Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0010945

Physical Location & Seq

Genomic Coordinates Forward (+)

25130993 .. 25133651

Gene Structure 2659 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG224100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGAGGCCATCCATGTCCTTGGTCAGATCCGTCCACCGCGTCGCCCGCCGCGCCTACTCCTCGGAGTCCGTCCCCCACCGCAAGGTCGCCGTTCTCGGCGCCGCCGGCGGCATCGGCCAGCCCCTCGCCCTCCTCATGAAGCTCAACCCTCTCGTCTCCCACCTCTCCCTCTACGATATCGCCGGCACCCCCGGCGTCGCCGCCGACGTCAGCCACATCAACACCAGATCTGAGGTTAAGGGTTACGCTGGGGAGGATCAGCTCGCCGAGGCCTTGGAGGGATGCGACGTCGTGATTATCCCCGCCGGTGTGCCACGTAAGCCTGGAATGACACGTGACGATCTCTTCAACATTAATGCTGGCATCGTCAAGTCTCTGACCTCCGCCATCGCCAAGTACTGCCCTCATGCGATTATCAACATGATTAGCAACCCTGTTAACTCCACCGTGCCCATCGCATCGGAGGTGTTGAAGAAGGCGGGGGTTTACGATGAGAAGAGGTTGTTTGGTGTGACTACTCTTGATGTGGTCAGGGCCAAGACTTTCTATGCTGGCAAGGCCAATGTCCCTGTTGCTGAGGTTAATGTCCCAGTTGTTGGTGGCCACGCTGGAATTACCATTCTCCCACTATTTTCTCAGGCTACTCCAAAAGCCAATTTGCCTGATGATTACATCAAGGCCTTGACAAAGAGAACCCAGGACGGAGGGACAGAAGTTGTGGAAGCCAAAGCTGGAAAGGGATCTGCTACATTGTCTATGGCTTATGCTGGAGCTCTTTTTGCTGATGCTTGTCTGAAGGGACTTAATGGGGTCCCAGATGTTGTGGAATGTTCGTATGTGCAATCGAGCATCACTGAACTTCCTTTCTTTGCTTCCAAGGTTAGTGAGCTTAACAAAATTGGGATTTCATTTCTCTCAAGCTTTTTAAGGTTTCTTAATGCACCAGTAATTGGCTTTATAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0016020 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0030060 GO:0030312 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

321

Amino Acids

33.77

Weight (kDa)

9.06

Isoelectric Point (pI)

