Prupe.4G058400_v2.0.a1

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0010945
Physical Location & Seq
Forward (+)
2787300 .. 2789680
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G058400.1

Sequence Viewer

Length: 1023 bp
ATGAGTGCCACTCTGAGCATGTTGAGGTCTGCTCCCAGAACAGCCGTCCGATCGTACTCATCATCATTTCAGCCGCAGCGCAAAGTCACCATTTTGGGAGCCGCCGGAGGCATAGGCCAGCCATTGTCGCTCCTCATGAAGCTCAACCCTCTCGTCTCCTCCCTCTCCCTGTACGACATAGCCGGCACTCCCGGCGTCGCTGCCGACGTCAGCCACGTCAACACCCTCGCTCAGGTCTCGGGGTTCGCCGGCGAAGAGCAGCTCGGCAAAGCGCTTGAGGGATCTGACCTCGTCATCATCCCGGCCGGCGTTCCCAGAAAGCCCGGAATGACGCGCGATGATCTATTCAACATCAATGCCGGCATCGTCAAGTCTTTGAGCGCTGCCATTGCCAAGTTCTGTCCCAATGCGTTGGTTAATATGATTAGCAATCCGGTGAATTCAACGGTCCCGATTGCGGCGGAGGTGTTCAAGAAGGCAGGGATGTTCGATGAGAAGAAGCTGTTTGGGGTGACAACGCTTGATGTCGTCAGGGCTAAAACTTTCTATGCTGGCAAAGCTAACGTTGAGGCCTCTGAAGTTGATGTTCCTGTAGTTGGTGGTCATGCTGGAATAACCATCCTTCCTCTGTTTTCTCAAGTGACCCTAAAGTCGAAGACTTTGTCGGATGAAGAGATCAAAGCTCTTACCAAGAGAACACAAGATGGAGGGACAGAGGTTGTTGAAGCAAAGGCTGGAAAAGGATCTGCTACTCTATCAATGGCCTATGCCGGGGCAATTTTCGCCGATGCTTGCTTGAAGGGTCTAAACGGGGTTCCAGATGTTGTTGAATGCTCTTTTGTGCAATCAAGTGTTACAGAATTGCCTTTCTTTGCCTCGAAGGTAAGGCTAGGAAAGAATGGCGTGGAGGAAGTTCTTGGATTGGGACCTTTGTCGGACCACGAGAACCAAGGCCTACAAAGTCTAATACCTGAGCTTAAAGCCTCTATAGAGAAGGGAATAAAGTTTGCTAAAGAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
Ldh_1_C Ldh_1_N

Protein Analysis

341

Amino Acids

35.45

Weight (kDa)

