pycom10g13800

Forkhead associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015944
Physical Location & Seq
Reverse (-)
17312826 .. 17315430
2605 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g13800.2

Sequence Viewer

Length: 1170 bp
ATGGGTAGAAAGAATTACAGTGAATTTTGTGACAGAGACGAAGCAGAAGAGCGACGGAGACCAAGGTGTTCGTTTAGGGCAGGCAGGGAGCAGAGAAAATTGAGAGGACCGAACCCAATTGGGATTTGGGGGAATTATATCAGTTTCTTCTCGGATCCGCAATCTGTAAAAATTTCAATGGCGGCGGGCAGCACTGATGTGGAGGCGGGGTTCGCGAAGCTTCAGGGCGAAGACTTCGAGTACTACATGCAGACCTACTCCATAATCCTGGGCCGGAACTCCAAGAAATCGACGGTGGACGTCGACCTCTCCAGCCTCGGCGGCGGCATGAACATTTCGCGCCACCATGCGCGAATTTTCTACGACTTCACGCGGCGTCGATTCGCGCTCGAGGTCCTCGGCAAGAACGGCTGCCTCGTGGAGGGTGTGCTCCACCTACCCGGGAACCCGCCGGTCAAGCTGGACTCGCAGGATCTTCTTCAGATCGGAGATAAGGAGTTTTACTTTCTGTTGCCGGTGAGGAGCATTCTTGGTGGTCCTGTGGGTCCCAGGCATTACCCCGCCTCGGTGCCAGTGGTGCCGAGCCCCGTGGGCCCGGCTCATTACGGGTATCATTCGGGTTCGGGTGCTGGGCCGATTGGGAAGAAGGGGAGGGGGAGGGACTTGTATGAGGAGGAGTATGATGACGAGGAGGATGTTGGTGGGGGCAGTGGCAGTGGGAAGAAGATGAGGAGAGAAGGGTATGAGGGTTTTGGGTATGGTGGCGGGTCGGGTTCTGGTTCAGGCAAGGCTGGAGCTTTGGAGAAGAAAGGAGATGGAAGGTCCAGGGTTGATCGAGACACTGACAATCAGCAACTTCTGCATTTGGAGGAAAAGGATGTTGTGTCATCTGTTGCGACTGTGCTCTCCGATCTTTGCGGTCCAGGAGAATGGATGCCTATGGAGAAACTTCATAATGAGTTGGTGGAGCAGTATAGCAGCATCTGGCATCACACTCGAGTGAGGAGGTATCTCACTTCTGAGGAATGGCCTGGTCCAGAGTCAAAAGGCAAACCATGGTACGGCTTGCTTATGCTGCTAAGAAAATACCCCGAACACTTTGTCATCAACACAAGGTCCAAGGGCCGGGTAACACTGGAGTTTGTTTCTCTCGTCTCTCTGCTTTCATAA

Protein Analysis

390

Amino Acids

43.25

Weight (kDa)

8.18

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07220
fragaria_vesca FvH4_2g18540
malus_domestica MD05G1171600.v1.1 MD10G1159800.v1.1
prunus_persica Prupe.8G198900_v2.0.a1
pyrus_communis pycom05g15830 pycom10g13800
rosa_chinensis RchiOBHm_Chr6g0284101
rosa_laevigata RLG00000012769
rosa_multiflora Rmu_sc0003778.1_g000044 Rmu_sc0015649.1_g000003
rosa_roxburghii Rroxscaffold_7G00184930
rosa_rugosa Rorug06G0160700.1
rosa_samantha Rh6BG274200
rosa_wichuraiana Rw6G023540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 303
AccB1I GGYRCC 2 cut(s) 568, 577
AccI GTMKAC 1 cut(s) 303
AccII CGCG 5 cut(s) 215, 340, 352, 373, 386
AclWI GGATC 3 cut(s) 149, 162, 480
AcsI RAATTY 3 cut(s) 23, 171, 354
AcuI CTGAAG 2 cut(s) 206, 464
AcyI GRCGYC 2 cut(s) 300, 376
AfaI GTAC 2 cut(s) 242, 1061
