MD17G1132800.v1.1

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006895
Physical Location & Seq
Reverse (-)
11848693 .. 11852037
3345 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1132800.v1.1.491

Sequence Viewer

Length: 747 bp
ATGCCTGAGAAAATAACTGCCGAGGATCTTTTGAACAACATTGTGGGCACACTTGCTGATAAAAAACAGAAATCTGGCTCTTTTTTCGGGGAGGAGACATCGAGCTCAGTCACTACTCAGTTCAACTTCAACCGACTGTTTGGACGCCAGAAACCTGTCCACCACATTCTGGGTGGAGGCAAATCTGCTGATGTCTTGTTGTGGAGGAACAAAAAAATTTCGGCTAGTGTTTTAACTGCTGCGACTATTGTCTGGGTGCTCTTCGAATGGCTCAATTATCATTTCTTGACTCTTGTGGGGTTTGCTTTGATTCTTGGCATGCTTGCTCAGTTTCTGTGGTCAAATTTTTCGGGCATGATTAACAGGTCCCCATCTAAAGTACCTCGACTTGTTCTGCCGAAGGACTTATTCGTCAATATTGCCATCTCAATTGGTGCTGAAATTAATCGAGGGTTGGCATTTGTTCAAGATGTGGCATGTGAAGGAAATGTGAAGCAATTTATTGTGGTTGTAGTAAGCTTGTTGATTGCTGCTATGATAGGAAGCTGGTGCAATTTTCTGACCGTTTTGTACATTGGTTTTGTTGCTGCTCACACATTGCCGGTTCTGTATGAGAGATATGAAGATCAGGTTGACAACTTTGTATATCAGGTGCTCGGGCAGCTTCAACACAATTACCGGAAGTTGGATACTGGCGTCCTCAGCAAGATCCCTAAAGGAAAGCTGAGCTGGAAGAAGTACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

249

Amino Acids

27.8

Weight (kDa)

9.37

Isoelectric Point (pI)