8.3

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010945)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G53240 AT3G15020 AT3G15020
fragaria_vesca FvH4_3g18670
malus_domestica MD03G1240100.v1.1 MD11G1259400.v1.1
prunus_persica Prupe.4G058400_v2.0.a1 Prupe.4G170500_v2.0.a1
pyrus_communis pycom03g18530 pycom11g22920
rosa_chinensis RchiOBHm_Chr5g0031491
rosa_laevigata RLG00000033315
rosa_multiflora Rmu_sc0006344.1_g000010
rosa_roxburghii Rroxscaffold_1G00048830
rosa_rugosa Rorug05G0126300
rosa_samantha Rh5AG219200 Rh5BG218600 Rh5CG242200 Rh5DG224100
rosa_wichuraiana Rw5G019990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 649
AatII GACGTC 1 cut(s) 210
AccII CGCG 2 cut(s) 334, 336
AciI CCGC 4 cut(s) 74, 102, 458, 461
AclI AACGTT 1 cut(s) 564
AclWI GGATC 2 cut(s) 289, 751
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 2 cut(s) 195, 207
AfaI GTAC 2 cut(s) 56, 173
AfeI AGCGCT 2 cut(s) 273, 382
AfiI CCNNNNNNNGG 4 cut(s) 232, 457, 596, 771
AgsI TTSAA 6 cut(s) 349, 444, 472, 725, 799, 830
AjiI CACGTC 1 cut(s) 217
AluBI AGCT 6 cut(s) 142, 262, 502, 560, 683, 976
AluI AGCT 6 cut(s) 142, 262, 502, 560, 683, 976
Alw26I GTCTC 2 cut(s) 160, 241
AlwI GGATC 2 cut(s) 289, 751
Ama87I CYCGRG 1 cut(s) 238
Aor51HI AGCGCT 2 cut(s) 273, 382
AoxI GGCC 5 cut(s) 115, 303, 570, 762, 952
ApeKI GCWGC 4 cut(s) 76, 200, 259, 383
ApoI RAATTY 1 cut(s) 439
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AspLEI GCGC 4 cut(s) 81, 274, 336, 383
AspS9I GGNCC 3 cut(s) 448, 926, 937
AsuC2I CCSGG 4 cut(s) 192, 302, 324, 772
AsuHPI GGTGA 3 cut(s) 79, 448, 523
AvaI CYCGRG 1 cut(s) 238
AvaII GGWCC 3 cut(s) 448, 926, 937
BauI CACGAG 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 662
BbvI GCAGC 4 cut(s) 88, 187, 271, 370
BccI CCATC 2 cut(s) 626, 698
BceAI ACGGC 1 cut(s) 29
BcnI CCSGG 4 cut(s) 192, 302, 324, 772
BcoDI GTCTC 2 cut(s) 160, 241
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 2 cut(s) 591, 987
BfoI RGCGCY 2 cut(s) 275, 384
BisI GCNGC 7 cut(s) 74, 77, 102, 201, 260, 384, 459
BlsI GCNGC 7 cut(s) 75, 78, 103, 202, 261, 385, 460
Bme1390I CCNGG 4 cut(s) 192, 302, 324, 772
Bme18I GGWCC 3 cut(s) 448, 926, 937
BmeT110I CYCGRG 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 3 cut(s) 448, 926, 937
BmiI GGNNCC 4 cut(s) 100, 450, 816, 927
BmrFI CCNGG 4 cut(s) 192, 302, 324, 772
BmsI GCATC 2 cut(s) 372, 778
BoxI GACNNNNGTC 1 cut(s) 931
BpiI GAAGAC 1 cut(s) 662
BplI GAGNNNNNCTC 3 cut(s) 27, 16, 48
Bpu10I CCTNAGC 2 cut(s) 231, 972
BpuEI CTTGAG 2 cut(s) 296, 621
BpuMI CCSGG 4 cut(s) 192, 302, 324, 772
BsaHI GRCGYC 2 cut(s) 195, 207
BsaI GGTCTC 1 cut(s) 241
BsaJI CCNNGG 2 cut(s) 771, 949
BsaWI WCCGGW 1 cut(s) 433
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 457, 596, 771