AfiI CCNNNNNNNGG 4 cut(s) 421, 565, 1061, 1125
AgsI TTSAA 1 cut(s) 177
AjnI CCWGG 5 cut(s) 267, 548, 824, 922, 1030
AleI CACNNNNGTG 2 cut(s) 197, 998
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 3 cut(s) 220, 460, 797
AluI AGCT 3 cut(s) 220, 460, 797
Alw21I GWGCWC 2 cut(s) 432, 906
Alw26I GTCTC 4 cut(s) 30, 52, 831, 1159
AlwI GGATC 3 cut(s) 149, 162, 480
AlwNI CAGNNNCTG 1 cut(s) 984
Ama87I CYCGRG 3 cut(s) 389, 440, 996
AoxI GGCC 5 cut(s) 271, 592, 632, 1028, 1123
ApaI GGGCCC 1 cut(s) 596
ApeKI GCWGC 4 cut(s) 189, 411, 978, 1075
ApoI RAATTY 3 cut(s) 23, 171, 354
ArsI GACNNNNNNTTYG 2 cut(s) 1086, 1118
AspLEI GCGC 3 cut(s) 342, 352, 388
AsuC2I CCSGG 4 cut(s) 441, 442, 596, 1127
AsuHPI GGTGA 1 cut(s) 529
AvaI CYCGRG 3 cut(s) 389, 440, 996
AvaII GGWCC 8 cut(s) 107, 394, 536, 545, 822, 920, 1034, 1116
BaeGI GKGCMC 1 cut(s) 596
BamHI GGATCC 1 cut(s) 154
BanI GGYRCC 2 cut(s) 568, 577
BanII GRGCYC 2 cut(s) 587, 596
BauI CACGAG 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 432, 906
BbvI GCAGC 4 cut(s) 201, 398, 990, 1062
BccI CCATC 1 cut(s) 809
BceAI ACGGC 2 cut(s) 424, 1078
BcgI CGANNNNNNTGC 2 cut(s) 977, 1011
BciT130I CCWGG 5 cut(s) 269, 550, 826, 924, 1032
BcnI CCSGG 4 cut(s) 441, 442, 596, 1127
BcoDI GTCTC 4 cut(s) 30, 52, 831, 1159
BglI GCCNNNNNGGC 2 cut(s) 321, 591
BisI GCNGC 8 cut(s) 183, 190, 322, 325, 374, 412, 979, 1076
BlsI GCNGC 8 cut(s) 184, 191, 323, 326, 375, 413, 980, 1077
BmcAI AGTACT 1 cut(s) 242
Bme1390I CCNGG 9 cut(s) 269, 441, 442, 550, 596, 826, 924, 1032, 1127
Bme18I GGWCC 8 cut(s) 107, 394, 536, 545, 822, 920, 1034, 1116
BmeT110I CYCGRG 3 cut(s) 389, 440, 996
BmiI GGNNCC 7 cut(s) 156, 446, 546, 547, 570, 579, 594
BmrFI CCNGG 9 cut(s) 269, 441, 442, 550, 596, 826, 924, 1032, 1127
BmsI GCATC 3 cut(s) 924, 990, 997
BpiI GAAGAC 1 cut(s) 237
BpmI CTGGAG 3 cut(s) 295, 813, 1157
BpuMI CCSGG 4 cut(s) 441, 442, 596, 1127
BsaHI GRCGYC 2 cut(s) 300, 376
BsaI GGTCTC 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 4 cut(s) 421, 565, 1061, 1125
Bse118I RCCGGY 2 cut(s) 451, 514
Bse1I ACTGG 2 cut(s) 572, 1140
BseBI CCWGG 5 cut(s) 269, 550, 826, 924, 1032
BseGI GGATG 3 cut(s) 700, 883, 939
BseLI CCNNNNNNNGG 4 cut(s) 421, 565, 1061, 1125
BseMII CTCAG 1 cut(s) 1011
BseNI ACTGG 2 cut(s) 572, 1140
