AT3G13060

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001677
Physical Location & Seq
Forward (+)
4180154 .. 4183975
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G13060.5

Sequence Viewer

Length: 1656 bp
ATGGCAACGACTCAATCACACACCTCAGATCTTACTTCAGAGGAGCAACCTGCTAGTTTGGACATCATGAAGGAGCAGACTGTCCCGGCAAACAACGAGACATCAGCCTCTTTTAAGTCCTCCCAGGAAGCTCCAGTTGTAGTTCATCCAGCAAAAGTTGCTCCACTTACTGGACCTTATGGTTTAGCCGGTGATTTTGCCGGACACTTACCTAGCAGCATCCTCTCTCCTCAAGCACAAGGCTTTTACTATAGAGGTTATGAAAACCCCACAGGCGAATGGGACGAGTATTCCTCGTATGTCAATGTTGAAGGATTGGACATCACATCTCCTGTTGGCTTCAATGAGAATGCTTCTCTGGTCTACCAAACGGGATATGGATACAACCCTCAAATGCCATATGGGCCATATTCCCCTGCAGCAAGTCCCTTGCCTTCTGAGGGACAGTTGTATTCTCCACAACAGTTTCCGTTTTCAGGGGCATCACCGTATTACCAGCAGGTAGTTCCTCCTAGCATGCAATATATTACTTCTCCAACTCAGCCCGAGCTCACCAGCCTTGTCGGTGTTGACCAACAAGGTGATAACATCGGACCACGCCAATCCTACCATCCTCATCCCATTGGACCGTTTAATGGAAACCAGCCAAACCTTGGTTTTCCTGAATGGCAGCAAGGTTTTGACGGGGGAATATGGTCGGATTGGTCCAAGCCTTCTGATATGCACAGGCATTCTTCTTCTATTTCTCCGGCTTTATCTCCTCAGCCACTCGGCTCATATGGATCATATGGCCAGAACATACCCATGGGGTCACAGCGACAGAGATCATTTTACGGTTTTGGATCTGGTTCGAACTCTTATAACAGAGGTTATATGCATAGTGGTGGTCGTGGCCAGGGTTCTAACTATGGAAGTAGACTAATTTCTAATGTGGGTATGGGAAACCAAGGCTGGATTGGGGTTGACAACAGTCGAGGGCGTGGAAGGGTTAGTGATCCATCTTTGGGTGGTGCTTATAATGGTACTTTTGATATCCTCAATGAGCAAAATCGAGGACCAAGGGCTTCAAAGCCCAAGACTCAGGTCTTAGAGGAGCTTGATTCTGCTGCTGATTCTAAGAAAAATAACAAAGGCAGTGCAAAGGAGCACGAGGAATCCAACAACGCAGACTTTGTCACCGACTACACTAACGCCAAGTTGTTCATCATCAAGTCTTACAGTGAAGACAATGTTCACAAAAGCATCAAATACAATGTATGGGCTAGCACCCCTAATGGCAACAAAAAGCTTGACGCTGCTTATCGTGAGGCCAAGGATGAGAAAGAACCGTGTCCACTTTTTCTTCTGTTCTCGGTAAATGCGAGCTCTCAATTCTGTGGTGTAGCAGAGATGGTTGGACCTGTTGATTTTGAGAAGAGCGTTGATTACTGGCAACAAGATAAATGGAGTGGACAGTTCCCAGTGAAGTGGCACATTATCAAAGATGTTCCAAACAGCCAGTTCCGCCACATTATATTGGAAAACAATGACAATAAGCCTGTGACTAATAGTCGAGACACCCAAGAAGTAAGTAGCAATTTGTGCCTATCAAGCTTTCTTGTCCATAAGATACCTCTTTTAAGCCACACCATAACCACAACCAAAAACTACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

551

Amino Acids

60.56

Weight (kDa)

