Rorug02G0407900

YT521-B-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001677
Physical Location & Seq
Reverse (-)
52002149 .. 52005243
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0407900.1

Sequence Viewer

Length: 942 bp
ATGCCTGCGCAGAAGGTTGAGACTGGTCACCAGGACACTGTCCACGATGTCGTCATGGATTACTATGGCAAGCGCCTAGCCACAGCTTCGTCGGACAACACCATTAAGATAGTGGGAGTGAGCAACTCGGCCTCGCAACATCTTGCGACTTTGACTGGTCACCAGGGACCTGTTTGGCAGGTTGCTTGGGCTCACCCGAAATTCGGGTCTTTACTTGCTTCTTGTTCTTATGATGGGCGTGTCATTCTGTGGAAGGAAGGCAATCAGAACGAGTGGACACAAGCCCATGTTTTTGATGACCACAAATCGTCTGTCAACTCAATTGCTTGGGCTCCTCATGAACTCGGTCTCTGTTTGGCTTGTGGTTCTTCCGATGGGAATATCTCGGTTTTCACTGCTAAAGCAGACAGTGGTTGGGAGACCACAAAGATCGACCAAGCTCACCCAGTTGGTGTAACCTCTGTTTCCTGGGCTCCCTCAACAGCTCCTGGTGCTCTTGTTGGCTCTGGTCTGCTTGACACTGTTCAGAAGCTGTGTTCTGGTGGTTGTGATAACACTGTAAAGGTCTGGAAGCTTACTAATGGCGTTTGGAAAATGGACTGTTTTCCAGCTCTTCAAATGCACGTTGATTGGGTTAGGGATGTTGCTTGGGCACCTAACTTGGGACTACCAAAATCTACTATTGCCAGTGCATCACAGGATGGGAAAGTTATTATCTGGACTGTGGCCAAGGATGGGGATCAATGGGAAGGTAAGGTGCTGCATGATTTCAAGGCACCTGTTTGGAGGGTCTCATGGTCACTTACCGGGAACATATTGGCCGTGGCTGATGGGAACAACAGCGTGACATTGTGGAAGGAAGAAGTTGATGGGGAGTGGCAACAGATACCTAATGCGGAATATGGACTTCAAGAACAGCCGAGAGCTCATATAATGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YTH

Protein Analysis

313

Amino Acids

34.08

Weight (kDa)