BseRI GAGGAG 6 cut(s) 535, 686, 689, 704, 745, 1018
BseSI GKGCMC 1 cut(s) 596
BseXI GCAGC 4 cut(s) 201, 398, 990, 1062
BseYI CCCAGC 1 cut(s) 629
Bsh1236I CGCG 5 cut(s) 215, 340, 352, 373, 386
BshFI GGCC 5 cut(s) 273, 594, 634, 1030, 1125
BshNI GGYRCC 2 cut(s) 568, 577
BsiHKAI GWGCWC 2 cut(s) 432, 906
BsiHKCI CYCGRG 3 cut(s) 389, 440, 996
BsiSI CCGG 6 cut(s) 274, 441, 452, 515, 596, 1126
BslFI GGGAC 2 cut(s) 531, 674
BslI CCNNNNNNNGG 4 cut(s) 421, 565, 1061, 1125
BsmAI GTCTC 4 cut(s) 30, 52, 831, 1159
BsmBI CGTCTC 2 cut(s) 30, 1159
BsmFI GGGAC 2 cut(s) 531, 674
BsmI GAATGC 1 cut(s) 525
BsnI GGCC 5 cut(s) 273, 594, 634, 1030, 1125
Bso31I GGTCTC 1 cut(s) 52
BsoBI CYCGRG 3 cut(s) 389, 440, 996
Bsp120I GGGCCC 1 cut(s) 592
Bsp1286I GDGCHC 4 cut(s) 432, 587, 596, 906
Bsp143I GATC 5 cut(s) 154, 472, 483, 832, 910
Bsp19I CCATGG 1 cut(s) 1055
Bsp68I TCGCGA 1 cut(s) 215
BspANI GGCC 5 cut(s) 273, 594, 634, 1030, 1125
BspCNI CTCAG 1 cut(s) 1012
BspFNI CGCG 5 cut(s) 215, 340, 352, 373, 386
BspLI GGNNCC 7 cut(s) 156, 446, 546, 547, 570, 579, 594
BspPI GGATC 3 cut(s) 149, 162, 480
BspQI GCTCTTC 1 cut(s) 42
BspT107I GGYRCC 2 cut(s) 568, 577
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 2 cut(s) 451, 514
BsrI ACTGG 2 cut(s) 572, 1140
BssAI RCCGGY 2 cut(s) 451, 514
BssMI GATC 5 cut(s) 154, 472, 483, 832, 910
BssNI GRCGYC 2 cut(s) 300, 376
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 3 cut(s) 62, 1055, 1119
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 5 cut(s) 269, 550, 826, 924, 1032
Bst4CI ACNGT 3 cut(s) 20, 295, 901
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 2 cut(s) 300, 376
BstAPI GCANNNNNTGC 1 cut(s) 859
BstC8I GCNNGC 3 cut(s) 82, 187, 1067
BstDEI CTNAG 2 cut(s) 1020, 1079
BstDSI CCRYGG 2 cut(s) 588, 1055
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 3 cut(s) 700, 883, 939
BstFNI CGCG 5 cut(s) 215, 340, 352, 373, 386
BstHHI GCGC 3 cut(s) 342, 352, 388
BstKTI GATC 5 cut(s) 157, 475, 486, 835, 913
BstMAI GTCTC 4 cut(s) 30, 52, 831, 1159
BstMBI GATC 5 cut(s) 154, 472, 483, 832, 910
BstMWI GCNNNNNNNGC 9 cut(s) 212, 321, 408, 457, 466, 577, 591, 859, 1075
BstNI CCWGG 5 cut(s) 269, 550, 826, 924, 1032
BstNSI RCATGY 1 cut(s) 250
BstSCI CCNGG 9 cut(s) 267, 439, 440, 548, 594, 824, 922, 1030, 1125
BstSLI GKGCMC 1 cut(s) 596
