Rorug05G0160400

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000519
Physical Location & Seq
Forward (+)
14931544 .. 14932318
775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0160400.1

Sequence Viewer

Length: 651 bp
ATGGGTGAAAACCATTTAACAGGTCCTATCCCTCCAAATTTTGGAAGCTTAAAAGAGCTAACTTCTTTGTTCTTGAACAACAATAATCTTTCTGGTTCAATCCCGACGTCTTTAGGTGATCTGAGAAACCTTACCTCGCTCTATCTGTATGACAACAAACTTTCTGGCACCATTCCAAAAGAGATAGGGAACTTGAAATCTATGGTCGATCTAGAGTTGAGCACAAATCAACTCAGTGGTTCCATTCCTACTTCATTTGGTGACTTGACAAACCTAGAAATCTTATTTCTTAATGATAACCAACTCTCTGGTTCCATCCCCCAAGAAATAGGGAATCTCATGAAGTTGACCATAGTGGAATTGGATAATAACCAATTTTCTGGTTATTTGCCCCAAAATATTTGCCGAGGTGGATCACTGGAAAACTTTACAGTATCCACCAACTATTTGACAGACCTAAGCCACAACAACTTATATGGTGAGATCTCACCTGTCTGGGGACAATGTCCACAATTAGCAACCCTCCGAATTGCGGGGAACAACCTTACTGGCAGCATCCCACCTGAAATTGGCAATGCAACGCAAATTCAGGTGCTCGATCTTTCATCAAATCGTTTAGTCGGGGTGATTCCTAAAGAGATTGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

216

Amino Acids

23.32

Weight (kDa)

4.5

Isoelectric Point (pI)

