Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010945

Physical Location & Seq

Genomic Coordinates Forward (+)

5055741 .. 5058249

Gene Structure 2509 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G15020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGTTCCGATCAATGATTGTTCGATCTGCTTCCCCAGTGAAGCAGGGTCTTCTCCGCAGAGGATTCGCCTCTGAATCTGTTCCCGATCGCAAAGTCGTCATCCTCGGTGCCGCCGGTGGGATCGGCCAGCCACTTTCTCTTCTCATGAAGCTTAACCCTCTCGTCTCTTCTCTCTCCCTCTATGATATCGCCAACACTCCCGGCGTTGCTGCTGACGTCGGTCACATCAACACCCGATCTCAGGTTTCTGGGTACATGGGTGATGATGATTTGGGGAAAGCTCTAGAAGGCGCTGACCTCGTCATTATTCCAGCTGGTGTCCCAAGGAAGCCTGGCATGACCCGTGATGATCTTTTCAACATCAATGCTGGCATTGTCAAGAACCTCAGCATCGCCATCGCCAAGTATTGCCCACAAGCACTTGTCAACATGATCAGCAACCCTGTGAACTCCACTGTTCCAATTGCAGCTGAGATTTTTAAGAAGGCTGGTACCTATGATGAGAAGAAATTATTCGGTGTCACCACTCTTGATGTTGTCAGGGCTAGGACTTTCTATGCTGGAAAATCGGATGTCAATGTTGCAGAGGTTAATGTTCCAGTTGTTGGTGGTCATGCTGGCATCACGATTCTTCCTCTCTTTTCTCAGGCTAGCCCTCAAGCCAACTTGTCGGATGATTTAATCAGGGCCCTCACAAAGCGTACCCAGGACGGAGGGACAGAAGTCGTGGAGGCAAAAGCTGGAAAGGGTTCAGCTACATTGTCAATGGCCTATGCGGGAGCACTCTTTGCTGATGCATGCTTAAAGGGACTCAACGGTGTTCCAAATGTGGTGGAATGCTCATTCGTCCAATCTACCATCACCGAGCTTCCTTTCTTCGCCTCAAAGGTAAGACTGGGAAAGAACGGAGTGGAGGAAGTGCTAGATCTAGGGCCACTCTCAGACTTTGAAAAGGAAGGCTTAGAAGCCCTCAAGGCAGAACTCAAATCCTCTATCGAGAAGGGCATCAAATTTGCCAACCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0016020 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0030060 GO:0030312 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

341

Amino Acids

35.87

Weight (kDa)

8.3

Isoelectric Point (pI)

