Rmu_sc0001654.1_g000024

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001654.1
OG0000519
Physical Location & Seq
Forward (+)
82189 .. 83279
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001654.1_g000024.1.cds

Sequence Viewer

Length: 1002 bp
atgatagattgtgttggagtccagaaaagagaggtcgatcgttttacaatgaagacagcttattggattgcccgtgaatctgtacttggaaatgttctgaccagtacttcgaatggagatccttttaatatgttatggaagactatatggaaagcaaaggttcttggtaaagtgcagatatgcatttggcgagctacaaagaatctcttgccaacaagagcatgtctaacaacaaagggatacacaggtgatagcagctgcgtcctctgtaatcacaggttagaagatgtatctcatgtattgtgcaaatgtcctattgcagttgagctcctatcttccactcctcttaatctccaacaatccttactacctcatataaactccaaagaatggctactggattgtgctatgcatctgaatcttgctacctttgaaaggttgatgatggtcttatggtctgtgtggaagaatagaaacagtaagctctgggatggtgtggctcaaacaacacctaatattcttctatactccatggcctggttggaagagttccacaaagctcgtcatgtgcatcacaacatgggagatagaagtattaagtcgccttggaagccgacaactgagggtcgaattaagttgaatgtggatggatctttcattccaggtatgcaaagaggaggggcaggtggtgtcattcgaggggaacaaggtctctttattgcagcttttgcccatactatgccccatgtagcctcggcaaagaacagtacaactaatgaggaccacgactttgatgagcaaagagctgtcttgtatgacattgcagtccttgtgaatgcacttccgggtgtagagattaagcatgcctttcgtacttgtaacaatgtggtacatagattagctgcaattgcttttaagtcaaaccaacgtgaggtctggttgcacgttccgccaaactctactactgatgtactccagcatgacctagctcatgcgacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

333

Amino Acids

37.46

Weight (kDa)

