Rorug06G0072000

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000519
Physical Location & Seq
Forward (+)
9581980 .. 9592073
10094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0072000.1

Sequence Viewer

Length: 813 bp
ATGAATCCAATCCTTCTCCTTCTCCTCTTCTCCCTTCAGGCCACCCTCTCCCACGCCACCACAGCCTACCCTTCCATTCCGGGCACCACCCCCGTCGACGTCTCCTCCGGCGCCACCGCCTCCAACCTGATCCCCCCTCGCCGCGAAGTCCACGGAAACGGCCGAATCTTCGACATCACCCACCGCTACAGCCCCGACATGCCGGCGTTTGGCTCCGCCGACGGCGTCGGCCAGTTCCTCTGGCTCCCCCACAGCATGAAGAACGGCTCCATTGCTAACAACTCCGAGATGAAGCTCCCCACCCACACCGGGACCCACGTCGACGCCCCCGGCCACTTCTTCGATCACTACTTCGACGCCGGCTTCGACGTCGACACGCTCGACTTGGATGTCCTTAATGGTGCGGCATTGTTAGTTGACGTTCCGAGGGATAAGAACATTACTGCTGAAGTCATGGAGGGCTTACATATTCCCAAGGGCGTGCGTCGAGTACTTTTCAGAACTTTGAACACGGATAGACGGCTTATGTTCCAAAAGGAGTTTGACAGCAGCTATGTGGGATTTATGAAGGATGGAGCTAAGTGGTTGGTGGAGAACACTGACATCAAACTGATTGGAATTGATTACTTATCTGCTGCTGCATATGATGATTTGATTCCATCTCACCTTGTTTTGCTAGAAGGCAGGGAGATCATTCTTGTAGAAGGTCTAAAACTTGATGACATCCAACCAGGAATATATTCTGTCCATTGCTTACCTTTGAGGCTAACAGGTGCAGAAGGATCACCAATCAGATGCATTCTTATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

270

Amino Acids

29.69

Weight (kDa)

