Rorug04G0014300

zinc-binding in reverse transcriptase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000519
Physical Location & Seq
Reverse (-)
2308032 .. 2311741
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0014300.1

Sequence Viewer

Length: 3294 bp
ATGGCTTTTCTGATCAATGCAGAAGCCAGTTCTTCATCTTCTTCTCTCCCACTTTCTTCTACTGATCACCAAAATCATTACACGTACGAGGTCTTCCTGAGTTTTCGAGGCGAGGATACGCACTTTAATTTTACGGATCATTTGTACAACGCTTTGTGTCGGAAGGGAATTGAGACCTTCAGAGATGCTGATAAGCTTAGAAGAGGAGAAGACGTATCACAAGAGCTCCTCAAAGCAATTGAGGAGTCCAGAGTTTCAATCGTCGTCTTCTCTCAAAACTATGCTTCCTCAACGTGGTGTTTGAATGAACTGGTCAAGATACTTGAATGCAGAAAATCAAAAAGACAAGAGGTCAGACCGGTTTTCTACAAAGTGGCTCCCTCAGATGTACGACACCAAAGAGGCGCCTTTGGTGACGCATTTGCTACGCTTGGTTGCAGATACGAGGATAGCATGGACAAATGGAAGACAGCTCTCAAGGAAGCAGCAGATTTGTCTGGATGGCCTTTGAAGGATGGCGAGTACGAGACCAAAGTCATCGACGACATTGTTGAGGAGTTGTCCGCCCAAGTGATAAACCCTTCATGTTACTTGGATGTTGCTGAACATCCAATTGGATTAGCGTCTTGTAGACAAGATGTCATCAGACTTTTACATGCCGAGGAAAATATTGTTCGCATGGTAGGCATATGGGGGCCTGGAGGAATAGGAAAGACCACAATTGCCAAAGATGTGTTTAATTCAATTTGCCATAAGTTTCAAAGTAGCTGTTTCTTGGCAGATGTTAGATCAAATGGCCTAGCCCAACTTCAAGAGACGCTTTTGTCGAATTTTTTAGACTCAACTTTAAAGGTGAGTAGTGTTGATCAAGGAGTCAATTTTATAAAGACAAGGATGCGACATAAAAAAGTTCTCTTAATCCTTGATGACGTGAGTGATTCTAGCCAATTACAGAAATTAGTTCCTTTCCATGATTGTTTTGGGCCTGGCAGTAGAATTCTCATAACAACAAGAGATAAACATTGTCTAACTGCTCATCAAGTTGATGAAGTATACGAGGTCAAAATGTTAGATGATGATCAAGCTTTGAAGTTGTTTAGCCTGAATGCATTCAGAGAAAATGGACCTCCAAGCGATCATCTAGAACTTGCACGATGTGCGGTACACTATGCCCAAGGCCTTCCCTTAGCGTTGATAGTTTTAGGCTCTCATCTATTTGGTAGAAGCAGAGAGGAGTGGGAAGCTACATTAGATAGTTGTAAAGGAGAAGATCCATACAACGAGATAAGAAACATTCTTAAAATAAGTTATGATGCTCTGGGAGTAGATTTACAAGGATATTTTCTTGATATCGCTTGTTTCTTTAAAGGGAAGCTTGTACACGATGTGAAACCAATACTAGAAGCTTGCTACGACCTCAAATCAGTGAATGGTATTGCACAACTCCAAGAAAAGGCCTTAATAAGGATTGACAATGAGGCTTTGATGGGTGGTTGGATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTGTATAAAAAGTCACCTAATGAACCCGGGAAACGCAGCAGAGTGTGGAGTGAAGAAGATGTCGACGACGTTCTAACAAATAATACAGGAACAAAGAAAGTTGTAGGCATTCAGGTCCCGTGGGGATTATCTACGATATCTTTGAATGCTAAAACCTTCTCAAAGATGAAGAATCTTAGATATATTTCTATGAAGAGGATGATGCAATATGAATCCTTTTATGGAGATATTGATTATCTCTCCAACCAGTTGAGGTGGCTTGATTGGCCACATTGTCCGTTGCAATGTTTTCCATCCAATTTTCATGCAAAGAAACTTGTAAATCTCAATATTTCTTGGAGTCGCGGAATCACACGACTATGGGAGGGACATAAGAATTTTTCAAGGCTAACATGTATGAATTTAGAGGGCTGTGAATCCCTAACAGAACTCCCAGCCTTAAGTGGAATCCCAAACTTGGAAGAGTTGAATTTAAGTGGCTGTAGATCCCTAACAGAACTCCCAGACTTGAGTGGAATCCCTAACTTGAAAAAGTTGAATTTAGATGGTTGTACATCCCTAAAGGAACTCCCAGACTTGAGTAGAATCCCTAACTTGAAAGAGTTGGATCTATTTGGGTGTACCAATTTAGTTGAGGTTCCTGATTCAATTCGATTCCTTCATAACCTTGAGACTCTTGATGCGAGTGGATGCTCAAAACTGGCTACATTTCCAAAAATTCCAGTAAAGATGGATTCCTTGAGACGAGTCTCTCTTGAAGACAGTGACATAAGAGAATTGGATGAGTCAATTGAAAATCTCATTGGGCTTGAAAGGTTGCATCTTACTGATTGCAAAAATCTCACAACTCTACCATGCAGCATTTATGGGTTGCACAATCTAAAGACTCTTTATGCGAGAGGATGCTCAAAACTGGCTACATTTCTAAAAATTCCAGTAAAAATGGATTCCTTGAGACTACTCACTCTTAAAGGCAGTGACATAAGAGAATTGGATGAGTCAATTGAAAATCTCATTGGGCTTGAATATTTGGATCTTACTGATTGCAAAAATCTCAGAACTCTACCGTGCAGCATTTATGGGTTGCAAAATTTATGGTTTCTTGATCTTGGTGAATGCTCAAAACTTGTCAGATTTCCAACAAATACCAAAATTTTGAATGTCGATAGTTGCTCACTATCACTTCCCAAGCTAGGTGTGTTCAGTATCGCAGGATGCAGTTTATTATCAGATTGTGATTTCCTGATGACTCTTGATTGCTGGGAAACATTACAGTGGCTTAATCTGTCAAGAAACAATTTTGTTAGTCTTCCTGCTTGCCTTACCAAATTTGTCAAGTTGCGGATTTTTGACTTGGAGGATTGCAAGAGACTTCGGGAAATTCCGGAGCTTCCACCAAACGTTAAACCAAAAATAAGAGGTTGTGAATTACTGGAGGAGAGATTCTCGACATTGACAAATCGTGGAGCTGTGATAGAGACGGCGTCGCCGTCAATTGAAGAGCCAAATCCGTCGTCATGGCTTTCTTTGTCTTCCGAAACTCCGAAATTACACCGCAGCGCGGAAGAACCATCCCCAACACCAGTGAATCCTCCGATGGCTATCTCCCAAATCCCCATGATCCCTGACCTGCAGACCCATCACCGTGGCTGGCGTTGGTGGCGTTGGTGGCTTTCGTCGGCGAATGACGATTATCACCATCCACAAGAGCAGCAGGAGCATCCGATTTCTCCGCAGCTCCCATCACTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

1097

Amino Acids

124.28

Weight (kDa)

5.72

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 29 - 190 6.4e-51 TIR domain
TIR_2 PF13676 31 - 126 6.2e-16 TIR domain
NB-ARC PF00931 214 - 373 4e-24 NB-ARC domain
WHD_ROQ1 PF23282 445 - 518 3.8e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_4 PF12799 664 - 707 1.5e-06 Leucine Rich repeats (2 copies)
LRR_Zer-1 PF25013 683 - 808 1.3e-07 Zer-1-like, Leucine-rich repeats
LRR_13 PF23286 732 - 822 8.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 830 - 889 3.8e-07 Leucine-rich repeat region
LRR_13 PF23286 837 - 977 1.1e-15 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 884
AasI GACNNNNNNGTC 1 cut(s) 823
Acc36I ACCTGC 1 cut(s) 3177
AccB1I GGYRCC 1 cut(s) 404
AccI GTMKAC 3 cut(s) 631, 1053, 1602
AccII CGCG 2 cut(s) 1884, 3101
AccIII TCCGGA 1 cut(s) 2923
AciI CCGC 7 cut(s) 564, 1160, 1884, 2881, 3094, 3101, 3272
AclI AACGTT 1 cut(s) 2940
AclWI GGATC 6 cut(s) 144, 1265, 2019, 2154, 2580, 3154
AcoI YGGCCR 1 cut(s) 1805
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 2 cut(s) 405, 3023
