTACTTTCTGTTGCCGGTGAGGAGCATTCTCGGTGGGCCTGTGGGGCCTAGGCATTACCCCGGCTCGGTGCCGGTGGTGCCGAGCCCCGTCGGCCCGGCCCATTACGGGTACCACTCGGGTTCGGGCGCGGGGTCGATTGGGAAGAAGGGGAGGGGGAGGGAGTTGTATGAGGACGAGTATGAGGACGAGGAGGATGTTGGTGGGGGGAGTGGGAGTGGGAAGAAGATGAGGAGAGAAGGGTACGATGGTTTCGGATATGGTGGCGGGTCGGGTTCTGGTTCAGGCAAGGCCGGAGCTTTGGAGAAGAAGGGAGATGGAAGGTCAAGGGTTGATCGAGACACTGACAATCAGCAACTTCTGCATTTGGAGGAAAAGGATGTGGTGTCCTCTGTTGCTACTGTGCTCTCTGATCTTTGTGGTCCGGGAGAATGGATGCCTATGGAGAAACTTCATACCGAGTTGGTGGAGCAGTACAGCAGCGTCTGGCATCACACTCGAGTGAGGAGGTATCTCACTTCTGAGGACTGGCCTGGTCCAGAGTCAAAAGGGAAACCGTGGTACGGCTTGCTTATGTTGCTAAGAAAATACCCGGAACACTTTGTCATCAACACAAGGTCCAAGGGCCGGGTAACACTGGAGTTTGTTTCTCTTGTCTCTCTGCTTTCGTAA

Protein Analysis

331

Amino Acids

36.22

Weight (kDa)

6.43

Isoelectric Point (pI)