BstUI CGCG 5 cut(s) 215, 340, 352, 373, 386
BstV1I GCAGC 4 cut(s) 201, 398, 990, 1062
BstV2I GAAGAC 1 cut(s) 237
BstX2I RGATCY 2 cut(s) 154, 472
BstXI CCANNNNNNTGG 2 cut(s) 268, 930
BstYI RGATCY 2 cut(s) 154, 472
BsuRI GGCC 5 cut(s) 273, 594, 634, 1030, 1125
BtgI CCRYGG 2 cut(s) 588, 1055
BtsCI GGATG 3 cut(s) 700, 883, 939
BtsI GCAGTG 2 cut(s) 715, 721
BtsIMutI CAGTG 7 cut(s) 25, 192, 579, 715, 721, 840, 1133
BtuMI TCGCGA 1 cut(s) 215
Cac8I GCNNGC 3 cut(s) 82, 187, 1067
CaiI CAGNNNCTG 1 cut(s) 984
CfoI GCGC 3 cut(s) 342, 352, 388
Cfr10I RCCGGY 2 cut(s) 451, 514
Cfr9I CCCGGG 1 cut(s) 440
CseI GACGC 1 cut(s) 365
Csp6I GTAC 2 cut(s) 241, 1060
CviAII CATG 4 cut(s) 247, 328, 347, 1056
CviQI GTAC 2 cut(s) 241, 1060
DdeI CTNAG 2 cut(s) 1020, 1079
DpnI GATC 5 cut(s) 156, 474, 485, 834, 912
DpnII GATC 5 cut(s) 154, 472, 483, 832, 910
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 3 cut(s) 62, 1055, 1119
Eco24I GRGCYC 2 cut(s) 587, 596
Eco31I GGTCTC 1 cut(s) 52
Eco47I GGWCC 8 cut(s) 107, 394, 536, 545, 822, 920, 1034, 1116
Eco57I CTGAAG 2 cut(s) 206, 464
Eco88I CYCGRG 3 cut(s) 389, 440, 996
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoO109I RGGNCCY 2 cut(s) 394, 545
EcoRII CCWGG 5 cut(s) 267, 548, 824, 922, 1030
EcoT14I CCWWGG 3 cut(s) 62, 1055, 1119
EcoT38I GRGCYC 2 cut(s) 587, 596
ErhI CCWWGG 3 cut(s) 62, 1055, 1119
Esp3I CGTCTC 2 cut(s) 30, 1159
FaeI CATG 4 cut(s) 250, 331, 350, 1059
FaqI GGGAC 2 cut(s) 531, 674
FatI CATG 4 cut(s) 246, 327, 346, 1055
FauI CCCGC 5 cut(s) 178, 199, 456, 568, 758
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 8 cut(s) 183, 190, 322, 325, 374, 412, 979, 1076
FokI GGATG 3 cut(s) 707, 890, 946
FriOI GRGCYC 2 cut(s) 587, 596
Fsp4HI GCNGC 8 cut(s) 183, 190, 322, 325, 374, 412, 979, 1076
GlaI GCGC 3 cut(s) 341, 351, 387
GluI GCNGC 8 cut(s) 183, 190, 322, 325, 374, 412, 979, 1076
GsaI CCCAGC 1 cut(s) 633
GsuI CTGGAG 3 cut(s) 295, 813, 1157
HaeIII GGCC 5 cut(s) 273, 594, 634, 1030, 1125
HapII CCGG 6 cut(s) 274, 441, 452, 515, 596, 1126
HgaI GACGC 1 cut(s) 365
HhaI GCGC 3 cut(s) 342, 352, 388
Hin1I GRCGYC 2 cut(s) 300, 376
Hin1II CATG 4 cut(s) 250, 331, 350, 1059
Hin6I GCGC 3 cut(s) 340, 350, 386
HinP1I GCGC 3 cut(s) 340, 350, 386
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 3 cut(s) 381, 464, 1040