5.69

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 4 - 159 2.6e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 8 - 159 5.1e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 131 3.3e-14 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 9 - 137 2.9e-06 MABP1/WDR62 second WD40 domain
WDR55 PF24796 9 - 134 8.7e-06 WDR55
WD40_CDC20-Fz PF24807 9 - 160 4.3e-14 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 9 - 109 9.9e-12 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 10 - 91 2e-14 THOC3 beta-propeller domain
WD40_Prp19 PF24814 23 - 171 3.5e-13 Prp19 WD40 domain
WD40_Gbeta PF25391 25 - 160 2.7e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR35_TULP_N PF24797 43 - 160 9.9e-08 WDR35/TULP4 N-terminal
WD40 PF00400 46 - 84 1.6e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 49 - 135 6.9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2 PF23414 69 - 158 9.5e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 91 - 286 2.6e-11 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 173 - 246 8.3e-07 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 176 - 287 9.6e-07 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 176 - 288 2e-11 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 176 - 286 5.4e-13 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 9
Acc36I ACCTGC 1 cut(s) 169
AccB1I GGYRCC 2 cut(s) 652, 775
AciI CCGC 1 cut(s) 896
AclWI GGATC 1 cut(s) 747
AcoI YGGCCR 2 cut(s) 726, 819
AcsI RAATTY 1 cut(s) 200
AfiI CCNNNNNNNGG 3 cut(s) 203, 662, 735
AgsI TTSAA 3 cut(s) 617, 772, 911
AjnI CCWGG 4 cut(s) 30, 162, 467, 487
AluBI AGCT 7 cut(s) 86, 440, 485, 532, 574, 611, 926
AluI AGCT 7 cut(s) 86, 440, 485, 532, 574, 611, 926
Alw21I GWGCWC 2 cut(s) 496, 928
Alw26I GTCTC 4 cut(s) 14, 353, 413, 796
AlwI GGATC 1 cut(s) 747
AlwNI CAGNNNCTG 2 cut(s) 488, 532
AoxI GGCC 3 cut(s) 129, 726, 819
ApeKI GCWGC 1 cut(s) 760
ApoI RAATTY 1 cut(s) 200
ArsI GACNNNNNNTTYG 4 cut(s) 191, 223, 297, 329
AspLEI GCGC 2 cut(s) 10, 75
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 808
AsuHPI GGTGA 4 cut(s) 20, 152, 185, 434
AvaII GGWCC 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 655
BalI TGGCCA 1 cut(s) 728
BanI GGYRCC 2 cut(s) 652, 775
BanII GRGCYC 4 cut(s) 193, 334, 475, 928
Bbv12I GWGCWC 2 cut(s) 496, 928
BbvI GCAGC 1 cut(s) 747
BccI CCATC 6 cut(s) 227, 368, 695, 728, 824, 863
BceAI ACGGC 1 cut(s) 806
BciT130I CCWGG 4 cut(s) 32, 164, 469, 489
BcnI CCSGG 1 cut(s) 808
BcoDI GTCTC 4 cut(s) 14, 353, 413, 796
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 76
BfuAI ACCTGC 1 cut(s) 169
BisI GCNGC 1 cut(s) 761
BlsI GCNGC 1 cut(s) 762
Bme1390I CCNGG 5 cut(s) 32, 164, 469, 489, 808
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 5 cut(s) 168, 333, 474, 654, 777
BmrFI CCNGG 5 cut(s) 32, 164, 469, 489, 808
BmrI ACTGGG 1 cut(s) 440
BmsI GCATC 1 cut(s) 701
BmuI ACTGGG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 808
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 3 cut(s) 353, 413, 796
BsaJI CCNNGG 4 cut(s) 163, 468, 729, 822
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 662, 735
Bse1I ACTGG 4 cut(s) 28, 160, 446, 687
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 4 cut(s) 32, 164, 469, 489
BseDI CCNNGG 4 cut(s) 163, 468, 729, 822
