RLG00000020639

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006895
Physical Location & Seq
Forward (+)
67024602 .. 67026673
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020639

Sequence Viewer

Length: 747 bp
ATGCCTGAGAAAATAACTCCTGAGGATCTTTTGAACAACATTGTGGGCACACTAGCTGAAAGTAGTTCAAAACAGAAATCTGTATCTTTTTTTGGCGATGACAAATCAGACTCTGTCAGTTCTCAGTTGAATCGGCTATTTGGACGCCAAAAACCTGTTCACCACCTTCTGGGTGGGGGAAAATCTGCTGACGTGTTGTTGTGGAGGAACAAAAAGATATCAGCTAGTGTTTTAACTGGTGCAACTTTTGTCTGGGTGCTCTTTGAATGGCTGAATTATCATTTCTTGACCCTTGTGGGTTTTGCTTTGGTTCTTGGTATGCTTGCTCAATTTCTCTGGTCAAATTTTTCGGGATTCGTTAACAGGTCCTCATCTAAAATACCTCGCCTTGTTCTACCAGAGGACTTATTCGTCAACATTGCCATCTCAATTGGTGCTGAAATTAATAGAGGGCTGGAGTTTATTCAGGATGTTGCTTGTGGAGGAAATCTGAAGCAATTTCTTGTGGTTGTAGCAAGCTTGTGGGCTGCTTCTGTGATAGGAAGCTGGTGCAACTTTCTGACAGTTCTGTACATTGGTTTCATTGCTGCTCATACATTGCCAGTTTTGTATGAGAAATATGACGATCAGATCGACAGCTTTGTGTACTCAGTCTTTGGTCAGCTTCAAAATCATTATCGCAAGCTGGATACTAGTGTCCTCAGCAGGATCCCTAAAGGAAATTTGAAGGCAAAGAAACATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

249

Amino Acids

27.62

Weight (kDa)