Bse118I RCCGGY 4 cut(s) 182, 248, 305, 359
Bse3DI GCAATG 1 cut(s) 387
BseDI CCNNGG 2 cut(s) 771, 949
BseGI GGATG 4 cut(s) 297, 489, 618, 673
BseLI CCNNNNNNNGG 4 cut(s) 232, 457, 596, 771
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 3 cut(s) 5, 245, 963
BseRI GAGGAG 2 cut(s) 122, 148
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 4 cut(s) 88, 187, 271, 370
Bsh1236I CGCG 2 cut(s) 334, 336
Bsh1285I CGRYCG 2 cut(s) 53, 306
BshFI GGCC 5 cut(s) 117, 305, 572, 764, 954
BsiEI CGRYCG 2 cut(s) 53, 306
BsiHKCI CYCGRG 1 cut(s) 238
BslFI GGGAC 4 cut(s) 387, 434, 724, 939
BslI CCNNNNNNNGG 4 cut(s) 232, 457, 596, 771
BsmAI GTCTC 2 cut(s) 160, 241
BsmBI CGTCTC 1 cut(s) 160
BsmFI GGGAC 4 cut(s) 387, 434, 724, 939
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 5 cut(s) 117, 305, 572, 764, 954
Bso31I GGTCTC 1 cut(s) 241
BsoBI CYCGRG 1 cut(s) 238
Bsp143I GATC 5 cut(s) 50, 281, 340, 675, 743
BspACI CCGC 4 cut(s) 74, 102, 458, 461
BspANI GGCC 5 cut(s) 117, 305, 572, 764, 954
BspCNI CTCAG 3 cut(s) 6, 244, 964
BspFNI CGCG 2 cut(s) 334, 336
BspHI TCATGA 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 100, 450, 816, 927
BspPI GGATC 2 cut(s) 289, 751
BspQI GCTCTTC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 387
BsrFI RCCGGY 4 cut(s) 182, 248, 305, 359
BssAI RCCGGY 4 cut(s) 182, 248, 305, 359
BssECI CCNNGG 2 cut(s) 771, 949
BssMI GATC 5 cut(s) 50, 281, 340, 675, 743
BssNI GRCGYC 2 cut(s) 195, 207
BssSI CACGAG 1 cut(s) 941
BssT1I CCWWGG 1 cut(s) 949
Bst2BI CACGAG 1 cut(s) 941
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 2 cut(s) 249, 666
BstACI GRCGYC 2 cut(s) 195, 207
BstC8I GCNNGC 7 cut(s) 119, 184, 250, 307, 361, 553, 793
BstDEI CTNAG 3 cut(s) 14, 231, 972
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 4 cut(s) 297, 489, 618, 673
BstFNI CGCG 2 cut(s) 334, 336
BstH2I RGCGCY 2 cut(s) 275, 384
BstHHI GCGC 4 cut(s) 81, 274, 336, 383
BstKTI GATC 5 cut(s) 53, 284, 343, 678, 746
BstMAI GTCTC 2 cut(s) 160, 241
BstMBI GATC 5 cut(s) 50, 281, 340, 675, 743
BstMCI CGRYCG 2 cut(s) 53, 306
BstMWI GCNNNNNNNGC 5 cut(s) 127, 192, 389, 557, 782
BstNSI RCATGY 1 cut(s) 22
BstPAI GACNNNNGTC 1 cut(s) 931
BstSCI CCNGG 4 cut(s) 190, 300, 322, 770
BstSFI CTRYAG 2 cut(s) 591, 987
BstUI CGCG 2 cut(s) 334, 336
BstV1I GCAGC 4 cut(s) 88, 187, 271, 370
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 2 cut(s) 281, 743
BstXI CCANNNNNNTGG 1 cut(s) 412
BstYI RGATCY 2 cut(s) 281, 743
BstZI CGGCCG 1 cut(s) 303
BsuRI GGCC 5 cut(s) 117, 305, 572, 764, 954
BtgZI GCGATG 1 cut(s) 351
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 4 cut(s) 297, 489, 618, 673
Cac8I GCNNGC 7 cut(s) 119, 184, 250, 307, 361, 553, 793
CciI TCATGA 1 cut(s) 135
CfoI GCGC 4 cut(s) 81, 274, 336, 383