AdeI CACNNNGTG 2 cut(s) 1157, 1387
AfaI GTAC 8 cut(s) 86, 146, 390, 524, 1164, 1380, 2092, 2161
AfiI CCNNNNNNNGG 6 cut(s) 294, 1453, 1464, 1996, 2732, 3100
AflII CTTAAG 1 cut(s) 1978
AflIII ACRYGT 2 cut(s) 81, 1931
AgeI ACCGGT 1 cut(s) 358
AhdI GACNNNNNGTC 2 cut(s) 350, 638
AjiI CACGTC 1 cut(s) 931
AjnI CCWGG 2 cut(s) 697, 985
AjuI GAANNNNNNNTTGG 6 cut(s) 597, 629, 2325, 2357, 2538, 2570
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 9 cut(s) 167, 521, 809, 2204, 2275, 2293, 2488, 2902, 3011
AlwI GGATC 6 cut(s) 144, 1265, 2019, 2154, 2580, 3154
Ama87I CYCGRG 1 cut(s) 1565
Aor13HI TCCGGA 1 cut(s) 2923
AoxI GGCC 7 cut(s) 503, 695, 796, 983, 1177, 1455, 1805
ApeKI GCWGC 7 cut(s) 485, 1575, 2397, 2610, 3096, 3250, 3274
ArsI GACNNNNNNTTYG 2 cut(s) 3038, 3070
AsiGI ACCGGT 1 cut(s) 358
Asp700I GAANNNNTTC 1 cut(s) 1109
AspLEI GCGC 2 cut(s) 407, 3101
AspS9I GGNCC 4 cut(s) 695, 983, 1124, 1654
AsuC2I CCSGG 2 cut(s) 1566, 1567
AsuHPI GGTGA 7 cut(s) 59, 425, 865, 1545, 2663, 3173, 3227
AvaI CYCGRG 1 cut(s) 1565
AvaII GGWCC 2 cut(s) 1124, 1654
BalI TGGCCA 1 cut(s) 1807
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 1 cut(s) 228
BarI GAAGNNNNNNTAC 2 cut(s) 77, 109
BbsI GAAGAC 7 cut(s) 85, 216, 259, 473, 2304, 2840, 3063
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 7 cut(s) 497, 1587, 2409, 2622, 3108, 3262, 3286
BceAI ACGGC 2 cut(s) 3013, 3036
BciT130I CCWGG 2 cut(s) 699, 987
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 4 cut(s) 12, 64, 865, 1078
BcnI CCSGG 2 cut(s) 1566, 1567
BcoDI GTCTC 9 cut(s) 167, 521, 809, 2204, 2275, 2293, 2488, 2902, 3011
BfaI CTAG 5 cut(s) 800, 942, 1142, 1400, 2732
BfmI CTRYAG 2 cut(s) 2020, 3170
BfoI RGCGCY 1 cut(s) 408
BfrI CTTAAG 1 cut(s) 1978
BfuAI ACCTGC 1 cut(s) 3177
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 7 cut(s) 486, 1576, 2398, 2611, 3097, 3251, 3275
BlsI GCNGC 7 cut(s) 487, 1577, 2399, 2612, 3098, 3252, 3276
Bme1390I CCNGG 4 cut(s) 699, 987, 1566, 1567
Bme18I GGWCC 2 cut(s) 1124, 1654
BmeRI GACNNNNNGTC 2 cut(s) 350, 638
BmeT110I CYCGRG 1 cut(s) 1565
BmgBI CACGTC 1 cut(s) 931
BmgT120I GGNCC 4 cut(s) 695, 983, 1124, 1654
BmiI GGNNCC 5 cut(s) 378, 406, 696, 1656, 2178
BmrFI CCNGG 4 cut(s) 699, 987, 1566, 1567
BoxI GACNNNNGTC 1 cut(s) 533
BpiI GAAGAC 7 cut(s) 85, 216, 259, 473, 2304, 2840, 3063
BplI GAGNNNNNCTC 2 cut(s) 2969, 3001