HpaII CCGG 6 cut(s) 274, 441, 452, 515, 596, 1126
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 2 cut(s) 298, 304
Hpy188I TCNGA 5 cut(s) 154, 483, 488, 910, 1021
Hpy188III TCNNGA 3 cut(s) 214, 836, 1037
Hpy8I GTNNAC 2 cut(s) 298, 304
Hpy99I CGWCG 4 cut(s) 57, 295, 305, 381
HpyAV CCTTC 3 cut(s) 640, 731, 813
HpyCH4III ACNGT 3 cut(s) 20, 295, 901
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 250, 862
HpyF10VI GCNNNNNNNGC 9 cut(s) 212, 321, 408, 457, 466, 577, 591, 859, 1075
HpyF3I CTNAG 2 cut(s) 1020, 1079
HpySE526I ACGT 1 cut(s) 300
Hsp92I GRCGYC 2 cut(s) 300, 376
Hsp92II CATG 4 cut(s) 250, 331, 350, 1059
HspAI GCGC 3 cut(s) 340, 350, 386
KflI GGGWCCC 1 cut(s) 545
Kzo9I GATC 5 cut(s) 154, 472, 483, 832, 910
LguI GCTCTTC 1 cut(s) 42
LmnI GCTCC 5 cut(s) 88, 435, 522, 794, 967
Lsp1109I GCAGC 4 cut(s) 201, 398, 990, 1062
LweI GCATC 3 cut(s) 924, 990, 997
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 29, 1129
MalI GATC 5 cut(s) 156, 474, 485, 834, 912
MboI GATC 5 cut(s) 154, 472, 483, 832, 910
MboII GAAGA 9 cut(s) 59, 139, 242, 467, 470, 655, 733, 736, 817
MfeI CAATTG 1 cut(s) 117
MflI RGATCY 2 cut(s) 154, 472
MhlI GDGCHC 4 cut(s) 432, 587, 596, 906
MluCI AATT 7 cut(s) 13, 23, 98, 117, 133, 171, 354
MlyI GAGTC 2 cut(s) 458, 1049
MslI CAYNNNNRTG 2 cut(s) 197, 998
MspI CCGG 6 cut(s) 274, 441, 452, 515, 596, 1126
MspR9I CCNGG 9 cut(s) 269, 441, 442, 550, 596, 826, 924, 1032, 1127
MunI CAATTG 1 cut(s) 117
Mva1269I GAATGC 1 cut(s) 525
MvaI CCWGG 5 cut(s) 269, 550, 826, 924, 1032
MvnI CGCG 5 cut(s) 215, 340, 352, 373, 386
MwoI GCNNNNNNNGC 9 cut(s) 212, 321, 408, 457, 466, 577, 591, 859, 1075
NciI CCSGG 4 cut(s) 441, 442, 596, 1127
NcoI CCATGG 1 cut(s) 1055
NdeII GATC 5 cut(s) 154, 472, 483, 832, 910
NlaIII CATG 4 cut(s) 250, 331, 350, 1059
NlaIV GGNNCC 7 cut(s) 156, 446, 546, 547, 570, 579, 594
NmeAIII GCCGAG 3 cut(s) 297, 378, 606
NmuCI GTSAC 1 cut(s) 29
NruI TCGCGA 1 cut(s) 215
NspI RCATGY 1 cut(s) 250
OliI CACNNNNGTG 2 cut(s) 197, 998
PaeR7I CTCGAG 2 cut(s) 389, 996
PciSI GCTCTTC 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PctI GAATGC 1 cut(s) 525
PfeI GAWTC 1 cut(s) 381
PfoI TCCNGGA 1 cut(s) 922
PkrI GCNGC 8 cut(s) 184, 191, 323, 326, 375, 413, 980, 1077
PleI GAGTC 2 cut(s) 458, 1048