39.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	31 - 173	4.1e-46	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	175 - 338	9.6e-50	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53240 AT3G15020 AT3G15020
fragaria_vesca	FvH4_3g18670
malus_domestica	MD03G1240100.v1.1 MD11G1259400.v1.1
prunus_persica	Prupe.4G058400_v2.0.a1 Prupe.4G170500_v2.0.a1
pyrus_communis	pycom03g18530 pycom11g22920
rosa_chinensis	RchiOBHm_Chr5g0031491
rosa_laevigata	RLG00000033315
rosa_multiflora	Rmu_sc0006344.1_g000010
rosa_roxburghii	Rroxscaffold_1G00048830
rosa_rugosa	Rorug05G0126300
rosa_samantha	Rh5AG219200 Rh5BG218600 Rh5CG242200 Rh5DG224100
rosa_wichuraiana	Rw5G019990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	219
Acc65I	GGTACC	1 cut(s)	491
AccB1I	GGYRCC	2 cut(s)	107, 491
AccB7I	CCANNNNNTGG	1 cut(s)	605
AciI	CCGC	3 cut(s)	55, 111, 776
AclWI	GGATC	1 cut(s)	128
AcoI	YGGCCR	1 cut(s)	124
AcsI	RAATTY	1 cut(s)	1010
AcyI	GRCGYC	1 cut(s)	216
AfaI	GTAC	3 cut(s)	254, 493, 703
AfiI	CCNNNNNNNGG	3 cut(s)	117, 241, 605
AgsI	TTSAA	2 cut(s)	358, 950
AjnI	CCWGG	2 cut(s)	331, 705
AluBI	AGCT	7 cut(s)	151, 281, 314, 470, 740, 755, 868
AluI	AGCT	7 cut(s)	151, 281, 314, 470, 740, 755, 868
Alw21I	GWGCWC	1 cut(s)	784
Alw26I	GTCTC	1 cut(s)	169
AlwI	GGATC	1 cut(s)	128
AoxI	GGCC	4 cut(s)	124, 687, 768, 932
ApaI	GGGCCC	1 cut(s)	691
ApeKI	GCWGC	2 cut(s)	209, 467
ApoI	RAATTY	1 cut(s)	1010
Asp700I	GAANNNNTTC	3 cut(s)	78, 512, 748
Asp718I	GGTACC	1 cut(s)	491
AspLEI	GCGC	1 cut(s)	293
AspS9I	GGNCC	3 cut(s)	687, 688, 932
AsuC2I	CCSGG	1 cut(s)	201
AsuHPI	GGTGA	3 cut(s)	272, 514, 853
AsuNHI	GCTAGC	1 cut(s)	650
BaeGI	GKGCMC	1 cut(s)	691
BaeI	ACNNNNGTAYC	2 cut(s)	244, 277
BanI	GGYRCC	2 cut(s)	107, 491
BanII	GRGCYC	1 cut(s)	691
BbsI	GAAGAC	1 cut(s)	41
Bbv12I	GWGCWC	1 cut(s)	784
BbvCI	CCTCAGC	1 cut(s)	386
BbvI	GCAGC	2 cut(s)	196, 479
BccI	CCATC	2 cut(s)	404, 866
BcgI	CGANNNNNNTGC	4 cut(s)	46, 80, 379, 413
BciT130I	CCWGG	2 cut(s)	333, 707
BclI	TGATCA	1 cut(s)	432
BcnI	CCSGG	1 cut(s)	201
BcoDI	GTCTC	1 cut(s)	169
BfaI	CTAG	5 cut(s)	284, 546, 651, 923, 929
BfoI	RGCGCY	1 cut(s)	294
BglI	GCCNNNNNGGC	1 cut(s)	974
BglII	AGATCT	1 cut(s)	925
BisI	GCNGC	3 cut(s)	111, 210, 468
BlsI	GCNGC	3 cut(s)	112, 211, 469
Bme1390I	CCNGG	3 cut(s)	201, 333, 707
BmgT120I	GGNCC	3 cut(s)	687, 688, 932
BmiI	GGNNCC	3 cut(s)	109, 493, 689
BmrFI	CCNGG	3 cut(s)	201, 333, 707
BmrI	ACTGGG	2 cut(s)	29, 905
BmsI	GCATC	4 cut(s)	399, 630, 784, 1014
BmtI	GCTAGC	1 cut(s)	654
BmuI	ACTGGG	2 cut(s)	29, 905
BoxI	GACNNNNGTC	2 cut(s)	219, 722
BpiI	GAAGAC	1 cut(s)	41
Bpu10I	CCTNAGC	1 cut(s)	386
BpuEI	CTTGAG	2 cut(s)	642, 956
BpuMI	CCSGG	1 cut(s)	201
BsaHI	GRCGYC	1 cut(s)	216
BsaJI	CCNNGG	3 cut(s)	103, 323, 705
