Rorug05G0378500

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000519
Physical Location & Seq
Reverse (-)
51319061 .. 51328269
9209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0378500.1

Sequence Viewer

Length: 1080 bp
ATGGGCCTGCATAACCTACCATTTAAAGTTGGACAGGACATAGAATCGAGATCCTTTCTGAAAGGTTATCGTGGTGCGTGGTTCCGATGTAAGATAAAAGATATTGGCTGGAGAAATGGTCAGCTGTCGTGTGCTTTGGAATATTATGACTTTCCAGATGAAAAAATAACTTGGGAGAAGATATATCAAAACAACCCTGCTGATGGTAAAAGGACAAGAAAGGAGAAGACATTGATGCTGCGTCCTCCCTTTCCTCCTATCTATCGTGAAAGTAAAAGGCCTGATGTCAATACCATATTGGAAGTGATAGTTATTGTCAATGATGCCTGGAAAGTGGGAGATTTAGTTGATTGGTGGAAGGATGGTTGTTATTGGTCTGGAAAAGTAGCAGAAGTATTAGGGGATGAAACAGTGAAGGTTGAGTTGCCACGTCCTCCTGTTGGTGAAGGAGTTGAAGGAGAGATGTATGAGGCTTCTTACAAGGACCTGCGCTCATCCTTGGATTGGTCTCTAGAATATGGATGGACAGTACCTACACCCAAGGAGAGAGAAAATGGGCATCCTTGTGTTCAAGTAATTAAGCCTGATAATCAAGGAGGCATTCCAAGCGTGGTGGCCCAAACTGTAGGTGATGGAAGAAGAGATGTACAGGCAATTGCTGGTGCAGATCAATCTTTAGCAGCAAAGGACTTAGCAGCAAAGGACCCAGCCACTGCAGAAACAAATATGGCGTCTGATGTAGCAGATAGCCTTTTTGGGAAGACGAGTTGCTCAGATAGTGGTTCAAGTTCGCATGTGAAAGATGCATTAAATAAAATGGGGGGAACTGCAACTAGGATAGACATTGTTGATAACGATAGGTCCTTGAAGAAGATGAAAACTGACGAAGGCATAATATTGAATTCGATGAGCTCTGATACATTAGAAGCTGCAATTTTGGACTTGGAGGAACTTGTAAACCGGGTTAAATGGATGAAAGGTATGTTGGAGACTGGAATGCCTTTGACAGGTGCCATGCGCCCCCATTGGAAATTTCTTGGAAATCATACCGTGTTTACGCCAAGTGAAGGTTTTCACTAG

Protein Analysis

359

Amino Acids

40.01

Weight (kDa)

