MD10G1159800.v1.1

Forkhead associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0015944
Physical Location & Seq
Reverse (-)
25084004 .. 25086873
2870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1159800.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCGGCGGGCAGCACTGATGTGGAGGCGGGGTTCGCGAAGCTTCAAGGCGAAGACTTCGAGTACTACATGCAGACCTACTCCATAATCCTGGGCCGGAACTCCAAGAAATCGACGGTGGACGTTGACCTCTCCAGCCTCGGCGGCGGCATGAACATTTCGCGCCACCATGCGCGAATTTTCTACGACTTCACGCGGCGTCGATTCGCGCTCGAGGTCCTCGGCAAGAACGGCTGCCTCGTGGAGGGTGTGCTCCACCTACCCGGGAACCCCCCGGTCAAGCTGGACTCGCAGGATCTTCTTCAGATCGGAGATAAGGAGTTTTACTTTTTGTTGCCGGTGAGGAGCATTCTCGGTGGTCCTGTGGGTCCCAGGCATTACCCCGCCTCGGTGCCAGTGGTGCCGAGCCCCGTGGGCCCGGCTCATTACGGGTACCATTCGGGTTCGGGTGCTGGCCCGATTGGGAAGAAGGGGAGGGGGAGGGAGTTGTATGAGGAGGAGTATGATGACGAGGAGGATGTTGGTGGGGGCAGTGGGAGTGGGAAGAAGATGAGGCGAGAAGGGTATGAGGGTTTTGGGTATGGTGGCGGGTCGGGTTCTGGTTCAGGCAAGGCTGGAGCTTTGGAGAAGAAAGGAGATGGAAGGTCCAGGGTTGATCGAGACACTGACAATCAGCAACTTCTGCATTTGGAGGAAAAGGATGTTGTGTCATCTGTTGCTACTGTGCTCTCCGATCTTTGCGGTCCAGGAGAATGGATGCCTATGGAGAAACTTCATAATGAGTTGGTGGAGCAGTATAGCAGCATCTGGCATCACACTCGAGTGAGAAGGTATCTCACTTCTGAGGAATGGCCTGGTCCAGAGTCAAAAGGGAAACCATGGTACGGCTTGCTTATGCTGCTAAGAAAATACCCCGAACACTTTGTCATCAACACAAGGTCCAAGGGCCGGGTAACACTGGAGTTTGTTTCTCTCGTCTCTCTGCTTTCATAA

Protein Analysis

331

Amino Acids

36.28

Weight (kDa)

