MD09G1146200.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0006895
Physical Location & Seq
Reverse (-)
11410375 .. 11413929
3555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1146200.v1.1.491

Sequence Viewer

Length: 744 bp
ATGCCTGAGAAAATAACTACCGAGGACCTCGTGAACAACATCGCGGGCACACTTGCTGATACGAAACTGAAATCTAGCTCTTTATTTGGGGAAGAGACATCAAGCTCGGTGACCACTCAGTCCAACCGGCTGTTTGGACGCCAGAAACCTGTCCACCACATTTTGGGTGGAGGCAAATCTGCTGATGTCTTGTTGTGGAGGAACAAAAAAATTTCAGCTAGTGTTTTAACTGCTGCAACTGTTGTCTGGGTGCTCTTTGAATGGCTCAATTATCATTTCTTGACTCTTGTGGGGTTTGCTTTGGTTGTTGGCATGCTTGTTCAGTTCCTGTGGTCAAATTTTTCAGGCATGATTAGCAGCAGTTCCCCATCTAAAGTACCTCGACTTGTTCTGCCTGAGGACTTATTCGTCAATATTGCCGTCTCAATTGGTGCTCAAATTAATCGAGGGTTGGCATTTGTTCAAGATGTGGCATATGGAGGAAATGTGAAGCAATTCCTTAAGGTTGTAGGATGCTTGTGGATTGCTGCTGTGATAGGAAGCTGGTGCAATTTGCTGACCATTTTGTACATTGGTTTTGTTGCTGCTCACACATTGCCGGTTCTGTATGAGAGATACGATGATCAGGTTGACAGCTTTGTATACCAGGTCCTCGGGCTGCTTGAACACAATTACCAGAAGCTGGATGCCGGTGTCCTCAGCAAGATACCTAAAGGAAAGCCGAACCGGAAGAAGTATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

248

Amino Acids

27.26

Weight (kDa)

