Rh4BG212500

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000519
Physical Location & Seq
Reverse (-)
36977631 .. 36985177
7547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG212500.1

Sequence Viewer

Length: 486 bp
ATGTTGGACAAACGACATTCAAAAAGATCAGATCTACTCATCTTTTTGCCTCAAATAACGCACGGCGGAACCAGGGGAATACTAAGAGATGATACAGGAAACTTTCGAGTTGCTTTTGCAGTCCCTGTACAGTCAGTTGTCTCGGCCAAACATGTAGAGTTATGTGTCATTGATGAGGGAATTCAGCTGTTGAAGCAGTTGCAGATCAGCAGCGCTGTCATTGAGACAGACTGCTTGGAAGCTATTCAAGACATTGCTGAAACACACTTTACTCATGTAGCTACCGAAGACCTTCTTGACAATATCAGAGGTGAGCTACTTTCCAGGCCAAACATCATATTGCAGCATGCTCCTCGGAGTTGTAACAGAGTAGCACATTGCTTAGCTAATTATGCCTATGAGGCTAGAGCTAGCTCCATTTGGCTTGATCATCGTCCTGCCCTTATTATAAACCTTATCAAGTTTGATTGTAACCATATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

161

Amino Acids

18.04

Weight (kDa)

6.28

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 19 - 132 1.7e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AciI CCGC 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 180
AfaI GTAC 1 cut(s) 129
AfeI AGCGCT 1 cut(s) 214
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 3 cut(s) 21, 193, 248
AjnI CCWGG 2 cut(s) 71, 323
AluBI AGCT 7 cut(s) 187, 242, 281, 316, 386, 410, 414
AluI AGCT 7 cut(s) 187, 242, 281, 316, 386, 410, 414
Alw26I GTCTC 2 cut(s) 145, 218
AlwNI CAGNNNCTG 1 cut(s) 125
Aor51HI AGCGCT 1 cut(s) 214
AoxI GGCC 2 cut(s) 144, 326
ApeKI GCWGC 2 cut(s) 210, 343
ApoI RAATTY 1 cut(s) 180
Asp700I GAANNNNTTC 2 cut(s) 243, 291
AspLEI GCGC 1 cut(s) 215
AsuHPI GGTGA 1 cut(s) 323
AsuNHI GCTAGC 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 294
BbvI GCAGC 2 cut(s) 222, 355
BceAI ACGGC 1 cut(s) 79
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 2 cut(s) 73, 325
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 2 cut(s) 145, 218
BfaI CTAG 2 cut(s) 405, 411
BfoI RGCGCY 1 cut(s) 216
BglI GCCNNNNNGGC 1 cut(s) 401
BglII AGATCT 1 cut(s) 31
BisI GCNGC 2 cut(s) 211, 344
BlpI GCTNAGC 1 cut(s) 382
BlsI GCNGC 2 cut(s) 212, 345
Bme1390I CCNGG 2 cut(s) 73, 325
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 2 cut(s) 73, 325
BmtI GCTAGC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 294
Bpu1102I GCTNAGC 1 cut(s) 382
BsaJI CCNNGG 2 cut(s) 72, 353
Bse3DI GCAATG 2 cut(s) 252, 376
BseBI CCWGG 2 cut(s) 73, 325
BseDI CCNNGG 2 cut(s) 72, 353
BseMI GCAATG 2 cut(s) 252, 376
BseRI GAGGAG 1 cut(s) 342
BseXI GCAGC 2 cut(s) 222, 355
BshFI GGCC 2 cut(s) 146, 328
BslFI GGGAC 1 cut(s) 107
BsmAI GTCTC 2 cut(s) 145, 218
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 2 cut(s) 146, 328
Bsp1407I TGTACA 1 cut(s) 127
Bsp143I GATC 4 cut(s) 26, 31, 204, 427
Bsp1720I GCTNAGC 1 cut(s) 382
BspACI CCGC 1 cut(s) 66
BspANI GGCC 2 cut(s) 146, 328
BspLI GGNNCC 1 cut(s) 70
BspOI GCTAGC 1 cut(s) 414
BsrDI GCAATG 2 cut(s) 252, 376
BsrGI TGTACA 1 cut(s) 127
BssECI CCNNGG 2 cut(s) 72, 353
BssMI GATC 4 cut(s) 26, 31, 204, 427
Bst2UI CCWGG 2 cut(s) 73, 325
Bst4CI ACNGT 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 127
BstC8I GCNNGC 2 cut(s) 348, 412
BstDEI CTNAG 2 cut(s) 83, 382
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 1 cut(s) 215
BstKTI GATC 4 cut(s) 29, 34, 207, 430
BstMAI GTCTC 2 cut(s) 145, 218
BstMBI GATC 4 cut(s) 26, 31, 204, 427