44.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	28 - 170	2.2e-48	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	172 - 295	2.9e-35	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53240 AT3G15020 AT3G15020
fragaria_vesca	FvH4_3g18670
malus_domestica	MD03G1240100.v1.1 MD11G1259400.v1.1
prunus_persica	Prupe.4G058400_v2.0.a1 Prupe.4G170500_v2.0.a1
pyrus_communis	pycom03g18530 pycom11g22920
rosa_chinensis	RchiOBHm_Chr5g0031491
rosa_laevigata	RLG00000033315
rosa_multiflora	Rmu_sc0006344.1_g000010
rosa_roxburghii	Rroxscaffold_1G00048830
rosa_rugosa	Rorug05G0126300
rosa_samantha	Rh5AG219200 Rh5BG218600 Rh5CG242200 Rh5DG224100
rosa_wichuraiana	Rw5G019990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	210, 291
AccB1I	GGYRCC	2 cut(s)	98, 185
AccB7I	CCANNNNNTGG	2 cut(s)	596, 950
AccII	CGCG	2 cut(s)	39, 51
AclWI	GGATC	3 cut(s)	21, 264, 748
AcoI	YGGCCR	2 cut(s)	115, 601
AcuI	CTGAAG	1 cut(s)	815
AcvI	CACGTG	1 cut(s)	335
AcyI	GRCGYC	4 cut(s)	99, 195, 207, 288
AfaI	GTAC	1 cut(s)	398
AfiI	CCNNNNNNNGG	3 cut(s)	82, 596, 950
AflIII	ACRYGT	1 cut(s)	332
AgsI	TTSAA	2 cut(s)	349, 472
AjnI	CCWGG	2 cut(s)	322, 696
AluBI	AGCT	7 cut(s)	142, 262, 731, 773, 889, 921, 962
AluI	AGCT	7 cut(s)	142, 262, 731, 773, 889, 921, 962
Alw21I	GWGCWC	1 cut(s)	775
Alw26I	GTCTC	2 cut(s)	160, 378
AlwI	GGATC	3 cut(s)	21, 264, 748
AoxI	GGCC	7 cut(s)	5, 115, 270, 534, 558, 601, 678
AseI	ATTAAT	1 cut(s)	354
AspLEI	GCGC	2 cut(s)	53, 101
AspS9I	GGNCC	2 cut(s)	534, 811
AsuC2I	CCSGG	1 cut(s)	192
AvaII	GGWCC	1 cut(s)	811
BaeGI	GKGCMC	1 cut(s)	453
BalI	TGGCCA	1 cut(s)	603
BanI	GGYRCC	2 cut(s)	98, 185
BanII	GRGCYC	1 cut(s)	775
BbrPI	CACGTG	1 cut(s)	335
Bbv12I	GWGCWC	1 cut(s)	775
BbvCI	CCTCAGC	1 cut(s)	576
BccI	CCATC	3 cut(s)	16, 395, 461
BceAI	ACGGC	1 cut(s)	74
BciT130I	CCWGG	2 cut(s)	324, 698
BcnI	CCSGG	1 cut(s)	192
BcoDI	GTCTC	2 cut(s)	160, 378
BfoI	RGCGCY	1 cut(s)	102
BglII	AGATCT	1 cut(s)	227
BisI	GCNGC	4 cut(s)	49, 102, 109, 201
BlsI	GCNGC	4 cut(s)	50, 103, 110, 202
BmcAI	AGTACT	1 cut(s)	398
Bme1390I	CCNGG	3 cut(s)	192, 324, 698
Bme18I	GGWCC	1 cut(s)	811
BmgT120I	GGNCC	2 cut(s)	534, 811
BmiI	GGNNCC	4 cut(s)	100, 187, 812, 813
BmrFI	CCNGG	3 cut(s)	192, 324, 698
BmrI	ACTGGG	1 cut(s)	584