5.6

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 57 - 212 7.6e-22 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 2 cut(s) 102, 372
AccB1I GGYRCC 2 cut(s) 83, 110
AccI GTMKAC 3 cut(s) 96, 321, 372
AccII CGCG 1 cut(s) 144
AciI CCGC 5 cut(s) 117, 142, 184, 216, 404
AclWI GGATC 2 cut(s) 124, 790
AcoI YGGCCR 3 cut(s) 160, 229, 331
AcuI CTGAAG 2 cut(s) 20, 468
AcyI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 2 cut(s) 209, 309
AgsI TTSAA 1 cut(s) 508
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 1 cut(s) 730
AluBI AGCT 3 cut(s) 295, 552, 578
AluI AGCT 3 cut(s) 295, 552, 578
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 2 cut(s) 124, 790
AoxI GGCC 4 cut(s) 39, 160, 229, 331
ApeKI GCWGC 3 cut(s) 549, 635, 638
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
Asp700I GAANNNNTTC 1 cut(s) 739
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 312
AsuC2I CCSGG 3 cut(s) 81, 310, 330
AsuHPI GGTGA 3 cut(s) 169, 656, 777
AvaII GGWCC 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 86
BanI GGYRCC 2 cut(s) 83, 110
BbvI GCAGC 3 cut(s) 561, 622, 625
BccI CCATC 2 cut(s) 566, 667
BceAI ACGGC 4 cut(s) 175, 238, 280, 536
BciT130I CCWGG 1 cut(s) 732
BcnI CCSGG 3 cut(s) 81, 310, 330
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 1 cut(s) 187
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 5 cut(s) 142, 405, 550, 636, 639
BlsI GCNGC 5 cut(s) 143, 406, 551, 637, 640
BmcAI AGTACT 1 cut(s) 492
Bme1390I CCNGG 4 cut(s) 81, 310, 330, 732
Bme18I GGWCC 1 cut(s) 312
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
BmrFI CCNGG 4 cut(s) 81, 310, 330, 732
BmsI GCATC 1 cut(s) 785
BoxI GACNNNNGTC 1 cut(s) 317
BpuMI CCSGG 3 cut(s) 81, 310, 330
BsaHI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BsaJI CCNNGG 4 cut(s) 151, 328, 425, 474
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 309
Bse118I RCCGGY 2 cut(s) 202, 359
Bse1I ACTGG 1 cut(s) 232
Bse3DI GCAATG 2 cut(s) 270, 748
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 4 cut(s) 151, 328, 425, 474
BseGI GGATG 3 cut(s) 394, 577, 723
BseLI CCNNNNNNNGG 2 cut(s) 209, 309
BseMI GCAATG 2 cut(s) 270, 748
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 14, 94
BseSI GKGCMC 1 cut(s) 86
BseX3I CGGCCG 1 cut(s) 160
BseXI GCAGC 3 cut(s) 561, 622, 625
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 163
BshFI GGCC 4 cut(s) 41, 162, 231, 333
BshNI GGYRCC 2 cut(s) 83, 110
BsiEI CGRYCG 1 cut(s) 163
BsiSI CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 2 cut(s) 209, 309
BsmAI GTCTC 1 cut(s) 106
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 798
BsnI GGCC 4 cut(s) 41, 162, 231, 333
Bsp1286I GDGCHC 1 cut(s) 86
Bsp143I GATC 4 cut(s) 129, 343, 690, 782
BspACI CCGC 5 cut(s) 117, 142, 184, 216, 404
BspANI GGCC 4 cut(s) 41, 162, 231, 333
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
BspPI GGATC 2 cut(s) 124, 790
BspT107I GGYRCC 2 cut(s) 83, 110
BsrDI GCAATG 2 cut(s) 270, 748
BsrFI RCCGGY 2 cut(s) 202, 359
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 2 cut(s) 202, 359
BssECI CCNNGG 4 cut(s) 151, 328, 425, 474
BssMI GATC 4 cut(s) 129, 343, 690, 782
BssNI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 732
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
BstC8I GCNNGC 3 cut(s) 204, 361, 482
BstDEI CTNAG 1 cut(s) 579
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 3 cut(s) 394, 577, 723
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 4 cut(s) 132, 346, 693, 785
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 4 cut(s) 129, 343, 690, 782
BstMCI CGRYCG 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 732
BstNSI RCATGY 1 cut(s) 202
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 4 cut(s) 79, 308, 328, 730
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 1 cut(s) 86
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 561, 622, 625
BstZI CGGCCG 1 cut(s) 160
BsuRI GGCC 4 cut(s) 41, 162, 231, 333
BtgI CCRYGG 1 cut(s) 151
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 3 cut(s) 394, 577, 723
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 3 cut(s) 204, 361, 482
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 2 cut(s) 202, 359
Cfr13I GGNCC 1 cut(s) 312
CseI GACGC 4 cut(s) 214, 332, 365, 473
Csp6I GTAC 1 cut(s) 491
CviAII CATG 3 cut(s) 199, 256, 454
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 1 cut(s) 579
DinI GGCGCC 1 cut(s) 112
DpnI GATC 4 cut(s) 131, 345, 692, 784
DpnII GATC 4 cut(s) 129, 343, 690, 782
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
EaeI YGGCCR 3 cut(s) 160, 229, 331
EagI CGGCCG 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 205
EclXI CGGCCG 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 474
Eco47I GGWCC 1 cut(s) 312