BstMWI GCNNNNNNNGC 3 cut(s) 193, 392, 401
BstNI CCWGG 2 cut(s) 73, 325
BstNSI RCATGY 2 cut(s) 155, 350
BstSCI CCNGG 2 cut(s) 71, 323
BstV1I GCAGC 2 cut(s) 222, 355
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 2 cut(s) 146, 328
Cac8I GCNNGC 2 cut(s) 348, 412
CaiI CAGNNNCTG 1 cut(s) 125
CfoI GCGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 128
CviAII CATG 3 cut(s) 152, 275, 347
CviQI GTAC 1 cut(s) 128
DdeI CTNAG 2 cut(s) 83, 382
DpnI GATC 4 cut(s) 28, 33, 206, 429
DpnII GATC 4 cut(s) 26, 31, 204, 427
EaeI YGGCCR 1 cut(s) 144
EciI GGCGGA 1 cut(s) 81
Eco47III AGCGCT 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 180
EcoRII CCWGG 2 cut(s) 71, 323
FaeI CATG 3 cut(s) 155, 278, 350
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 107
FatI CATG 3 cut(s) 151, 274, 346
FauNDI CATATG 1 cut(s) 477
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 2 cut(s) 211, 344
Fsp4HI GCNGC 2 cut(s) 211, 344
FspBI CTAG 2 cut(s) 405, 411
GlaI GCGC 1 cut(s) 214
GluI GCNGC 2 cut(s) 211, 344
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 2 cut(s) 146, 328
HhaI GCGC 1 cut(s) 215
Hin1II CATG 3 cut(s) 155, 278, 350
Hin6I GCGC 1 cut(s) 213
HinP1I GCGC 1 cut(s) 213
HphI GGTGA 1 cut(s) 323
Hpy188I TCNGA 3 cut(s) 31, 308, 357
Hpy188III TCNNGA 2 cut(s) 248, 296
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 1 cut(s) 132
HpyCH4V TGCA 3 cut(s) 119, 202, 343
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 392, 401
HpyF3I CTNAG 2 cut(s) 83, 382
Hsp92II CATG 3 cut(s) 155, 278, 350
HspAI GCGC 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 4 cut(s) 26, 31, 204, 427
LmnI GCTCC 2 cut(s) 355, 419
LpnPI CCDG 7 cut(s) 58, 81, 85, 138, 310, 337, 450
Lsp1109I GCAGC 2 cut(s) 222, 355
MaeI CTAG 2 cut(s) 405, 411
MaeIII GTNAC 2 cut(s) 362, 470
MalI GATC 4 cut(s) 28, 33, 206, 429
MboI GATC 4 cut(s) 26, 31, 204, 427
MboII GAAGA 1 cut(s) 299
MflI RGATCY 1 cut(s) 31
MluCI AATT 2 cut(s) 180, 388
MnlI CCTC 5 cut(s) 60, 169, 302, 363, 394
MroXI GAANNNNTTC 2 cut(s) 243, 291
MspA1I CMGCKG 1 cut(s) 187
MspR9I CCNGG 2 cut(s) 73, 325
MvaI CCWGG 2 cut(s) 73, 325
MwoI GCNNNNNNNGC 3 cut(s) 193, 392, 401
NdeI CATATG 1 cut(s) 477
NdeII GATC 4 cut(s) 26, 31, 204, 427
NheI GCTAGC 1 cut(s) 410
NlaIII CATG 3 cut(s) 155, 278, 350
NlaIV GGNNCC 1 cut(s) 70
NmeAIII GCCGAG 1 cut(s) 122
NspI RCATGY 2 cut(s) 155, 350
PaeI GCATGC 1 cut(s) 350
PciI ACATGT 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 243, 291
PkrI GCNGC 2 cut(s) 212, 345
PscI ACATGT 1 cut(s) 151
PsiI TTATAA 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 71, 323
PspGI CCWGG 2 cut(s) 71, 323
PspN4I GGNNCC 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 1 cut(s) 129
RsaNI GTAC 1 cut(s) 128
SatI GCNGC 2 cut(s) 211, 344
Sau3AI GATC 4 cut(s) 26, 31, 204, 427
ScrFI CCNGG 2 cut(s) 73, 325
SphI GCATGC 1 cut(s) 350
Sse9I AATT 2 cut(s) 180, 388
SsiI CCGC 1 cut(s) 66
SspMI CTAG 2 cut(s) 405, 411
StyD4I CCNGG 2 cut(s) 71, 323
TaaI ACNGT 1 cut(s) 132
TaqI TCGA 1 cut(s) 106
TasI AATT 2 cut(s) 180, 388
TatI WGTACW 1 cut(s) 127
TseI GCWGC 2 cut(s) 210, 343
XapI RAATTY 1 cut(s) 180
XceI RCATGY 2 cut(s) 155, 350
XmnI GAANNNNTTC 2 cut(s) 243, 291
XspI CTAG 2 cut(s) 405, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.