9.13

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 61 - 217 3.1e-54 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 118, 407
AccB7I CCANNNNNTGG 2 cut(s) 163, 682
AccI GTMKAC 1 cut(s) 642
AccII CGCG 1 cut(s) 44
AciI CCGC 1 cut(s) 44
AcsI RAATTY 2 cut(s) 210, 337
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 2 cut(s) 378, 569
AfiI CCNNNNNNNGG 2 cut(s) 163, 682
AflII CTTAAG 1 cut(s) 500
AgsI TTSAA 3 cut(s) 260, 464, 665
AjnI CCWGG 1 cut(s) 645
AluBI AGCT 6 cut(s) 78, 105, 218, 543, 636, 682
AluI AGCT 6 cut(s) 78, 105, 218, 543, 636, 682
Alw21I GWGCWC 2 cut(s) 255, 436
Alw26I GTCTC 2 cut(s) 89, 427
AlwNI CAGNNNCTG 2 cut(s) 328, 682
Ama87I CYCGRG 1 cut(s) 653
ApeKI GCWGC 5 cut(s) 233, 357, 527, 584, 658
ApoI RAATTY 2 cut(s) 210, 337
AseI ATTAAT 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 494
AspS9I GGNCC 2 cut(s) 25, 649
AsuHPI GGTGA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 2 cut(s) 25, 649
AxyI CCTNAGG 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 50
BauI CACGAG 1 cut(s) 29
Bbv12I GWGCWC 2 cut(s) 255, 436
BbvCI CCTCAGC 1 cut(s) 698
BbvI GCAGC 5 cut(s) 220, 369, 514, 571, 645
BccI CCATC 1 cut(s) 376
BceAI ACGGC 1 cut(s) 404
BciT130I CCWGG 1 cut(s) 647
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 89, 427
BfaI CTAG 2 cut(s) 75, 219
BfrI CTTAAG 1 cut(s) 500
BisI GCNGC 5 cut(s) 234, 358, 528, 585, 659
BlsI GCNGC 5 cut(s) 235, 359, 529, 586, 660
Bme1390I CCNGG 1 cut(s) 647
Bme18I GGWCC 2 cut(s) 25, 649
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 2 cut(s) 25, 649
BmrFI CCNGG 1 cut(s) 647
BmsI GCATC 2 cut(s) 503, 676
Bpu10I CCTNAGC 1 cut(s) 698
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 2 cut(s) 21, 652
BsaWI WCCGGW 1 cut(s) 726
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 682
Bse118I RCCGGY 3 cut(s) 126, 598, 689
Bse21I CCTNAGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 593
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 2 cut(s) 21, 652
BseGI GGATG 2 cut(s) 518, 691
BseLI CCNNNNNNNGG 2 cut(s) 163, 682
BseMI GCAATG 1 cut(s) 593
BseMII CTCAG 3 cut(s) 131, 387, 712
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 5 cut(s) 220, 369, 514, 571, 645
Bsh1236I CGCG 1 cut(s) 44
BsiHKAI GWGCWC 2 cut(s) 255, 436
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 4 cut(s) 127, 599, 690, 727
BslI CCNNNNNNNGG 2 cut(s) 163, 682
BsmAI GTCTC 2 cut(s) 89, 427
BsmBI CGTCTC 1 cut(s) 427
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 3 cut(s) 50, 255, 436
Bsp1407I TGTACA 1 cut(s) 567
Bsp143I GATC 1 cut(s) 622
BspACI CCGC 1 cut(s) 44
BspCNI CTCAG 3 cut(s) 130, 388, 711
BspFNI CGCG 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 593
BsrFI RCCGGY 3 cut(s) 126, 598, 689
BsrGI TGTACA 1 cut(s) 567
BssAI RCCGGY 3 cut(s) 126, 598, 689
BssECI CCNNGG 2 cut(s) 21, 652
BssMI GATC 1 cut(s) 622
BssNAI GTATAC 1 cut(s) 643
BssNI GRCGYC 1 cut(s) 139
BssSI CACGAG 1 cut(s) 29
Bst1107I GTATAC 1 cut(s) 643
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 46, 314
BstDEI CTNAG 4 cut(s) 6, 117, 396, 698
BstEII GGTNACC 1 cut(s) 109
BstF5I GGATG 2 cut(s) 518, 691
BstFNI CGCG 1 cut(s) 44
BstKTI GATC 1 cut(s) 625
BstMAI GTCTC 2 cut(s) 89, 427
BstMBI GATC 1 cut(s) 622
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstNI CCWGG 1 cut(s) 647
BstNSI RCATGY 1 cut(s) 316
BstPI GGTNACC 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 645
BstSLI GKGCMC 1 cut(s) 50
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 5 cut(s) 220, 369, 514, 571, 645
BstZ17I GTATAC 1 cut(s) 643
Bsu36I CCTNAGG 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 2 cut(s) 518, 691
Cac8I GCNNGC 2 cut(s) 46, 314
CaiI CAGNNNCTG 2 cut(s) 328, 682
Cfr10I RCCGGY 3 cut(s) 126, 598, 689
Cfr13I GGNCC 2 cut(s) 25, 649
CseI GACGC 1 cut(s) 147
CsiI ACCWGGT 1 cut(s) 645
Csp6I GTAC 2 cut(s) 377, 568
CviAII CATG 2 cut(s) 313, 349
CviQI GTAC 2 cut(s) 377, 568
DdeI CTNAG 4 cut(s) 6, 117, 396, 698
DpnI GATC 1 cut(s) 624
DpnII GATC 1 cut(s) 622
DrdI GACNNNNNNGTC 2 cut(s) 118, 407
DseDI GACNNNNNNGTC 2 cut(s) 118, 407
