Rh2CG481200

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0006895
Physical Location & Seq
Forward (+)
64589944 .. 64594466
4523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG481200.1

Sequence Viewer

Length: 621 bp
ATGCCTGAGAAAATAACTGCTGAGGATCTTTTGAACAACATTGTGGGCACAATAGCTGAAAGTAGTTCAAAACAGAAATCTGTGTCTTTTTTTGGCGACGACAAATCAGACTCTGTCGGTTCTCAGTTGAATCGGCTATTTGGACGCCAAAAACCTGTTCACCACCTTCTGGGTGGAGGAAAATCTGCTGACGTGTTGTTGTGGAGGAACAAAAAGATATCAGCTAGTGTTTTAACTGGTGCAACTTTTGTTTGGGTGCTCTTTGAATGGCTGAATTATCATTTCTTGACTCTTGTGGGTTTTGCTTTGGTCCTTGGAATGCTTGCTCAATTTCTCTGGTCAAATTTTTCGGGATTGGTTAACAGGTCCTCATCAAAAATACCTCGCCTTGTTCTACCAGAGGACTTATTCGTCAACATTGCCATCTCAATTGGTGCTGAAATTAATAGAGGGCTGGAGTTTATTCAGGATGTTGCTTGTGGAGGAAATCTGAAGCAATTTCTTGTGGTTGTAGCAAGCTTGTGGGCTGCTTCAGTGATAGGAAGCTGGTGCAACTTTCTGACAGTTCTGTACATTGGTGAGTTTCCAATCGCCCCATTACCAAGAATGCAACTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Reticulon

Protein Analysis

206

Amino Acids

22.59

Weight (kDa)

