pycom09g06650

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006895
Physical Location & Seq
Reverse (-)
4903167 .. 4904724
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06650.4

Sequence Viewer

Length: 741 bp
ATGCCTGAGAAAATAACTGCCGAGGATCAAGTGAACAACAACGCGGGCACACTTGCTGATACGAAACTGAAATCTGGCTCTTTATTTGGGGAAGAGACATCAAGCTCGGTCACCACTCAGTTCAATCGGCTGTTTGGACGCCAGAAACCTGTCCACCACATTTTGGGTGGAGGCAAATCTGCTGATGTCTTGTTGTGGAGGAACAAAAAGATTTCAGCTAGTGTTTTAACTGCTGCAACTGTTGTCTGGGTGCTCTTTGAATGGCTCAATTATCATTTCTTGACTCTTGTGGGGTTTGCTTTGGTTGTTGGCATGCTTGTTCAGTTCCTGTGGTCAAATTTTTCAGGCATGATTAGCAGTTCCCCATCTAAAGTACCTCGACTTGTTCTGCCCGAGGACTTATTCGTCAATATTGCCGTCTCAATTGGTGCTGAAATTAATCGAGGGTTGGCATTTGTTCAAGATGTGGCATATGGAGGAAATGTGAAGCAATTCCTTATGGTTGTAGGAAGCTTGTGGATTGCTGCTGTGATAGGAAGCTGGTTCAATTTTCTGACCATTTTGTACATTGGTTTTGTTGCTGCTCACACTTTGCCGGTTCTGTATGAGAGATACGATGATCAGGTTGACAGCTTTGTATACCAGGTCCTTGGTCTGCTTCAACACAATTACCGGAAGCTGGATGCCGGTGTCCTCAGCAAGATACCTAAAGGAAAGCTGAACTGGAAGAAGTATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

247

Amino Acids

27.33

Weight (kDa)