5.71

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YTH PF04146 399 - 452 2.1e-13 YT521-B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 861, 1017
Acc36I ACCTGC 2 cut(s) 58, 490
AccB7I CCANNNNNTGG 2 cut(s) 170, 653
AccI GTMKAC 2 cut(s) 363, 916
AciI CCGC 1 cut(s) 1504
AclWI GGATC 3 cut(s) 790, 850, 989
AcoI YGGCCR 2 cut(s) 790, 892
AcuI CTGAAG 1 cut(s) 21
AfaI GTAC 1 cut(s) 1024
AfiI CCNNNNNNNGG 6 cut(s) 170, 440, 476, 635, 653, 1004
AgsI TTSAA 3 cut(s) 311, 343, 1068
AhdI GACNNNNNGTC 1 cut(s) 1548
AjnI CCWGG 2 cut(s) 123, 894
AluBI AGCT 6 cut(s) 131, 550, 1096, 1288, 1365, 1593
AluI AGCT 6 cut(s) 131, 550, 1096, 1288, 1365, 1593
Alw21I GWGCWC 3 cut(s) 552, 1149, 1367
Alw26I GTCTC 2 cut(s) 92, 1548
AlwI GGATC 3 cut(s) 790, 850, 989
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 4 cut(s) 404, 790, 892, 1308
ApeKI GCWGC 5 cut(s) 216, 419, 670, 1106, 1295
AspS9I GGNCC 7 cut(s) 173, 404, 593, 626, 705, 1055, 1397
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 5 cut(s) 203, 477, 544, 593, 1168
AsuII TTCGAA 1 cut(s) 851
AsuNHI GCTAGC 1 cut(s) 1262
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 6 cut(s) 173, 593, 626, 705, 1055, 1397
BalI TGGCCA 2 cut(s) 792, 894
BanII GRGCYC 2 cut(s) 552, 1367
BauI CACGAG 1 cut(s) 1148
BbsI GAAGAC 1 cut(s) 1230
Bbv12I GWGCWC 3 cut(s) 552, 1149, 1367
BbvCI CCTCAGC 1 cut(s) 762
BbvI GCAGC 5 cut(s) 228, 431, 682, 1093, 1282
BccI CCATC 3 cut(s) 618, 1006, 1384
BciT130I CCWGG 2 cut(s) 125, 896
BciVI GTATCC 1 cut(s) 374
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 92, 1548
BfaI CTAG 4 cut(s) 54, 213, 513, 1263
BfmI CTRYAG 3 cut(s) 250, 417, 1648
BfuAI ACCTGC 2 cut(s) 58, 490
BfuI GTATCC 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 403
BglII AGATCT 1 cut(s) 28
BisI GCNGC 5 cut(s) 217, 420, 671, 1107, 1296
BlsI GCNGC 5 cut(s) 218, 421, 672, 1108, 1297
Bme1390I CCNGG 3 cut(s) 86, 125, 896
Bme18I GGWCC 6 cut(s) 173, 593, 626, 705, 1055, 1397
BmeRI GACNNNNNGTC 1 cut(s) 1548
BmeT110I CYCGRG 1 cut(s) 545
BmgT120I GGNCC 7 cut(s) 173, 404, 593, 626, 705, 1055, 1397
BmrFI CCNGG 3 cut(s) 86, 125, 896
BmrI ACTGGG 1 cut(s) 1454
BmsI GCATC 3 cut(s) 228, 491, 1251
BmtI GCTAGC 1 cut(s) 1266
BmuI ACTGGG 1 cut(s) 1454
BoxI GACNNNNGTC 2 cut(s) 969, 1082
BpiI GAAGAC 1 cut(s) 1230
BplI GAGNNNNNCTC 2 cut(s) 278, 310
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 762
Bpu14I TTCGAA 1 cut(s) 851
BpuEI CTTGAG 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 86
BsaJI CCNNGG 7 cut(s) 123, 652, 804, 895, 946, 1058, 1311
Bsc4I CCNNNNNNNGG 6 cut(s) 170, 440, 476, 635, 653, 1004
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 5 cut(s) 134, 175, 1433, 1460, 1498
BseBI CCWGG 2 cut(s) 125, 896
BseDI CCNNGG 7 cut(s) 123, 652, 804, 895, 946, 1058, 1311
BseGI GGATG 5 cut(s) 145, 219, 610, 616, 1321
BseLI CCNNNNNNNGG 6 cut(s) 170, 440, 476, 635, 653, 1004