51.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 30 - 104 2.7e-10 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07220
fragaria_vesca FvH4_2g18540
malus_domestica MD05G1171600.v1.1 MD10G1159800.v1.1
prunus_persica Prupe.8G198900_v2.0.a1
pyrus_communis pycom05g15830 pycom10g13800
rosa_chinensis RchiOBHm_Chr6g0284101
rosa_laevigata RLG00000012769
rosa_multiflora Rmu_sc0003778.1_g000044 Rmu_sc0015649.1_g000003
rosa_roxburghii Rroxscaffold_7G00184930
rosa_rugosa Rorug06G0160700.1
rosa_samantha Rh6BG274200
rosa_wichuraiana Rw6G023540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 267
AatII GACGTC 1 cut(s) 126
AbsI CCTCGAGG 1 cut(s) 212
Acc36I ACCTGC 1 cut(s) 267
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 3 cut(s) 391, 400, 432
AccI GTMKAC 1 cut(s) 126
AccII CGCG 7 cut(s) 8, 38, 163, 173, 175, 196, 452
AciI CCGC 7 cut(s) 6, 29, 144, 147, 272, 452, 588
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 5 cut(s) 252, 434, 566, 797, 884
AfiI CCNNNNNNNGG 5 cut(s) 244, 388, 429, 884, 948
AflIII ACRYGT 1 cut(s) 194
AjnI CCWGG 2 cut(s) 90, 853
AleI CACNNNNGTG 1 cut(s) 821
AloI GAACNNNNNNTCC 2 cut(s) 17, 49
AluBI AGCT 3 cut(s) 43, 283, 620
AluI AGCT 3 cut(s) 43, 283, 620
Alw21I GWGCWC 1 cut(s) 729
Alw26I GTCTC 2 cut(s) 654, 982
AlwNI CAGNNNCTG 1 cut(s) 807
Ama87I CYCGRG 4 cut(s) 212, 263, 439, 819
AoxI GGCC 9 cut(s) 3, 94, 359, 368, 415, 420, 612, 851, 946
ApeKI GCWGC 4 cut(s) 12, 15, 234, 801
Asp718I GGTACC 1 cut(s) 432
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 3 cut(s) 165, 175, 452
AsuC2I CCSGG 7 cut(s) 264, 265, 384, 419, 747, 914, 950
AsuHPI GGTGA 1 cut(s) 352
AsuII TTCGAA 1 cut(s) 60
AvaI CYCGRG 4 cut(s) 212, 263, 439, 819
AvaII GGWCC 5 cut(s) 217, 295, 743, 857, 939
AvrII CCTAGG 1 cut(s) 371
BaeI ACNNNNGTAYC 2 cut(s) 556, 589
BanI GGYRCC 3 cut(s) 391, 400, 432
BanII GRGCYC 1 cut(s) 410
BauI CACGAG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 729
BbvI GCAGC 4 cut(s) 24, 27, 221, 813
BccI CCATC 2 cut(s) 563, 632
BceAI ACGGC 2 cut(s) 247, 901
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 2 cut(s) 92, 855
BcnI CCSGG 7 cut(s) 264, 265, 384, 419, 747, 914, 950
BcoDI GTCTC 2 cut(s) 654, 982
BfaI CTAG 1 cut(s) 372
BfuAI ACCTGC 1 cut(s) 267
BglI GCCNNNNNGGC 3 cut(s) 144, 367, 414
BisI GCNGC 6 cut(s) 6, 13, 16, 145, 235, 802
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 6 cut(s) 7, 14, 17, 146, 236, 803
Bme1390I CCNGG 9 cut(s) 92, 264, 265, 384, 419, 747, 855, 914, 950
Bme18I GGWCC 5 cut(s) 217, 295, 743, 857, 939
BmeT110I CYCGRG 4 cut(s) 212, 263, 439, 819
BmiI GGNNCC 5 cut(s) 269, 369, 393, 402, 434
BmrFI CCNGG 9 cut(s) 92, 264, 265, 384, 419, 747, 855, 914, 950
BmsI GCATC 2 cut(s) 747, 820
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 2 cut(s) 118, 980
Bpu14I TTCGAA 1 cut(s) 60
BpuMI CCSGG 7 cut(s) 264, 265, 384, 419, 747, 914, 950
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 9 cut(s) 46, 91, 139, 220, 263, 371, 382, 878, 942
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 523, 553
Bsc4I CCNNNNNNNGG 5 cut(s) 244, 388, 429, 884, 948
Bse118I RCCGGY 3 cut(s) 274, 337, 394
Bse1I ACTGG 2 cut(s) 854, 963
BseBI CCWGG 2 cut(s) 92, 855
BseDI CCNNGG 9 cut(s) 46, 91, 139, 220, 263, 371, 382, 878, 942
BseGI GGATG 3 cut(s) 523, 706, 762
BseLI CCNNNNNNNGG 5 cut(s) 244, 388, 429, 884, 948
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 2 cut(s) 854, 963
BseRI GAGGAG 4 cut(s) 358, 527, 568, 841
BseXI GCAGC 4 cut(s) 24, 27, 221, 813
Bsh1236I CGCG 7 cut(s) 8, 38, 163, 173, 175, 196, 452
BshFI GGCC 9 cut(s) 5, 96, 361, 370, 417, 422, 614, 853, 948
BshNI GGYRCC 3 cut(s) 391, 400, 432
BsiHKAI GWGCWC 1 cut(s) 729
BsiHKCI CYCGRG 4 cut(s) 212, 263, 439, 819
BslI CCNNNNNNNGG 5 cut(s) 244, 388, 429, 884, 948