BpmI CTGGAG 2 cut(s) 720, 2993
Bpu10I CCTNAGC 1 cut(s) 1186
BpuEI CTTGAG 6 cut(s) 461, 2068, 2137, 2228, 2299, 2512
BpuMI CCSGG 2 cut(s) 1566, 1567
BsaAI YACGTR 1 cut(s) 84
BsaBI GATNNNNATC 2 cut(s) 1077, 3140
BsaHI GRCGYC 2 cut(s) 405, 3023
BsaI GGTCTC 2 cut(s) 167, 521
BsaJI CCNNGG 5 cut(s) 660, 1174, 1565, 1658, 3184
BsaWI WCCGGW 2 cut(s) 358, 2923
BsaXI ACNNNNNCTCC 4 cut(s) 198, 210, 228, 240
Bsc4I CCNNNNNNNGG 6 cut(s) 294, 1453, 1464, 1996, 2732, 3100
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 9 cut(s) 27, 315, 1786, 2244, 2261, 2457, 2474, 2976, 3122
Bse3DI GCAATG 1 cut(s) 1829
Bse8I GATNNNNATC 2 cut(s) 1077, 3140
BseAI TCCGGA 1 cut(s) 2923
BseBI CCWGG 2 cut(s) 699, 987
BseDI CCNNGG 5 cut(s) 660, 1174, 1565, 1658, 3184
BseJI GATNNNNATC 2 cut(s) 1077, 3140
BseLI CCNNNNNNNGG 6 cut(s) 294, 1453, 1464, 1996, 2732, 3100
BseMI GCAATG 1 cut(s) 1829
BseMII CTCAG 3 cut(s) 89, 396, 2608
BseNI ACTGG 9 cut(s) 27, 315, 1786, 2244, 2261, 2457, 2474, 2976, 3122
BseRI GAGGAG 6 cut(s) 218, 219, 257, 569, 1247, 2990
BseXI GCAGC 7 cut(s) 497, 1587, 2409, 2622, 3108, 3262, 3286
BseYI CCCAGC 2 cut(s) 1972, 2799
BsgI GTGCAG 1 cut(s) 2629
Bsh1236I CGCG 2 cut(s) 1884, 3101
BshFI GGCC 7 cut(s) 505, 697, 798, 985, 1179, 1457, 1807
BshNI GGYRCC 1 cut(s) 404
BshTI ACCGGT 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 1565
BsiSI CCGG 3 cut(s) 359, 1566, 2924
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 1640, 1920
BslI CCNNNNNNNGG 6 cut(s) 294, 1453, 1464, 1996, 2732, 3100
BsmAI GTCTC 9 cut(s) 167, 521, 809, 2204, 2275, 2293, 2488, 2902, 3011
BsmBI CGTCTC 3 cut(s) 809, 2275, 3011
BsmFI GGGAC 2 cut(s) 1640, 1920
BsmI GAATGC 6 cut(s) 332, 1109, 1111, 1647, 1690, 2660
BsnI GGCC 7 cut(s) 505, 697, 798, 985, 1179, 1457, 1807
Bso31I GGTCTC 2 cut(s) 167, 521
BsoBI CYCGRG 1 cut(s) 1565
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 2923
Bsp1407I TGTACA 3 cut(s) 144, 1378, 2090
BspACI CCGC 7 cut(s) 564, 1160, 1884, 2881, 3094, 3101, 3272
BspANI GGCC 7 cut(s) 505, 697, 798, 985, 1179, 1457, 1807
BspCNI CTCAG 3 cut(s) 90, 395, 2607
BspEI TCCGGA 1 cut(s) 2923
BspFNI CGCG 2 cut(s) 1884, 3101
BspLI GGNNCC 5 cut(s) 378, 406, 696, 1656, 2178
BspMAI CTGCAG 1 cut(s) 3174
BspMI ACCTGC 1 cut(s) 3177
BspPI GGATC 6 cut(s) 144, 1265, 2019, 2154, 2580, 3154
BspQI GCTCTTC 1 cut(s) 3033
BspT107I GGYRCC 1 cut(s) 404
