AT3G11400

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004689
Physical Location & Seq
Forward (+)
3578234 .. 3580569
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G11400.2

Sequence Viewer

Length: 885 bp
ATGACGATCGATTCGCAGCAAAAAACGAGCAAATTCAGATGGGGGGAAATGGACGAAGACGAAGATCTGGATTTCTTGCTCCCTCCCAAGCAGGTGATTGGTCCAGATGAGAATGGTTTGAAGACGACGATCGAGTACAAATTCAACGATGAAGAAAATAAGGTTAAGATAACGACTAGGACACGTGTCCGCAAGCTAGCTTCTGCTCGACTCAACAAACGAGCCATGGAGAGGAGGAACTGGCCTAAGTTCGGAGACGCAGCCAATGAGGAAGCTGGTAGCCATCTCACTATGGTTTCTACCGAAGAGATCCTTCTCGAACGACCCCGAGCTCCTGGTACCAAAGCGGATGAATCAAAGGCAACAGGAGATGGCTTGTCTCAGCTGGGTAAAGGTGGTGCAGTTCTCATGGTGTGCAGGATATGCCACAAGAAAGGAGATCACTGGACATCAAAATGTCCATACAAGGATCTAGCTGCACCAACCGATGTCTTCATTGACAAGCCACCCACAGGGGAATCATCTACCATGTCTGCTGCACCTGGAACTGGCAAGGCCGCGTATGTCCCGCCTAGCATGAGAGCAGGTGCAGACAGAAGCGCTGTGGGTTCAGACATGAGGAGGAGGAACGATGAGAACTCTGTGCGTGTAACCAATCTGTCCGAGGATACCCGTGAACCTGACTTGATGGAGCTGTTCCACCCGTTTGGAGCTGTCACACGTGTCTATGTGGCCATTGATCAGAAAACTGGAGTGAGCAGAGGATTTGGTTTCGTCAATTTCGTGAGCAGAGAAGACGCTCAAAGAGCAATCAACAAATTGAATGGTTATGGTTATGATAACCTCATCCTCAGGGTTGAATGGGCAACTCCAAGACCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
RRM_1 eIF3g

Protein Analysis

294

Amino Acids

32.71

Weight (kDa)