BsmAI GTCTC 2 cut(s) 654, 982
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 9 cut(s) 5, 96, 361, 370, 417, 422, 614, 853, 948
BsoBI CYCGRG 4 cut(s) 212, 263, 439, 819
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 2 cut(s) 410, 729
Bsp143I GATC 3 cut(s) 306, 655, 733
Bsp68I TCGCGA 1 cut(s) 38
BspACI CCGC 7 cut(s) 6, 29, 144, 147, 272, 452, 588
BspANI GGCC 9 cut(s) 5, 96, 361, 370, 417, 422, 614, 853, 948
BspCNI CTCAG 1 cut(s) 835
BspFNI CGCG 7 cut(s) 8, 38, 163, 173, 175, 196, 452
BspLI GGNNCC 5 cut(s) 269, 369, 393, 402, 434
BspMI ACCTGC 1 cut(s) 267
BspT104I TTCGAA 1 cut(s) 60
BspT107I GGYRCC 3 cut(s) 391, 400, 432
BsrFI RCCGGY 3 cut(s) 274, 337, 394
BsrI ACTGG 2 cut(s) 854, 963
BssAI RCCGGY 3 cut(s) 274, 337, 394
BssECI CCNNGG 9 cut(s) 46, 91, 139, 220, 263, 371, 382, 878, 942
BssMI GATC 3 cut(s) 306, 655, 733
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 239
BssT1I CCWWGG 4 cut(s) 46, 139, 371, 942
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 92, 855
Bst4CI ACNGT 3 cut(s) 118, 724, 879
BstACI GRCGYC 1 cut(s) 123
BstAPI GCANNNNNTGC 1 cut(s) 682
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 10, 890
BstDEI CTNAG 2 cut(s) 843, 902
BstDSI CCRYGG 1 cut(s) 878
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 3 cut(s) 523, 706, 762
BstFNI CGCG 7 cut(s) 8, 38, 163, 173, 175, 196, 452
BstHHI GCGC 3 cut(s) 165, 175, 452
BstKTI GATC 3 cut(s) 309, 658, 736
BstMAI GTCTC 2 cut(s) 654, 982
BstMBI GATC 3 cut(s) 306, 655, 733
BstMWI GCNNNNNNNGC 9 cut(s) 35, 144, 280, 289, 367, 400, 414, 682, 898
BstNI CCWGG 2 cut(s) 92, 855
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 9 cut(s) 90, 262, 263, 382, 417, 745, 853, 912, 948
BstUI CGCG 7 cut(s) 8, 38, 163, 173, 175, 196, 452
BstV1I GCAGC 4 cut(s) 24, 27, 221, 813
BstV2I GAAGAC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 91
BsuRI GGCC 9 cut(s) 5, 96, 361, 370, 417, 422, 614, 853, 948
BtgI CCRYGG 1 cut(s) 878
BtgZI GCGATG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 523, 706, 762
BtsIMutI CAGTG 2 cut(s) 663, 956
BtuMI TCGCGA 1 cut(s) 38
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 2 cut(s) 10, 890
CaiI CAGNNNCTG 1 cut(s) 807
CfoI GCGC 3 cut(s) 165, 175, 452
Cfr10I RCCGGY 3 cut(s) 274, 337, 394
Cfr9I CCCGGG 1 cut(s) 263
CseI GACGC 2 cut(s) 185, 793
Csp6I GTAC 5 cut(s) 251, 433, 565, 796, 883
CviAII CATG 1 cut(s) 70
CviQI GTAC 5 cut(s) 251, 433, 565, 796, 883
DdeI CTNAG 2 cut(s) 843, 902
DpnI GATC 3 cut(s) 308, 657, 735
DpnII GATC 3 cut(s) 306, 655, 733
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 4 cut(s) 46, 139, 371, 942
Eco24I GRGCYC 1 cut(s) 410
Eco47I GGWCC 5 cut(s) 217, 295, 743, 857, 939
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 4 cut(s) 212, 263, 439, 819
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoO109I RGGNCCY 3 cut(s) 217, 295, 368
EcoRII CCWGG 2 cut(s) 90, 853
EcoT14I CCWWGG 4 cut(s) 46, 139, 371, 942
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 4 cut(s) 46, 139, 371, 942
FaeI CATG 1 cut(s) 73
FaiI YATR 9 cut(s) 71, 86, 152, 492, 504, 582, 764, 777, 896
FatI CATG 1 cut(s) 69
FauI CCCGC 4 cut(s) 22, 279, 445, 581
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 6 cut(s) 6, 13, 16, 145, 235, 802
FokI GGATG 3 cut(s) 530, 713, 769
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 6 cut(s) 6, 13, 16, 145, 235, 802
FspBI CTAG 1 cut(s) 372
GlaI GCGC 3 cut(s) 164, 174, 451
GluI GCNGC 6 cut(s) 6, 13, 16, 145, 235, 802
GsuI CTGGAG 2 cut(s) 118, 980
HaeIII GGCC 9 cut(s) 5, 96, 361, 370, 417, 422, 614, 853, 948
HgaI GACGC 2 cut(s) 185, 793
HhaI GCGC 3 cut(s) 165, 175, 452
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 1 cut(s) 73
Hin6I GCGC 3 cut(s) 163, 173, 450
HinP1I GCGC 3 cut(s) 163, 173, 450