BspTI CTTAAG 1 cut(s) 1978
BspTNI GGTCTC 2 cut(s) 167, 521
BsrDI GCAATG 1 cut(s) 1829
BsrFI RCCGGY 1 cut(s) 358
BsrGI TGTACA 3 cut(s) 144, 1378, 2090
BsrI ACTGG 9 cut(s) 27, 315, 1786, 2244, 2261, 2457, 2474, 2976, 3122
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 5 cut(s) 660, 1174, 1565, 1658, 3184
BssNAI GTATAC 1 cut(s) 1054
BssNI GRCGYC 2 cut(s) 405, 3023
BssT1I CCWWGG 1 cut(s) 1174
Bst1107I GTATAC 1 cut(s) 1054
Bst2UI CCWGG 2 cut(s) 699, 987
Bst4CI ACNGT 4 cut(s) 2303, 2607, 2814, 3185
Bst6I CTCTTC 4 cut(s) 196, 1727, 1995, 3033
BstACI GRCGYC 2 cut(s) 405, 3023
BstAFI CTTAAG 1 cut(s) 1978
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstAUI TGTACA 3 cut(s) 144, 1378, 2090
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 1408, 2857, 3191
BstDEI CTNAG 6 cut(s) 98, 197, 382, 1186, 1715, 2594
BstDSI CCRYGG 2 cut(s) 1658, 3184
BstENI CCTNNNNNAGG 1 cut(s) 1462
BstFNI CGCG 2 cut(s) 1884, 3101
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 2 cut(s) 407, 3101
BstMAI GTCTC 9 cut(s) 167, 521, 809, 2204, 2275, 2293, 2488, 2902, 3011
BstMWI GCNNNNNNNGC 6 cut(s) 684, 1157, 1804, 3199, 3208, 3256
BstNI CCWGG 2 cut(s) 699, 987
BstNSI RCATGY 2 cut(s) 659, 1935
BstPAI GACNNNNGTC 1 cut(s) 533
BstSCI CCNGG 4 cut(s) 697, 985, 1564, 1565
BstSFI CTRYAG 2 cut(s) 2020, 3170
BstUI CGCG 2 cut(s) 1884, 3101
BstV1I GCAGC 7 cut(s) 497, 1587, 2409, 2622, 3108, 3262, 3286
BstV2I GAAGAC 7 cut(s) 85, 216, 259, 473, 2304, 2840, 3063
BstX2I RGATCY 4 cut(s) 1270, 2024, 2146, 2572
BstXI CCANNNNNNTGG 1 cut(s) 3185
BstYI RGATCY 4 cut(s) 1270, 2024, 2146, 2572
BstZ17I GTATAC 1 cut(s) 1054
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 7 cut(s) 505, 697, 798, 985, 1179, 1457, 1807
BtgI CCRYGG 2 cut(s) 1658, 3184
BtrI CACGTC 1 cut(s) 931
BtsI GCAGTG 1 cut(s) 2521
BtsIMutI CAGTG 5 cut(s) 1431, 2308, 2521, 2819, 3129
BveI ACCTGC 1 cut(s) 3177
Cac8I GCNNGC 3 cut(s) 1408, 2857, 3191
CfoI GCGC 2 cut(s) 407, 3101
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 4 cut(s) 695, 983, 1124, 1654
Cfr9I CCCGGG 1 cut(s) 1565
CseI GACGC 4 cut(s) 425, 612, 826, 3012
Csp6I GTAC 8 cut(s) 85, 145, 389, 523, 1163, 1379, 2091, 2160
CspAI ACCGGT 1 cut(s) 358
CviQI GTAC 8 cut(s) 85, 145, 389, 523, 1163, 1379, 2091, 2160
DdeI CTNAG 6 cut(s) 98, 197, 382, 1186, 1715, 2594
DinI GGCGCC 1 cut(s) 406
DraI TTTAAA 2 cut(s) 849, 1366