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco47I GGWCC 2 cut(s) 25, 649
Eco81I CCTNAGG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 653
Eco91I GGTNACC 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 25, 649
EcoO65I GGTNACC 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 645
Esp3I CGTCTC 1 cut(s) 427
FaeI CATG 2 cut(s) 316, 352
FaiI YATR 7 cut(s) 314, 350, 475, 477, 609, 643, 738
FatI CATG 2 cut(s) 312, 348
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 475
FbaI TGATCA 1 cut(s) 622
FblI GTMKAC 1 cut(s) 642
Fnu4HI GCNGC 5 cut(s) 234, 358, 528, 585, 659
FokI GGATG 2 cut(s) 525, 698
Fsp4HI GCNGC 5 cut(s) 234, 358, 528, 585, 659
FspBI CTAG 2 cut(s) 75, 219
GluI GCNGC 5 cut(s) 234, 358, 528, 585, 659
HapII CCGG 4 cut(s) 127, 599, 690, 727
HgaI GACGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 139
Hin1II CATG 2 cut(s) 316, 352
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 1 cut(s) 283
HpaII CCGG 4 cut(s) 127, 599, 690, 727
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 4 cut(s) 34, 154, 631, 643
Hpy188III TCNNGA 3 cut(s) 31, 280, 464
Hpy8I GTNNAC 4 cut(s) 34, 154, 631, 643
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 2 cut(s) 236, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 4 cut(s) 6, 117, 396, 698
Hsp92I GRCGYC 1 cut(s) 139
Hsp92II CATG 2 cut(s) 316, 352
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 1 cut(s) 622
Lsp1109I GCAGC 5 cut(s) 220, 369, 514, 571, 645
LweI GCATC 2 cut(s) 503, 676
MabI ACCWGGT 1 cut(s) 645
MaeI CTAG 2 cut(s) 75, 219
MaeIII GTNAC 1 cut(s) 109
MalI GATC 1 cut(s) 624
MboI GATC 1 cut(s) 622
MboII GAAGA 2 cut(s) 104, 742
MfeI CAATTG 1 cut(s) 426
MhlI GDGCHC 3 cut(s) 50, 255, 436
MluCI AATT 8 cut(s) 210, 268, 337, 426, 438, 494, 550, 670
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 147
MroXI GAANNNNTTC 1 cut(s) 494
MseI TTAA 3 cut(s) 227, 441, 501
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 4 cut(s) 127, 599, 690, 727
MspR9I CCNGG 1 cut(s) 647
MunI CAATTG 1 cut(s) 426
MvaI CCWGG 1 cut(s) 647
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 1 cut(s) 354
NdeI CATATG 1 cut(s) 475
NdeII GATC 1 cut(s) 622
NlaIII CATG 2 cut(s) 316, 352
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 1 cut(s) 316
PaeI GCATGC 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 494
PflMI CCANNNNNTGG 2 cut(s) 163, 682
PkrI GCNGC 5 cut(s) 235, 359, 529, 586, 660
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PpuMI RGGWCCY 2 cut(s) 25, 649
PshBI ATTAAT 1 cut(s) 441
Psp5II RGGWCCY 2 cut(s) 25, 649
Psp6I CCWGG 1 cut(s) 645
PspEI GGTNACC 1 cut(s) 109
PspGI CCWGG 1 cut(s) 645
PspPI GGNCC 2 cut(s) 25, 649
PspPPI RGGWCCY 2 cut(s) 25, 649
PstNI CAGNNNCTG 2 cut(s) 328, 682
RsaI GTAC 2 cut(s) 378, 569
RsaNI GTAC 2 cut(s) 377, 568
SaqAI TTAA 3 cut(s) 227, 441, 501
SatI GCNGC 5 cut(s) 234, 358, 528, 585, 659
Sau3AI GATC 1 cut(s) 622
Sau96I GGNCC 2 cut(s) 25, 649
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 3 cut(s) 50, 255, 436
SexAI ACCWGGT 1 cut(s) 645
SfaNI GCATC 2 cut(s) 503, 676
SinI GGWCC 2 cut(s) 25, 649
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SphI GCATGC 1 cut(s) 316
Sse9I AATT 8 cut(s) 210, 268, 337, 426, 438, 494, 550, 670
SsiI CCGC 1 cut(s) 44
SspI AATATT 1 cut(s) 415
SspMI CTAG 2 cut(s) 75, 219
StyD4I CCNGG 1 cut(s) 645
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 2 cut(s) 382, 445
TasI AATT 8 cut(s) 210, 268, 337, 426, 438, 494, 550, 670
TatI WGTACW 1 cut(s) 567
Tru1I TTAA 3 cut(s) 227, 441, 501
Tru9I TTAA 3 cut(s) 227, 441, 501
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 5 cut(s) 233, 357, 527, 584, 658
Tsp45I GTSAC 1 cut(s) 109
Van91I CCANNNNNTGG 2 cut(s) 163, 682
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 25, 649
VspI ATTAAT 1 cut(s) 441
XapI RAATTY 2 cut(s) 210, 337
XceI RCATGY 1 cut(s) 316
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmiI GTMKAC 1 cut(s) 642
XmnI GAANNNNTTC 1 cut(s) 494
XspI CTAG 2 cut(s) 75, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.