BseGI GGATG 3 cut(s) 646, 706, 739
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 3 cut(s) 203, 662, 735
BseNI ACTGG 4 cut(s) 28, 160, 446, 687
BseRI GAGGAG 1 cut(s) 324
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 1 cut(s) 747
BshFI GGCC 3 cut(s) 131, 728, 821
BshNI GGYRCC 2 cut(s) 652, 775
BsiHKAI GWGCWC 2 cut(s) 496, 928
BsiSI CCGG 1 cut(s) 807
BslFI GGGAC 2 cut(s) 180, 678
BslI CCNNNNNNNGG 3 cut(s) 203, 662, 735
BsmAI GTCTC 4 cut(s) 14, 353, 413, 796
BsmFI GGGAC 2 cut(s) 180, 678
BsnI GGCC 3 cut(s) 131, 728, 821
Bso31I GGTCTC 3 cut(s) 353, 413, 796
Bsp1286I GDGCHC 6 cut(s) 193, 334, 475, 496, 655, 928
Bsp143I GATC 2 cut(s) 429, 739
BspACI CCGC 1 cut(s) 896
BspANI GGCC 3 cut(s) 131, 728, 821
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 5 cut(s) 168, 333, 474, 654, 777
BspMI ACCTGC 1 cut(s) 169
BspPI GGATC 1 cut(s) 747
BspQI GCTCTTC 1 cut(s) 618
BspT107I GGYRCC 2 cut(s) 652, 775
BspTNI GGTCTC 3 cut(s) 353, 413, 796
BsrI ACTGG 4 cut(s) 28, 160, 446, 687
BssECI CCNNGG 4 cut(s) 163, 468, 729, 822
BssMI GATC 2 cut(s) 429, 739
BssT1I CCWWGG 1 cut(s) 729
Bst2UI CCWGG 4 cut(s) 32, 164, 469, 489
Bst4CI ACNGT 6 cut(s) 40, 410, 523, 559, 602, 724
Bst6I CTCTTC 1 cut(s) 618
BstC8I GCNNGC 2 cut(s) 6, 71
BstDSI CCRYGG 1 cut(s) 822
BstEII GGTNACC 2 cut(s) 26, 158
BstF5I GGATG 3 cut(s) 646, 706, 739
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 10, 75
BstKTI GATC 2 cut(s) 432, 742
BstMAI GTCTC 4 cut(s) 14, 353, 413, 796
BstMBI GATC 2 cut(s) 429, 739
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstNI CCWGG 4 cut(s) 32, 164, 469, 489
BstPI GGTNACC 2 cut(s) 26, 158
BstSCI CCNGG 5 cut(s) 30, 162, 467, 487, 806
BstSLI GKGCMC 1 cut(s) 655
BstV1I GCAGC 1 cut(s) 747
BsuRI GGCC 3 cut(s) 131, 728, 821
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 3 cut(s) 646, 706, 739
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 6 cut(s) 36, 393, 415, 519, 555, 694
BveI ACCTGC 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 6, 71
CaiI CAGNNNCTG 2 cut(s) 488, 532
CciI TCATGA 1 cut(s) 337
CfoI GCGC 2 cut(s) 10, 75
Cfr13I GGNCC 1 cut(s) 167
CviAII CATG 5 cut(s) 55, 287, 338, 764, 795
DpnI GATC 2 cut(s) 431, 741
DpnII GATC 2 cut(s) 429, 739
EaeI YGGCCR 2 cut(s) 726, 819
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Ecl136II GAGCTC 1 cut(s) 926
Eco130I CCWWGG 1 cut(s) 729
Eco24I GRGCYC 4 cut(s) 193, 334, 475, 928
Eco31I GGTCTC 3 cut(s) 353, 413, 796
Eco47I GGWCC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 926
Eco91I GGTNACC 2 cut(s) 26, 158
EcoICRI GAGCTC 1 cut(s) 926
EcoO109I RGGNCCY 1 cut(s) 167
EcoO65I GGTNACC 2 cut(s) 26, 158
EcoRII CCWGG 4 cut(s) 30, 162, 467, 487
EcoT14I CCWWGG 1 cut(s) 729
EcoT38I GRGCYC 4 cut(s) 193, 334, 475, 928
ErhI CCWWGG 1 cut(s) 729
FaeI CATG 5 cut(s) 58, 290, 341, 767, 798
FaqI GGGAC 2 cut(s) 180, 678
FatI CATG 5 cut(s) 54, 286, 337, 763, 794
Fnu4HI GCNGC 1 cut(s) 761
FokI GGATG 3 cut(s) 653, 713, 746
FriOI GRGCYC 4 cut(s) 193, 334, 475, 928
Fsp4HI GCNGC 1 cut(s) 761
FspBI CTAG 1 cut(s) 77
FspI TGCGCA 1 cut(s) 9
GlaI GCGC 2 cut(s) 9, 74
GluI GCNGC 1 cut(s) 761
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 3 cut(s) 131, 728, 821
HapII CCGG 1 cut(s) 807
HhaI GCGC 2 cut(s) 10, 75