Bsc4I	CCNNNNNNNGG	3 cut(s)	117, 241, 605
Bse118I	RCCGGY	1 cut(s)	113
Bse1I	ACTGG	3 cut(s)	35, 599, 900
BseBI	CCWGG	2 cut(s)	333, 707
BseDI	CCNNGG	3 cut(s)	103, 323, 705
BseGI	GGATG	3 cut(s)	99, 577, 679
BseLI	CCNNNNNNNGG	3 cut(s)	117, 241, 605
BseMII	CTCAG	5 cut(s)	254, 400, 462, 659, 954
BseNI	ACTGG	3 cut(s)	35, 599, 900
BseSI	GKGCMC	1 cut(s)	691
BseXI	GCAGC	2 cut(s)	196, 479
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	4 cut(s)	126, 689, 770, 934
BshNI	GGYRCC	2 cut(s)	107, 491
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	1 cut(s)	784
BsiSI	CCGG	2 cut(s)	114, 201
BslFI	GGGAC	3 cut(s)	305, 730, 822
BslI	CCNNNNNNNGG	3 cut(s)	117, 241, 605
BsmAI	GTCTC	1 cut(s)	169
BsmBI	CGTCTC	1 cut(s)	169
BsmFI	GGGAC	3 cut(s)	305, 730, 822
BsmI	GAATGC	1 cut(s)	842
BsnI	GGCC	4 cut(s)	126, 689, 770, 934
Bsp120I	GGGCCC	1 cut(s)	687
Bsp1286I	GDGCHC	2 cut(s)	691, 784
Bsp143I	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
BspACI	CCGC	3 cut(s)	55, 111, 776
BspANI	GGCC	4 cut(s)	126, 689, 770, 934
BspCNI	CTCAG	5 cut(s)	253, 399, 463, 658, 953
BspHI	TCATGA	1 cut(s)	144
BspLI	GGNNCC	3 cut(s)	109, 493, 689
BspOI	GCTAGC	1 cut(s)	654
BspPI	GGATC	1 cut(s)	128
BspT107I	GGYRCC	2 cut(s)	107, 491
BsrFI	RCCGGY	1 cut(s)	113
BsrI	ACTGG	3 cut(s)	35, 599, 900
BssAI	RCCGGY	1 cut(s)	113
BssECI	CCNNGG	3 cut(s)	103, 323, 705
BssMI	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
BssNI	GRCGYC	1 cut(s)	216
BssT1I	CCWWGG	1 cut(s)	323
Bst2UI	CCWGG	2 cut(s)	333, 707
Bst4CI	ACNGT	2 cut(s)	457, 818
Bst6I	CTCTTC	2 cut(s)	144, 172
BstACI	GRCGYC	1 cut(s)	216
BstAPI	GCANNNNNTGC	1 cut(s)	788
BstC8I	GCNNGC	5 cut(s)	128, 370, 619, 652, 799
BstDEI	CTNAG	6 cut(s)	240, 386, 471, 645, 940, 961
BstF5I	GGATG	3 cut(s)	99, 577, 679
BstH2I	RGCGCY	1 cut(s)	294
BstHHI	GCGC	1 cut(s)	293
BstKTI	GATC	8 cut(s)	11, 26, 88, 123, 239, 352, 435, 928
BstMAI	GTCTC	1 cut(s)	169
BstMBI	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
BstMCI	CGRYCG	1 cut(s)	88
BstMWI	GCNNNNNNNGC	2 cut(s)	788, 974
BstNI	CCWGG	2 cut(s)	333, 707
BstNSI	RCATGY	1 cut(s)	801
BstPAI	GACNNNNGTC	2 cut(s)	219, 722
BstSCI	CCNGG	3 cut(s)	199, 331, 705
BstSLI	GKGCMC	1 cut(s)	691
BstV1I	GCAGC	2 cut(s)	196, 479
BstV2I	GAAGAC	1 cut(s)	41
BstX2I	RGATCY	1 cut(s)	925
BstYI	RGATCY	1 cut(s)	925
BsuRI	GGCC	4 cut(s)	126, 689, 770, 934
BtgZI	GCGATG	2 cut(s)	376, 382
BtsCI	GGATG	3 cut(s)	99, 577, 679
BtsIMutI	CAGTG	2 cut(s)	42, 453
Cac8I	GCNNGC	5 cut(s)	128, 370, 619, 652, 799
CciI	TCATGA	1 cut(s)	144
CfoI	GCGC	1 cut(s)	293
Cfr10I	RCCGGY	1 cut(s)	113
Cfr13I	GGNCC	3 cut(s)	687, 688, 932
Csp6I	GTAC	3 cut(s)	253, 492, 702
CspCI	CAANNNNNGTGG	2 cut(s)	813, 848
CviAII	CATG	6 cut(s)	145, 256, 337, 430, 614, 798