8.94

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 674
AasI GACNNNNNNGTC 1 cut(s) 824
Acc36I ACCTGC 1 cut(s) 674
AccB7I CCANNNNNTGG 2 cut(s) 390, 537
AciI CCGC 1 cut(s) 950
AclWI GGATC 2 cut(s) 113, 658
AfaI GTAC 6 cut(s) 84, 106, 769, 874, 891, 972
AfiI CCNNNNNNNGG 4 cut(s) 390, 435, 537, 931
AgsI TTSAA 2 cut(s) 434, 640
AjnI CCWGG 2 cut(s) 536, 661
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 716
AlwI GGATC 2 cut(s) 113, 658
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 4 cut(s) 255, 258, 722, 902
ArsI GACNNNNNNTTYG 4 cut(s) 91, 123, 773, 805
AspS9I GGNCC 1 cut(s) 781
AsuC2I CCSGG 1 cut(s) 846
AsuHPI GGTGA 1 cut(s) 260
AsuII TTCGAA 1 cut(s) 110
AvaII GGWCC 1 cut(s) 781
BaeI ACNNNNGTAYC 2 cut(s) 232, 265
BanII GRGCYC 1 cut(s) 330
BbsI GAAGAC 2 cut(s) 59, 146
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 4 cut(s) 245, 267, 734, 889
BccI CCATC 3 cut(s) 439, 485, 641
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 2 cut(s) 538, 663
BciVI GTATCC 1 cut(s) 233
BcnI CCSGG 1 cut(s) 846
BcoDI GTCTC 1 cut(s) 716
BfaI CTAG 1 cut(s) 986
BfuAI ACCTGC 1 cut(s) 674
BfuI GTATCC 1 cut(s) 233
BisI GCNGC 4 cut(s) 256, 259, 723, 903
BlsI GCNGC 4 cut(s) 257, 260, 724, 904
BmcAI AGTACT 1 cut(s) 106
Bme1390I CCNGG 3 cut(s) 538, 663, 846
Bme18I GGWCC 1 cut(s) 781
BmgT120I GGNCC 1 cut(s) 781
BmrFI CCNGG 3 cut(s) 538, 663, 846
BmsI GCATC 2 cut(s) 421, 580
BpiI GAAGAC 2 cut(s) 59, 146
BpmI CTGGAG 1 cut(s) 959
Bpu14I TTCGAA 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 846
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 3 cut(s) 531, 605, 753
Bsc4I CCNNNNNNNGG 4 cut(s) 390, 435, 537, 931
Bse1I ACTGG 2 cut(s) 102, 402
Bse3DI GCAATG 1 cut(s) 819
BseBI CCWGG 2 cut(s) 538, 663
BseDI CCNNGG 3 cut(s) 531, 605, 753
BseGI GGATG 2 cut(s) 496, 652
BseLI CCNNNNNNNGG 4 cut(s) 390, 435, 537, 931
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 1 cut(s) 612
BseNI ACTGG 2 cut(s) 102, 402
BseRI GAGGAG 2 cut(s) 333, 690
BseXI GCAGC 4 cut(s) 245, 267, 734, 889
BsgI GTGCAG 1 cut(s) 194
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 1 cut(s) 536
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 1 cut(s) 845
BslI CCNNNNNNNGG 4 cut(s) 390, 435, 537, 931
BsmAI GTCTC 1 cut(s) 716
BsmI GAATGC 1 cut(s) 841
BsnI GGCC 1 cut(s) 536
Bso31I GGTCTC 1 cut(s) 716
Bsp119I TTCGAA 1 cut(s) 110
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 3 cut(s) 37, 118, 650
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 1 cut(s) 950
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 1 cut(s) 613
BspMI ACCTGC 1 cut(s) 674
BspPI GGATC 2 cut(s) 113, 658
BspT104I TTCGAA 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 716
BsrDI GCAATG 1 cut(s) 819
BsrI ACTGG 2 cut(s) 102, 402
BssECI CCNNGG 3 cut(s) 531, 605, 753
BssMI GATC 3 cut(s) 37, 118, 650
BssT1I CCWWGG 2 cut(s) 531, 605
Bst2UI CCWGG 2 cut(s) 538, 663
Bst4CI ACNGT 2 cut(s) 479, 767
Bst6I CTCTTC 1 cut(s) 540
BstAPI GCANNNNNTGC 1 cut(s) 728
BstBI TTCGAA 1 cut(s) 110
BstC8I GCNNGC 2 cut(s) 192, 864
BstDEI CTNAG 1 cut(s) 621
BstDSI CCRYGG 1 cut(s) 531
BstENI CCTNNNNNAGG 1 cut(s) 433
BstF5I GGATG 2 cut(s) 496, 652
BstKTI GATC 3 cut(s) 40, 121, 653
BstMAI GTCTC 1 cut(s) 716
BstMBI GATC 3 cut(s) 37, 118, 650
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 4 cut(s) 610, 728, 908, 949
BstNI CCWGG 2 cut(s) 538, 663
BstNSI RCATGY 2 cut(s) 225, 866
BstSCI CCNGG 3 cut(s) 536, 661, 844
BstV1I GCAGC 4 cut(s) 245, 267, 734, 889
BstV2I GAAGAC 2 cut(s) 59, 146
BstX2I RGATCY 2 cut(s) 118, 650
BstYI RGATCY 2 cut(s) 118, 650
BsuI GTATCC 1 cut(s) 233
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 2 cut(s) 496, 652
BveI ACCTGC 1 cut(s) 674
Cac8I GCNNGC 2 cut(s) 192, 864
Cfr13I GGNCC 1 cut(s) 781
CseI GACGC 1 cut(s) 250
Csp6I GTAC 6 cut(s) 83, 105, 768, 873, 890, 971
CviAII CATG 9 cut(s) 222, 296, 532, 566, 580, 746, 863, 980, 992
CviQI GTAC 6 cut(s) 83, 105, 768, 873, 890, 971
DdeI CTNAG 1 cut(s) 621
DpnI GATC 3 cut(s) 39, 120, 652
DpnII GATC 3 cut(s) 37, 118, 650
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
EciI GGCGGA 1 cut(s) 939
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 531, 605