BmsI	GCATC	6 cut(s)	120, 272, 372, 467, 775, 858
BmuI	ACTGGG	1 cut(s)	584
BpmI	CTGGAG	1 cut(s)	789
Bpu10I	CCTNAGC	1 cut(s)	576
BpuEI	CTTGAG	1 cut(s)	901
BpuMI	CCSGG	1 cut(s)	192
BsaAI	YACGTR	2 cut(s)	317, 335
BsaHI	GRCGYC	4 cut(s)	99, 195, 207, 288
BsaJI	CCNNGG	7 cut(s)	18, 60, 190, 267, 273, 696, 876
Bsc4I	CCNNNNNNNGG	3 cut(s)	82, 596, 950
Bse118I	RCCGGY	3 cut(s)	104, 182, 305
Bse1I	ACTGG	2 cut(s)	590, 944
BseBI	CCWGG	2 cut(s)	324, 698
BseDI	CCNNGG	7 cut(s)	18, 60, 190, 267, 273, 696, 876
BseGI	GGATG	2 cut(s)	8, 287
BseLI	CCNNNNNNNGG	3 cut(s)	82, 596, 950
BseMII	CTCAG	3 cut(s)	222, 567, 650
BseNI	ACTGG	2 cut(s)	590, 944
BseRI	GAGGAG	2 cut(s)	49, 122
BseSI	GKGCMC	1 cut(s)	453
BseYI	CCCAGC	1 cut(s)	248
Bsh1236I	CGCG	2 cut(s)	39, 51
BshFI	GGCC	7 cut(s)	7, 117, 272, 536, 560, 603, 680
BshNI	GGYRCC	2 cut(s)	98, 185
BsiHKAI	GWGCWC	1 cut(s)	775
BsiSI	CCGG	4 cut(s)	105, 183, 192, 306
BslFI	GGGAC	6 cut(s)	56, 551, 572, 721, 797, 813
BslI	CCNNNNNNNGG	3 cut(s)	82, 596, 950
BsmAI	GTCTC	2 cut(s)	160, 378
BsmBI	CGTCTC	1 cut(s)	160
BsmFI	GGGAC	6 cut(s)	56, 551, 572, 721, 797, 813
BsnI	GGCC	7 cut(s)	7, 117, 272, 536, 560, 603, 680
Bsp1286I	GDGCHC	2 cut(s)	453, 775
Bsp143I	GATC	5 cut(s)	26, 227, 256, 340, 740
BspANI	GGCC	7 cut(s)	7, 117, 272, 536, 560, 603, 680
BspCNI	CTCAG	3 cut(s)	223, 568, 649
BspFNI	CGCG	2 cut(s)	39, 51
BspHI	TCATGA	1 cut(s)	135
BspLI	GGNNCC	4 cut(s)	100, 187, 812, 813
BspPI	GGATC	3 cut(s)	21, 264, 748
BspT107I	GGYRCC	2 cut(s)	98, 185
BsrFI	RCCGGY	3 cut(s)	104, 182, 305
BsrI	ACTGG	2 cut(s)	590, 944
BssAI	RCCGGY	3 cut(s)	104, 182, 305
BssECI	CCNNGG	7 cut(s)	18, 60, 190, 267, 273, 696, 876
BssMI	GATC	5 cut(s)	26, 227, 256, 340, 740
BssNI	GRCGYC	4 cut(s)	99, 195, 207, 288
BssT1I	CCWWGG	3 cut(s)	18, 273, 876
Bst2UI	CCWGG	2 cut(s)	324, 698
Bst4CI	ACNGT	1 cut(s)	448
Bst6I	CTCTTC	2 cut(s)	350, 491
BstACI	GRCGYC	4 cut(s)	99, 195, 207, 288
BstBAI	YACGTR	2 cut(s)	317, 335
BstC8I	GCNNGC	7 cut(s)	46, 106, 119, 184, 264, 361, 553
BstDEI	CTNAG	4 cut(s)	231, 576, 636, 963
BstF5I	GGATG	2 cut(s)	8, 287
BstFNI	CGCG	2 cut(s)	39, 51
BstH2I	RGCGCY	1 cut(s)	102
BstHHI	GCGC	2 cut(s)	53, 101
BstKTI	GATC	5 cut(s)	29, 230, 259, 343, 743
BstMAI	GTCTC	2 cut(s)	160, 378