Cfr10I RCCGGY 4 cut(s) 182, 248, 305, 359
Cfr13I GGNCC 3 cut(s) 448, 926, 937
CseI GACGC 2 cut(s) 184, 340
Csp6I GTAC 2 cut(s) 55, 172
CviAII CATG 3 cut(s) 19, 136, 605
CviQI GTAC 2 cut(s) 55, 172
DdeI CTNAG 3 cut(s) 14, 231, 972
DpnI GATC 5 cut(s) 52, 283, 342, 677, 745
DpnII GATC 5 cut(s) 50, 281, 340, 675, 743
DrdI GACNNNNNNGTC 1 cut(s) 649
DseDI GACNNNNNNGTC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 303
EagI CGGCCG 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 249, 666
EarI CTCTTC 2 cut(s) 249, 666
EciI GGCGGA 1 cut(s) 476
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 1 cut(s) 949
Eco147I AGGCCT 2 cut(s) 572, 954
Eco31I GGTCTC 1 cut(s) 241
Eco47I GGWCC 3 cut(s) 448, 926, 937
Eco47III AGCGCT 2 cut(s) 273, 382
Eco52I CGGCCG 1 cut(s) 303
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 926
EcoRI GAATTC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 949
ErhI CCWWGG 1 cut(s) 949
Esp3I CGTCTC 1 cut(s) 160
FaeI CATG 3 cut(s) 22, 139, 608
FaiI YATR 9 cut(s) 20, 113, 137, 179, 422, 549, 606, 768, 989
FaqI GGGAC 4 cut(s) 387, 434, 724, 939
FatI CATG 3 cut(s) 18, 135, 604
Fnu4HI GCNGC 7 cut(s) 74, 77, 102, 201, 260, 384, 459
FokI GGATG 4 cut(s) 284, 496, 605, 680
Fsp4HI GCNGC 7 cut(s) 74, 77, 102, 201, 260, 384, 459
FspBI CTAG 1 cut(s) 890
GlaI GCGC 4 cut(s) 80, 273, 335, 382
GluI GCNGC 7 cut(s) 74, 77, 102, 201, 260, 384, 459
HaeII RGCGCY 2 cut(s) 275, 384
HaeIII GGCC 5 cut(s) 117, 305, 572, 764, 954
HgaI GACGC 2 cut(s) 184, 340
HhaI GCGC 4 cut(s) 81, 274, 336, 383
Hin1I GRCGYC 2 cut(s) 195, 207
Hin1II CATG 3 cut(s) 22, 139, 608
Hin6I GCGC 4 cut(s) 79, 272, 334, 381
HinP1I GCGC 4 cut(s) 79, 272, 334, 381
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HphI GGTGA 3 cut(s) 79, 448, 523
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 6 cut(s) 15, 50, 286, 577, 667, 937
Hpy188III TCNNGA 4 cut(s) 136, 451, 472, 818
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 2 cut(s) 200, 209
HpyAV CCTTC 5 cut(s) 469, 632, 793, 874, 988
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4IV ACGT 3 cut(s) 207, 216, 564
HpyCH4V TGCA 1 cut(s) 844
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 192, 389, 557, 782
HpyF3I CTNAG 3 cut(s) 14, 231, 972
HpySE526I ACGT 3 cut(s) 207, 216, 564
Hsp92I GRCGYC 2 cut(s) 195, 207
Hsp92II CATG 3 cut(s) 22, 139, 608
HspAI GCGC 4 cut(s) 79, 272, 334, 381
KroI GCCGGC 4 cut(s) 182, 248, 305, 359
KroNI GCCGGC 4 cut(s) 184, 250, 307, 361
Kzo9I GATC 5 cut(s) 50, 281, 340, 675, 743
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 37, 98, 135
Lsp1109I GCAGC 4 cut(s) 88, 187, 271, 370
LweI GCATC 2 cut(s) 372, 778
MaeI CTAG 1 cut(s) 890
MaeII ACGT 3 cut(s) 207, 216, 564
MaeIII GTNAC 4 cut(s) 85, 511, 640, 853