6.43

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FHA PF00498 30 - 104 2.7e-10 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07220
fragaria_vesca FvH4_2g18540
malus_domestica MD05G1171600.v1.1 MD10G1159800.v1.1
prunus_persica Prupe.8G198900_v2.0.a1
pyrus_communis pycom05g15830 pycom10g13800
rosa_chinensis RchiOBHm_Chr6g0284101
rosa_laevigata RLG00000012769
rosa_multiflora Rmu_sc0003778.1_g000044 Rmu_sc0015649.1_g000003
rosa_roxburghii Rroxscaffold_7G00184930
rosa_rugosa Rorug06G0160700.1
rosa_samantha Rh6BG274200
rosa_wichuraiana Rw6G023540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 3 cut(s) 391, 400, 432
AccII CGCG 5 cut(s) 38, 163, 175, 196, 209
AciI CCGC 9 cut(s) 5, 8, 29, 144, 147, 196, 384, 588, 741
AclWI GGATC 1 cut(s) 303
AcsI RAATTY 1 cut(s) 177
AcuI CTGAAG 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 3 cut(s) 65, 434, 884
AfiI CCNNNNNNNGG 4 cut(s) 244, 388, 884, 948
AgsI TTSAA 1 cut(s) 47
AjnI CCWGG 5 cut(s) 90, 371, 647, 745, 853
AleI CACNNNNGTG 2 cut(s) 20, 821
AloI GAACNNNNNNTCC 2 cut(s) 17, 49
AluBI AGCT 3 cut(s) 43, 283, 620
AluI AGCT 3 cut(s) 43, 283, 620
Alw21I GWGCWC 2 cut(s) 255, 729
Alw26I GTCTC 2 cut(s) 654, 982
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 807
Ama87I CYCGRG 3 cut(s) 212, 263, 819
AoxI GGCC 5 cut(s) 94, 415, 454, 851, 946
ApaI GGGCCC 1 cut(s) 419
ApeKI GCWGC 4 cut(s) 12, 234, 801, 898
ApoI RAATTY 1 cut(s) 177
ArsI GACNNNNNNTTYG 2 cut(s) 909, 941
Asp718I GGTACC 1 cut(s) 432
AspLEI GCGC 3 cut(s) 165, 175, 211
AsuC2I CCSGG 5 cut(s) 264, 265, 275, 419, 950
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 3 cut(s) 212, 263, 819
AvaII GGWCC 7 cut(s) 217, 359, 368, 645, 743, 857, 939
BaeGI GKGCMC 1 cut(s) 419
BanI GGYRCC 3 cut(s) 391, 400, 432
BanII GRGCYC 2 cut(s) 410, 419
BauI CACGAG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 255, 729
BbvI GCAGC 4 cut(s) 24, 221, 813, 885
BccI CCATC 1 cut(s) 632
BceAI ACGGC 2 cut(s) 247, 901
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 5 cut(s) 92, 373, 649, 747, 855
BcnI CCSGG 5 cut(s) 264, 265, 275, 419, 950
BcoDI GTCTC 2 cut(s) 654, 982
BglI GCCNNNNNGGC 2 cut(s) 144, 414
BisI GCNGC 8 cut(s) 6, 13, 145, 148, 197, 235, 802, 899
BlsI GCNGC 8 cut(s) 7, 14, 146, 149, 198, 236, 803, 900
BmcAI AGTACT 1 cut(s) 65
Bme18I GGWCC 7 cut(s) 217, 359, 368, 645, 743, 857, 939
BmeT110I CYCGRG 3 cut(s) 212, 263, 819
BmiI GGNNCC 7 cut(s) 269, 369, 370, 393, 402, 417, 434
BmsI GCATC 3 cut(s) 747, 813, 820
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 3 cut(s) 118, 636, 980
BpuMI CCSGG 5 cut(s) 264, 265, 275, 419, 950
BsaHI GRCGYC 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 4 cut(s) 244, 388, 884, 948
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 395, 963
BseBI CCWGG 5 cut(s) 92, 373, 649, 747, 855
BseGI GGATG 3 cut(s) 523, 706, 762
BseLI CCNNNNNNNGG 4 cut(s) 244, 388, 884, 948
BseMII CTCAG 1 cut(s) 834
BseNI ACTGG 2 cut(s) 395, 963
BseRI GAGGAG 4 cut(s) 358, 509, 512, 527
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 4 cut(s) 24, 221, 813, 885
Bsh1236I CGCG 5 cut(s) 38, 163, 175, 196, 209
BshFI GGCC 5 cut(s) 96, 417, 456, 853, 948
BshNI GGYRCC 3 cut(s) 391, 400, 432
BsiHKAI GWGCWC 2 cut(s) 255, 729
BsiHKCI CYCGRG 3 cut(s) 212, 263, 819
BsiSI CCGG 6 cut(s) 97, 264, 275, 338, 419, 949
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 4 cut(s) 244, 388, 884, 948
BsmAI GTCTC 2 cut(s) 654, 982
BsmBI CGTCTC 1 cut(s) 982
BsmFI GGGAC 1 cut(s) 354
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 5 cut(s) 96, 417, 456, 853, 948
BsoBI CYCGRG 3 cut(s) 212, 263, 819
Bsp120I GGGCCC 1 cut(s) 415