8.32

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF3g PF12353 31 - 185 7.8e-30 Eukaryotic translation initiation factor 3 subunit G
RRM_1 PF00076 242 - 308 4.6e-19 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 82, 575
Acc36I ACCTGC 2 cut(s) 82, 575
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 338
AccII CGCG 1 cut(s) 560
AciI CCGC 4 cut(s) 190, 347, 558, 569
AclWI GGATC 2 cut(s) 304, 477
AcoI YGGCCR 1 cut(s) 732
AcsI RAATTY 2 cut(s) 32, 140
AcvI CACGTG 2 cut(s) 185, 722
AfaI GTAC 2 cut(s) 137, 340
AfeI AGCGCT 1 cut(s) 601
AfiI CCNNNNNNNGG 4 cut(s) 231, 251, 512, 548
AflIII ACRYGT 4 cut(s) 182, 184, 719, 721
AgsI TTSAA 4 cut(s) 121, 145, 823, 860
AjnI CCWGG 2 cut(s) 334, 541
AluBI AGCT 8 cut(s) 196, 200, 275, 332, 385, 476, 694, 713
AluI AGCT 8 cut(s) 196, 200, 275, 332, 385, 476, 694, 713
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 2 cut(s) 249, 384
AlwI GGATC 2 cut(s) 304, 477
Ama87I CYCGRG 1 cut(s) 327
Aor51HI AGCGCT 1 cut(s) 601
AoxI GGCC 3 cut(s) 242, 555, 732
ApeKI GCWGC 4 cut(s) 16, 260, 476, 536
ApoI RAATTY 2 cut(s) 32, 140
ArsI GACNNNNNNTTYG 1 cut(s) 27
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 1 cut(s) 602
AspS9I GGNCC 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 106
AsuNHI GCTAGC 1 cut(s) 196
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 1 cut(s) 101
AxyI CCTNAGG 1 cut(s) 851
BalI TGGCCA 1 cut(s) 734
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 334
BbrPI CACGTG 2 cut(s) 185, 722
BbsI GAAGAC 4 cut(s) 63, 128, 484, 801
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 4 cut(s) 28, 272, 463, 523
BccI CCATC 4 cut(s) 33, 291, 365, 682
BciT130I CCWGG 2 cut(s) 336, 543
BciVI GTATCC 1 cut(s) 661
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 2 cut(s) 249, 384
BfaI CTAG 5 cut(s) 177, 197, 473, 573, 883
BfoI RGCGCY 1 cut(s) 603
BfuAI ACCTGC 2 cut(s) 82, 575
BfuI GTATCC 1 cut(s) 661
BglII AGATCT 1 cut(s) 64
BisI GCNGC 5 cut(s) 17, 261, 477, 537, 558
BlsI GCNGC 5 cut(s) 18, 262, 478, 538, 559
Bme1390I CCNGG 2 cut(s) 336, 543
Bme18I GGWCC 1 cut(s) 101
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 336, 543
BmtI GCTAGC 1 cut(s) 200
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 4 cut(s) 63, 128, 484, 801
BpmI CTGGAG 1 cut(s) 771
Bsa29I ATCGAT 1 cut(s) 9
BsaAI YACGTR 2 cut(s) 185, 722
BsaJI CCNNGG 2 cut(s) 225, 663
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 251, 512, 548
Bse1I ACTGG 4 cut(s) 245, 449, 553, 754
Bse21I CCTNAGG 1 cut(s) 851
BseBI CCWGG 2 cut(s) 336, 543
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 225, 663
BseGI GGATG 2 cut(s) 355, 846
BseLI CCNNNNNNNGG 4 cut(s) 231, 251, 512, 548
BseMII CTCAG 2 cut(s) 395, 865
BseNI ACTGG 4 cut(s) 245, 449, 553, 754
BseRI GAGGAG 3 cut(s) 247, 634, 637
BseXI GCAGC 4 cut(s) 28, 272, 463, 523
BseYI CCCAGC 1 cut(s) 385
BsgI GTGCAG 5 cut(s) 420, 436, 462, 522, 609
Bsh1236I CGCG 1 cut(s) 560
Bsh1285I CGRYCG 2 cut(s) 9, 132
BshFI GGCC 3 cut(s) 244, 557, 734
BshNI GGYRCC 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 2 cut(s) 9, 132
BsiHKAI GWGCWC 1 cut(s) 334
BsiHKCI CYCGRG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 4 cut(s) 231, 251, 512, 548
BsmAI GTCTC 2 cut(s) 249, 384
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 3 cut(s) 244, 557, 734