5.55

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 11 - 85 1e-08 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 1010
AclWI GGATC 1 cut(s) 45
AcsI RAATTY 2 cut(s) 901, 1031
AcyI GRCGYC 1 cut(s) 731
AfaI GTAC 2 cut(s) 531, 648
AfiI CCNNNNNNNGG 5 cut(s) 203, 440, 504, 1007, 1067
AgsI TTSAA 5 cut(s) 455, 572, 786, 868, 901
AjiI CACGTC 1 cut(s) 431
AjnI CCWGG 1 cut(s) 326
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AluBI AGCT 3 cut(s) 124, 912, 929
AluI AGCT 3 cut(s) 124, 912, 929
Alw21I GWGCWC 1 cut(s) 914
Alw26I GTCTC 2 cut(s) 513, 983
AlwI GGATC 1 cut(s) 45
AlwNI CAGNNNCTG 1 cut(s) 713
AoxI GGCC 3 cut(s) 4, 278, 615
ApeKI GCWGC 4 cut(s) 238, 680, 695, 929
ApoI RAATTY 2 cut(s) 901, 1031
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
Asp700I GAANNNNTTC 1 cut(s) 1071
AspLEI GCGC 2 cut(s) 492, 1020
AspS9I GGNCC 5 cut(s) 4, 484, 616, 703, 861
AsuC2I CCSGG 1 cut(s) 962
AsuHPI GGTGA 2 cut(s) 455, 641
AvaII GGWCC 3 cut(s) 484, 703, 861
BanI GGYRCC 1 cut(s) 1010
BanII GRGCYC 1 cut(s) 914
BbsI GAAGAC 2 cut(s) 233, 767
Bbv12I GWGCWC 1 cut(s) 914
BbvI GCAGC 4 cut(s) 225, 692, 707, 916
BccI CCATC 4 cut(s) 197, 356, 516, 626
BciT130I CCWGG 1 cut(s) 328
BcnI CCSGG 1 cut(s) 962
BcoDI GTCTC 2 cut(s) 513, 983
BfaI CTAG 3 cut(s) 512, 834, 1078
BfmI CTRYAG 2 cut(s) 624, 714
BfuAI ACCTGC 1 cut(s) 495
BisI GCNGC 4 cut(s) 239, 681, 696, 930
BlsI GCNGC 4 cut(s) 240, 682, 697, 931
Bme1390I CCNGG 2 cut(s) 328, 962
Bme18I GGWCC 3 cut(s) 484, 703, 861
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 5 cut(s) 4, 484, 616, 703, 861
BmiI GGNNCC 3 cut(s) 83, 705, 1012
BmrFI CCNGG 2 cut(s) 328, 962
BmsI GCATC 4 cut(s) 225, 313, 568, 793
BpiI GAAGAC 2 cut(s) 233, 767
BpmI CTGGAG 1 cut(s) 130
BpuMI CCSGG 1 cut(s) 962
BsaHI GRCGYC 1 cut(s) 731
BsaI GGTCTC 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 498, 540
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 330, 360
Bsc4I CCNNNNNNNGG 5 cut(s) 203, 440, 504, 1007, 1067
Bse1I ACTGG 1 cut(s) 997
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 2 cut(s) 498, 540
BseGI GGATG 6 cut(s) 367, 409, 494, 527, 559, 978
BseLI CCNNNNNNNGG 5 cut(s) 203, 440, 504, 1007, 1067
BseMII CTCAG 1 cut(s) 786
BseNI ACTGG 1 cut(s) 997
BseXI GCAGC 4 cut(s) 225, 692, 707, 916
BseYI CCCAGC 1 cut(s) 706
BsgI GTGCAG 1 cut(s) 684
BshFI GGCC 3 cut(s) 6, 280, 617
BshNI GGYRCC 1 cut(s) 1010
BsiHKAI GWGCWC 1 cut(s) 914
BsiSI CCGG 1 cut(s) 961
BslI CCNNNNNNNGG 5 cut(s) 203, 440, 504, 1007, 1067
BsmAI GTCTC 2 cut(s) 513, 983
BsmI GAATGC 2 cut(s) 600, 1002
BsnI GGCC 3 cut(s) 6, 280, 617
Bso31I GGTCTC 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 914
Bsp1407I TGTACA 1 cut(s) 646
Bsp143I GATC 2 cut(s) 50, 667
BspANI GGCC 3 cut(s) 6, 280, 617
BspCNI CTCAG 1 cut(s) 785
BspLI GGNNCC 3 cut(s) 83, 705, 1012
BspMAI CTGCAG 1 cut(s) 718
BspMI ACCTGC 1 cut(s) 495
BspPI GGATC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 1010
BspTNI GGTCTC 1 cut(s) 513
BsrGI TGTACA 1 cut(s) 646
BsrI ACTGG 1 cut(s) 997
BssECI CCNNGG 2 cut(s) 498, 540
BssMI GATC 2 cut(s) 50, 667
BssNI GRCGYC 1 cut(s) 731