BseMII CTCAG 5 cut(s) 39, 429, 554, 776, 1094
BseNI ACTGG 5 cut(s) 134, 175, 1433, 1460, 1498
BseRI GAGGAG 4 cut(s) 56, 219, 750, 1106
BseXI GCAGC 5 cut(s) 228, 431, 682, 1093, 1282
BshFI GGCC 4 cut(s) 406, 792, 894, 1310
BsiHKAI GWGCWC 3 cut(s) 552, 1149, 1367
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 4 cut(s) 86, 189, 201, 749
BslFI GGGAC 4 cut(s) 68, 296, 411, 456
BslI CCNNNNNNNGG 6 cut(s) 170, 440, 476, 635, 653, 1004
BsmAI GTCTC 2 cut(s) 92, 1548
BsmFI GGGAC 4 cut(s) 68, 296, 411, 456
BsmI GAATGC 2 cut(s) 355, 730
BsnI GGCC 4 cut(s) 406, 792, 894, 1310
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 851
Bsp1286I GDGCHC 3 cut(s) 552, 1149, 1367
Bsp143I GATC 5 cut(s) 28, 782, 824, 842, 994
Bsp19I CCATGG 1 cut(s) 804
BspACI CCGC 1 cut(s) 1504
BspANI GGCC 4 cut(s) 406, 792, 894, 1310
BspCNI CTCAG 5 cut(s) 38, 430, 553, 775, 1093
BspHI TCATGA 1 cut(s) 66
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 2 cut(s) 58, 490
BspOI GCTAGC 1 cut(s) 1266
BspPI GGATC 3 cut(s) 790, 850, 989
BspQI GCTCTTC 1 cut(s) 1409
BspT104I TTCGAA 1 cut(s) 851
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 5 cut(s) 134, 175, 1433, 1460, 1498
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 7 cut(s) 123, 652, 804, 895, 946, 1058, 1311
BssMI GATC 5 cut(s) 28, 782, 824, 842, 994
BssSI CACGAG 1 cut(s) 1148
BssT1I CCWWGG 5 cut(s) 652, 804, 946, 1058, 1311
Bst2BI CACGAG 1 cut(s) 1148
Bst2UI CCWGG 2 cut(s) 125, 896
Bst6I CTCTTC 1 cut(s) 1409
BstAPI GCANNNNNTGC 2 cut(s) 158, 1581
BstBI TTCGAA 1 cut(s) 851
BstC8I GCNNGC 3 cut(s) 518, 1264, 1363
BstDEI CTNAG 7 cut(s) 25, 438, 540, 762, 1080, 1087, 1116
BstDSI CCRYGG 1 cut(s) 804
BstF5I GGATG 5 cut(s) 145, 219, 610, 616, 1321
BstKTI GATC 5 cut(s) 31, 785, 827, 845, 997
BstMAI GTCTC 2 cut(s) 92, 1548
BstMBI GATC 5 cut(s) 28, 782, 824, 842, 994
BstMWI GCNNNNNNNGC 5 cut(s) 158, 403, 1503, 1581, 1590
BstNI CCWGG 2 cut(s) 125, 896
BstNSI RCATGY 1 cut(s) 520
BstPAI GACNNNNGTC 2 cut(s) 969, 1082
BstSCI CCNGG 3 cut(s) 84, 123, 894
BstSFI CTRYAG 3 cut(s) 250, 417, 1648
BstV1I GCAGC 5 cut(s) 228, 431, 682, 1093, 1282
BstV2I GAAGAC 1 cut(s) 1230
BstX2I RGATCY 2 cut(s) 28, 842
BstXI CCANNNNNNTGG 1 cut(s) 1467
BstYI RGATCY 2 cut(s) 28, 842
BsuI GTATCC 1 cut(s) 374
BsuRI GGCC 4 cut(s) 406, 792, 894, 1310
BtgI CCRYGG 1 cut(s) 804
BtsCI GGATG 5 cut(s) 145, 219, 610, 616, 1321
BtsI GCAGTG 1 cut(s) 1141
BtsIMutI CAGTG 3 cut(s) 1141, 1225, 1467
BveI ACCTGC 2 cut(s) 58, 490
Cac8I GCNNGC 3 cut(s) 518, 1264, 1363
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 7 cut(s) 173, 404, 593, 626, 705, 1055, 1397
CseI GACGC 1 cut(s) 1301
Csp6I GTAC 1 cut(s) 1023
CviAII CATG 3 cut(s) 67, 517, 805
CviQI GTAC 1 cut(s) 1023
DdeI CTNAG 7 cut(s) 25, 438, 540, 762, 1080, 1087, 1116
DpnI GATC 5 cut(s) 30, 784, 826, 844, 996
DpnII GATC 5 cut(s) 28, 782, 824, 842, 994
DriI GACNNNNNGTC 1 cut(s) 1548