8.6

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 63 - 194 5.2e-43 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 169
AclWI GGATC 1 cut(s) 33
AcsI RAATTY 1 cut(s) 343
AcuI CTGAAG 2 cut(s) 512, 516
AcyI GRCGYC 1 cut(s) 145
AfaI GTAC 1 cut(s) 572
AfiI CCNNNNNNNGG 1 cut(s) 169
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 4 cut(s) 34, 69, 130, 266
AjiI CACGTC 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 141, 173
AluBI AGCT 4 cut(s) 56, 224, 519, 546
AluI AGCT 4 cut(s) 56, 224, 519, 546
Alw21I GWGCWC 1 cut(s) 261
AlwI GGATC 1 cut(s) 33
AlwNI CAGNNNCTG 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 527
ApoI RAATTY 1 cut(s) 343
AseI ATTAAT 1 cut(s) 444
AspS9I GGNCC 2 cut(s) 310, 366
AsuHPI GGTGA 2 cut(s) 152, 590
AvaII GGWCC 2 cut(s) 310, 366
BaeGI GKGCMC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 21
BbvI GCAGC 1 cut(s) 514
BccI CCATC 1 cut(s) 431
BfaI CTAG 1 cut(s) 225
BisI GCNGC 1 cut(s) 528
BlsI GCNGC 1 cut(s) 529
Bme18I GGWCC 2 cut(s) 310, 366
BmgBI CACGTC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 310, 366
BpmI CTGGAG 1 cut(s) 476
Bpu10I CCTNAGC 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 313
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 417
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 2 cut(s) 12, 137
BseNI ACTGG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 261
BslI CCNNNNNNNGG 1 cut(s) 169
BsmI GAATGC 2 cut(s) 324, 612
Bsp1286I GDGCHC 2 cut(s) 50, 261
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 1 cut(s) 25
BspCNI CTCAG 2 cut(s) 13, 136
BspPI GGATC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 417
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 1 cut(s) 25
BssNI GRCGYC 1 cut(s) 145
BssT1I CCWWGG 1 cut(s) 313
Bst4CI ACNGT 1 cut(s) 565
BstACI GRCGYC 1 cut(s) 145
BstAUI TGTACA 1 cut(s) 570
BstC8I GCNNGC 2 cut(s) 324, 517
BstDEI CTNAG 3 cut(s) 6, 21, 123
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 1 cut(s) 28
BstMBI GATC 1 cut(s) 25
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 514
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BtrI CACGTC 1 cut(s) 193
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 540
Cac8I GCNNGC 2 cut(s) 324, 517
CaiI CAGNNNCTG 1 cut(s) 113
Cfr13I GGNCC 2 cut(s) 310, 366
CseI GACGC 1 cut(s) 153
Csp6I GTAC 1 cut(s) 571
CviJI RGCY 8 cut(s) 56, 136, 224, 271, 454, 519, 527, 546
CviKI_1 RGCY 8 cut(s) 56, 136, 224, 271, 454, 519, 527, 546
CviQI GTAC 1 cut(s) 571
DdeI CTNAG 3 cut(s) 6, 21, 123
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 313
Eco32I GATATC 1 cut(s) 219
Eco47I GGWCC 2 cut(s) 310, 366
Eco57I CTGAAG 2 cut(s) 512, 516
EcoO109I RGGNCCY 1 cut(s) 366
EcoRV GATATC 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 313
Fnu4HI GCNGC 1 cut(s) 528
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 1 cut(s) 528
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 476
HgaI GACGC 1 cut(s) 153
Hin1I GRCGYC 1 cut(s) 145
HincII GTYRAC 2 cut(s) 361, 415
HindII GTYRAC 2 cut(s) 361, 415
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 3 cut(s) 110, 130, 289
HpaI GTTAAC 1 cut(s) 361
HphI GGTGA 2 cut(s) 152, 590
Hpy166II GTNNAC 3 cut(s) 160, 361, 415
Hpy188I TCNGA 3 cut(s) 109, 492, 561
Hpy188III TCNNGA 3 cut(s) 286, 351, 467
Hpy8I GTNNAC 3 cut(s) 160, 361, 415
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 176
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 3 cut(s) 242, 552, 610
HpyF3I CTNAG 3 cut(s) 6, 21, 123
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 1 cut(s) 145
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 1 cut(s) 25
Lsp1109I GCAGC 1 cut(s) 514
MaeI CTAG 1 cut(s) 225
MaeII ACGT 1 cut(s) 192
MalI GATC 1 cut(s) 27
MboI GATC 1 cut(s) 25
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 2 cut(s) 50, 261
MluCI AATT 6 cut(s) 274, 329, 343, 429, 441, 497
MlyI GAGTC 2 cut(s) 104, 283
MnlI CCTC 8 cut(s) 16, 170, 198, 379, 393, 394, 443, 476
MseI TTAA 3 cut(s) 233, 360, 444
MunI CAATTG 1 cut(s) 429
Mva1269I GAATGC 2 cut(s) 324, 612
NdeII GATC 1 cut(s) 25
PctI GAATGC 2 cut(s) 324, 612
PfeI GAWTC 1 cut(s) 130
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 169
PkrI GCNGC 1 cut(s) 529
PleI GAGTC 2 cut(s) 104, 283
PpsI GAGTC 2 cut(s) 104, 283
PpuMI RGGWCCY 1 cut(s) 366
PshBI ATTAAT 1 cut(s) 444
Psp5II RGGWCCY 1 cut(s) 366
PspPI GGNCC 2 cut(s) 310, 366
PspPPI RGGWCCY 1 cut(s) 366
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 25
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 1 cut(s) 572
RsaNI GTAC 1 cut(s) 571
SaqAI TTAA 3 cut(s) 233, 360, 444
SatI GCNGC 1 cut(s) 528
Sau3AI GATC 1 cut(s) 25
Sau96I GGNCC 2 cut(s) 310, 366
SchI GAGTC 2 cut(s) 104, 283
SduI GDGCHC 2 cut(s) 50, 261
SetI ASST 9 cut(s) 58, 157, 168, 195, 226, 368, 385, 521, 548
SinI GGWCC 2 cut(s) 310, 366
Sse9I AATT 6 cut(s) 274, 329, 343, 429, 441, 497
SspMI CTAG 1 cut(s) 225
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 565
TaiI ACGT 1 cut(s) 195
TasI AATT 6 cut(s) 274, 329, 343, 429, 441, 497
TatI WGTACW 1 cut(s) 570
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 3 cut(s) 233, 360, 444
Tru9I TTAA 3 cut(s) 233, 360, 444
TscAI CASTG 1 cut(s) 540
TseI GCWGC 1 cut(s) 527
TspRI CASTG 1 cut(s) 540
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 1 cut(s) 169
VpaK11BI GGWCC 2 cut(s) 310, 366
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 1 cut(s) 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.