BssT1I CCWWGG 2 cut(s) 498, 540
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 4 cut(s) 412, 529, 625, 1051
Bst6I CTCTTC 1 cut(s) 634
BstACI GRCGYC 1 cut(s) 731
BstAUI TGTACA 1 cut(s) 646
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 2 cut(s) 691, 772
BstENI CCTNNNNNAGG 1 cut(s) 1005
BstF5I GGATG 6 cut(s) 367, 409, 494, 527, 559, 978
BstHHI GCGC 2 cut(s) 492, 1020
BstKTI GATC 2 cut(s) 53, 670
BstMAI GTCTC 2 cut(s) 513, 983
BstMBI GATC 2 cut(s) 50, 667
BstMWI GCNNNNNNNGC 1 cut(s) 606
BstNI CCWGG 1 cut(s) 328
BstNSI RCATGY 1 cut(s) 797
BstSCI CCNGG 2 cut(s) 326, 960
BstSFI CTRYAG 2 cut(s) 624, 714
BstV1I GCAGC 4 cut(s) 225, 692, 707, 916
BstV2I GAAGAC 2 cut(s) 233, 767
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 3 cut(s) 6, 280, 617
BtrI CACGTC 1 cut(s) 431
BtsCI GGATG 6 cut(s) 367, 409, 494, 527, 559, 978
BtsI GCAGTG 1 cut(s) 711
BtsIMutI CAGTG 2 cut(s) 417, 711
BveI ACCTGC 1 cut(s) 495
Cac8I GCNNGC 1 cut(s) 8
CaiI CAGNNNCTG 1 cut(s) 713
CfoI GCGC 2 cut(s) 492, 1020
Cfr13I GGNCC 5 cut(s) 4, 484, 616, 703, 861
CseI GACGC 2 cut(s) 230, 720
Csp6I GTAC 2 cut(s) 530, 647
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 2 cut(s) 794, 1015
CviQI GTAC 2 cut(s) 530, 647
DdeI CTNAG 2 cut(s) 691, 772
DpnI GATC 2 cut(s) 52, 669
DpnII GATC 2 cut(s) 50, 667
DraI TTTAAA 1 cut(s) 25
Eam1104I CTCTTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 634
Ecl136II GAGCTC 1 cut(s) 912
Eco130I CCWWGG 2 cut(s) 498, 540
Eco147I AGGCCT 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 914
Eco31I GGTCTC 1 cut(s) 513
Eco47I GGWCC 3 cut(s) 484, 703, 861
Eco53kI GAGCTC 1 cut(s) 912
EcoICRI GAGCTC 1 cut(s) 912
EcoNI CCTNNNNNAGG 1 cut(s) 1005
EcoO109I RGGNCCY 3 cut(s) 484, 703, 861
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 498, 540
EcoT22I ATGCAT 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 914
ErhI CCWWGG 2 cut(s) 498, 540
FaeI CATG 2 cut(s) 797, 1018
FatI CATG 2 cut(s) 793, 1014
Fnu4HI GCNGC 4 cut(s) 239, 681, 696, 930
FokI GGATG 6 cut(s) 374, 416, 481, 534, 546, 985
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 4 cut(s) 239, 681, 696, 930
FspBI CTAG 3 cut(s) 512, 834, 1078
GlaI GCGC 2 cut(s) 491, 1019
GluI GCNGC 4 cut(s) 239, 681, 696, 930
GsaI CCCAGC 1 cut(s) 710
GsuI CTGGAG 1 cut(s) 130
HaeIII GGCC 3 cut(s) 6, 280, 617
HapII CCGG 1 cut(s) 961
HgaI GACGC 2 cut(s) 230, 720
HhaI GCGC 2 cut(s) 492, 1020
Hin1I GRCGYC 1 cut(s) 731
Hin1II CATG 2 cut(s) 797, 1018
Hin6I GCGC 2 cut(s) 490, 1018
HinP1I GCGC 2 cut(s) 490, 1018
HinfI GANTC 1 cut(s) 44
HpaII CCGG 1 cut(s) 961
HphI GGTGA 2 cut(s) 455, 641
Hpy166II GTNNAC 2 cut(s) 958, 1056
Hpy188I TCNGA 5 cut(s) 60, 86, 736, 775, 916
Hpy188III TCNNGA 5 cut(s) 48, 155, 266, 378, 512
Hpy8I GTNNAC 2 cut(s) 958, 1056
HpyAV CCTTC 6 cut(s) 352, 409, 440, 449, 881, 1061
HpyCH4III ACNGT 4 cut(s) 412, 529, 625, 1051
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 6 cut(s) 10, 665, 716, 806, 830, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 606
HpyF3I CTNAG 2 cut(s) 691, 772
HpySE526I ACGT 1 cut(s) 430
Hsp92I GRCGYC 1 cut(s) 731