PpsI GAGTC 2 cut(s) 458, 1048
PpuMI RGGWCCY 2 cut(s) 394, 545
Psp5II RGGWCCY 2 cut(s) 394, 545
Psp6I CCWGG 5 cut(s) 267, 548, 824, 922, 1030
PspFI CCCAGC 1 cut(s) 629
PspGI CCWGG 5 cut(s) 267, 548, 824, 922, 1030
PspN4I GGNNCC 7 cut(s) 156, 446, 546, 547, 570, 579, 594
PspOMI GGGCCC 1 cut(s) 592
PspPPI RGGWCCY 2 cut(s) 394, 545
PspXI VCTCGAGB 2 cut(s) 389, 996
PstNI CAGNNNCTG 1 cut(s) 984
PsuI RGATCY 2 cut(s) 154, 472
RruI TCGCGA 1 cut(s) 215
RsaI GTAC 2 cut(s) 242, 1061
RsaNI GTAC 2 cut(s) 241, 1060
RseI CAYNNNNRTG 2 cut(s) 197, 998
SalI GTCGAC 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 42
SatI GCNGC 8 cut(s) 183, 190, 322, 325, 374, 412, 979, 1076
Sau3AI GATC 5 cut(s) 154, 472, 483, 832, 910
ScaI AGTACT 1 cut(s) 242
SchI GAGTC 2 cut(s) 458, 1049
ScrFI CCNGG 9 cut(s) 269, 441, 442, 550, 596, 826, 924, 1032, 1127
SduI GDGCHC 4 cut(s) 432, 587, 596, 906
SfaNI GCATC 3 cut(s) 924, 990, 997
Sfr274I CTCGAG 2 cut(s) 389, 996
SinI GGWCC 8 cut(s) 107, 394, 536, 545, 822, 920, 1034, 1116
SlaI CTCGAG 2 cut(s) 389, 996
SmaI CCCGGG 1 cut(s) 442
SmiMI CAYNNNNRTG 2 cut(s) 197, 998
SmlI CTYRAG 2 cut(s) 389, 996
SmoI CTYRAG 2 cut(s) 389, 996
Sse9I AATT 7 cut(s) 13, 23, 98, 117, 133, 171, 354
StyD4I CCNGG 9 cut(s) 267, 439, 440, 548, 594, 824, 922, 1030, 1125
StyI CCWWGG 3 cut(s) 62, 1055, 1119
TaaI ACNGT 3 cut(s) 20, 295, 901
TaiI ACGT 1 cut(s) 303
TaqI TCGA 7 cut(s) 237, 290, 303, 379, 390, 835, 997
TaqII GACCGA 1 cut(s) 124
TasI AATT 7 cut(s) 13, 23, 98, 117, 133, 171, 354
TatI WGTACW 1 cut(s) 240
TauI GCSGC 4 cut(s) 185, 324, 327, 376
TfiI GAWTC 1 cut(s) 381
TscAI CASTG 7 cut(s) 25, 199, 579, 715, 721, 847, 1140
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 4 cut(s) 189, 411, 978, 1075
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 344, 941, 1155
TspGWI ACGGA 1 cut(s) 70
TspMI CCCGGG 1 cut(s) 440
TspRI CASTG 7 cut(s) 25, 199, 579, 715, 721, 847, 1140
VpaK11BI GGWCC 8 cut(s) 107, 394, 536, 545, 822, 920, 1034, 1116
XagI CCTNNNNNAGG 1 cut(s) 419
XapI RAATTY 3 cut(s) 23, 171, 354
XceI RCATGY 1 cut(s) 250
XcmI CCANNNNNNNNNTGG 1 cut(s) 123
XhoI CTCGAG 2 cut(s) 389, 996
XmaI CCCGGG 1 cut(s) 440
XmiI GTMKAC 1 cut(s) 303
ZraI GACGTC 1 cut(s) 301
ZrmI AGTACT 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.