26.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 63 - 218 1.4e-53 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 169
AclWI GGATC 2 cut(s) 33, 703
AcsI RAATTY 2 cut(s) 216, 343
AcyI GRCGYC 2 cut(s) 145, 696
AfaI GTAC 3 cut(s) 381, 572, 740
AfiI CCNNNNNNNGG 2 cut(s) 169, 685
AgsI TTSAA 5 cut(s) 34, 124, 130, 467, 668
AluBI AGCT 6 cut(s) 105, 519, 546, 664, 724, 729
AluI AGCT 6 cut(s) 105, 519, 546, 664, 724, 729
Alw21I GWGCWC 3 cut(s) 107, 261, 657
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 2 cut(s) 33, 703
AlwNI CAGNNNCTG 1 cut(s) 334
Ama87I CYCGRG 1 cut(s) 656
ApeKI GCWGC 4 cut(s) 239, 530, 587, 661
ApoI RAATTY 2 cut(s) 216, 343
AseI ATTAAT 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 366
AsuII TTCGAA 1 cut(s) 264
AvaI CYCGRG 1 cut(s) 656
AvaII GGWCC 1 cut(s) 366
BaeGI GKGCMC 1 cut(s) 50
BaeI ACNNNNGTAYC 2 cut(s) 681, 714
BanII GRGCYC 1 cut(s) 107
Bbv12I GWGCWC 3 cut(s) 107, 261, 657
BbvCI CCTCAGC 1 cut(s) 701
BbvI GCAGC 4 cut(s) 226, 517, 574, 673
BccI CCATC 2 cut(s) 379, 431
BciVI GTATCC 1 cut(s) 682
BcoDI GTCTC 1 cut(s) 89
BfaI CTAG 1 cut(s) 225
BfuI GTATCC 1 cut(s) 682
BisI GCNGC 4 cut(s) 240, 531, 588, 662
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 4 cut(s) 241, 532, 589, 663
Bme18I GGWCC 1 cut(s) 366
BmeT110I CYCGRG 1 cut(s) 656
BmgT120I GGNCC 1 cut(s) 366
BmiI GGNNCC 1 cut(s) 368
BoxI GACNNNNGTC 1 cut(s) 248
Bpu10I CCTNAGC 1 cut(s) 701
Bpu1102I GCTNAGC 1 cut(s) 725
Bpu14I TTCGAA 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 145, 696
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 678
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 685
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 1 cut(s) 697
Bse3DI GCAATG 1 cut(s) 596
BseDI CCNNGG 1 cut(s) 21
BseLI CCNNNNNNNGG 2 cut(s) 169, 685
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 5 cut(s) 120, 131, 341, 715, 716
BseNI ACTGG 1 cut(s) 697
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 4 cut(s) 226, 517, 574, 673
BsiHKAI GWGCWC 3 cut(s) 107, 261, 657
BsiHKCI CYCGRG 1 cut(s) 656
BsiSI CCGG 2 cut(s) 602, 679
BslFI GGGAC 1 cut(s) 352
BslI CCNNNNNNNGG 2 cut(s) 169, 685
BsmAI GTCTC 1 cut(s) 89
BsmFI GGGAC 1 cut(s) 352
BsoBI CYCGRG 1 cut(s) 656
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 4 cut(s) 50, 107, 261, 657
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 3 cut(s) 25, 625, 708
Bsp1720I GCTNAGC 1 cut(s) 725
BspCNI CTCAG 5 cut(s) 119, 130, 340, 714, 717
BspLI GGNNCC 1 cut(s) 368
BspPI GGATC 2 cut(s) 33, 703
BspQI GCTCTTC 1 cut(s) 266
BspT104I TTCGAA 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 601
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 697
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 3 cut(s) 25, 625, 708
BssNI GRCGYC 2 cut(s) 145, 696
Bst4CI ACNGT 2 cut(s) 138, 565
Bst6I CTCTTC 1 cut(s) 266
BstACI GRCGYC 2 cut(s) 145, 696
BstAUI TGTACA 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 320, 324
BstDEI CTNAG 6 cut(s) 6, 106, 117, 327, 701, 725
BstKTI GATC 3 cut(s) 28, 628, 711
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 3 cut(s) 25, 625, 708
BstMWI GCNNNNNNNGC 2 cut(s) 661, 702
BstNSI RCATGY 2 cut(s) 322, 480
BstPAI GACNNNNGTC 1 cut(s) 248
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 4 cut(s) 226, 517, 574, 673
BstX2I RGATCY 2 cut(s) 25, 708
BstYI RGATCY 2 cut(s) 25, 708
BsuI GTATCC 1 cut(s) 682
Cac8I GCNNGC 2 cut(s) 320, 324
CaiI CAGNNNCTG 1 cut(s) 334
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 1 cut(s) 366
CseI GACGC 2 cut(s) 153, 685
Csp6I GTAC 3 cut(s) 380, 571, 739
CviAII CATG 3 cut(s) 319, 355, 477
CviJI RGCY 9 cut(s) 78, 105, 224, 271, 519, 546, 664, 724, 729
CviKI_1 RGCY 9 cut(s) 78, 105, 224, 271, 519, 546, 664, 724, 729
CviQI GTAC 3 cut(s) 380, 571, 739
DdeI CTNAG 6 cut(s) 6, 106, 117, 327, 701, 725