Hsp92II CATG 2 cut(s) 797, 1018
HspAI GCGC 2 cut(s) 490, 1018
Kzo9I GATC 2 cut(s) 50, 667
Lsp1109I GCAGC 4 cut(s) 225, 692, 707, 916
LweI GCATC 4 cut(s) 225, 313, 568, 793
MaeI CTAG 3 cut(s) 512, 834, 1078
MaeII ACGT 1 cut(s) 430
MalI GATC 2 cut(s) 52, 669
MboI GATC 2 cut(s) 50, 667
MboII GAAGA 7 cut(s) 190, 238, 648, 651, 772, 880, 883
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 1 cut(s) 50
MhlI GDGCHC 1 cut(s) 914
MluCI AATT 5 cut(s) 576, 654, 901, 933, 1031
MmeI TCCRAC 2 cut(s) 10, 966
MnlI CCTC 6 cut(s) 255, 264, 444, 463, 590, 940
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 1071
MseI TTAA 4 cut(s) 24, 579, 809, 966
MslI CAYNNNNRTG 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 124
MspI CCGG 1 cut(s) 961
MspR9I CCNGG 2 cut(s) 328, 962
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 2 cut(s) 600, 1002
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 1 cut(s) 606
NciI CCSGG 1 cut(s) 962
NdeII GATC 2 cut(s) 50, 667
NlaIII CATG 2 cut(s) 797, 1018
NlaIV GGNNCC 3 cut(s) 83, 705, 1012
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 1 cut(s) 797
PceI AGGCCT 1 cut(s) 280
PctI GAATGC 2 cut(s) 600, 1002
PdmI GAANNNNTTC 1 cut(s) 1071
PfeI GAWTC 1 cut(s) 44
PkrI GCNGC 4 cut(s) 240, 682, 697, 931
PpuMI RGGWCCY 3 cut(s) 484, 703, 861
Psp124BI GAGCTC 1 cut(s) 914
Psp5II RGGWCCY 3 cut(s) 484, 703, 861
Psp6I CCWGG 1 cut(s) 326
PspFI CCCAGC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 3 cut(s) 83, 705, 1012
PspPI GGNCC 5 cut(s) 4, 484, 616, 703, 861
PspPPI RGGWCCY 3 cut(s) 484, 703, 861
PstI CTGCAG 1 cut(s) 718
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 50
PvuII CAGCTG 1 cut(s) 124
RsaI GTAC 2 cut(s) 531, 648
RsaNI GTAC 2 cut(s) 530, 647
RseI CAYNNNNRTG 1 cut(s) 564
SacI GAGCTC 1 cut(s) 914
SaqAI TTAA 4 cut(s) 24, 579, 809, 966
SatI GCNGC 4 cut(s) 239, 681, 696, 930
Sau3AI GATC 2 cut(s) 50, 667
Sau96I GGNCC 5 cut(s) 4, 484, 616, 703, 861
ScrFI CCNGG 2 cut(s) 328, 962
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 4 cut(s) 225, 313, 568, 793
SfcI CTRYAG 2 cut(s) 624, 714
SinI GGWCC 3 cut(s) 484, 703, 861
SmiMI CAYNNNNRTG 1 cut(s) 564
Sse9I AATT 5 cut(s) 576, 654, 901, 933, 1031
SseBI AGGCCT 1 cut(s) 280
SspI AATATT 2 cut(s) 143, 897
SspMI CTAG 3 cut(s) 512, 834, 1078
SstI GAGCTC 1 cut(s) 914
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 326, 960
StyI CCWWGG 2 cut(s) 498, 540
TaaI ACNGT 4 cut(s) 412, 529, 625, 1051
TaiI ACGT 1 cut(s) 433
TaqI TCGA 2 cut(s) 47, 905
TasI AATT 5 cut(s) 576, 654, 901, 933, 1031
TatI WGTACW 1 cut(s) 646
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 4 cut(s) 24, 579, 809, 966
Tru9I TTAA 4 cut(s) 24, 579, 809, 966
TscAI CASTG 2 cut(s) 417, 718
TseI GCWGC 4 cut(s) 238, 680, 695, 929
TspDTI ATGAA 4 cut(s) 174, 420, 890, 989
TspRI CASTG 2 cut(s) 417, 718
VpaK11BI GGWCC 3 cut(s) 484, 703, 861
XagI CCTNNNNNAGG 1 cut(s) 1005
XapI RAATTY 2 cut(s) 901, 1031
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 1 cut(s) 797
XmnI GAANNNNTTC 1 cut(s) 1071
XspI CTAG 3 cut(s) 512, 834, 1078
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.