DraIII CACNNNGTG 2 cut(s) 1157, 1387
DrdI GACNNNNNNGTC 1 cut(s) 823
DriI GACNNNNNGTC 2 cut(s) 350, 638
DseDI GACNNNNNNGTC 1 cut(s) 823
EaeI YGGCCR 1 cut(s) 1805
Eam1104I CTCTTC 4 cut(s) 196, 1727, 1995, 3033
Eam1105I GACNNNNNGTC 2 cut(s) 350, 638
EarI CTCTTC 4 cut(s) 196, 1727, 1995, 3033
EciI GGCGGA 1 cut(s) 553
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 1174
Eco147I AGGCCT 2 cut(s) 1179, 1457
Eco24I GRGCYC 1 cut(s) 228
Eco31I GGTCTC 2 cut(s) 167, 521
Eco32I GATATC 2 cut(s) 1351, 1677
Eco47I GGWCC 2 cut(s) 1124, 1654
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 163
Eco88I CYCGRG 1 cut(s) 1565
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 1462
EcoO109I RGGNCCY 2 cut(s) 695, 1654
EcoRI GAATTC 1 cut(s) 996
EcoRII CCWGG 2 cut(s) 697, 985
EcoRV GATATC 2 cut(s) 1351, 1677
EcoT14I CCWWGG 1 cut(s) 1174
EcoT22I ATGCAT 2 cut(s) 1111, 1509
EcoT38I GRGCYC 1 cut(s) 228
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 1174
Esp3I CGTCTC 3 cut(s) 809, 2275, 3011
FalI AAGNNNNNCTT 4 cut(s) 804, 836, 1359, 1391
FaqI GGGAC 2 cut(s) 1640, 1920
FauNDI CATATG 1 cut(s) 689
FbaI TGATCA 4 cut(s) 12, 64, 865, 1078
FblI GTMKAC 3 cut(s) 631, 1053, 1602
Fnu4HI GCNGC 7 cut(s) 486, 1576, 2398, 2611, 3097, 3251, 3275
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 7 cut(s) 486, 1576, 2398, 2611, 3097, 3251, 3275
FspBI CTAG 5 cut(s) 800, 942, 1142, 1400, 2732
GlaI GCGC 2 cut(s) 406, 3100
GluI GCNGC 7 cut(s) 486, 1576, 2398, 2611, 3097, 3251, 3275
GsaI CCCAGC 2 cut(s) 1976, 2803
GsuI CTGGAG 2 cut(s) 720, 2993
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 7 cut(s) 505, 697, 798, 985, 1179, 1457, 1807
HapII CCGG 3 cut(s) 359, 1566, 2924
HgaI GACGC 4 cut(s) 425, 612, 826, 3012
HhaI GCGC 2 cut(s) 407, 3101
Hin1I GRCGYC 2 cut(s) 405, 3023
Hin6I GCGC 2 cut(s) 405, 3099
HinP1I GCGC 2 cut(s) 405, 3099
HincII GTYRAC 1 cut(s) 1603
HindII GTYRAC 1 cut(s) 1603
HindIII AAGCTT 4 cut(s) 194, 1083, 1373, 1404
HpaII CCGG 3 cut(s) 359, 1566, 2924
HphI GGTGA 7 cut(s) 59, 425, 865, 1545, 2663, 3173, 3227
Hpy166II GTNNAC 6 cut(s) 632, 1054, 1165, 1381, 1603, 2160
Hpy8I GTNNAC 6 cut(s) 632, 1054, 1165, 1381, 1603, 2160
Hpy99I CGWCG 7 cut(s) 266, 545, 1607, 1610, 3028, 3055, 3220
HpyAV CCTTC 7 cut(s) 157, 187, 505, 591, 1190, 1705, 2207
HpyCH4III ACNGT 4 cut(s) 2303, 2607, 2814, 3185