9.18

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 404
AccB7I CCANNNNNTGG 1 cut(s) 163
AccI GTMKAC 1 cut(s) 639
AccII CGCG 1 cut(s) 44
AciI CCGC 1 cut(s) 44
AclWI GGATC 1 cut(s) 33
AcsI RAATTY 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 2 cut(s) 375, 566
AfiI CCNNNNNNNGG 2 cut(s) 163, 679
AgsI TTSAA 5 cut(s) 124, 260, 461, 547, 662
AjnI CCWGG 1 cut(s) 642
AluBI AGCT 7 cut(s) 105, 218, 513, 540, 633, 679, 718
AluI AGCT 7 cut(s) 105, 218, 513, 540, 633, 679, 718
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 2 cut(s) 89, 424
AlwI GGATC 1 cut(s) 33
AlwNI CAGNNNCTG 1 cut(s) 328
Ama87I CYCGRG 1 cut(s) 392
ApeKI GCWGC 3 cut(s) 233, 524, 581
ApoI RAATTY 1 cut(s) 337
AseI ATTAAT 1 cut(s) 438
Asp700I GAANNNNTTC 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 646
BaeGI GKGCMC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 255
BbvCI CCTCAGC 1 cut(s) 695
BbvI GCAGC 3 cut(s) 220, 511, 568
BccI CCATC 1 cut(s) 373
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 1 cut(s) 644
BclI TGATCA 1 cut(s) 619
BcoDI GTCTC 2 cut(s) 89, 424
BfaI CTAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 234, 525, 582
BlsI GCNGC 3 cut(s) 235, 526, 583
Bme1390I CCNGG 1 cut(s) 644
Bme18I GGWCC 1 cut(s) 646
BmeT110I CYCGRG 1 cut(s) 392
BmgT120I GGNCC 1 cut(s) 646
BmrFI CCNGG 1 cut(s) 644
BmsI GCATC 1 cut(s) 673
Bpu10I CCTNAGC 1 cut(s) 695
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 3 cut(s) 21, 393, 649
BsaWI WCCGGW 1 cut(s) 672
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 679
Bse118I RCCGGY 2 cut(s) 595, 686
Bse1I ACTGG 1 cut(s) 728
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 3 cut(s) 21, 393, 649
BseGI GGATG 1 cut(s) 688
BseLI CCNNNNNNNGG 2 cut(s) 163, 679
BseMII CTCAG 2 cut(s) 131, 709
BseNI ACTGG 1 cut(s) 728
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 3 cut(s) 220, 511, 568
Bsh1236I CGCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 392
BsiSI CCGG 3 cut(s) 596, 673, 687
BslI CCNNNNNNNGG 2 cut(s) 163, 679
BsmAI GTCTC 2 cut(s) 89, 424
BsmBI CGTCTC 1 cut(s) 424
BsoBI CYCGRG 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 50, 255
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 2 cut(s) 25, 619
BspACI CCGC 1 cut(s) 44
BspCNI CTCAG 2 cut(s) 130, 708
BspFNI CGCG 1 cut(s) 44
BspPI GGATC 1 cut(s) 33
BsrFI RCCGGY 2 cut(s) 595, 686
BsrGI TGTACA 1 cut(s) 564
BsrI ACTGG 1 cut(s) 728
BssAI RCCGGY 2 cut(s) 595, 686
BssECI CCNNGG 3 cut(s) 21, 393, 649
BssMI GATC 2 cut(s) 25, 619
BssNAI GTATAC 1 cut(s) 640
BssNI GRCGYC 1 cut(s) 139
BssT1I CCWWGG 1 cut(s) 649
Bst1107I GTATAC 1 cut(s) 640
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 139
BstAUI TGTACA 1 cut(s) 564
BstC8I GCNNGC 2 cut(s) 46, 314
BstDEI CTNAG 3 cut(s) 6, 117, 695
BstEII GGTNACC 1 cut(s) 109
BstF5I GGATG 1 cut(s) 688
BstFNI CGCG 1 cut(s) 44
BstKTI GATC 2 cut(s) 28, 622
BstMAI GTCTC 2 cut(s) 89, 424
BstMBI GATC 2 cut(s) 25, 619
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 316
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 642
BstSLI GKGCMC 1 cut(s) 50
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 3 cut(s) 220, 511, 568
BstXI CCANNNNNNTGG 1 cut(s) 650
BstZ17I GTATAC 1 cut(s) 640
BtsCI GGATG 1 cut(s) 688
Cac8I GCNNGC 2 cut(s) 46, 314
CaiI CAGNNNCTG 1 cut(s) 328
Cfr10I RCCGGY 2 cut(s) 595, 686
Cfr13I GGNCC 1 cut(s) 646
CseI GACGC 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 2 cut(s) 374, 565
CviAII CATG 2 cut(s) 313, 349
CviQI GTAC 2 cut(s) 374, 565
DdeI CTNAG 3 cut(s) 6, 117, 695
DpnI GATC 2 cut(s) 27, 621
DpnII GATC 2 cut(s) 25, 619
DrdI GACNNNNNNGTC 1 cut(s) 404
DseDI GACNNNNNNGTC 1 cut(s) 404
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 646
Eco88I CYCGRG 1 cut(s) 392
Eco91I GGTNACC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 642
EcoT14I CCWWGG 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 649
Esp3I CGTCTC 1 cut(s) 424
FaeI CATG 2 cut(s) 316, 352
FaiI YATR 8 cut(s) 314, 350, 472, 474, 500, 606, 640, 735
FatI CATG 2 cut(s) 312, 348
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 619
FblI GTMKAC 1 cut(s) 639
Fnu4HI GCNGC 3 cut(s) 234, 525, 582
FokI GGATG 1 cut(s) 695
Fsp4HI GCNGC 3 cut(s) 234, 525, 582
FspBI CTAG 1 cut(s) 219
GluI GCNGC 3 cut(s) 234, 525, 582
HapII CCGG 3 cut(s) 596, 673, 687
HgaI GACGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 2 cut(s) 316, 352
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HindIII AAGCTT 1 cut(s) 511
HinfI GANTC 1 cut(s) 283
HpaII CCGG 3 cut(s) 596, 673, 687
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 4 cut(s) 34, 154, 628, 640
Hpy188I TCNGA 1 cut(s) 555
Hpy188III TCNNGA 2 cut(s) 280, 461
Hpy8I GTNNAC 4 cut(s) 34, 154, 628, 640
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 3 cut(s) 6, 117, 695
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 2 cut(s) 316, 352
Ksp22I TGATCA 1 cut(s) 619
Kzo9I GATC 2 cut(s) 25, 619
Lsp1109I GCAGC 3 cut(s) 220, 511, 568
LweI GCATC 1 cut(s) 673
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 109
MalI GATC 2 cut(s) 27, 621
MboI GATC 2 cut(s) 25, 619
MboII GAAGA 2 cut(s) 104, 739
MfeI CAATTG 1 cut(s) 423
MhlI GDGCHC 2 cut(s) 50, 255
MluCI AATT 7 cut(s) 268, 337, 423, 435, 491, 547, 667
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 8 cut(s) 16, 164, 192, 387, 388, 437, 470, 704
MroXI GAANNNNTTC 1 cut(s) 491
MseI TTAA 2 cut(s) 227, 438
MspI CCGG 3 cut(s) 596, 673, 687
MspR9I CCNGG 1 cut(s) 644
MunI CAATTG 1 cut(s) 423
MvaI CCWGG 1 cut(s) 644
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 1 cut(s) 354
NdeI CATATG 1 cut(s) 472
NdeII GATC 2 cut(s) 25, 619
NlaIII CATG 2 cut(s) 316, 352
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 316
PaeI GCATGC 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 163
PkrI GCNGC 3 cut(s) 235, 526, 583
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PpuMI RGGWCCY 1 cut(s) 646
PshBI ATTAAT 1 cut(s) 438
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 1 cut(s) 642
PspEI GGTNACC 1 cut(s) 109
PspGI CCWGG 1 cut(s) 642
PspPI GGNCC 1 cut(s) 646
PspPPI RGGWCCY 1 cut(s) 646
PstNI CAGNNNCTG 1 cut(s) 328
RsaI GTAC 2 cut(s) 375, 566
RsaNI GTAC 2 cut(s) 374, 565
SaqAI TTAA 2 cut(s) 227, 438
SatI GCNGC 3 cut(s) 234, 525, 582
Sau3AI GATC 2 cut(s) 25, 619
Sau96I GGNCC 1 cut(s) 646
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 644
SduI GDGCHC 2 cut(s) 50, 255
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 1 cut(s) 673
SinI GGWCC 1 cut(s) 646
SphI GCATGC 1 cut(s) 316
Sse9I AATT 7 cut(s) 268, 337, 423, 435, 491, 547, 667
SsiI CCGC 1 cut(s) 44
SspI AATATT 1 cut(s) 412
SspMI CTAG 1 cut(s) 219
StyD4I CCNGG 1 cut(s) 642
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 2 cut(s) 379, 442
TaqII GACCGA 1 cut(s) 97
TasI AATT 7 cut(s) 268, 337, 423, 435, 491, 547, 667
TatI WGTACW 1 cut(s) 564
Tru1I TTAA 2 cut(s) 227, 438
Tru9I TTAA 2 cut(s) 227, 438
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 3 cut(s) 233, 524, 581
Tsp45I GTSAC 1 cut(s) 109
Van91I CCANNNNNTGG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 646
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 1 cut(s) 337
XceI RCATGY 1 cut(s) 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.