CviQI	GTAC	3 cut(s)	253, 492, 702
DdeI	CTNAG	6 cut(s)	240, 386, 471, 645, 940, 961
DpnI	GATC	8 cut(s)	10, 25, 87, 122, 238, 351, 434, 927
DpnII	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
EaeI	YGGCCR	1 cut(s)	124
Eam1104I	CTCTTC	2 cut(s)	144, 172
EarI	CTCTTC	2 cut(s)	144, 172
Eco130I	CCWWGG	1 cut(s)	323
Eco24I	GRGCYC	1 cut(s)	691
Eco32I	GATATC	1 cut(s)	187
EcoO109I	RGGNCCY	2 cut(s)	687, 688
EcoRII	CCWGG	2 cut(s)	331, 705
EcoRV	GATATC	1 cut(s)	187
EcoT14I	CCWWGG	1 cut(s)	323
EcoT22I	ATGCAT	1 cut(s)	799
EcoT38I	GRGCYC	1 cut(s)	691
ErhI	CCWWGG	1 cut(s)	323
Esp3I	CGTCTC	1 cut(s)	169
FaeI	CATG	6 cut(s)	148, 259, 340, 433, 617, 801
FalI	AAGNNNNNCTT	2 cut(s)	944, 976
FaqI	GGGAC	3 cut(s)	305, 730, 822
FatI	CATG	6 cut(s)	144, 255, 336, 429, 613, 797
FauI	CCCGC	1 cut(s)	769
FbaI	TGATCA	1 cut(s)	432
Fnu4HI	GCNGC	3 cut(s)	111, 210, 468
FokI	GGATG	3 cut(s)	86, 584, 686
FriOI	GRGCYC	1 cut(s)	691
Fsp4HI	GCNGC	3 cut(s)	111, 210, 468
FspBI	CTAG	5 cut(s)	284, 546, 651, 923, 929
GlaI	GCGC	1 cut(s)	292
GluI	GCNGC	3 cut(s)	111, 210, 468
HaeII	RGCGCY	1 cut(s)	294
HaeIII	GGCC	4 cut(s)	126, 689, 770, 934
HapII	CCGG	2 cut(s)	114, 201
HhaI	GCGC	1 cut(s)	293
Hin1I	GRCGYC	1 cut(s)	216
Hin1II	CATG	6 cut(s)	148, 259, 340, 433, 617, 801
Hin6I	GCGC	1 cut(s)	291
HinP1I	GCGC	1 cut(s)	291
HincII	GTYRAC	1 cut(s)	427
HindII	GTYRAC	1 cut(s)	427
HindIII	AAGCTT	1 cut(s)	149
HinfI	GANTC	4 cut(s)	63, 74, 628, 810
HpaII	CCGG	2 cut(s)	114, 201
HphI	GGTGA	3 cut(s)	272, 514, 853
Hpy166II	GTNNAC	2 cut(s)	427, 448
Hpy188I	TCNGA	5 cut(s)	8, 73, 571, 673, 943
Hpy188III	TCNNGA	7 cut(s)	83, 145, 284, 379, 530, 625, 997
Hpy8I	GTNNAC	2 cut(s)	427, 448
Hpy99I	CGWCG	1 cut(s)	221
HpyAV	CCTTC	4 cut(s)	281, 478, 950, 994
HpyCH4III	ACNGT	2 cut(s)	457, 818
HpyCH4IV	ACGT	1 cut(s)	216
HpyCH4V	TGCA	3 cut(s)	467, 584, 797
HpyF10VI	GCNNNNNNNGC	2 cut(s)	788, 974
HpyF3I	CTNAG	6 cut(s)	240, 386, 471, 645, 940, 961
HpySE526I	ACGT	1 cut(s)	216
Hsp92I	GRCGYC	1 cut(s)	216
Hsp92II	CATG	6 cut(s)	148, 259, 340, 433, 617, 801
HspAI	GCGC	1 cut(s)	291
KpnI	GGTACC	1 cut(s)	495
Ksp22I	TGATCA	1 cut(s)	432
Kzo9I	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
LmnI	GCTCC	1 cut(s)	779
Lsp1109I	GCAGC	2 cut(s)	196, 479
LweI	GCATC	4 cut(s)	399, 630, 784, 1014
MaeI	CTAG	5 cut(s)	284, 546, 651, 923, 929
MaeII	ACGT	1 cut(s)	216
MaeIII	GTNAC	2 cut(s)	221, 520
MalI	GATC	8 cut(s)	10, 25, 87, 122, 238, 351, 434, 927
MboI	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
MboII	GAAGA	6 cut(s)	41, 131, 159, 517, 623, 868
MfeI	CAATTG	1 cut(s)	462
MflI	RGATCY	1 cut(s)	925
MhlI	GDGCHC	2 cut(s)	691, 784
MluCI	AATT	3 cut(s)	462, 509, 1010
MlyI	GAGTC	1 cut(s)	804
MmeI	TCCRAC	1 cut(s)	651
Mph1103I	ATGCAT	1 cut(s)	799
