Rorug03G0256300

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000519
Physical Location & Seq
Forward (+)
24409682 .. 24410490
809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0256300.1

Sequence Viewer

Length: 594 bp
ATGGAACCACAACAACAGCAACCAACAGAGGAGGGAGGAAACACCCAAGCAGCTGGAAGTAGTGCCAACATGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGAACAAATAAGAATCCTCAAGGATCTTTACTATGTCAAGGGATTTAAGTGCCCAACTACAGAGCAGATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGGCATGTTGAGGGTAAGAACATTTACTTTTGGTTCCAGAACGTCAGGGCTCGAGAGAAGCAGATGAATAGGTGTAATCAGGCTGCTCCAGTGCCCATGAGAACTAGTTCTCTTGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGATGATGGAGGACAAACATCCCTGCAACAACGAGGAGGAGATCACCAGGAGGTTCAAACTCTTCCTCTGTTCCCCGTGCACGGCGAGGACGTCTTTGGTAACCTGAAGGCTACTTCCGAGGAAGGTAGCGCTTTTGGTTACTATTCTGGTGGCTCAGGCGGTTACCACAGTGGCTCAAACGTTTCTCTTGAGCTCAGCCTCAACCCATCCGGAGCTACCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

197

Amino Acids

21.64

Weight (kDa)