EaeI YGGCCR 2 cut(s) 790, 892
Eam1104I CTCTTC 1 cut(s) 1409
Eam1105I GACNNNNNGTC 1 cut(s) 1548
EarI CTCTTC 1 cut(s) 1409
EciI GGCGGA 1 cut(s) 1493
Ecl136II GAGCTC 2 cut(s) 550, 1365
Eco130I CCWWGG 5 cut(s) 652, 804, 946, 1058, 1311
Eco24I GRGCYC 2 cut(s) 552, 1367
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 6 cut(s) 173, 593, 626, 705, 1055, 1397
Eco53kI GAGCTC 2 cut(s) 550, 1365
Eco57I CTGAAG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 545
EcoICRI GAGCTC 2 cut(s) 550, 1365
EcoRII CCWGG 2 cut(s) 123, 894
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 5 cut(s) 652, 804, 946, 1058, 1311
EcoT22I ATGCAT 1 cut(s) 879
EcoT38I GRGCYC 2 cut(s) 552, 1367
ErhI CCWWGG 5 cut(s) 652, 804, 946, 1058, 1311
FaeI CATG 3 cut(s) 70, 520, 808
FaqI GGGAC 4 cut(s) 68, 296, 411, 456
FatI CATG 3 cut(s) 66, 516, 804
FauNDI CATATG 3 cut(s) 400, 778, 787
FblI GTMKAC 2 cut(s) 363, 916
Fnu4HI GCNGC 5 cut(s) 217, 420, 671, 1107, 1296
FokI GGATG 5 cut(s) 132, 206, 597, 603, 1328
FriOI GRGCYC 2 cut(s) 552, 1367
Fsp4HI GCNGC 5 cut(s) 217, 420, 671, 1107, 1296
FspBI CTAG 4 cut(s) 54, 213, 513, 1263
GluI GCNGC 5 cut(s) 217, 420, 671, 1107, 1296
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 4 cut(s) 406, 792, 894, 1310
HapII CCGG 4 cut(s) 86, 189, 201, 749
HgaI GACGC 1 cut(s) 1301
Hin1II CATG 3 cut(s) 70, 520, 808
HincII GTYRAC 2 cut(s) 571, 964
HindII GTYRAC 2 cut(s) 571, 964
HindIII AAGCTT 2 cut(s) 1286, 1591
HinfI GANTC 5 cut(s) 10, 1078, 1100, 1112, 1154
HpaII CCGG 4 cut(s) 86, 189, 201, 749
HphI GGTGA 5 cut(s) 203, 477, 544, 593, 1168
Hpy166II GTNNAC 7 cut(s) 364, 571, 917, 964, 1234, 1334, 1451
Hpy188I TCNGA 6 cut(s) 28, 40, 439, 593, 700, 718
Hpy188III TCNNGA 4 cut(s) 67, 662, 1304, 1553
Hpy8I GTNNAC 7 cut(s) 364, 571, 917, 964, 1234, 1334, 1451
HpyAV CCTTC 5 cut(s) 64, 305, 444, 723, 978
HpyCH4V TGCA 5 cut(s) 419, 520, 724, 877, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 403, 1503, 1581, 1590
HpyF3I CTNAG 7 cut(s) 25, 438, 540, 762, 1080, 1087, 1116
Hsp92II CATG 3 cut(s) 70, 520, 808
Kzo9I GATC 5 cut(s) 28, 782, 824, 842, 994
LguI GCTCTTC 1 cut(s) 1409
LmnI GCTCC 6 cut(s) 43, 73, 136, 166, 1093, 1144
Lsp1109I GCAGC 5 cut(s) 228, 431, 682, 1093, 1282
LweI GCATC 3 cut(s) 228, 491, 1251
MaeI CTAG 4 cut(s) 54, 213, 513, 1263
MaeIII GTNAC 3 cut(s) 810, 1174, 1540
MalI GATC 5 cut(s) 30, 784, 826, 844, 996
MboI GATC 5 cut(s) 28, 782, 824, 842, 994
MboII GAAGA 5 cut(s) 726, 729, 1235, 1334, 1426
MflI RGATCY 2 cut(s) 28, 842
MhlI GDGCHC 3 cut(s) 552, 1149, 1367
MlsI TGGCCA 2 cut(s) 792, 894
MluCI AATT 3 cut(s) 921, 1370, 1576
MluNI TGGCCA 2 cut(s) 792, 894
MlyI GAGTC 2 cut(s) 4, 1072
MmeI TCCRAC 4 cut(s) 560, 678, 1182, 1375
Mox20I TGGCCA 2 cut(s) 792, 894
Mph1103I ATGCAT 1 cut(s) 879
MscI TGGCCA 2 cut(s) 792, 894
MseI TTAA 3 cut(s) 114, 633, 1619
MslI CAYNNNNRTG 2 cut(s) 803, 882