Bsp1286I GDGCHC 4 cut(s) 255, 410, 419, 729
Bsp143I GATC 4 cut(s) 295, 306, 655, 733
Bsp19I CCATGG 1 cut(s) 878
Bsp68I TCGCGA 1 cut(s) 38
BspACI CCGC 9 cut(s) 5, 8, 29, 144, 147, 196, 384, 588, 741
BspANI GGCC 5 cut(s) 96, 417, 456, 853, 948
BspCNI CTCAG 1 cut(s) 835
BspFNI CGCG 5 cut(s) 38, 163, 175, 196, 209
BspLI GGNNCC 7 cut(s) 269, 369, 370, 393, 402, 417, 434
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 3 cut(s) 391, 400, 432
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 2 cut(s) 395, 963
BssAI RCCGGY 1 cut(s) 337
BssMI GATC 4 cut(s) 295, 306, 655, 733
BssNI GRCGYC 1 cut(s) 199
BssSI CACGAG 1 cut(s) 239
BssT1I CCWWGG 2 cut(s) 878, 942
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 5 cut(s) 92, 373, 649, 747, 855
Bst4CI ACNGT 2 cut(s) 118, 724
BstACI GRCGYC 1 cut(s) 199
BstAPI GCANNNNNTGC 1 cut(s) 682
BstC8I GCNNGC 3 cut(s) 10, 454, 890
BstDEI CTNAG 2 cut(s) 843, 902
BstDSI CCRYGG 2 cut(s) 411, 878
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 3 cut(s) 523, 706, 762
BstFNI CGCG 5 cut(s) 38, 163, 175, 196, 209
BstHHI GCGC 3 cut(s) 165, 175, 211
BstKTI GATC 4 cut(s) 298, 309, 658, 736
BstMAI GTCTC 2 cut(s) 654, 982
BstMBI GATC 4 cut(s) 295, 306, 655, 733
BstMWI GCNNNNNNNGC 8 cut(s) 35, 144, 231, 289, 400, 414, 682, 898
BstNI CCWGG 5 cut(s) 92, 373, 649, 747, 855
BstNSI RCATGY 1 cut(s) 73
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 5 cut(s) 38, 163, 175, 196, 209
BstV1I GCAGC 4 cut(s) 24, 221, 813, 885
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 295
BstXI CCANNNNNNTGG 2 cut(s) 91, 753
BstYI RGATCY 1 cut(s) 295
BsuRI GGCC 5 cut(s) 96, 417, 456, 853, 948
BtgI CCRYGG 2 cut(s) 411, 878
BtsCI GGATG 3 cut(s) 523, 706, 762
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 5 cut(s) 15, 402, 538, 663, 956
BtuMI TCGCGA 1 cut(s) 38
Cac8I GCNNGC 3 cut(s) 10, 454, 890
CaiI CAGNNNCTG 1 cut(s) 807
CfoI GCGC 3 cut(s) 165, 175, 211
Cfr10I RCCGGY 1 cut(s) 337
Cfr9I CCCGGG 1 cut(s) 263
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 64, 433, 883
CviAII CATG 4 cut(s) 70, 151, 170, 879
CviQI GTAC 3 cut(s) 64, 433, 883
DdeI CTNAG 2 cut(s) 843, 902
DpnI GATC 4 cut(s) 297, 308, 657, 735
DpnII GATC 4 cut(s) 295, 306, 655, 733
Eco130I CCWWGG 2 cut(s) 878, 942
Eco24I GRGCYC 2 cut(s) 410, 419
Eco47I GGWCC 7 cut(s) 217, 359, 368, 645, 743, 857, 939
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 3 cut(s) 212, 263, 819
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoO109I RGGNCCY 2 cut(s) 217, 368
EcoRII CCWGG 5 cut(s) 90, 371, 647, 745, 853
EcoT14I CCWWGG 2 cut(s) 878, 942
EcoT38I GRGCYC 2 cut(s) 410, 419
ErhI CCWWGG 2 cut(s) 878, 942
Esp3I CGTCTC 1 cut(s) 982
FaeI CATG 4 cut(s) 73, 154, 173, 882
FaqI GGGAC 1 cut(s) 354
FatI CATG 4 cut(s) 69, 150, 169, 878
FauI CCCGC 3 cut(s) 22, 391, 581
Fnu4HI GCNGC 8 cut(s) 6, 13, 145, 148, 197, 235, 802, 899
FokI GGATG 3 cut(s) 530, 713, 769
FriOI GRGCYC 2 cut(s) 410, 419
Fsp4HI GCNGC 8 cut(s) 6, 13, 145, 148, 197, 235, 802, 899
GlaI GCGC 3 cut(s) 164, 174, 210
GluI GCNGC 8 cut(s) 6, 13, 145, 148, 197, 235, 802, 899
GsuI CTGGAG 3 cut(s) 118, 636, 980
HaeIII GGCC 5 cut(s) 96, 417, 456, 853, 948
HapII CCGG 6 cut(s) 97, 264, 275, 338, 419, 949
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 165, 175, 211
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 4 cut(s) 73, 154, 173, 882
Hin6I GCGC 3 cut(s) 163, 173, 209
HinP1I GCGC 3 cut(s) 163, 173, 209
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 3 cut(s) 204, 287, 863
HpaII CCGG 6 cut(s) 97, 264, 275, 338, 419, 949
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 2 cut(s) 121, 127