Eco52I CGGCCG 1 cut(s) 160
Eco57I CTGAAG 2 cut(s) 20, 468
EcoO109I RGGNCCY 1 cut(s) 312
EcoRII CCWGG 1 cut(s) 730
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 800
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 474
Esp3I CGTCTC 1 cut(s) 106
FaeI CATG 3 cut(s) 202, 259, 457
FaqI GGGAC 1 cut(s) 325
FatI CATG 3 cut(s) 198, 255, 453
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 3 cut(s) 96, 321, 372
Fnu4HI GCNGC 5 cut(s) 142, 405, 550, 636, 639
FokI GGATG 3 cut(s) 401, 584, 710
Fsp4HI GCNGC 5 cut(s) 142, 405, 550, 636, 639
FspBI CTAG 1 cut(s) 677
GlaI GCGC 1 cut(s) 112
GluI GCNGC 5 cut(s) 142, 405, 550, 636, 639
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 4 cut(s) 41, 162, 231, 333
HapII CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
HgaI GACGC 4 cut(s) 214, 332, 365, 473
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
Hin1II CATG 3 cut(s) 202, 259, 457
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 4 cut(s) 97, 322, 373, 418
HindII GTYRAC 4 cut(s) 97, 322, 373, 418
HinfI GANTC 3 cut(s) 4, 165, 655
HpaII CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
HphI GGTGA 3 cut(s) 169, 656, 777
Hpy166II GTNNAC 5 cut(s) 97, 151, 322, 373, 418
Hpy188I TCNGA 4 cut(s) 286, 426, 500, 794
Hpy8I GTNNAC 5 cut(s) 97, 151, 322, 373, 418
HpyAV CCTTC 8 cut(s) 23, 29, 44, 81, 562, 674, 698, 773
HpyCH4IV ACGT 4 cut(s) 99, 318, 369, 420
HpyCH4V TGCA 3 cut(s) 641, 776, 798
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 4 cut(s) 99, 318, 369, 420
Hsp92I GRCGYC 6 cut(s) 99, 111, 225, 324, 357, 369
Hsp92II CATG 3 cut(s) 202, 259, 457
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
KflI GGGWCCC 1 cut(s) 312
KroI GCCGGC 2 cut(s) 202, 359
KroNI GCCGGC 2 cut(s) 204, 361
Kzo9I GATC 4 cut(s) 129, 343, 690, 782
LmnI GCTCC 5 cut(s) 218, 249, 272, 300, 575
Lsp1109I GCAGC 3 cut(s) 561, 622, 625
LweI GCATC 1 cut(s) 785
MaeI CTAG 1 cut(s) 677
MaeII ACGT 4 cut(s) 99, 318, 369, 420
MalI GATC 4 cut(s) 131, 345, 692, 784
MboI GATC 4 cut(s) 129, 343, 690, 782
MboII GAAGA 4 cut(s) 19, 160, 271, 331
MhlI GDGCHC 1 cut(s) 86
MluCI AATT 1 cut(s) 618
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 2 cut(s) 147, 751
MnlI CCTC 9 cut(s) 35, 56, 115, 130, 147, 248, 420, 451, 756
Mph1103I ATGCAT 1 cut(s) 800
MroNI GCCGGC 2 cut(s) 202, 359
MroXI GAANNNNTTC 1 cut(s) 739
MseI TTAA 1 cut(s) 396
MspI CCGG 6 cut(s) 80, 108, 203, 309, 330, 360
MspR9I CCNGG 4 cut(s) 81, 310, 330, 732
Mva1269I GAATGC 1 cut(s) 798
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 2 cut(s) 204, 361
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 3 cut(s) 81, 310, 330
NdeI CATATG 1 cut(s) 643
NdeII GATC 4 cut(s) 129, 343, 690, 782
NgoMIV GCCGGC 2 cut(s) 202, 359
NlaIII CATG 3 cut(s) 202, 259, 457
NlaIV GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
NsiI ATGCAT 1 cut(s) 800
NspI RCATGY 1 cut(s) 202
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 378
PctI GAATGC 1 cut(s) 798
PdiI GCCGGC 2 cut(s) 204, 361
PdmI GAANNNNTTC 1 cut(s) 739
PfeI GAWTC 3 cut(s) 4, 165, 655
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 5 cut(s) 143, 406, 551, 637, 640
PluTI GGCGCC 1 cut(s) 114
PpuMI RGGWCCY 1 cut(s) 312
PshAI GACNNNNGTC 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 730
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 7 cut(s) 85, 112, 214, 245, 268, 313, 314
PspPI GGNCC 1 cut(s) 312
PspPPI RGGWCCY 1 cut(s) 312
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
SalI GTCGAC 3 cut(s) 95, 320, 371
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 5 cut(s) 142, 405, 550, 636, 639
Sau3AI GATC 4 cut(s) 129, 343, 690, 782
Sau96I GGNCC 1 cut(s) 312
ScaI AGTACT 1 cut(s) 492
ScrFI CCNGG 4 cut(s) 81, 310, 330, 732
SduI GDGCHC 1 cut(s) 86
SfaNI GCATC 1 cut(s) 785
SfcI CTRYAG 1 cut(s) 187
SfoI GGCGCC 1 cut(s) 112
SgrDI CGTCGACG 2 cut(s) 95, 320
SinI GGWCC 1 cut(s) 312
Sse9I AATT 1 cut(s) 618
SsiI CCGC 5 cut(s) 117, 142, 184, 216, 404
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 4 cut(s) 79, 308, 328, 730
StyI CCWWGG 1 cut(s) 474
TaiI ACGT 4 cut(s) 102, 321, 372, 423
TaqI TCGA 9 cut(s) 96, 171, 321, 342, 354, 366, 372, 381, 487
TasI AATT 1 cut(s) 618
TatI WGTACW 1 cut(s) 490
TauI GCSGC 2 cut(s) 144, 407
TfiI GAWTC 3 cut(s) 4, 165, 655
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 604
TseI GCWGC 3 cut(s) 549, 635, 638
TspDTI ATGAA 4 cut(s) 17, 272, 305, 581
TspGWI ACGGA 2 cut(s) 168, 527
TspRI CASTG 1 cut(s) 604
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 312
XceI RCATGY 1 cut(s) 202
XmiI GTMKAC 3 cut(s) 96, 321, 372
XmnI GAANNNNTTC 1 cut(s) 739
XspI CTAG 1 cut(s) 677
ZraI GACGTC 2 cut(s) 100, 370
ZrmI AGTACT 1 cut(s) 492
Zsp2I ATGCAT 1 cut(s) 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.