FvH4_4g17370

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000519
Physical Location & Seq
Reverse (-)
21292148 .. 21292858
711 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17370.t1

Sequence Viewer

Length: 450 bp
ATGAAGGTGAAGCGTTTATATACCCTGGGGGAGGCAGAGCTCGGAGACTCACCTTCTTTCTCTTGGAGGCGTATAATTCAAGGCAGGCCAGTCCTTAAAGCTGGGATTCAATGGCAGGCAGACTTGGAATGTTTCCAAAATCCAAACCAACATAATGGAGGACTAGGTGGTGTCCTCATGCTCATAGATGAAGATGGGATGTTCAAGGCAGCCTTTGCTGTTCCAGTCACAAACGTAGCTACTGAGAAACAAGTGGAGCTTTTGGCCATAAAAGAAGGGTTTCAGCTTCTGCAAAAAATGCAGTTCGGTGGATTGCTTGATGATATAAAAGTGGCTTTAAACAATGTACCTTCTGTTAAGATAGGCCATGCTCCAAGATCTTGTAAAGTGGCACATAGGTTAGCTAGCTTGGCTTTTGAAGCCACAGAGATAGTCACTTCTTTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

150

Amino Acids

16.3

Weight (kDa)

7.8

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 264
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 1 cut(s) 31
AgsI TTSAA 4 cut(s) 80, 110, 205, 419
AjnI CCWGG 1 cut(s) 24
AluBI AGCT 7 cut(s) 40, 101, 239, 259, 286, 404, 408
AluI AGCT 7 cut(s) 40, 101, 239, 259, 286, 404, 408
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 1 cut(s) 39
AlwNI CAGNNNCTG 1 cut(s) 289
AoxI GGCC 3 cut(s) 86, 264, 364
ApeKI GCWGC 1 cut(s) 209
Asp700I GAANNNNTTC 1 cut(s) 279
AsuHPI GGTGA 2 cut(s) 19, 42
AsuNHI GCTAGC 1 cut(s) 404
BalI TGGCCA 1 cut(s) 266
BanII GRGCYC 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 1 cut(s) 221
BccI CCATC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 2 cut(s) 164, 405
BglII AGATCT 1 cut(s) 377
BisI GCNGC 1 cut(s) 210
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 26
BmrFI CCNGG 1 cut(s) 26
BmtI GCTAGC 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 24, 25
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse1I ACTGG 2 cut(s) 89, 224
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 2 cut(s) 24, 25
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 2 cut(s) 89, 224
BseXI GCAGC 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 101
BshFI GGCC 3 cut(s) 88, 266, 366
BsiHKAI GWGCWC 1 cut(s) 42
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 39
BsnI GGCC 3 cut(s) 88, 266, 366
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 1 cut(s) 377
BspANI GGCC 3 cut(s) 88, 266, 366
BspCNI CTCAG 1 cut(s) 235
BspOI GCTAGC 1 cut(s) 408
BsrI ACTGG 2 cut(s) 89, 224
BssECI CCNNGG 2 cut(s) 24, 25
BssMI GATC 1 cut(s) 377
Bst2UI CCWGG 1 cut(s) 26
BstAPI GCANNNNNTGC 2 cut(s) 215, 298
BstC8I GCNNGC 3 cut(s) 86, 117, 406
BstDEI CTNAG 1 cut(s) 243
BstENI CCTNNNNNAGG 1 cut(s) 29
BstF5I GGATG 1 cut(s) 204
BstKTI GATC 1 cut(s) 380
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 1 cut(s) 377
BstMWI GCNNNNNNNGC 4 cut(s) 215, 298, 410, 419
BstNI CCWGG 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 24
BstV1I GCAGC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 377
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 3 cut(s) 88, 266, 366
BtsCI GGATG 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 86, 117, 406
CaiI CAGNNNCTG 1 cut(s) 289
Csp6I GTAC 1 cut(s) 347
CviAII CATG 2 cut(s) 178, 368
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 1 cut(s) 243
DpnI GATC 1 cut(s) 379
DpnII GATC 1 cut(s) 377
DraI TTTAAA 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 42
Eco53kI GAGCTC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 40
EcoNI CCTNNNNNAGG 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 42
FaeI CATG 2 cut(s) 181, 371
FalI AAGNNNNNCTT 4 cut(s) 197, 229, 243, 275
FatI CATG 2 cut(s) 177, 367
Fnu4HI GCNGC 1 cut(s) 210
FokI GGATG 1 cut(s) 211
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 210
FspBI CTAG 2 cut(s) 164, 405
GluI GCNGC 1 cut(s) 210
GsaI CCCAGC 1 cut(s) 105
HaeIII GGCC 3 cut(s) 88, 266, 366
Hin1II CATG 2 cut(s) 181, 371
HinfI GANTC 2 cut(s) 47, 106
HphI GGTGA 2 cut(s) 19, 42
Hpy188I TCNGA 1 cut(s) 44
HpyAV CCTTC 3 cut(s) 63, 269, 360
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 2 cut(s) 292, 301
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 298, 410, 419
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 2 cut(s) 181, 371
Kzo9I GATC 1 cut(s) 377
LmnI GCTCC 2 cut(s) 256, 376
LpnPI CCDG 7 cut(s) 11, 38, 70, 87, 101, 102, 237
Lsp1109I GCAGC 1 cut(s) 221
MaeI CTAG 2 cut(s) 164, 405
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 226, 433
MalI GATC 1 cut(s) 379
MboI GATC 1 cut(s) 377
MboII GAAGA 1 cut(s) 203
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 4 cut(s) 25, 60, 152, 185
Mox20I TGGCCA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 266
MseI TTAA 3 cut(s) 96, 338, 357
Msp20I TGGCCA 1 cut(s) 266
MspR9I CCNGG 1 cut(s) 26
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 4 cut(s) 215, 298, 410, 419
NdeII GATC 1 cut(s) 377
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 2 cut(s) 181, 371
NmuCI GTSAC 2 cut(s) 226, 433
PasI CCCWGGG 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 106
PkrI GCNGC 1 cut(s) 211
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 101
PspGI CCWGG 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 289
PsuI RGATCY 1 cut(s) 377
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 3 cut(s) 96, 338, 357
SatI GCNGC 1 cut(s) 210
Sau3AI GATC 1 cut(s) 377
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 26
SduI GDGCHC 1 cut(s) 42
Sse9I AATT 1 cut(s) 75
SspMI CTAG 2 cut(s) 164, 405
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 24
TaiI ACGT 1 cut(s) 237
TasI AATT 1 cut(s) 75
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 3 cut(s) 96, 338, 357
Tru9I TTAA 3 cut(s) 96, 338, 357
TseFI GTSAC 2 cut(s) 226, 433
TseI GCWGC 1 cut(s) 209
Tsp45I GTSAC 2 cut(s) 226, 433
TspDTI ATGAA 2 cut(s) 17, 204
XagI CCTNNNNNAGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 2 cut(s) 164, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.