AT3G10260

Reticulon

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006895
Physical Location & Seq
Reverse (-)
3171177 .. 3172996
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10260.3

Sequence Viewer

Length: 744 bp
ATGCCTGACAAGAACATCGTAGAGGACGTTATCGGTGATTTGGTGGATAACTTCACGGAGACTGTTCAGAAGAACAAACATGGTTCCTCCTTCTTCGAACAAGAAGATTCCGTCAGTTCTCGTTTCAACCGTTTGTTTGATCGCCAGAAGCCAATTCATCATGTCTTAGGCGGTGGCAAATCTGCTGATGTGTTGTTATGGAGGAACAAGAAGATCTCTGCAAGTGTTTTGATGGGAGCTACCGCAATTTGGGTGCTTTTTGAATGGATCAATTTCCATTTCCTGTCTCTCGTTTGCTATGCCCTCTTGCTTGGTATGATTGCACAATTCGTTTGGTCTAACGCCTCTGGATTCTTAAACCGCTCACAGTCTAGAGTGCCTCGCCTAGTTCTTCCAAAGGATTTCTTTGCTGAAGTCGGTGTGGCCGTTGGAAAAGAAGTTAACCGCGGTTTGTTGTTTCTTCAGGACTTGGCTTGTAAAGGGAATTTGAAACAGTTCCTCATGGCTGTAATCGGACTATGGGTAGCTGCAATGGTCGGGAGCTGTTGCAATTTCCTAACTGTTTTGTATATCGGGTTTGTTGGGGCACACACAATGCCGGTTCTGTATGAGAGATACGAAGACGAAGTGGATGGTTTTATGGATTCTATGATCATGAAGTTTCATAGTCATTACAAGAAGCTTGATACTGGGTTTCTCAGTAGAATCCCAAGTGGAAAGTTTGGATTAAAGAAGCGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

247

Amino Acids

27.91

Weight (kDa)