MalI GATC 5 cut(s) 52, 283, 342, 677, 745
MboI GATC 5 cut(s) 50, 281, 340, 675, 743
MboII GAAGA 4 cut(s) 266, 508, 667, 683
MflI RGATCY 2 cut(s) 281, 743
MluCI AATT 3 cut(s) 439, 777, 860
MmeI TCCRAC 2 cut(s) 645, 915
MreI CGCCGGCG 1 cut(s) 248
MroNI GCCGGC 4 cut(s) 182, 248, 305, 359
MseI TTAA 2 cut(s) 417, 978
MspR9I CCNGG 4 cut(s) 192, 302, 324, 772
Mva1269I GAATGC 1 cut(s) 836
MvnI CGCG 2 cut(s) 334, 336
MwoI GCNNNNNNNGC 5 cut(s) 127, 192, 389, 557, 782
NaeI GCCGGC 4 cut(s) 184, 250, 307, 361
NciI CCSGG 4 cut(s) 192, 302, 324, 772
NdeII GATC 5 cut(s) 50, 281, 340, 675, 743
NgoMIV GCCGGC 4 cut(s) 182, 248, 305, 359
NlaIII CATG 3 cut(s) 22, 139, 608
NlaIV GGNNCC 4 cut(s) 100, 450, 816, 927
NmeAIII GCCGAG 1 cut(s) 243
NmuCI GTSAC 3 cut(s) 85, 511, 640
NspI RCATGY 1 cut(s) 22
PagI TCATGA 1 cut(s) 135
PceI AGGCCT 2 cut(s) 572, 954
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 204, 213
PctI GAATGC 1 cut(s) 836
PdiI GCCGGC 4 cut(s) 184, 250, 307, 361
PflFI GACNNNGTC 2 cut(s) 290, 661
PkrI GCNGC 7 cut(s) 75, 78, 103, 202, 261, 385, 460
Ple19I CGATCG 1 cut(s) 53
PpuMI RGGWCCY 1 cut(s) 926
PshAI GACNNNNGTC 1 cut(s) 931
Psp1406I AACGTT 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 926
PspN4I GGNNCC 4 cut(s) 100, 450, 816, 927
PspPI GGNCC 3 cut(s) 448, 926, 937
PspPPI RGGWCCY 1 cut(s) 926
PsuI RGATCY 2 cut(s) 281, 743
PsyI GACNNNGTC 2 cut(s) 290, 661
PvuI CGATCG 1 cut(s) 53
RsaI GTAC 2 cut(s) 56, 173
RsaNI GTAC 2 cut(s) 55, 172
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 417, 978
SatI GCNGC 7 cut(s) 74, 77, 102, 201, 260, 384, 459
Sau3AI GATC 5 cut(s) 50, 281, 340, 675, 743
Sau96I GGNCC 3 cut(s) 448, 926, 937
ScrFI CCNGG 4 cut(s) 192, 302, 324, 772
SfaNI GCATC 2 cut(s) 372, 778
SfcI CTRYAG 2 cut(s) 591, 987
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 3 cut(s) 448, 926, 937
SmlI CTYRAG 2 cut(s) 275, 636
SmoI CTYRAG 2 cut(s) 275, 636
Sse9I AATT 3 cut(s) 439, 777, 860
SseBI AGGCCT 2 cut(s) 572, 954
SsiI CCGC 4 cut(s) 74, 102, 458, 461
SspMI CTAG 1 cut(s) 890
StuI AGGCCT 2 cut(s) 572, 954
StyD4I CCNGG 4 cut(s) 190, 300, 322, 770
StyI CCWWGG 1 cut(s) 949
TaaI ACNGT 1 cut(s) 448
TaiI ACGT 3 cut(s) 210, 219, 567
TaqI TCGA 3 cut(s) 489, 653, 878
TasI AATT 3 cut(s) 439, 777, 860
TauI GCSGC 3 cut(s) 76, 104, 461
Tru1I TTAA 2 cut(s) 417, 978
Tru9I TTAA 2 cut(s) 417, 978
TseFI GTSAC 3 cut(s) 85, 511, 640
TseI GCWGC 4 cut(s) 76, 200, 259, 383
Tsp45I GTSAC 3 cut(s) 85, 511, 640
TspDTI ATGAA 2 cut(s) 152, 684
Tth111I GACNNNGTC 2 cut(s) 290, 661
VpaK11BI GGWCC 3 cut(s) 448, 926, 937
XagI CCTNNNNNAGG 1 cut(s) 230
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 22
XspI CTAG 1 cut(s) 890
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.