BstMBI	GATC	5 cut(s)	26, 227, 256, 340, 740
BstMWI	GCNNNNNNNGC	8 cut(s)	45, 50, 105, 114, 192, 319, 557, 779
BstNI	CCWGG	2 cut(s)	324, 698
BstSCI	CCNGG	3 cut(s)	190, 322, 696
BstSLI	GKGCMC	1 cut(s)	453
BstUI	CGCG	2 cut(s)	39, 51
BstX2I	RGATCY	3 cut(s)	26, 227, 740
BstXI	CCANNNNNNTGG	1 cut(s)	19
BstYI	RGATCY	3 cut(s)	26, 227, 740
BsuRI	GGCC	7 cut(s)	7, 117, 272, 536, 560, 603, 680
BtgZI	GCGATG	2 cut(s)	373, 439
BtsCI	GGATG	2 cut(s)	8, 287
BtsIMutI	CAGTG	1 cut(s)	852
Cac8I	GCNNGC	7 cut(s)	46, 106, 119, 184, 264, 361, 553
CciI	TCATGA	1 cut(s)	135
CfoI	GCGC	2 cut(s)	53, 101
Cfr10I	RCCGGY	3 cut(s)	104, 182, 305
Cfr13I	GGNCC	2 cut(s)	534, 811
CseI	GACGC	2 cut(s)	28, 184
Csp6I	GTAC	1 cut(s)	397
CviAII	CATG	4 cut(s)	13, 136, 407, 421
CviQI	GTAC	1 cut(s)	397
DdeI	CTNAG	4 cut(s)	231, 576, 636, 963
DinI	GGCGCC	1 cut(s)	100
DpnI	GATC	5 cut(s)	28, 229, 258, 342, 742
DpnII	GATC	5 cut(s)	26, 227, 256, 340, 740
EaeI	YGGCCR	2 cut(s)	115, 601
Eam1104I	CTCTTC	2 cut(s)	350, 491
EarI	CTCTTC	2 cut(s)	350, 491
EciI	GGCGGA	1 cut(s)	373
Ecl136II	GAGCTC	1 cut(s)	773
Eco130I	CCWWGG	3 cut(s)	18, 273, 876
Eco147I	AGGCCT	2 cut(s)	272, 680
Eco24I	GRGCYC	1 cut(s)	775
Eco32I	GATATC	1 cut(s)	178
Eco47I	GGWCC	1 cut(s)	811
Eco53kI	GAGCTC	1 cut(s)	773
Eco57I	CTGAAG	1 cut(s)	815
Eco72I	CACGTG	1 cut(s)	335
EcoICRI	GAGCTC	1 cut(s)	773
EcoO109I	RGGNCCY	1 cut(s)	811
EcoRII	CCWGG	2 cut(s)	322, 696
EcoRV	GATATC	1 cut(s)	178
EcoT14I	CCWWGG	3 cut(s)	18, 273, 876
EcoT38I	GRGCYC	1 cut(s)	775
EgeI	GGCGCC	1 cut(s)	100
EheI	GGCGCC	1 cut(s)	100
ErhI	CCWWGG	3 cut(s)	18, 273, 876
Esp3I	CGTCTC	1 cut(s)	160
FaeI	CATG	4 cut(s)	16, 139, 410, 424
FaiI	YATR	9 cut(s)	14, 137, 408, 422, 549, 758, 765, 837, 959
FaqI	GGGAC	6 cut(s)	56, 551, 572, 721, 797, 813
FatI	CATG	4 cut(s)	12, 135, 406, 420
FauI	CCCGC	3 cut(s)	53, 310, 472
Fnu4HI	GCNGC	4 cut(s)	49, 102, 109, 201
FokI	GGATG	1 cut(s)	294
FriOI	GRGCYC	1 cut(s)	775
Fsp4HI	GCNGC	4 cut(s)	49, 102, 109, 201
GlaI	GCGC	2 cut(s)	52, 100
GluI	GCNGC	4 cut(s)	49, 102, 109, 201
GsaI	CCCAGC	1 cut(s)	252
GsuI	CTGGAG	1 cut(s)	789
HaeII	RGCGCY	1 cut(s)	102
HaeIII	GGCC	7 cut(s)	7, 117, 272, 536, 560, 603, 680
HapII	CCGG	4 cut(s)	105, 183, 192, 306
HgaI	GACGC	2 cut(s)	28, 184
HhaI	GCGC	2 cut(s)	53, 101