HpyCH4IV ACGT 6 cut(s) 83, 213, 293, 930, 1608, 2940
HpyF10VI GCNNNNNNNGC 6 cut(s) 684, 1157, 1804, 3199, 3208, 3256
HpyF3I CTNAG 6 cut(s) 98, 197, 382, 1186, 1715, 2594
HpySE526I ACGT 6 cut(s) 83, 213, 293, 930, 1608, 2940
Hsp92I GRCGYC 2 cut(s) 405, 3023
HspAI GCGC 2 cut(s) 405, 3099
KasI GGCGCC 1 cut(s) 404
Kpn2I TCCGGA 1 cut(s) 2923
Ksp22I TGATCA 4 cut(s) 12, 64, 865, 1078
LguI GCTCTTC 1 cut(s) 3033
LmnI GCTCC 6 cut(s) 231, 382, 2926, 3005, 3256, 3282
Lsp1109I GCAGC 7 cut(s) 497, 1587, 2409, 2622, 3108, 3262, 3286
MaeI CTAG 5 cut(s) 800, 942, 1142, 1400, 2732
MaeII ACGT 6 cut(s) 83, 213, 293, 930, 1608, 2940
MaeIII GTNAC 5 cut(s) 413, 587, 1551, 2303, 2516
MfeI CAATTG 6 cut(s) 237, 612, 720, 2328, 2541, 3033
MflI RGATCY 4 cut(s) 1270, 2024, 2146, 2572
MhlI GDGCHC 1 cut(s) 228
MlsI TGGCCA 1 cut(s) 1807
MluNI TGGCCA 1 cut(s) 1807
Mly113I GGCGCC 1 cut(s) 405
MmeI TCCRAC 5 cut(s) 140, 1475, 1806, 2124, 2702
Mox20I TGGCCA 1 cut(s) 1807
Mph1103I ATGCAT 2 cut(s) 1111, 1509
MroI TCCGGA 1 cut(s) 2923
MroXI GAANNNNTTC 1 cut(s) 1109
MscI TGGCCA 1 cut(s) 1807
MslI CAYNNNNRTG 3 cut(s) 1897, 2812, 3183
Msp20I TGGCCA 1 cut(s) 1807
MspCI CTTAAG 1 cut(s) 1978
MspI CCGG 3 cut(s) 359, 1566, 2924
MspR9I CCNGG 4 cut(s) 699, 987, 1566, 1567
MunI CAATTG 6 cut(s) 237, 612, 720, 2328, 2541, 3033
Mva1269I GAATGC 6 cut(s) 332, 1109, 1111, 1647, 1690, 2660
MvaI CCWGG 2 cut(s) 699, 987
MvnI CGCG 2 cut(s) 1884, 3101
MwoI GCNNNNNNNGC 6 cut(s) 684, 1157, 1804, 3199, 3208, 3256
NarI GGCGCC 1 cut(s) 405
NciI CCSGG 2 cut(s) 1566, 1567
NdeI CATATG 1 cut(s) 689
NlaIV GGNNCC 5 cut(s) 378, 406, 696, 1656, 2178
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 4 cut(s) 413, 1551, 2303, 2516
NsiI ATGCAT 2 cut(s) 1111, 1509
NspI RCATGY 2 cut(s) 659, 1935
PceI AGGCCT 2 cut(s) 1179, 1457
PciI ACATGT 1 cut(s) 1931
PciSI GCTCTTC 1 cut(s) 3033
PcsI WCGNNNNNNNCGW 2 cut(s) 824, 3026
PctI GAATGC 6 cut(s) 332, 1109, 1111, 1647, 1690, 2660
PdmI GAANNNNTTC 1 cut(s) 1109
Pfl23II CGTACG 1 cut(s) 84
PflFI GACNNNGTC 1 cut(s) 3022
PinAI ACCGGT 1 cut(s) 358
PkrI GCNGC 7 cut(s) 487, 1577, 2399, 2612, 3098, 3252, 3276
PluTI GGCGCC 1 cut(s) 408
Ppu21I YACGTR 1 cut(s) 84
PpuMI RGGWCCY 1 cut(s) 1654
PscI ACATGT 1 cut(s) 1931
PshAI GACNNNNGTC 1 cut(s) 533
PsiI TTATAA 1 cut(s) 884
Psp124BI GAGCTC 1 cut(s) 228
Psp1406I AACGTT 1 cut(s) 2940
Psp5II RGGWCCY 1 cut(s) 1654