Msp20I TGGCCA 2 cut(s) 792, 894
MspI CCGG 4 cut(s) 86, 189, 201, 749
MspR9I CCNGG 3 cut(s) 86, 125, 896
Mva1269I GAATGC 2 cut(s) 355, 730
MvaI CCWGG 2 cut(s) 125, 896
MwoI GCNNNNNNNGC 5 cut(s) 158, 403, 1503, 1581, 1590
NciI CCSGG 1 cut(s) 86
NcoI CCATGG 1 cut(s) 804
NdeI CATATG 3 cut(s) 400, 778, 787
NdeII GATC 5 cut(s) 28, 782, 824, 842, 994
NheI GCTAGC 1 cut(s) 1262
NlaIII CATG 3 cut(s) 70, 520, 808
NmeAIII GCCGAG 1 cut(s) 750
NmuCI GTSAC 3 cut(s) 810, 1174, 1540
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 1 cut(s) 851
PaeI GCATGC 1 cut(s) 520
PagI TCATGA 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 1409
PcsI WCGNNNNNNNCGW 1 cut(s) 1358
PctI GAATGC 2 cut(s) 355, 730
PfeI GAWTC 3 cut(s) 1100, 1112, 1154
PflFI GACNNNGTC 1 cut(s) 1172
PflMI CCANNNNNTGG 2 cut(s) 170, 653
PkrI GCNGC 5 cut(s) 218, 421, 672, 1108, 1297
PleI GAGTC 2 cut(s) 4, 1072
PpsI GAGTC 2 cut(s) 4, 1072
PshAI GACNNNNGTC 2 cut(s) 969, 1082
PsiI TTATAA 2 cut(s) 861, 1017
Psp124BI GAGCTC 2 cut(s) 552, 1367
Psp6I CCWGG 2 cut(s) 123, 894
PspGI CCWGG 2 cut(s) 123, 894
PspPI GGNCC 7 cut(s) 173, 404, 593, 626, 705, 1055, 1397
PstI CTGCAG 1 cut(s) 421
PsuI RGATCY 2 cut(s) 28, 842
PsyI GACNNNGTC 1 cut(s) 1172
RsaI GTAC 1 cut(s) 1024
RsaNI GTAC 1 cut(s) 1023
RseI CAYNNNNRTG 2 cut(s) 803, 882
SacI GAGCTC 2 cut(s) 552, 1367
SapI GCTCTTC 1 cut(s) 1409
SaqAI TTAA 3 cut(s) 114, 633, 1619
SatI GCNGC 5 cut(s) 217, 420, 671, 1107, 1296
Sau3AI GATC 5 cut(s) 28, 782, 824, 842, 994
Sau96I GGNCC 7 cut(s) 173, 404, 593, 626, 705, 1055, 1397
SchI GAGTC 2 cut(s) 4, 1072
ScrFI CCNGG 3 cut(s) 86, 125, 896
SduI GDGCHC 3 cut(s) 552, 1149, 1367
SfaNI GCATC 3 cut(s) 228, 491, 1251
SfcI CTRYAG 3 cut(s) 250, 417, 1648
SfuI TTCGAA 1 cut(s) 851
SinI GGWCC 6 cut(s) 173, 593, 626, 705, 1055, 1397
SmiMI CAYNNNNRTG 2 cut(s) 803, 882
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 520
Sse9I AATT 3 cut(s) 921, 1370, 1576
SsiI CCGC 1 cut(s) 1504
SspMI CTAG 4 cut(s) 54, 213, 513, 1263
SstI GAGCTC 2 cut(s) 552, 1367
StyD4I CCNGG 3 cut(s) 84, 123, 894
StyI CCWWGG 5 cut(s) 652, 804, 946, 1058, 1311
TaqI TCGA 4 cut(s) 851, 973, 1051, 1552
TasI AATT 3 cut(s) 921, 1370, 1576
TfiI GAWTC 3 cut(s) 1100, 1112, 1154
Tru1I TTAA 3 cut(s) 114, 633, 1619
Tru9I TTAA 3 cut(s) 114, 633, 1619
TscAI CASTG 3 cut(s) 1141, 1225, 1467
TseFI GTSAC 3 cut(s) 810, 1174, 1540
TseI GCWGC 5 cut(s) 216, 419, 670, 1106, 1295
Tsp45I GTSAC 3 cut(s) 810, 1174, 1540
TspDTI ATGAA 4 cut(s) 83, 134, 276, 1192
TspGWI ACGGA 1 cut(s) 459
TspRI CASTG 3 cut(s) 1141, 1225, 1467
Tth111I GACNNNGTC 1 cut(s) 1172
Van91I CCANNNNNTGG 2 cut(s) 170, 653
VpaK11BI GGWCC 6 cut(s) 173, 593, 626, 705, 1055, 1397
XceI RCATGY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 3 cut(s) 374, 650, 953
XmiI GTMKAC 2 cut(s) 363, 916
XspI CTAG 4 cut(s) 54, 213, 513, 1263
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.