DpnI GATC 3 cut(s) 27, 627, 710
DpnII GATC 3 cut(s) 25, 625, 708
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 1 cut(s) 266
EarI CTCTTC 1 cut(s) 266
Ecl136II GAGCTC 1 cut(s) 105
Eco24I GRGCYC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 656
EcoICRI GAGCTC 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 107
FaeI CATG 3 cut(s) 322, 358, 480
FaiI YATR 7 cut(s) 320, 356, 478, 536, 612, 621, 646
FaqI GGGAC 1 cut(s) 352
FatI CATG 3 cut(s) 318, 354, 476
Fnu4HI GCNGC 4 cut(s) 240, 531, 588, 662
FriOI GRGCYC 1 cut(s) 107
Fsp4HI GCNGC 4 cut(s) 240, 531, 588, 662
FspBI CTAG 1 cut(s) 225
GluI GCNGC 4 cut(s) 240, 531, 588, 662
HapII CCGG 2 cut(s) 602, 679
HgaI GACGC 2 cut(s) 153, 685
Hin1I GRCGYC 2 cut(s) 145, 696
Hin1II CATG 3 cut(s) 322, 358, 480
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 2 cut(s) 289, 310
HpaII CCGG 2 cut(s) 602, 679
Hpy166II GTNNAC 2 cut(s) 160, 634
Hpy188I TCNGA 1 cut(s) 561
Hpy188III TCNNGA 2 cut(s) 286, 467
Hpy8I GTNNAC 2 cut(s) 160, 634
HpyAV CCTTC 2 cut(s) 394, 476
HpyCH4III ACNGT 2 cut(s) 138, 565
HpyCH4V TGCA 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 2 cut(s) 661, 702
HpyF3I CTNAG 6 cut(s) 6, 106, 117, 327, 701, 725
Hsp92I GRCGYC 2 cut(s) 145, 696
Hsp92II CATG 3 cut(s) 322, 358, 480
Kzo9I GATC 3 cut(s) 25, 625, 708
LguI GCTCTTC 1 cut(s) 266
Lsp1109I GCAGC 4 cut(s) 226, 517, 574, 673
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 1 cut(s) 109
MalI GATC 3 cut(s) 27, 627, 710
MboI GATC 3 cut(s) 25, 625, 708
MboII GAAGA 3 cut(s) 253, 635, 745
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 2 cut(s) 25, 708
MhlI GDGCHC 4 cut(s) 50, 107, 261, 657
MluCI AATT 8 cut(s) 216, 274, 343, 429, 441, 497, 553, 673
MlyI GAGTC 1 cut(s) 283
MmeI TCCRAC 1 cut(s) 666
MnlI CCTC 7 cut(s) 16, 85, 170, 198, 393, 443, 710
MseI TTAA 3 cut(s) 233, 360, 444
MspI CCGG 2 cut(s) 602, 679
MunI CAATTG 1 cut(s) 429
MwoI GCNNNNNNNGC 2 cut(s) 661, 702
NdeII GATC 3 cut(s) 25, 625, 708
NlaIII CATG 3 cut(s) 322, 358, 480
NlaIV GGNNCC 1 cut(s) 368
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 109
NspI RCATGY 2 cut(s) 322, 480
NspV TTCGAA 1 cut(s) 264
PaeI GCATGC 1 cut(s) 322
PciSI GCTCTTC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 310
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 4 cut(s) 241, 532, 589, 663
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PpuMI RGGWCCY 1 cut(s) 366
PshAI GACNNNNGTC 1 cut(s) 248
PshBI ATTAAT 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 107
Psp5II RGGWCCY 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 368
PspPI GGNCC 1 cut(s) 366
PspPPI RGGWCCY 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 2 cut(s) 25, 708
RsaI GTAC 3 cut(s) 381, 572, 740
RsaNI GTAC 3 cut(s) 380, 571, 739
SacI GAGCTC 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 266
SaqAI TTAA 3 cut(s) 233, 360, 444
SatI GCNGC 4 cut(s) 240, 531, 588, 662
Sau3AI GATC 3 cut(s) 25, 625, 708
Sau96I GGNCC 1 cut(s) 366
SchI GAGTC 1 cut(s) 283
SduI GDGCHC 4 cut(s) 50, 107, 261, 657
SfuI TTCGAA 1 cut(s) 264
SinI GGWCC 1 cut(s) 366
SphI GCATGC 1 cut(s) 322
Sse9I AATT 8 cut(s) 216, 274, 343, 429, 441, 497, 553, 673
SspI AATATT 1 cut(s) 418
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 107
TaaI ACNGT 2 cut(s) 138, 565
TaqI TCGA 4 cut(s) 101, 264, 385, 448
TasI AATT 8 cut(s) 216, 274, 343, 429, 441, 497, 553, 673
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 3 cut(s) 233, 360, 444
Tru9I TTAA 3 cut(s) 233, 360, 444
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 4 cut(s) 239, 530, 587, 661
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 636
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 1 cut(s) 366
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 2 cut(s) 216, 343
XceI RCATGY 2 cut(s) 322, 480
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.