5.51

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 30 - 91 6.2e-10 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
AccIII TCCGGA 1 cut(s) 578
AciI CCGC 1 cut(s) 528
AclI AACGTT 1 cut(s) 549
AclWI GGATC 3 cut(s) 141, 327, 340
AcuI CTGAAG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 1 cut(s) 91
AfeI AGCGCT 1 cut(s) 499
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 1 cut(s) 425
AhlI ACTAGT 2 cut(s) 314, 359
AjnI CCWGG 2 cut(s) 92, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 4 cut(s) 53, 199, 562, 584
AluI AGCT 4 cut(s) 53, 199, 562, 584
Alw21I GWGCWC 2 cut(s) 450, 564
Alw26I GTCTC 1 cut(s) 197
Alw44I GTGCAC 1 cut(s) 446
AlwI GGATC 3 cut(s) 141, 327, 340
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 578
Aor51HI AGCGCT 1 cut(s) 499
ApaLI GTGCAC 1 cut(s) 446
ApeKI GCWGC 2 cut(s) 50, 293
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
Asp700I GAANNNNTTC 1 cut(s) 316
AspLEI GCGC 1 cut(s) 500
AspS9I GGNCC 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 354
BaeGI GKGCMC 3 cut(s) 164, 306, 450
BamHI GGATCC 1 cut(s) 332
BanII GRGCYC 2 cut(s) 262, 564
BauI CACGAG 1 cut(s) 182
Bbv12I GWGCWC 2 cut(s) 450, 564
BbvI GCAGC 2 cut(s) 62, 280
BccI CCATC 2 cut(s) 368, 583
BceAI ACGGC 2 cut(s) 72, 466
BciT130I CCWGG 2 cut(s) 94, 416
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 2 cut(s) 314, 359
BfaI CTAG 2 cut(s) 315, 360
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 501
BglII AGATCT 1 cut(s) 186
BisI GCNGC 2 cut(s) 51, 294
BlpI GCTNAGC 1 cut(s) 563
BlsI GCNGC 2 cut(s) 52, 295
Bme1390I CCNGG 2 cut(s) 94, 416
Bme18I GGWCC 1 cut(s) 354
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 1 cut(s) 354
BmiI GGNNCC 4 cut(s) 6, 245, 334, 355
BmrFI CCNGG 2 cut(s) 94, 416
BpmI CTGGAG 3 cut(s) 93, 179, 282
Bpu10I CCTNAGC 1 cut(s) 523
Bpu1102I GCTNAGC 1 cut(s) 563
BpuEI CTTGAG 2 cut(s) 113, 578
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 486
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 205, 299
BseAI TCCGGA 1 cut(s) 578
BseBI CCWGG 2 cut(s) 94, 416
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 2 cut(s) 386, 575
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMII CTCAG 2 cut(s) 537, 577
BseNI ACTGG 2 cut(s) 205, 299
BseRI GAGGAG 3 cut(s) 44, 417, 420
BseSI GKGCMC 3 cut(s) 164, 306, 450
BseXI GCAGC 2 cut(s) 62, 280
BsiHKAI GWGCWC 2 cut(s) 450, 564
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 579
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 5 cut(s) 164, 262, 306, 450, 564
Bsp13I TCCGGA 1 cut(s) 578
Bsp143I GATC 4 cut(s) 133, 186, 332, 409
Bsp1720I GCTNAGC 1 cut(s) 563
BspACI CCGC 1 cut(s) 528
BspCNI CTCAG 2 cut(s) 536, 576
BspEI TCCGGA 1 cut(s) 578
BspLI GGNNCC 4 cut(s) 6, 245, 334, 355
BspPI GGATC 3 cut(s) 141, 327, 340
BsrI ACTGG 2 cut(s) 205, 299
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 4 cut(s) 133, 186, 332, 409
BssNI GRCGYC 1 cut(s) 459
BssSI CACGAG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 182
Bst2UI CCWGG 2 cut(s) 94, 416
Bst4CI ACNGT 1 cut(s) 539
Bst6I CTCTTC 1 cut(s) 435
BstACI GRCGYC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 523, 563
BstEII GGTNACC 2 cut(s) 467, 530
BstF5I GGATG 2 cut(s) 386, 575
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 1 cut(s) 500
BstKTI GATC 4 cut(s) 136, 189, 335, 412
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 4 cut(s) 133, 186, 332, 409
BstNI CCWGG 2 cut(s) 94, 416
BstNSI RCATGY 1 cut(s) 218
BstPI GGTNACC 2 cut(s) 467, 530
BstSCI CCNGG 2 cut(s) 92, 414
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 3 cut(s) 164, 306, 450
BstV1I GCAGC 2 cut(s) 62, 280
BstX2I RGATCY 3 cut(s) 133, 186, 332
BstXI CCANNNNNNTGG 1 cut(s) 53
BstYI RGATCY 3 cut(s) 133, 186, 332
BtsCI GGATG 2 cut(s) 386, 575
BtsIMutI CAGTG 2 cut(s) 306, 544
CfoI GCGC 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 354
CsiI ACCWGGT 1 cut(s) 92
Csp6I GTAC 1 cut(s) 90
CviAII CATG 3 cut(s) 70, 215, 307
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 2 cut(s) 523, 563