Eco24I GRGCYC 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 716
Eco47I GGWCC 1 cut(s) 781
Eco53kI GAGCTC 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 433
EcoRII CCWGG 2 cut(s) 536, 661
EcoT14I CCWWGG 2 cut(s) 531, 605
EcoT22I ATGCAT 2 cut(s) 185, 414
EcoT38I GRGCYC 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 531, 605
FaeI CATG 9 cut(s) 225, 299, 535, 569, 583, 749, 866, 983, 995
FalI AAGNNNNNCTT 4 cut(s) 191, 223, 851, 883
FatI CATG 9 cut(s) 221, 295, 531, 565, 579, 745, 862, 979, 991
Fnu4HI GCNGC 4 cut(s) 256, 259, 723, 903
FokI GGATG 2 cut(s) 503, 659
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 4 cut(s) 256, 259, 723, 903
FspBI CTAG 1 cut(s) 986
GluI GCNGC 4 cut(s) 256, 259, 723, 903
GsuI CTGGAG 1 cut(s) 959
HaeIII GGCC 1 cut(s) 536
HapII CCGG 1 cut(s) 845
HgaI GACGC 1 cut(s) 250
Hin1II CATG 9 cut(s) 225, 299, 535, 569, 583, 749, 866, 983, 995
HinfI GANTC 4 cut(s) 18, 77, 202, 418
HpaII CCGG 1 cut(s) 845
HphI GGTGA 1 cut(s) 260
Hpy188I TCNGA 2 cut(s) 99, 417
Hpy188III TCNNGA 1 cut(s) 22
HpyCH4III ACNGT 2 cut(s) 479, 767
HpyCH4IV ACGT 2 cut(s) 928, 945
HpyF10VI GCNNNNNNNGC 4 cut(s) 610, 728, 908, 949
HpyF3I CTNAG 1 cut(s) 621
HpySE526I ACGT 2 cut(s) 928, 945
Hsp92II CATG 9 cut(s) 225, 299, 535, 569, 583, 749, 866, 983, 995
Kzo9I GATC 3 cut(s) 37, 118, 650
LmnI GCTCC 1 cut(s) 333
Lsp1109I GCAGC 4 cut(s) 245, 267, 734, 889
LweI GCATC 2 cut(s) 421, 580
MaeI CTAG 1 cut(s) 986
MaeII ACGT 2 cut(s) 928, 945
MaeIII GTNAC 1 cut(s) 878
MalI GATC 3 cut(s) 39, 120, 652
MboI GATC 3 cut(s) 37, 118, 650
MboII GAAGA 7 cut(s) 64, 151, 296, 327, 478, 512, 557
MfeI CAATTG 1 cut(s) 906
MflI RGATCY 2 cut(s) 118, 650
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 2 cut(s) 630, 906
MlyI GAGTC 1 cut(s) 27
MmeI TCCRAC 2 cut(s) 379, 522
Mph1103I ATGCAT 2 cut(s) 185, 414
MseI TTAA 7 cut(s) 126, 348, 597, 633, 858, 915, 1000
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 1 cut(s) 845
MspR9I CCNGG 3 cut(s) 538, 663, 846
MunI CAATTG 1 cut(s) 906
Mva1269I GAATGC 1 cut(s) 841
MvaI CCWGG 2 cut(s) 538, 663
MwoI GCNNNNNNNGC 4 cut(s) 610, 728, 908, 949
NciI CCSGG 1 cut(s) 846
NcoI CCATGG 1 cut(s) 531
NdeII GATC 3 cut(s) 37, 118, 650
NlaIII CATG 9 cut(s) 225, 299, 535, 569, 583, 749, 866, 983, 995
NmeAIII GCCGAG 1 cut(s) 734
NsiI ATGCAT 2 cut(s) 185, 414
NspI RCATGY 2 cut(s) 225, 866
NspV TTCGAA 1 cut(s) 110
PaeI GCATGC 1 cut(s) 866
PaqCI CACCTGC 1 cut(s) 674
PctI GAATGC 1 cut(s) 841
PfeI GAWTC 3 cut(s) 77, 202, 418
PflMI CCANNNNNTGG 2 cut(s) 390, 537
PkrI GCNGC 4 cut(s) 257, 260, 724, 904
Ple19I CGATCG 1 cut(s) 40
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 536, 661
PspGI CCWGG 2 cut(s) 536, 661
PspPI GGNCC 1 cut(s) 781
PsuI RGATCY 2 cut(s) 118, 650
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 258
RsaI GTAC 6 cut(s) 84, 106, 769, 874, 891, 972
RsaNI GTAC 6 cut(s) 83, 105, 768, 873, 890, 971
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 7 cut(s) 126, 348, 597, 633, 858, 915, 1000
SatI GCNGC 4 cut(s) 256, 259, 723, 903
Sau3AI GATC 3 cut(s) 37, 118, 650
Sau96I GGNCC 1 cut(s) 781
ScaI AGTACT 1 cut(s) 106
SchI GAGTC 1 cut(s) 27
ScrFI CCNGG 3 cut(s) 538, 663, 846
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 2 cut(s) 421, 580
SfuI TTCGAA 1 cut(s) 110
SinI GGWCC 1 cut(s) 781
SphI GCATGC 1 cut(s) 866
Sse9I AATT 2 cut(s) 630, 906
SsiI CCGC 1 cut(s) 950
SspI AATATT 1 cut(s) 517
SspMI CTAG 1 cut(s) 986
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 3 cut(s) 536, 661, 844
StyI CCWWGG 2 cut(s) 531, 605
TaaI ACNGT 2 cut(s) 479, 767
TaiI ACGT 2 cut(s) 931, 948
TaqI TCGA 4 cut(s) 36, 110, 628, 697
TasI AATT 2 cut(s) 630, 906
TatI WGTACW 4 cut(s) 82, 104, 767, 970
TfiI GAWTC 3 cut(s) 77, 202, 418
Tru1I TTAA 7 cut(s) 126, 348, 597, 633, 858, 915, 1000
Tru9I TTAA 7 cut(s) 126, 348, 597, 633, 858, 915, 1000
TseI GCWGC 4 cut(s) 255, 258, 722, 902
TspDTI ATGAA 2 cut(s) 65, 646
Van91I CCANNNNNTGG 2 cut(s) 390, 537
VpaK11BI GGWCC 1 cut(s) 781
XagI CCTNNNNNAGG 1 cut(s) 433
XceI RCATGY 2 cut(s) 225, 866
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XspI CTAG 1 cut(s) 986
ZrmI AGTACT 1 cut(s) 106
Zsp2I ATGCAT 2 cut(s) 185, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.