21.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 10 - 97 2e-08 Leucine-rich repeat region
LRR_8 PF13855 11 - 54 4e-06 Leucine rich repeat
LRR_8 PF13855 19 - 78 4.6e-07 Leucine rich repeat
LRR_8 PF13855 70 - 126 1.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 1 cut(s) 533
AclWI GGATC 1 cut(s) 421
AcsI RAATTY 2 cut(s) 37, 585
AcyI GRCGYC 1 cut(s) 107
AfiI CCNNNNNNNGG 4 cut(s) 41, 497, 532, 569
AgsI TTSAA 3 cut(s) 76, 99, 196
AluBI AGCT 2 cut(s) 48, 58
AluI AGCT 2 cut(s) 48, 58
Alw21I GWGCWC 2 cut(s) 224, 597
AlwI GGATC 1 cut(s) 421
ApeKI GCWGC 1 cut(s) 552
ApoI RAATTY 2 cut(s) 37, 585
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 6 cut(s) 17, 128, 272, 480, 491, 637
AvaII GGWCC 1 cut(s) 23
BanI GGYRCC 1 cut(s) 167
Bbv12I GWGCWC 2 cut(s) 224, 597
BbvI GCAGC 1 cut(s) 564
BccI CCATC 1 cut(s) 323
BciVI GTATCC 1 cut(s) 445
BfaI CTAG 2 cut(s) 212, 275
BfuI GTATCC 1 cut(s) 445
BglII AGATCT 1 cut(s) 483
BisI GCNGC 1 cut(s) 553
BlsI GCNGC 1 cut(s) 554
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 3 cut(s) 169, 241, 313
BmsI GCATC 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 458
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 406
Bsc4I CCNNNNNNNGG 4 cut(s) 41, 497, 532, 569
Bse1I ACTGG 2 cut(s) 423, 553
Bse3DI GCAATG 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 406
BseGI GGATG 2 cut(s) 315, 555
BseLI CCNNNNNNNGG 4 cut(s) 41, 497, 532, 569
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 2 cut(s) 113, 247
BseNI ACTGG 2 cut(s) 423, 553
BseXI GCAGC 1 cut(s) 564
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 2 cut(s) 224, 597
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 4 cut(s) 41, 497, 532, 569
BsmFI GGGAC 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 224, 597
Bsp143I GATC 5 cut(s) 118, 208, 413, 483, 598
BspACI CCGC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 114, 246
BspHI TCATGA 1 cut(s) 339
BspLI GGNNCC 3 cut(s) 169, 241, 313
BspPI GGATC 1 cut(s) 421
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 580
BsrI ACTGG 2 cut(s) 423, 553
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 5 cut(s) 118, 208, 413, 483, 598
BssNI GRCGYC 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 107
BstDEI CTNAG 3 cut(s) 122, 233, 458
BstF5I GGATG 2 cut(s) 315, 555
BstKTI GATC 5 cut(s) 121, 211, 416, 486, 601
BstMBI GATC 5 cut(s) 118, 208, 413, 483, 598
BstV1I GCAGC 1 cut(s) 564
BstX2I RGATCY 1 cut(s) 483
BstXI CCANNNNNNTGG 2 cut(s) 308, 380
BstYI RGATCY 1 cut(s) 483
BsuI GTATCC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 315, 555
BtsIMutI CAGTG 2 cut(s) 241, 416
CciI TCATGA 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 23
CviAII CATG 1 cut(s) 340
CviJI RGCY 3 cut(s) 48, 58, 462
CviKI_1 RGCY 3 cut(s) 48, 58, 462
DdeI CTNAG 3 cut(s) 122, 233, 458
DpnI GATC 5 cut(s) 120, 210, 415, 485, 600
DpnII GATC 5 cut(s) 118, 208, 413, 483, 598
Eco47I GGWCC 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 23
FaeI CATG 1 cut(s) 343
FaiI YATR 6 cut(s) 150, 203, 341, 353, 475, 477
FaqI GGGAC 1 cut(s) 513
FatI CATG 1 cut(s) 339
FauI CCCGC 1 cut(s) 526
Fnu4HI GCNGC 1 cut(s) 553
FokI GGATG 2 cut(s) 302, 542
Fsp4HI GCNGC 1 cut(s) 553
FspBI CTAG 2 cut(s) 212, 275
GluI GCNGC 1 cut(s) 553
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 1 cut(s) 343
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 46
HinfI GANTC 2 cut(s) 334, 628
HphI GGTGA 6 cut(s) 17, 128, 272, 480, 491, 637
Hpy166II GTNNAC 2 cut(s) 348, 509
Hpy188I TCNGA 2 cut(s) 123, 527
Hpy188III TCNNGA 4 cut(s) 73, 103, 212, 340
Hpy8I GTNNAC 2 cut(s) 348, 509
Hpy99I CGWCG 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 1 cut(s) 107
HpyCH4V TGCA 1 cut(s) 578
HpyF3I CTNAG 3 cut(s) 122, 233, 458
HpySE526I ACGT 1 cut(s) 107
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 1 cut(s) 343
Kzo9I GATC 5 cut(s) 118, 208, 413, 483, 598
Lsp1109I GCAGC 1 cut(s) 564
LweI GCATC 1 cut(s) 564
MaeI CTAG 2 cut(s) 212, 275
MaeII ACGT 1 cut(s) 107
MaeIII GTNAC 1 cut(s) 260
MalI GATC 5 cut(s) 120, 210, 415, 485, 600
MboI GATC 5 cut(s) 118, 208, 413, 483, 598
MflI RGATCY 1 cut(s) 483
MhlI GDGCHC 2 cut(s) 224, 597
MluCI AATT 7 cut(s) 37, 359, 374, 512, 528, 567, 585
MnlI CCTC 4 cut(s) 42, 145, 401, 533
MseI TTAA 3 cut(s) 17, 50, 291
NdeII GATC 5 cut(s) 118, 208, 413, 483, 598
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 3 cut(s) 169, 241, 313
NmeAIII GCCGAG 1 cut(s) 431
NmuCI GTSAC 1 cut(s) 260
PagI TCATGA 1 cut(s) 339
PfeI GAWTC 2 cut(s) 334, 628
PflFI GACNNNGTC 1 cut(s) 504
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 1 cut(s) 554
PpuMI RGGWCCY 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 23
PspN4I GGNNCC 3 cut(s) 169, 241, 313
PspPI GGNCC 1 cut(s) 23
PspPPI RGGWCCY 1 cut(s) 23
PsuI RGATCY 1 cut(s) 483
PsyI GACNNNGTC 1 cut(s) 504
SaqAI TTAA 3 cut(s) 17, 50, 291
SatI GCNGC 1 cut(s) 553
Sau3AI GATC 5 cut(s) 118, 208, 413, 483, 598
Sau96I GGNCC 1 cut(s) 23
SduI GDGCHC 2 cut(s) 224, 597
SfaNI GCATC 1 cut(s) 564
SinI GGWCC 1 cut(s) 23
Sse9I AATT 7 cut(s) 37, 359, 374, 512, 528, 567, 585
SsiI CCGC 1 cut(s) 533
SspI AATATT 1 cut(s) 400
SspMI CTAG 2 cut(s) 212, 275
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 1 cut(s) 110
TaqI TCGA 2 cut(s) 207, 597
TasI AATT 7 cut(s) 37, 359, 374, 512, 528, 567, 585
TfiI GAWTC 2 cut(s) 334, 628
Tru1I TTAA 3 cut(s) 17, 50, 291
Tru9I TTAA 3 cut(s) 17, 50, 291
TscAI CASTG 2 cut(s) 241, 423
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 1 cut(s) 552
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 3 cut(s) 243, 356, 594
TspRI CASTG 2 cut(s) 241, 423
Tth111I GACNNNGTC 1 cut(s) 504
Van91I CCANNNNNTGG 1 cut(s) 41
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 2 cut(s) 37, 585
XbaI TCTAGA 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 358
XspI CTAG 2 cut(s) 212, 275
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.