DpnI GATC 4 cut(s) 135, 188, 334, 411
DpnII GATC 4 cut(s) 133, 186, 332, 409
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Ecl136II GAGCTC 1 cut(s) 562
Eco24I GRGCYC 2 cut(s) 262, 564
Eco47I GGWCC 1 cut(s) 354
Eco47III AGCGCT 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 261
Eco91I GGTNACC 2 cut(s) 467, 530
EcoICRI GAGCTC 1 cut(s) 562
EcoO65I GGTNACC 2 cut(s) 467, 530
EcoRII CCWGG 2 cut(s) 92, 414
EcoT38I GRGCYC 2 cut(s) 262, 564
FaeI CATG 3 cut(s) 73, 218, 310
FaiI YATR 5 cut(s) 71, 144, 210, 216, 308
FatI CATG 3 cut(s) 69, 214, 306
Fnu4HI GCNGC 2 cut(s) 51, 294
FokI GGATG 2 cut(s) 373, 562
FriOI GRGCYC 2 cut(s) 262, 564
Fsp4HI GCNGC 2 cut(s) 51, 294
FspBI CTAG 2 cut(s) 315, 360
GlaI GCGC 1 cut(s) 499
GluI GCNGC 2 cut(s) 51, 294
GsuI CTGGAG 3 cut(s) 93, 179, 282
HaeII RGCGCY 1 cut(s) 501
HapII CCGG 1 cut(s) 579
HhaI GCGC 1 cut(s) 500
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 3 cut(s) 73, 218, 310
Hin6I GCGC 1 cut(s) 498
HinP1I GCGC 1 cut(s) 498
HinfI GANTC 3 cut(s) 100, 123, 178
HpaII CCGG 1 cut(s) 579
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 357, 448
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 6 cut(s) 110, 182, 247, 263, 557, 579
Hpy8I GTNNAC 2 cut(s) 357, 448
HpyAV CCTTC 2 cut(s) 469, 485
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4IV ACGT 3 cut(s) 252, 459, 549
HpyCH4V TGCA 2 cut(s) 394, 448
HpyF3I CTNAG 2 cut(s) 523, 563
HpySE526I ACGT 3 cut(s) 252, 459, 549
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 3 cut(s) 73, 218, 310
HspAI GCGC 1 cut(s) 498
Kpn2I TCCGGA 1 cut(s) 578
Kzo9I GATC 4 cut(s) 133, 186, 332, 409
LmnI GCTCC 2 cut(s) 301, 581
Lsp1109I GCAGC 2 cut(s) 62, 280
MabI ACCWGGT 1 cut(s) 92
MaeI CTAG 2 cut(s) 315, 360
MaeII ACGT 3 cut(s) 252, 459, 549
MaeIII GTNAC 3 cut(s) 467, 506, 530
MalI GATC 4 cut(s) 135, 188, 334, 411
MboI GATC 4 cut(s) 133, 186, 332, 409
MboII GAAGA 1 cut(s) 422
MflI RGATCY 3 cut(s) 133, 186, 332
MhlI GDGCHC 5 cut(s) 164, 262, 306, 450, 564
MluCI AATT 2 cut(s) 346, 589
MroI TCCGGA 1 cut(s) 578
MroXI GAANNNNTTC 1 cut(s) 316
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 2 cut(s) 53, 199
MspI CCGG 1 cut(s) 579
MspR9I CCNGG 2 cut(s) 94, 416
MvaI CCWGG 2 cut(s) 94, 416
NdeII GATC 4 cut(s) 133, 186, 332, 409
NlaIII CATG 3 cut(s) 73, 218, 310
NlaIV GGNNCC 4 cut(s) 6, 245, 334, 355
NspI RCATGY 1 cut(s) 218
PaeR7I CTCGAG 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PdmI GAANNNNTTC 1 cut(s) 316
PfeI GAWTC 3 cut(s) 100, 123, 178
PkrI GCNGC 2 cut(s) 52, 295
Psp124BI GAGCTC 1 cut(s) 564
Psp1406I AACGTT 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 92, 414
PspEI GGTNACC 2 cut(s) 467, 530
PspGI CCWGG 2 cut(s) 92, 414
PspN4I GGNNCC 4 cut(s) 6, 245, 334, 355
PspPI GGNCC 1 cut(s) 354
PsuI RGATCY 3 cut(s) 133, 186, 332
PvuII CAGCTG 2 cut(s) 53, 199
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
SacI GAGCTC 1 cut(s) 564
SaqAI TTAA 1 cut(s) 156
SatI GCNGC 2 cut(s) 51, 294
Sau3AI GATC 4 cut(s) 133, 186, 332, 409
Sau96I GGNCC 1 cut(s) 354
ScrFI CCNGG 2 cut(s) 94, 416
SduI GDGCHC 5 cut(s) 164, 262, 306, 450, 564
SexAI ACCWGGT 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 1 cut(s) 354
SlaI CTCGAG 1 cut(s) 261
SmlI CTYRAG 3 cut(s) 128, 261, 557
SmoI CTYRAG 3 cut(s) 128, 261, 557
SpeI ACTAGT 2 cut(s) 314, 359
Sse9I AATT 2 cut(s) 346, 589
SsiI CCGC 1 cut(s) 528
SspMI CTAG 2 cut(s) 315, 360
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 2 cut(s) 92, 414
TaaI ACNGT 1 cut(s) 539
TaiI ACGT 3 cut(s) 255, 462, 552
TaqI TCGA 1 cut(s) 262
TasI AATT 2 cut(s) 346, 589
TfiI GAWTC 3 cut(s) 100, 123, 178
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TscAI CASTG 2 cut(s) 306, 544
TseI GCWGC 2 cut(s) 50, 293
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 2 cut(s) 306, 544
VneI GTGCAC 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 354
XceI RCATGY 1 cut(s) 218
XhoI CTCGAG 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 316
XspI CTAG 2 cut(s) 315, 360
ZraI GACGTC 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.