Hin1I	GRCGYC	4 cut(s)	99, 195, 207, 288
Hin1II	CATG	4 cut(s)	16, 139, 410, 424
Hin6I	GCGC	2 cut(s)	51, 99
HinP1I	GCGC	2 cut(s)	51, 99
HincII	GTYRAC	1 cut(s)	439
HindII	GTYRAC	1 cut(s)	439
HindIII	AAGCTT	1 cut(s)	919
HinfI	GANTC	1 cut(s)	65
HpaI	GTTAAC	1 cut(s)	439
HpaII	CCGG	4 cut(s)	105, 183, 192, 306
Hpy166II	GTNNAC	3 cut(s)	34, 439, 487
Hpy188I	TCNGA	6 cut(s)	26, 64, 232, 378, 463, 795
Hpy188III	TCNNGA	3 cut(s)	136, 292, 521
Hpy8I	GTNNAC	3 cut(s)	34, 439, 487
Hpy99I	CGWCG	5 cut(s)	44, 200, 209, 290, 293
HpyAV	CCTTC	2 cut(s)	469, 790
HpyCH4III	ACNGT	1 cut(s)	448
HpyCH4IV	ACGT	4 cut(s)	207, 288, 316, 334
HpyCH4V	TGCA	2 cut(s)	841, 941
HpyF10VI	GCNNNNNNNGC	8 cut(s)	45, 50, 105, 114, 192, 319, 557, 779
HpyF3I	CTNAG	4 cut(s)	231, 576, 636, 963
HpySE526I	ACGT	4 cut(s)	207, 288, 316, 334
Hsp92I	GRCGYC	4 cut(s)	99, 195, 207, 288
Hsp92II	CATG	4 cut(s)	16, 139, 410, 424
HspAI	GCGC	2 cut(s)	51, 99
KasI	GGCGCC	1 cut(s)	98
KflI	GGGWCCC	1 cut(s)	811
KroI	GCCGGC	2 cut(s)	104, 182
KroNI	GCCGGC	2 cut(s)	106, 184
KspAI	GTTAAC	1 cut(s)	439
Kzo9I	GATC	5 cut(s)	26, 227, 256, 340, 740
LmnI	GCTCC	1 cut(s)	770
LweI	GCATC	6 cut(s)	120, 272, 372, 467, 775, 858
MaeII	ACGT	4 cut(s)	207, 288, 316, 334
MaeIII	GTNAC	3 cut(s)	242, 335, 511
MalI	GATC	5 cut(s)	28, 229, 258, 342, 742
MboI	GATC	5 cut(s)	26, 227, 256, 340, 740
MboII	GAAGA	3 cut(s)	337, 484, 508
MflI	RGATCY	3 cut(s)	26, 227, 740
MhlI	GDGCHC	2 cut(s)	453, 775
MlsI	TGGCCA	1 cut(s)	603
MluCI	AATT	4 cut(s)	612, 655, 897, 948
MluNI	TGGCCA	1 cut(s)	603
Mly113I	GGCGCC	1 cut(s)	99
MlyI	GAGTC	1 cut(s)	74
Mox20I	TGGCCA	1 cut(s)	603
MreI	CGCCGGCG	1 cut(s)	104
MroNI	GCCGGC	2 cut(s)	104, 182
MscI	TGGCCA	1 cut(s)	603
MseI	TTAA	8 cut(s)	237, 354, 438, 582, 804, 891, 926, 936
Msp20I	TGGCCA	1 cut(s)	603
MspI	CCGG	4 cut(s)	105, 183, 192, 306
MspR9I	CCNGG	3 cut(s)	192, 324, 698
MvaI	CCWGG	2 cut(s)	324, 698
MvnI	CGCG	2 cut(s)	39, 51
MwoI	GCNNNNNNNGC	8 cut(s)	45, 50, 105, 114, 192, 319, 557, 779
NaeI	GCCGGC	2 cut(s)	106, 184
NarI	GGCGCC	1 cut(s)	99
NciI	CCSGG	1 cut(s)	192
NdeII	GATC	5 cut(s)	26, 227, 256, 340, 740
NgoMIV	GCCGGC	2 cut(s)	104, 182
NlaIII	CATG	4 cut(s)	16, 139, 410, 424
NlaIV	GGNNCC	4 cut(s)	100, 187, 812, 813