Hpy188I TCNGA 4 cut(s) 306, 311, 733, 844
Hpy188III TCNNGA 3 cut(s) 37, 659, 860
Hpy8I GTNNAC 2 cut(s) 121, 127
Hpy99I CGWCG 2 cut(s) 118, 204
HpyAV CCTTC 4 cut(s) 463, 554, 636, 822
HpyCH4III ACNGT 2 cut(s) 118, 724
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 2 cut(s) 73, 685
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 144, 231, 289, 400, 414, 682, 898
HpyF3I CTNAG 2 cut(s) 843, 902
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 4 cut(s) 73, 154, 173, 882
HspAI GCGC 3 cut(s) 163, 173, 209
KflI GGGWCCC 1 cut(s) 368
KpnI GGTACC 1 cut(s) 436
Kzo9I GATC 4 cut(s) 295, 306, 655, 733
LmnI GCTCC 4 cut(s) 258, 345, 617, 790
Lsp1109I GCAGC 4 cut(s) 24, 221, 813, 885
LweI GCATC 3 cut(s) 747, 813, 820
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 952
MalI GATC 4 cut(s) 297, 308, 657, 735
MboI GATC 4 cut(s) 295, 306, 655, 733
MboII GAAGA 7 cut(s) 65, 290, 293, 478, 556, 559, 640
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 4 cut(s) 255, 410, 419, 729
MluCI AATT 1 cut(s) 177
MlyI GAGTC 2 cut(s) 281, 872
MslI CAYNNNNRTG 2 cut(s) 20, 821
MspI CCGG 6 cut(s) 97, 264, 275, 338, 419, 949
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 5 cut(s) 92, 373, 649, 747, 855
MvnI CGCG 5 cut(s) 38, 163, 175, 196, 209
MwoI GCNNNNNNNGC 8 cut(s) 35, 144, 231, 289, 400, 414, 682, 898
NciI CCSGG 5 cut(s) 264, 265, 275, 419, 950
NcoI CCATGG 1 cut(s) 878
NdeII GATC 4 cut(s) 295, 306, 655, 733
NlaIII CATG 4 cut(s) 73, 154, 173, 882
NlaIV GGNNCC 7 cut(s) 269, 369, 370, 393, 402, 417, 434
NmeAIII GCCGAG 3 cut(s) 120, 201, 429
NruI TCGCGA 1 cut(s) 38
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 2 cut(s) 20, 821
PaeR7I CTCGAG 2 cut(s) 212, 819
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 745
PkrI GCNGC 8 cut(s) 7, 14, 146, 149, 198, 236, 803, 900
PleI GAGTC 2 cut(s) 281, 871
PpsI GAGTC 2 cut(s) 281, 871
PpuMI RGGWCCY 2 cut(s) 217, 368
Psp5II RGGWCCY 2 cut(s) 217, 368
Psp6I CCWGG 5 cut(s) 90, 371, 647, 745, 853
PspGI CCWGG 5 cut(s) 90, 371, 647, 745, 853
PspN4I GGNNCC 7 cut(s) 269, 369, 370, 393, 402, 417, 434
PspOMI GGGCCC 1 cut(s) 415
PspPPI RGGWCCY 2 cut(s) 217, 368
PspXI VCTCGAGB 2 cut(s) 212, 819
PstNI CAGNNNCTG 1 cut(s) 807
PsuI RGATCY 1 cut(s) 295
RruI TCGCGA 1 cut(s) 38
RsaI GTAC 3 cut(s) 65, 434, 884
RsaNI GTAC 3 cut(s) 64, 433, 883
RseI CAYNNNNRTG 2 cut(s) 20, 821
SatI GCNGC 8 cut(s) 6, 13, 145, 148, 197, 235, 802, 899
Sau3AI GATC 4 cut(s) 295, 306, 655, 733
ScaI AGTACT 1 cut(s) 65
SchI GAGTC 2 cut(s) 281, 872
SduI GDGCHC 4 cut(s) 255, 410, 419, 729
SfaNI GCATC 3 cut(s) 747, 813, 820
Sfr274I CTCGAG 2 cut(s) 212, 819
SinI GGWCC 7 cut(s) 217, 359, 368, 645, 743, 857, 939
SlaI CTCGAG 2 cut(s) 212, 819
SmaI CCCGGG 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 20, 821
SmlI CTYRAG 2 cut(s) 212, 819
SmoI CTYRAG 2 cut(s) 212, 819
Sse9I AATT 1 cut(s) 177
SsiI CCGC 9 cut(s) 5, 8, 29, 144, 147, 196, 384, 588, 741
StyI CCWWGG 2 cut(s) 878, 942
TaaI ACNGT 2 cut(s) 118, 724
TaiI ACGT 1 cut(s) 126
TaqI TCGA 6 cut(s) 60, 113, 202, 213, 658, 820
TasI AATT 1 cut(s) 177
TatI WGTACW 1 cut(s) 63
TauI GCSGC 4 cut(s) 8, 147, 150, 199
TfiI GAWTC 1 cut(s) 204
TscAI CASTG 5 cut(s) 22, 402, 538, 670, 963
TseI GCWGC 4 cut(s) 12, 234, 801, 898
TspDTI ATGAA 3 cut(s) 167, 764, 978
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 5 cut(s) 22, 402, 538, 670, 963
VpaK11BI GGWCC 7 cut(s) 217, 359, 368, 645, 743, 857, 939
XagI CCTNNNNNAGG 1 cut(s) 242
XapI RAATTY 1 cut(s) 177
XceI RCATGY 1 cut(s) 73
XhoI CTCGAG 2 cut(s) 212, 819
XmaI CCCGGG 1 cut(s) 263
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.