8.83

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 82 - 235 6.9e-55 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 363
AccII CGCG 1 cut(s) 447
AciI CCGC 5 cut(s) 171, 243, 361, 445, 447
AclWI GGATC 1 cut(s) 275
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 2 cut(s) 432, 446
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 3 cut(s) 127, 263, 490
AluBI AGCT 4 cut(s) 239, 527, 543, 682
AluI AGCT 4 cut(s) 239, 527, 543, 682
Alw26I GTCTC 2 cut(s) 53, 291
AlwI GGATC 1 cut(s) 275
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 1 cut(s) 484
Asp700I GAANNNNTTC 1 cut(s) 494
AsuHPI GGTGA 1 cut(s) 47
AsuII TTCGAA 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 589
BbsI GAAGAC 1 cut(s) 627
BbvI GCAGC 1 cut(s) 514
BccI CCATC 2 cut(s) 226, 626
BceAI ACGGC 1 cut(s) 410
BclI TGATCA 1 cut(s) 651
BcoDI GTCTC 2 cut(s) 53, 291
BfaI CTAG 2 cut(s) 372, 386
BglII AGATCT 1 cut(s) 213
BisI GCNGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 529
BmiI GGNNCC 1 cut(s) 85
BmrI ACTGGG 1 cut(s) 699
BmuI ACTGGG 1 cut(s) 699
BpiI GAAGAC 1 cut(s) 627
Bpu14I TTCGAA 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse118I RCCGGY 1 cut(s) 598
Bse1I ACTGG 1 cut(s) 694
Bse3DI GCAATG 1 cut(s) 537
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMI GCAATG 1 cut(s) 537
BseMII CTCAG 1 cut(s) 712
BseNI ACTGG 1 cut(s) 694
BseSI GKGCMC 1 cut(s) 589
BseXI GCAGC 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 599
BslI CCNNNNNNNGG 1 cut(s) 249
BsmAI GTCTC 2 cut(s) 53, 291
BsnI GGCC 1 cut(s) 425
Bsp119I TTCGAA 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 589
Bsp143I GATC 4 cut(s) 139, 213, 267, 651
BspACI CCGC 5 cut(s) 171, 243, 361, 445, 447
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 1 cut(s) 711
BspFNI CGCG 1 cut(s) 447
BspHI TCATGA 1 cut(s) 654
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 537
BsrFI RCCGGY 1 cut(s) 598
BsrI ACTGG 1 cut(s) 694
BssAI RCCGGY 1 cut(s) 598
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 4 cut(s) 139, 213, 267, 651
Bst4CI ACNGT 5 cut(s) 64, 131, 369, 495, 562
BstBI TTCGAA 1 cut(s) 96
BstDEI CTNAG 2 cut(s) 166, 698
BstDSI CCRYGG 1 cut(s) 445
BstF5I GGATG 1 cut(s) 637
BstFNI CGCG 1 cut(s) 447
BstKTI GATC 4 cut(s) 142, 216, 270, 654
BstMAI GTCTC 2 cut(s) 53, 291
BstMBI GATC 4 cut(s) 139, 213, 267, 651
BstSLI GKGCMC 1 cut(s) 589
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 514
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 445
BtsCI GGATG 1 cut(s) 637
CciI TCATGA 1 cut(s) 654
Cfr10I RCCGGY 1 cut(s) 598
Cfr42I CCGCGG 1 cut(s) 448
CviAII CATG 4 cut(s) 80, 161, 502, 655
CviJI RGCY 8 cut(s) 151, 239, 425, 473, 506, 527, 543, 682
CviKI_1 RGCY 8 cut(s) 151, 239, 425, 473, 506, 527, 543, 682
DdeI CTNAG 2 cut(s) 166, 698
DpnI GATC 4 cut(s) 141, 215, 269, 653
DpnII GATC 4 cut(s) 139, 213, 267, 651
EaeI YGGCCR 1 cut(s) 423
Eco57I CTGAAG 2 cut(s) 432, 446
FaeI CATG 4 cut(s) 83, 164, 505, 658
FalI AAGNNNNNCTT 2 cut(s) 389, 421
FatI CATG 4 cut(s) 79, 160, 501, 654
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 1 cut(s) 644
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 2 cut(s) 372, 386
GluI GCNGC 1 cut(s) 528
HaeIII GGCC 1 cut(s) 425
HapII CCGG 1 cut(s) 599
Hin1II CATG 4 cut(s) 83, 164, 505, 658
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 680
HinfI GANTC 4 cut(s) 107, 351, 644, 705
HpaI GTTAAC 1 cut(s) 442
HpaII CCGG 1 cut(s) 599
HphI GGTGA 1 cut(s) 47
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 2 cut(s) 69, 515
Hpy188III TCNNGA 5 cut(s) 348, 372, 464, 538, 655
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 5 cut(s) 64, 131, 369, 495, 562
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 4 cut(s) 221, 323, 530, 549
HpyF3I CTNAG 2 cut(s) 166, 698
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 4 cut(s) 83, 164, 505, 658
Ksp22I TGATCA 1 cut(s) 651
KspAI GTTAAC 1 cut(s) 442
KspI CCGCGG 1 cut(s) 448
Kzo9I GATC 4 cut(s) 139, 213, 267, 651
LmnI GCTCC 2 cut(s) 236, 540
LpnPI CCDG 7 cut(s) 18, 158, 296, 333, 449, 612, 675
Lsp1109I GCAGC 1 cut(s) 514
MaeI CTAG 2 cut(s) 372, 386
MaeII ACGT 1 cut(s) 27
MalI GATC 4 cut(s) 141, 215, 269, 653
MbiI CCGCTC 1 cut(s) 363
MboI GATC 4 cut(s) 139, 213, 267, 651
MboII GAAGA 7 cut(s) 82, 85, 116, 223, 383, 452, 632
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 589
MluCI AATT 6 cut(s) 153, 246, 271, 326, 484, 550
MmeI TCCRAC 1 cut(s) 409
MnlI CCTC 7 cut(s) 16, 97, 195, 314, 355, 390, 509
MroXI GAANNNNTTC 1 cut(s) 494
MseI TTAA 3 cut(s) 356, 441, 728
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 1 cut(s) 599
MvnI CGCG 1 cut(s) 447
NdeII GATC 4 cut(s) 139, 213, 267, 651
NlaIII CATG 4 cut(s) 83, 164, 505, 658
NlaIV GGNNCC 1 cut(s) 85
NspV TTCGAA 1 cut(s) 96
PagI TCATGA 1 cut(s) 654
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 127, 423
PdmI GAANNNNTTC 1 cut(s) 494
PfeI GAWTC 4 cut(s) 107, 351, 644, 705
PkrI GCNGC 1 cut(s) 529
PspN4I GGNNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 213
SacII CCGCGG 1 cut(s) 448
SaqAI TTAA 3 cut(s) 356, 441, 728
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 4 cut(s) 139, 213, 267, 651
SduI GDGCHC 1 cut(s) 589
SetI ASST 5 cut(s) 30, 241, 529, 545, 684
Sfr303I CCGCGG 1 cut(s) 448
SfuI TTCGAA 1 cut(s) 96
SgrBI CCGCGG 1 cut(s) 448
Sse9I AATT 6 cut(s) 153, 246, 271, 326, 484, 550
SsiI CCGC 5 cut(s) 171, 243, 361, 445, 447
SspMI CTAG 2 cut(s) 372, 386
TaaI ACNGT 5 cut(s) 64, 131, 369, 495, 562
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 96
TasI AATT 6 cut(s) 153, 246, 271, 326, 484, 550
TfiI GAWTC 4 cut(s) 107, 351, 644, 705
Tru1I TTAA 3 cut(s) 356, 441, 728
Tru9I TTAA 3 cut(s) 356, 441, 728
TseI GCWGC 1 cut(s) 527
TspDTI ATGAA 3 cut(s) 146, 653, 671
TspGWI ACGGA 2 cut(s) 71, 100
XapI RAATTY 1 cut(s) 484
XbaI TCTAGA 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 494
XspI CTAG 2 cut(s) 372, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.