NmeAIII	GCCGAG	2 cut(s)	75, 292
NmuCI	GTSAC	2 cut(s)	335, 511
PagI	TCATGA	1 cut(s)	135
PceI	AGGCCT	2 cut(s)	272, 680
PcsI	WCGNNNNNNNCGW	1 cut(s)	204
PdiI	GCCGGC	2 cut(s)	106, 184
PflMI	CCANNNNNTGG	2 cut(s)	596, 950
PkrI	GCNGC	4 cut(s)	50, 103, 110, 202
PleI	GAGTC	1 cut(s)	73
PluTI	GGCGCC	1 cut(s)	102
PmaCI	CACGTG	1 cut(s)	335
PmlI	CACGTG	1 cut(s)	335
PpsI	GAGTC	1 cut(s)	73
Ppu21I	YACGTR	2 cut(s)	317, 335
PpuMI	RGGWCCY	1 cut(s)	811
PshBI	ATTAAT	1 cut(s)	354
Psp124BI	GAGCTC	1 cut(s)	775
Psp5II	RGGWCCY	1 cut(s)	811
Psp6I	CCWGG	2 cut(s)	322, 696
PspCI	CACGTG	1 cut(s)	335
PspFI	CCCAGC	1 cut(s)	248
PspGI	CCWGG	2 cut(s)	322, 696
PspN4I	GGNNCC	4 cut(s)	100, 187, 812, 813
PspPI	GGNCC	2 cut(s)	534, 811
PspPPI	RGGWCCY	1 cut(s)	811
PsuI	RGATCY	3 cut(s)	26, 227, 740
RsaI	GTAC	1 cut(s)	398
RsaNI	GTAC	1 cut(s)	397
SacI	GAGCTC	1 cut(s)	775
SaqAI	TTAA	8 cut(s)	237, 354, 438, 582, 804, 891, 926, 936
SatI	GCNGC	4 cut(s)	49, 102, 109, 201
Sau3AI	GATC	5 cut(s)	26, 227, 256, 340, 740
Sau96I	GGNCC	2 cut(s)	534, 811
ScaI	AGTACT	1 cut(s)	398
SchI	GAGTC	1 cut(s)	74
ScrFI	CCNGG	3 cut(s)	192, 324, 698
SduI	GDGCHC	2 cut(s)	453, 775
SfaNI	GCATC	6 cut(s)	120, 272, 372, 467, 775, 858
SfoI	GGCGCC	1 cut(s)	100
SgrAI	CRCCGGYG	2 cut(s)	104, 305
SinI	GGWCC	1 cut(s)	811
SmlI	CTYRAG	1 cut(s)	916
SmoI	CTYRAG	1 cut(s)	916
Sse9I	AATT	4 cut(s)	612, 655, 897, 948
SseBI	AGGCCT	2 cut(s)	272, 680
SspDI	GGCGCC	1 cut(s)	98
SstI	GAGCTC	1 cut(s)	775
StuI	AGGCCT	2 cut(s)	272, 680
StyD4I	CCNGG	3 cut(s)	190, 322, 696
StyI	CCWWGG	3 cut(s)	18, 273, 876
TaaI	ACNGT	1 cut(s)	448
TaiI	ACGT	4 cut(s)	210, 291, 319, 337
TaqI	TCGA	1 cut(s)	845
TasI	AATT	4 cut(s)	612, 655, 897, 948
TatI	WGTACW	1 cut(s)	396
TauI	GCSGC	4 cut(s)	51, 104, 111, 203
Tru1I	TTAA	8 cut(s)	237, 354, 438, 582, 804, 891, 926, 936
Tru9I	TTAA	8 cut(s)	237, 354, 438, 582, 804, 891, 926, 936
TscAI	CASTG	1 cut(s)	859
TseFI	GTSAC	2 cut(s)	335, 511
Tsp45I	GTSAC	2 cut(s)	335, 511
TspDTI	ATGAA	2 cut(s)	152, 897
TspGWI	ACGGA	3 cut(s)	19, 58, 717
TspRI	CASTG	1 cut(s)	859
Van91I	CCANNNNNTGG	2 cut(s)	596, 950
VpaK11BI	GGWCC	1 cut(s)	811
VspI	ATTAAT	1 cut(s)	354
ZraI	GACGTC	2 cut(s)	208, 289
ZrmI	AGTACT	1 cut(s)	398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.