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 4 cut(s) 190, 347, 558, 569
BspANI GGCC 3 cut(s) 244, 557, 734
BspCNI CTCAG 2 cut(s) 394, 864
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 1 cut(s) 560
BspLI GGNNCC 1 cut(s) 340
BspMI ACCTGC 2 cut(s) 82, 575
BspOI GCTAGC 1 cut(s) 200
BspPI GGATC 2 cut(s) 304, 477
BspT107I GGYRCC 1 cut(s) 338
BsrI ACTGG 4 cut(s) 245, 449, 553, 754
BssECI CCNNGG 2 cut(s) 225, 663
BssMI GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 336, 543
Bst6I CTCTTC 1 cut(s) 300
BstAPI GCANNNNNTGC 1 cut(s) 423
BstBAI YACGTR 2 cut(s) 185, 722
BstC8I GCNNGC 2 cut(s) 194, 198
BstDEI CTNAG 3 cut(s) 246, 381, 851
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 2 cut(s) 355, 846
BstFNI CGCG 1 cut(s) 560
BstH2I RGCGCY 1 cut(s) 603
BstHHI GCGC 1 cut(s) 602
BstKTI GATC 7 cut(s) 9, 67, 132, 312, 442, 472, 742
BstMAI GTCTC 2 cut(s) 249, 384
BstMBI GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
BstMCI CGRYCG 2 cut(s) 9, 132
BstMWI GCNNNNNNNGC 2 cut(s) 423, 806
BstNI CCWGG 2 cut(s) 336, 543
BstPAI GACNNNNGTC 1 cut(s) 185
BstSCI CCNGG 2 cut(s) 334, 541
BstUI CGCG 1 cut(s) 560
BstV1I GCAGC 4 cut(s) 28, 272, 463, 523
BstV2I GAAGAC 4 cut(s) 63, 128, 484, 801
BstX2I RGATCY 3 cut(s) 64, 309, 469
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 3 cut(s) 64, 309, 469
Bsu15I ATCGAT 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 851
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 3 cut(s) 244, 557, 734
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 2 cut(s) 355, 846
BtsIMutI CAGTG 1 cut(s) 442
BveI ACCTGC 2 cut(s) 82, 575
Cac8I GCNNGC 2 cut(s) 194, 198
CfoI GCGC 1 cut(s) 602
Cfr13I GGNCC 1 cut(s) 101
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 2 cut(s) 266, 806
Csp6I GTAC 2 cut(s) 136, 339
CviAII CATG 5 cut(s) 226, 409, 529, 577, 616
CviQI GTAC 2 cut(s) 136, 339
DdeI CTNAG 3 cut(s) 246, 381, 851
DpnI GATC 7 cut(s) 8, 66, 131, 311, 441, 471, 741
DpnII GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
EaeI YGGCCR 1 cut(s) 732
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 101
Eco47III AGCGCT 1 cut(s) 601
Eco53kI GAGCTC 1 cut(s) 332
Eco72I CACGTG 2 cut(s) 185, 722
Eco81I CCTNAGG 1 cut(s) 851
Eco88I CYCGRG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 334, 541
EcoT14I CCWWGG 1 cut(s) 225
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 249
FaeI CATG 5 cut(s) 229, 412, 532, 580, 619
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FaqI GGGAC 1 cut(s) 551
FatI CATG 5 cut(s) 225, 408, 528, 576, 615
FauI CCCGC 1 cut(s) 576
FbaI TGATCA 1 cut(s) 739
Fnu4HI GCNGC 5 cut(s) 17, 261, 477, 537, 558
FokI GGATG 2 cut(s) 362, 833
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 5 cut(s) 17, 261, 477, 537, 558
FspBI CTAG 5 cut(s) 177, 197, 473, 573, 883
GlaI GCGC 1 cut(s) 601
GluI GCNGC 5 cut(s) 17, 261, 477, 537, 558
GsaI CCCAGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 771
HaeII RGCGCY 1 cut(s) 603
HaeIII GGCC 3 cut(s) 244, 557, 734
HgaI GACGC 2 cut(s) 266, 806
HhaI GCGC 1 cut(s) 602
Hin1II CATG 5 cut(s) 229, 412, 532, 580, 619
Hin6I GCGC 1 cut(s) 600
HinP1I GCGC 1 cut(s) 600
HinfI GANTC 4 cut(s) 11, 210, 353, 518
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 1 cut(s) 677
Hpy188I TCNGA 5 cut(s) 38, 254, 613, 664, 744
Hpy188III TCNNGA 4 cut(s) 68, 104, 317, 784