Hin1II CATG 5 cut(s) 58, 290, 341, 767, 798
Hin6I GCGC 2 cut(s) 8, 73
HinP1I GCGC 2 cut(s) 8, 73
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 572
HpaII CCGG 1 cut(s) 807
HphI GGTGA 4 cut(s) 20, 152, 185, 434
Hpy166II GTNNAC 3 cut(s) 43, 276, 316
Hpy188I TCNGA 4 cut(s) 94, 267, 373, 528
Hpy188III TCNNGA 4 cut(s) 338, 568, 718, 911
Hpy8I GTNNAC 3 cut(s) 43, 276, 316
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 5 cut(s) 7, 247, 251, 743, 850
HpyCH4III ACNGT 6 cut(s) 40, 410, 523, 559, 602, 724
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 3 cut(s) 622, 692, 763
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 5 cut(s) 58, 290, 341, 767, 798
HspAI GCGC 2 cut(s) 8, 73
Kzo9I GATC 2 cut(s) 429, 739
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 3 cut(s) 337, 478, 490
Lsp1109I GCAGC 1 cut(s) 747
LweI GCATC 1 cut(s) 701
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 5 cut(s) 26, 158, 454, 798, 844
MalI GATC 2 cut(s) 431, 741
MboI GATC 2 cut(s) 429, 739
MboII GAAGA 3 cut(s) 360, 605, 872
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 6 cut(s) 193, 334, 475, 496, 655, 928
MlsI TGGCCA 1 cut(s) 728
MluCI AATT 2 cut(s) 200, 321
MluNI TGGCCA 1 cut(s) 728
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 5 cut(s) 142, 345, 469, 487, 780
Mox20I TGGCCA 1 cut(s) 728
MscI TGGCCA 1 cut(s) 728
MseI TTAA 1 cut(s) 105
Msp20I TGGCCA 1 cut(s) 728
MspI CCGG 1 cut(s) 807
MspR9I CCNGG 5 cut(s) 32, 164, 469, 489, 808
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 4 cut(s) 32, 164, 469, 489
MwoI GCNNNNNNNGC 1 cut(s) 491
NciI CCSGG 1 cut(s) 808
NdeII GATC 2 cut(s) 429, 739
NlaIII CATG 5 cut(s) 58, 290, 341, 767, 798
NlaIV GGNNCC 5 cut(s) 168, 333, 474, 654, 777
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 4 cut(s) 26, 158, 798, 844
NsbI TGCGCA 1 cut(s) 9
PagI TCATGA 1 cut(s) 337
PciSI GCTCTTC 1 cut(s) 618
PflFI GACNNNGTC 1 cut(s) 38
PkrI GCNGC 1 cut(s) 762
PpuMI RGGWCCY 1 cut(s) 167
Psp124BI GAGCTC 1 cut(s) 928
Psp5II RGGWCCY 1 cut(s) 167
Psp6I CCWGG 4 cut(s) 30, 162, 467, 487
PspEI GGTNACC 2 cut(s) 26, 158
PspGI CCWGG 4 cut(s) 30, 162, 467, 487
PspN4I GGNNCC 5 cut(s) 168, 333, 474, 654, 777
PspPI GGNCC 1 cut(s) 167
PspPPI RGGWCCY 1 cut(s) 167
PstNI CAGNNNCTG 2 cut(s) 488, 532
PsyI GACNNNGTC 1 cut(s) 38
SacI GAGCTC 1 cut(s) 928
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 1 cut(s) 761
Sau3AI GATC 2 cut(s) 429, 739
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 5 cut(s) 32, 164, 469, 489, 808
SduI GDGCHC 6 cut(s) 193, 334, 475, 496, 655, 928
SfaNI GCATC 1 cut(s) 701
SinI GGWCC 1 cut(s) 167
Sse9I AATT 2 cut(s) 200, 321
SsiI CCGC 1 cut(s) 896
SspMI CTAG 1 cut(s) 77
SstI GAGCTC 1 cut(s) 928
StyD4I CCNGG 5 cut(s) 30, 162, 467, 487, 806
StyI CCWWGG 1 cut(s) 729
TaaI ACNGT 6 cut(s) 40, 410, 523, 559, 602, 724
TaiI ACGT 1 cut(s) 627
TaqI TCGA 1 cut(s) 432
TaqII GACCGA 1 cut(s) 335
TasI AATT 2 cut(s) 200, 321
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 6 cut(s) 43, 400, 415, 526, 562, 694
TseFI GTSAC 4 cut(s) 26, 158, 798, 844
TseI GCWGC 1 cut(s) 760
Tsp45I GTSAC 4 cut(s) 26, 158, 798, 844
TspDTI ATGAA 1 cut(s) 354
TspRI CASTG 6 cut(s) 43, 400, 415, 526, 562, 694
Tth111I GACNNNGTC 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.