Psp6I CCWGG 2 cut(s) 697, 985
PspFI CCCAGC 2 cut(s) 1972, 2799
PspGI CCWGG 2 cut(s) 697, 985
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 5 cut(s) 378, 406, 696, 1656, 2178
PspPI GGNCC 4 cut(s) 695, 983, 1124, 1654
PspPPI RGGWCCY 1 cut(s) 1654
PstI CTGCAG 1 cut(s) 3174
PsuI RGATCY 4 cut(s) 1270, 2024, 2146, 2572
PsyI GACNNNGTC 1 cut(s) 3022
RsaI GTAC 8 cut(s) 86, 146, 390, 524, 1164, 1380, 2092, 2161
RsaNI GTAC 8 cut(s) 85, 145, 389, 523, 1163, 1379, 2091, 2160
RseI CAYNNNNRTG 3 cut(s) 1897, 2812, 3183
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 1601
SapI GCTCTTC 1 cut(s) 3033
SatI GCNGC 7 cut(s) 486, 1576, 2398, 2611, 3097, 3251, 3275
Sau96I GGNCC 4 cut(s) 695, 983, 1124, 1654
ScrFI CCNGG 4 cut(s) 699, 987, 1566, 1567
SduI GDGCHC 1 cut(s) 228
SfcI CTRYAG 2 cut(s) 2020, 3170
SfoI GGCGCC 1 cut(s) 406
SinI GGWCC 2 cut(s) 1124, 1654
SmaI CCCGGG 1 cut(s) 1567
SmiMI CAYNNNNRTG 3 cut(s) 1897, 2812, 3183
SmlI CTYRAG 7 cut(s) 476, 1978, 2047, 2116, 2207, 2278, 2491
SmoI CTYRAG 7 cut(s) 476, 1978, 2047, 2116, 2207, 2278, 2491
SseBI AGGCCT 2 cut(s) 1179, 1457
SsiI CCGC 7 cut(s) 564, 1160, 1884, 2881, 3094, 3101, 3272
SspDI GGCGCC 1 cut(s) 404
SspI AATATT 3 cut(s) 670, 1870, 2567
SspMI CTAG 5 cut(s) 800, 942, 1142, 1400, 2732
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 2 cut(s) 1179, 1457
StyD4I CCNGG 4 cut(s) 697, 985, 1564, 1565
StyI CCWWGG 1 cut(s) 1174
TaaI ACNGT 4 cut(s) 2303, 2607, 2814, 3185
TaiI ACGT 6 cut(s) 86, 216, 296, 933, 1611, 2943
TaqI TCGA 7 cut(s) 106, 540, 827, 1602, 2191, 2703, 2987
TatI WGTACW 3 cut(s) 144, 1378, 2090
TscAI CASTG 5 cut(s) 1431, 2308, 2521, 2819, 3129
TseFI GTSAC 4 cut(s) 413, 1551, 2303, 2516
TseI GCWGC 7 cut(s) 485, 1575, 2397, 2610, 3096, 3250, 3274
Tsp45I GTSAC 4 cut(s) 413, 1551, 2303, 2516
TspGWI ACGGA 3 cut(s) 149, 1806, 3039
TspMI CCCGGG 1 cut(s) 1565
TspRI CASTG 5 cut(s) 1431, 2308, 2521, 2819, 3129
Tth111I GACNNNGTC 1 cut(s) 3022
Vha464I CTTAAG 1 cut(s) 1978
VpaK11BI GGWCC 2 cut(s) 1124, 1654
XagI CCTNNNNNAGG 1 cut(s) 1462
XbaI TCTAGA 1 cut(s) 1141
XceI RCATGY 2 cut(s) 659, 1935
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XmaI CCCGGG 1 cut(s) 1565
XmiI GTMKAC 3 cut(s) 631, 1053, 1602
XmnI GAANNNNTTC 1 cut(s) 1109
XspI CTAG 5 cut(s) 800, 942, 1142, 1400, 2732
Zsp2I ATGCAT 2 cut(s) 1111, 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.