Hpy8I GTNNAC 1 cut(s) 677
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 1 cut(s) 323
HpyCH4IV ACGT 2 cut(s) 184, 721
HpyCH4V TGCA 5 cut(s) 401, 417, 479, 539, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 423, 806
HpyF3I CTNAG 3 cut(s) 246, 381, 851
HpySE526I ACGT 2 cut(s) 184, 721
Hsp92II CATG 5 cut(s) 229, 412, 532, 580, 619
HspAI GCGC 1 cut(s) 600
KpnI GGTACC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
LmnI GCTCC 4 cut(s) 84, 337, 691, 710
Lsp1109I GCAGC 4 cut(s) 28, 272, 463, 523
MaeI CTAG 5 cut(s) 177, 197, 473, 573, 883
MaeII ACGT 2 cut(s) 184, 721
MaeIII GTNAC 2 cut(s) 649, 715
MalI GATC 7 cut(s) 8, 66, 131, 311, 441, 471, 741
MboI GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
MboII GAAGA 7 cut(s) 68, 74, 133, 164, 317, 484, 806
MflI RGATCY 3 cut(s) 64, 309, 469
MhlI GDGCHC 1 cut(s) 334
MlsI TGGCCA 1 cut(s) 734
MluCI AATT 4 cut(s) 32, 140, 778, 818
MluNI TGGCCA 1 cut(s) 734
MlyI GAGTC 1 cut(s) 204
Mox20I TGGCCA 1 cut(s) 734
MscI TGGCCA 1 cut(s) 734
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 454
Msp20I TGGCCA 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 385
MspR9I CCNGG 2 cut(s) 336, 543
MvaI CCWGG 2 cut(s) 336, 543
MvnI CGCG 1 cut(s) 560
MwoI GCNNNNNNNGC 2 cut(s) 423, 806
NcoI CCATGG 1 cut(s) 225
NdeII GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 5 cut(s) 229, 412, 532, 580, 619
NlaIV GGNNCC 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 715
PaqCI CACCTGC 2 cut(s) 82, 575
PcsI WCGNNNNNNNCGW 1 cut(s) 780
PfeI GAWTC 3 cut(s) 11, 353, 518
PkrI GCNGC 5 cut(s) 18, 262, 478, 538, 559
Ple19I CGATCG 2 cut(s) 9, 132
PleI GAGTC 1 cut(s) 204
PmaCI CACGTG 2 cut(s) 185, 722
PmlI CACGTG 2 cut(s) 185, 722
PpsI GAGTC 1 cut(s) 204
Ppu21I YACGTR 2 cut(s) 185, 722
PshAI GACNNNNGTC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 334, 541
PspCI CACGTG 2 cut(s) 185, 722
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 2 cut(s) 334, 541
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 101
PsuI RGATCY 3 cut(s) 64, 309, 469
PvuI CGATCG 2 cut(s) 9, 132
PvuII CAGCTG 1 cut(s) 385
RsaI GTAC 2 cut(s) 137, 340
RsaNI GTAC 2 cut(s) 136, 339
RseI CAYNNNNRTG 1 cut(s) 454
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 5 cut(s) 17, 261, 477, 537, 558
Sau3AI GATC 7 cut(s) 6, 64, 129, 309, 439, 469, 739
Sau96I GGNCC 1 cut(s) 101
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 2 cut(s) 336, 543
SduI GDGCHC 1 cut(s) 334
SinI GGWCC 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 454
Sse9I AATT 4 cut(s) 32, 140, 778, 818
SsiI CCGC 4 cut(s) 190, 347, 558, 569
SspMI CTAG 5 cut(s) 177, 197, 473, 573, 883
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 2 cut(s) 334, 541
StyI CCWWGG 1 cut(s) 225
TaiI ACGT 2 cut(s) 187, 724
TaqI TCGA 4 cut(s) 9, 132, 208, 318
TasI AATT 4 cut(s) 32, 140, 778, 818
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 560
TfiI GAWTC 3 cut(s) 11, 353, 518
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 449
TseFI GTSAC 1 cut(s) 715
TseI GCWGC 4 cut(s) 16, 260, 476, 536
Tsp45I GTSAC 1 cut(s) 715
TspDTI ATGAA 3 cut(s) 165, 366, 484
TspRI CASTG 1 cut(s) 449
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 2 cut(s) 32, 140
XspI CTAG 5 cut(s) 177, 197, 473, 573, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.