MroXI	GAANNNNTTC	3 cut(s)	78, 512, 748
MseI	TTAA	5 cut(s)	153, 480, 591, 680, 803
MspA1I	CMGCKG	2 cut(s)	314, 470
MspI	CCGG	2 cut(s)	114, 201
MspR9I	CCNGG	3 cut(s)	201, 333, 707
MunI	CAATTG	1 cut(s)	462
Mva1269I	GAATGC	1 cut(s)	842
MvaI	CCWGG	2 cut(s)	333, 707
MwoI	GCNNNNNNNGC	2 cut(s)	788, 974
NciI	CCSGG	1 cut(s)	201
NdeII	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
NheI	GCTAGC	1 cut(s)	650
NlaIII	CATG	6 cut(s)	148, 259, 340, 433, 617, 801
NlaIV	GGNNCC	3 cut(s)	109, 493, 689
NmuCI	GTSAC	2 cut(s)	221, 520
NsiI	ATGCAT	1 cut(s)	799
NspI	RCATGY	1 cut(s)	801
PaeI	GCATGC	1 cut(s)	801
PagI	TCATGA	1 cut(s)	144
PctI	GAATGC	1 cut(s)	842
PdmI	GAANNNNTTC	3 cut(s)	78, 512, 748
PfeI	GAWTC	3 cut(s)	63, 74, 628
PflFI	GACNNNGTC	1 cut(s)	299
PflMI	CCANNNNNTGG	1 cut(s)	605
PkrI	GCNGC	3 cut(s)	112, 211, 469
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	1 cut(s)	804
PpsI	GAGTC	1 cut(s)	804
PshAI	GACNNNNGTC	2 cut(s)	219, 722
Psp6I	CCWGG	2 cut(s)	331, 705
PspGI	CCWGG	2 cut(s)	331, 705
PspN4I	GGNNCC	3 cut(s)	109, 493, 689
PspOMI	GGGCCC	1 cut(s)	687
PspPI	GGNCC	3 cut(s)	687, 688, 932
PsuI	RGATCY	1 cut(s)	925
PsyI	GACNNNGTC	1 cut(s)	299
PvuI	CGATCG	1 cut(s)	88
PvuII	CAGCTG	2 cut(s)	314, 470
RsaI	GTAC	3 cut(s)	254, 493, 703
RsaNI	GTAC	3 cut(s)	253, 492, 702
SaqAI	TTAA	5 cut(s)	153, 480, 591, 680, 803
SatI	GCNGC	3 cut(s)	111, 210, 468
Sau3AI	GATC	8 cut(s)	8, 23, 85, 120, 236, 349, 432, 925
Sau96I	GGNCC	3 cut(s)	687, 688, 932
SchI	GAGTC	1 cut(s)	804
ScrFI	CCNGG	3 cut(s)	201, 333, 707
SduI	GDGCHC	2 cut(s)	691, 784
SfaNI	GCATC	4 cut(s)	399, 630, 784, 1014
SgrAI	CRCCGGYG	1 cut(s)	113
SmlI	CTYRAG	2 cut(s)	657, 971
SmoI	CTYRAG	2 cut(s)	657, 971
SphI	GCATGC	1 cut(s)	801
Sse9I	AATT	3 cut(s)	462, 509, 1010
SsiI	CCGC	3 cut(s)	55, 111, 776
SspMI	CTAG	5 cut(s)	284, 546, 651, 923, 929
StyD4I	CCNGG	3 cut(s)	199, 331, 705
StyI	CCWWGG	1 cut(s)	323
TaaI	ACNGT	2 cut(s)	457, 818
TaiI	ACGT	1 cut(s)	219
TaqI	TCGA	2 cut(s)	22, 996
TaqII	GACCGA	1 cut(s)	209
TasI	AATT	3 cut(s)	462, 509, 1010
TauI	GCSGC	1 cut(s)	113
TfiI	GAWTC	3 cut(s)	63, 74, 628
Tru1I	TTAA	5 cut(s)	153, 480, 591, 680, 803
Tru9I	TTAA	5 cut(s)	153, 480, 591, 680, 803
TscAI	CASTG	2 cut(s)	42, 460
TseFI	GTSAC	2 cut(s)	221, 520
TseI	GCWGC	2 cut(s)	209, 467
Tsp45I	GTSAC	2 cut(s)	221, 520
TspDTI	ATGAA	1 cut(s)	161
TspGWI	ACGGA	2 cut(s)	726, 921
TspRI	CASTG	2 cut(s)	42, 460
Tth111I	GACNNNGTC	1 cut(s)	299
Van91I	CCANNNNNTGG	1 cut(s)	605
XapI	RAATTY	1 cut(s)	1010
XbaI	TCTAGA	1 cut(s)	283
XceI	RCATGY	1 cut(s)	801
XmnI	GAANNNNTTC	3 cut(s)	78, 512, 748
XspI	CTAG	5 cut(s)	284, 546, 651, 923, 929
ZraI	GACGTC	1 cut(s)	217
Zsp2I	ATGCAT	1 cut(s)	799

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.