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 4 cut(s) 177, 204, 287, 863
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 2 cut(s) 121, 127
Hpy188I TCNGA 6 cut(s) 224, 306, 311, 578, 733, 844
Hpy188III TCNNGA 3 cut(s) 37, 659, 860
Hpy8I GTNNAC 2 cut(s) 121, 127
Hpy99I CGWCG 5 cut(s) 118, 128, 201, 204, 416
HpyAV CCTTC 5 cut(s) 308, 463, 554, 625, 636
HpyCH4III ACNGT 3 cut(s) 118, 724, 879
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 73, 685
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 144, 280, 289, 367, 400, 414, 682, 898
HpyF3I CTNAG 2 cut(s) 843, 902
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 1 cut(s) 73
HspAI GCGC 3 cut(s) 163, 173, 450
KpnI GGTACC 1 cut(s) 436
Kzo9I GATC 3 cut(s) 306, 655, 733
LmnI GCTCC 4 cut(s) 48, 345, 617, 790
Lsp1109I GCAGC 4 cut(s) 24, 27, 221, 813
LweI GCATC 2 cut(s) 747, 820
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 952
MalI GATC 3 cut(s) 308, 657, 735
MboI GATC 3 cut(s) 306, 655, 733
MboII GAAGA 6 cut(s) 65, 293, 478, 556, 559, 640
MhlI GDGCHC 2 cut(s) 410, 729
MluI ACGCGT 1 cut(s) 194
MlyI GAGTC 1 cut(s) 872
MslI CAYNNNNRTG 1 cut(s) 821
MspR9I CCNGG 9 cut(s) 92, 264, 265, 384, 419, 747, 855, 914, 950
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 2 cut(s) 92, 855
MvnI CGCG 7 cut(s) 8, 38, 163, 173, 175, 196, 452
MwoI GCNNNNNNNGC 9 cut(s) 35, 144, 280, 289, 367, 400, 414, 682, 898
NciI CCSGG 7 cut(s) 264, 265, 384, 419, 747, 914, 950
NdeII GATC 3 cut(s) 306, 655, 733
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 5 cut(s) 269, 369, 393, 402, 434
NmeAIII GCCGAG 1 cut(s) 429
NruI TCGCGA 1 cut(s) 38
NspI RCATGY 1 cut(s) 73
NspV TTCGAA 1 cut(s) 60
OliI CACNNNNGTG 1 cut(s) 821
PaeR7I CTCGAG 2 cut(s) 212, 819
PaqCI CACCTGC 1 cut(s) 267
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 246
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 177, 204, 287
PfoI TCCNGGA 1 cut(s) 745
PkrI GCNGC 6 cut(s) 7, 14, 17, 146, 236, 803
PleI GAGTC 1 cut(s) 871
PpsI GAGTC 1 cut(s) 871
PpuMI RGGWCCY 2 cut(s) 217, 295
Psp5II RGGWCCY 2 cut(s) 217, 295
Psp6I CCWGG 2 cut(s) 90, 853
PspGI CCWGG 2 cut(s) 90, 853
PspN4I GGNNCC 5 cut(s) 269, 369, 393, 402, 434
PspPPI RGGWCCY 2 cut(s) 217, 295
PspXI VCTCGAGB 2 cut(s) 212, 819
PstNI CAGNNNCTG 1 cut(s) 807
RruI TCGCGA 1 cut(s) 38
RsaI GTAC 5 cut(s) 252, 434, 566, 797, 884
RsaNI GTAC 5 cut(s) 251, 433, 565, 796, 883
RseI CAYNNNNRTG 1 cut(s) 821
SalI GTCGAC 1 cut(s) 125
SatI GCNGC 6 cut(s) 6, 13, 16, 145, 235, 802
Sau3AI GATC 3 cut(s) 306, 655, 733
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 9 cut(s) 92, 264, 265, 384, 419, 747, 855, 914, 950
SduI GDGCHC 2 cut(s) 410, 729
SfaNI GCATC 2 cut(s) 747, 820
SfiI GGCCNNNNNGGCC 1 cut(s) 367
Sfr274I CTCGAG 2 cut(s) 212, 819
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 5 cut(s) 217, 295, 743, 857, 939
SlaI CTCGAG 2 cut(s) 212, 819
SmaI CCCGGG 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 821
SmlI CTYRAG 2 cut(s) 212, 819
SmoI CTYRAG 2 cut(s) 212, 819
SsiI CCGC 7 cut(s) 6, 29, 144, 147, 272, 452, 588
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 9 cut(s) 90, 262, 263, 382, 417, 745, 853, 912, 948
StyI CCWWGG 4 cut(s) 46, 139, 371, 942
TaaI ACNGT 3 cut(s) 118, 724, 879
TaiI ACGT 1 cut(s) 126
TaqI TCGA 9 cut(s) 60, 113, 126, 202, 213, 285, 458, 658, 820
TatI WGTACW 1 cut(s) 795
TauI GCSGC 2 cut(s) 8, 147
TfiI GAWTC 3 cut(s) 177, 204, 287
TscAI CASTG 2 cut(s) 670, 963
TseI GCWGC 4 cut(s) 12, 15, 234, 801
TspDTI ATGAA 2 cut(s) 167, 764
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 2 cut(s) 670, 963
VpaK11BI GGWCC 5 cut(s) 217, 295, 743, 857, 939
XagI CCTNNNNNAGG 1 cut(s) 242
XceI RCATGY 1 cut(s) 73
XhoI CTCGAG 2 cut(s) 212, 819
XmaI CCCGGG 1 cut(s) 263
XmaJI CCTAGG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.