9.0

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 63 - 217 2.2e-55 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 169
AclWI GGATC 3 cut(s) 33, 703, 716
AcsI RAATTY 2 cut(s) 343, 721
AcuI CTGAAG 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 2 cut(s) 572, 647
AfiI CCNNNNNNNGG 1 cut(s) 169
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 6 cut(s) 34, 69, 130, 266, 668, 727
AhlI ACTAGT 1 cut(s) 692
AjiI CACGTC 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
AluBI AGCT 7 cut(s) 56, 224, 519, 546, 639, 664, 685
AluI AGCT 7 cut(s) 56, 224, 519, 546, 639, 664, 685
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 3 cut(s) 33, 703, 716
AlwNI CAGNNNCTG 1 cut(s) 113
ApeKI GCWGC 2 cut(s) 527, 587
ApoI RAATTY 2 cut(s) 343, 721
AseI ATTAAT 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 366
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 366
AxyI CCTNAGG 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 50
BaeI ACNNNNGTAYC 2 cut(s) 681, 714
BamHI GGATCC 1 cut(s) 708
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 701
BbvI GCAGC 2 cut(s) 514, 574
BccI CCATC 1 cut(s) 431
BciVI GTATCC 1 cut(s) 682
BcuI ACTAGT 1 cut(s) 692
BfaI CTAG 3 cut(s) 53, 225, 693
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 2 cut(s) 528, 588
BlsI GCNGC 2 cut(s) 529, 589
Bme18I GGWCC 1 cut(s) 366
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 1 cut(s) 710
BpmI CTGGAG 1 cut(s) 476
Bpu10I CCTNAGC 1 cut(s) 701
BsaHI GRCGYC 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 241, 602
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 3 cut(s) 417, 582, 596
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 3 cut(s) 417, 582, 596
BseMII CTCAG 4 cut(s) 12, 137, 663, 715
BseNI ACTGG 2 cut(s) 241, 602
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 2 cut(s) 514, 574
BsiHKAI GWGCWC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 50, 261
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 4 cut(s) 25, 625, 630, 708
BspCNI CTCAG 4 cut(s) 13, 136, 662, 714
BspLI GGNNCC 1 cut(s) 710
BspPI GGATC 3 cut(s) 33, 703, 716
BsrDI GCAATG 3 cut(s) 417, 582, 596
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 2 cut(s) 241, 602
BssMI GATC 4 cut(s) 25, 625, 630, 708
BssNI GRCGYC 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 565
BstACI GRCGYC 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 570
BstC8I GCNNGC 3 cut(s) 324, 517, 683
BstDEI CTNAG 5 cut(s) 6, 21, 123, 649, 701
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 4 cut(s) 28, 628, 633, 711
BstMBI GATC 4 cut(s) 25, 625, 630, 708
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 514, 574
BstX2I RGATCY 2 cut(s) 25, 708
BstYI RGATCY 2 cut(s) 25, 708
Bsu36I CCTNAGG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 682
BtgZI GCGATG 1 cut(s) 111
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 1 cut(s) 475
Cac8I GCNNGC 3 cut(s) 324, 517, 683
CaiI CAGNNNCTG 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 366
CseI GACGC 1 cut(s) 153
Csp6I GTAC 2 cut(s) 571, 646
CviAII CATG 1 cut(s) 740
CviQI GTAC 2 cut(s) 571, 646
DdeI CTNAG 5 cut(s) 6, 21, 123, 649, 701
DpnI GATC 4 cut(s) 27, 627, 632, 710
DpnII GATC 4 cut(s) 25, 625, 630, 708
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eco32I GATATC 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 366
Eco57I CTGAAG 1 cut(s) 512
Eco81I CCTNAGG 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 366
EcoRV GATATC 1 cut(s) 219
FaeI CATG 1 cut(s) 743
FaiI YATR 5 cut(s) 320, 594, 612, 621, 741
FatI CATG 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 528, 588
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 2 cut(s) 528, 588
FspBI CTAG 3 cut(s) 53, 225, 693
GluI GCNGC 2 cut(s) 528, 588
GsuI CTGGAG 1 cut(s) 476
HgaI GACGC 1 cut(s) 153
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 1 cut(s) 743
HincII GTYRAC 2 cut(s) 361, 415
HindII GTYRAC 2 cut(s) 361, 415
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 3 cut(s) 110, 130, 354
HpaI GTTAAC 1 cut(s) 361
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 4 cut(s) 160, 361, 415, 646
Hpy188I TCNGA 4 cut(s) 109, 492, 561, 630
Hpy188III TCNNGA 4 cut(s) 20, 286, 351, 467
Hpy8I GTNNAC 4 cut(s) 160, 361, 415, 646
HpyAV CCTTC 2 cut(s) 176, 721
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 242, 552
HpyF3I CTNAG 5 cut(s) 6, 21, 123, 649, 701
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 1 cut(s) 743
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 4 cut(s) 25, 625, 630, 708
Lsp1109I GCAGC 2 cut(s) 514, 574
MaeI CTAG 3 cut(s) 53, 225, 693
MaeII ACGT 1 cut(s) 192
MalI GATC 4 cut(s) 27, 627, 632, 710
MboI GATC 4 cut(s) 25, 625, 630, 708
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 2 cut(s) 25, 708
MhlI GDGCHC 2 cut(s) 50, 261
MluCI AATT 7 cut(s) 274, 329, 343, 429, 441, 497, 721
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 8 cut(s) 16, 198, 379, 393, 394, 443, 476, 710
MseI TTAA 3 cut(s) 233, 360, 444
MunI CAATTG 1 cut(s) 429
NdeII GATC 4 cut(s) 25, 625, 630, 708
NlaIII CATG 1 cut(s) 743
NlaIV GGNNCC 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 630
PfeI GAWTC 2 cut(s) 130, 354
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 2 cut(s) 529, 589
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
PpuMI RGGWCCY 1 cut(s) 366
PshBI ATTAAT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 710
PspPI GGNCC 1 cut(s) 366
PspPPI RGGWCCY 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 2 cut(s) 25, 708
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 2 cut(s) 572, 647
RsaNI GTAC 2 cut(s) 571, 646
SaqAI TTAA 3 cut(s) 233, 360, 444
SatI GCNGC 2 cut(s) 528, 588
Sau3AI GATC 4 cut(s) 25, 625, 630, 708
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 104
SduI GDGCHC 2 cut(s) 50, 261
SinI GGWCC 1 cut(s) 366
SpeI ACTAGT 1 cut(s) 692
Sse9I AATT 7 cut(s) 274, 329, 343, 429, 441, 497, 721
SspMI CTAG 3 cut(s) 53, 225, 693
TaaI ACNGT 1 cut(s) 565
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 633
TasI AATT 7 cut(s) 274, 329, 343, 429, 441, 497, 721
TatI WGTACW 2 cut(s) 570, 645
TfiI GAWTC 2 cut(s) 130, 354
Tru1I TTAA 3 cut(s) 233, 360, 444
Tru9I TTAA 3 cut(s) 233, 360, 444
TseI GCWGC 2 cut(s) 527, 587
TspDTI ATGAA 1 cut(s) 571
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 366
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 2 cut(s) 343, 721
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XspI CTAG 3 cut(s) 53, 225, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.