FvH4_1g27751

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000519
Physical Location & Seq
Forward (+)
19353325 .. 19353836
512 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g27751.t1

Sequence Viewer

Length: 393 bp
ATGATGCTTTATCAACAGCCAATTTACACTGATGGACACCAGCATCTGAGGGATGTGTTAAACTTAACACCGATGGAAGTTTTTTACCTAATAAGATTAAAGGTGGTACAGGAGACCCTAAAGGTGAGTAATGTAGAAGTGGAAACTGACTGTCTAGAAGCTGTTGCTGCTATCAGCTCCAAGACTCATGAAGGTATGTTAGAAGCCGGTCTCATTGATGATATAAAGGAAATTCTGAAAACTTTTGCATCAGTTTCTGTAAAGCATGTATTAAGGCTATGTAACAATGTAGCACATAGGTTAGCAGCTTTAGCCTATGAAGATAAAGTCAGTTCGATCTGGCTTCAAAGACCAGTTTGCATACTGGATGTACTGGAAACTGATTGTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

131

Amino Acids

14.72

Weight (kDa)

5.26

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 40 - 104 6.3e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000519)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27751 FvH4_2g10620 FvH4_4g13701 FvH4_4g17370 FvH4_6g49051
pyrus_communis pycom11g18020
rosa_chinensis RchiOBHm_Chr1g0331041 RchiOBHm_Chr2g0088901 RchiOBHm_Chr2g0100641 RchiOBHm_Chr6g0281891
rosa_laevigata RLG00000011791 RLG00000028155 RLG00000035981
rosa_multiflora Rmu_co8368635.1_g000001 Rmu_co8370105.1_g000001 Rmu_sc0000850.1_g000010 Rmu_sc0001374.1_g000060 Rmu_sc0001654.1_g000024 Rmu_sc0001840.1_g000023 Rmu_sc0001900.1_g000032 Rmu_sc0002687.1_g000008 Rmu_sc0003133.1_g000026 Rmu_sc0003252.1_g000002 Rmu_sc0004206.1_g000011 Rmu_sc0004666.1_g000002 Rmu_sc0006255.1_g000004 Rmu_sc0006812.1_g000001 Rmu_sc0007025.1_g000012 Rmu_sc0007767.1_g000020 Rmu_sc0007833.1_g000004 Rmu_sc0007848.1_g000020 Rmu_sc0007883.1_g000010 Rmu_sc0008270.1_g000003 Rmu_sc0012097.1_g000011 Rmu_sc0014445.1_g000002 Rmu_sc0023292.1_g000001
rosa_roxburghii Rroxscaffold_1G00021670 Rroxscaffold_1G00027660 Rroxscaffold_1G00056090 Rroxscaffold_2G00111250 Rroxscaffold_2G00111320 Rroxscaffold_3G00248390 Rroxscaffold_6G00394880 Rroxscaffold_7G00192450
rosa_rugosa Rorug01G0071500 Rorug01G0183900 Rorug01G0242400 Rorug02G0045300 Rorug02G0051000 Rorug02G0061700 Rorug02G0091500 Rorug02G0091600 Rorug02G0091700 Rorug02G0091800 Rorug02G0284700 Rorug02G0335100 Rorug02G0336900 Rorug02G0405500.1 Rorug02G0498300 Rorug03G0173700 Rorug03G0228900 Rorug03G0256300 Rorug04G0014300 Rorug04G0130400 Rorug04G0138100 Rorug04G0138100 Rorug04G0150400.1 Rorug05G0000600 Rorug05G0160400 Rorug05G0165600 Rorug05G0378500 Rorug06G0072000 Rorug06G0107900 Rorug06G0268700 Rorug07G0015500 Rorug07G0266300 Rorug07G0274500
rosa_samantha Rh2AG439400 Rh2AG561100 Rh2DG459400 Rh3AG236100 Rh4BG212500 Rh6AG046300 Rh6BG073000 Rh7DG249100
rosa_wichuraiana Rw2G010910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 231
AfaI GTAC 2 cut(s) 108, 372
AgsI TTSAA 1 cut(s) 347
AluBI AGCT 3 cut(s) 161, 177, 308
AluI AGCT 3 cut(s) 161, 177, 308
Alw26I GTCTC 2 cut(s) 107, 215
AlwNI CAGNNNCTG 2 cut(s) 46, 257
ApeKI GCWGC 2 cut(s) 167, 305
ApoI RAATTY 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 136
BbvI GCAGC 2 cut(s) 154, 317
BccI CCATC 2 cut(s) 26, 67
BcoDI GTCTC 2 cut(s) 107, 215
BfaI CTAG 1 cut(s) 155
BisI GCNGC 2 cut(s) 168, 306
BlsI GCNGC 2 cut(s) 169, 307
BmsI GCATC 2 cut(s) 52, 257
BsaI GGTCTC 2 cut(s) 107, 215
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 3 cut(s) 353, 369, 378
BseGI GGATG 2 cut(s) 58, 373
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 3 cut(s) 353, 369, 378
BseXI GCAGC 2 cut(s) 154, 317
BsiSI CCGG 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 107, 215
Bso31I GGTCTC 2 cut(s) 107, 215
Bsp143I GATC 1 cut(s) 336
BspCNI CTCAG 1 cut(s) 39
BspHI TCATGA 1 cut(s) 187
BspTNI GGTCTC 2 cut(s) 107, 215
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 3 cut(s) 353, 369, 378
BssAI RCCGGY 1 cut(s) 206
BssMI GATC 1 cut(s) 336
Bst4CI ACNGT 1 cut(s) 152
BstDEI CTNAG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 58, 373
BstKTI GATC 1 cut(s) 339
BstMAI GTCTC 2 cut(s) 107, 215
BstMBI GATC 1 cut(s) 336
BstMWI GCNNNNNNNGC 2 cut(s) 167, 311
BstNSI RCATGY 1 cut(s) 269
BstV1I GCAGC 2 cut(s) 154, 317
BtsCI GGATG 2 cut(s) 58, 373
BtsIMutI CAGTG 1 cut(s) 27
CaiI CAGNNNCTG 2 cut(s) 46, 257
CciI TCATGA 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 206
Csp6I GTAC 2 cut(s) 107, 371
CviAII CATG 2 cut(s) 188, 266
CviJI RGCY 8 cut(s) 19, 161, 177, 206, 277, 308, 314, 343
CviKI_1 RGCY 8 cut(s) 19, 161, 177, 206, 277, 308, 314, 343
CviQI GTAC 2 cut(s) 107, 371
DdeI CTNAG 1 cut(s) 47
DpnI GATC 1 cut(s) 338
DpnII GATC 1 cut(s) 336
Eco31I GGTCTC 2 cut(s) 107, 215
FaeI CATG 2 cut(s) 191, 269
FaiI YATR 8 cut(s) 189, 197, 224, 267, 280, 297, 318, 362
FatI CATG 2 cut(s) 187, 265
Fnu4HI GCNGC 2 cut(s) 168, 306
FokI GGATG 2 cut(s) 65, 380
Fsp4HI GCNGC 2 cut(s) 168, 306
FspBI CTAG 1 cut(s) 155
GluI GCNGC 2 cut(s) 168, 306
HapII CCGG 1 cut(s) 207
Hin1II CATG 2 cut(s) 191, 269
HinfI GANTC 1 cut(s) 184
HpaII CCGG 1 cut(s) 207
HphI GGTGA 1 cut(s) 136
Hpy188I TCNGA 2 cut(s) 48, 237
Hpy188III TCNNGA 2 cut(s) 155, 188
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4V TGCA 2 cut(s) 248, 360
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 311
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 2 cut(s) 191, 269
Kzo9I GATC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 182
LpnPI CCDG 7 cut(s) 53, 95, 220, 325, 350, 359, 366
Lsp1109I GCAGC 2 cut(s) 154, 317
LweI GCATC 2 cut(s) 52, 257
MaeI CTAG 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 281
MalI GATC 1 cut(s) 338
MboI GATC 1 cut(s) 336
MboII GAAGA 1 cut(s) 332
MluCI AATT 2 cut(s) 21, 231
MlyI GAGTC 1 cut(s) 178
MnlI CCTC 1 cut(s) 42
MseI TTAA 4 cut(s) 59, 65, 98, 272
MspI CCGG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 167, 311
NdeII GATC 1 cut(s) 336
NlaIII CATG 2 cut(s) 191, 269
NspI RCATGY 1 cut(s) 269
PagI TCATGA 1 cut(s) 187
PkrI GCNGC 2 cut(s) 169, 307
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PstNI CAGNNNCTG 2 cut(s) 46, 257
RsaI GTAC 2 cut(s) 108, 372
RsaNI GTAC 2 cut(s) 107, 371
SaqAI TTAA 4 cut(s) 59, 65, 98, 272
SatI GCNGC 2 cut(s) 168, 306
Sau3AI GATC 1 cut(s) 336
SchI GAGTC 1 cut(s) 178
SetI ASST 8 cut(s) 90, 105, 126, 163, 179, 196, 302, 310
SfaNI GCATC 2 cut(s) 52, 257
Sse9I AATT 2 cut(s) 21, 231
SspMI CTAG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 152
TaqI TCGA 1 cut(s) 335
TasI AATT 2 cut(s) 21, 231
TatI WGTACW 1 cut(s) 370
Tru1I TTAA 4 cut(s) 59, 65, 98, 272
Tru9I TTAA 4 cut(s) 59, 65, 98, 272
TscAI CASTG 1 cut(s) 34
TseI GCWGC 2 cut(s) 167, 305
TspDTI ATGAA 2 cut(s) 204, 333
TspRI CASTG 1 cut(s) 34
XapI RAATTY 1 cut(s) 231
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 269
XspI CTAG 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.