Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010945

Physical Location & Seq

Genomic Coordinates Reverse (-)

32550564 .. 32554045

Gene Structure 3482 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1240100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGAGGCCTTCAATGTCCATCGCCAGATCCGTACACCGAGTCGCCCGCCGCAGCTACTCATCCGCCTCCGTCCCCGAGCGGAAGGTCGCGGTCCTCGGCGCCGCCGGCGGCATCGGCCAGCCCCTGGCTCTCCTCATGAAGCTCAACCCCCTCGTCTCTCACCTCTCCCTCTACGATATCGCCGGAACCCCCGGTGTCGCCGCTGACGTCAGCCACATCAACACCAGATCTGAGGTCAAAGGTTACGCCGGAGAAGAGCAGCTTGCTCAAGCCTTGGAGGGAGCCGACGTCGTAATTATCCCCGCCGGAGTGCCCCGAAAGCCCGGCATGACCCGGGACGATCTATTCAACATTAATGCCGGTATCGTCAAGGGTCTCACTACCGCCATCGCTAAGTACTGCCCCAATGCGCTGATCAACATGATTAGCAACCCAGTCAACTCTACAGTCCCGATTGCGGCCGAGGTGTTGAAGAAGGCCGGGAAGTACGACGAGAAGAGGTTGTTTGGTGTCACTACTCTTGATGTGGTCAGGGCTAAGACTTTCTATGCTGGAAAGGCCAATGTTAATGTTGCTGAGGTTAATGTACCAGTTGTTGGTGGCCATGCTGGCGTCACCATTCTGCCACTATTTTCTCAGGCTACCCCAGCAGCCAATTTGCCTCATGATGTTATTGTGGCTTTGACAAAGAGAACACAAGATGGAGGAACGGAAGTTGTGGAGGCGAAGGCTGGAAAGGGATCAGCAACATTGTCAATGGCCTATGCTGGAGCTATTTTCGCTGATGCTTGTCTGAAGGGACTTAATGGGGTACCCGATGTTGTGGAATGTTCGTATGTGCAATCAAGCATCACTGAACTTCCTTTCTTTGCTTCTAAGGTGAGGCTTGGAAAGAATGGAGTCGAGGAAGTTTTGGGGTTGGGTAATCTCTCAGACTTTGAACAGGAAGGACTCCAAAAATTGATCCCTGAGCTCAAATCATCCATCGAGAAGGGTATCCGGTTTGCCAACCAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0016020 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0030060 GO:0030312 GO:0042221 GO:0042742 GO:0043167 GO:0043169 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0051704 GO:0051707 GO:0055114 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

340

Amino Acids

35.51

Weight (kDa)

8.98

Isoelectric Point (pI)

43.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	28 - 170	7.9e-49	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	172 - 335	1.2e-48	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010945)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53240 AT3G15020 AT3G15020
fragaria_vesca	FvH4_3g18670
malus_domestica	MD03G1240100.v1.1 MD11G1259400.v1.1
prunus_persica	Prupe.4G058400_v2.0.a1 Prupe.4G170500_v2.0.a1
pyrus_communis	pycom03g18530 pycom11g22920
rosa_chinensis	RchiOBHm_Chr5g0031491
rosa_laevigata	RLG00000033315
rosa_multiflora	Rmu_sc0006344.1_g000010
rosa_roxburghii	Rroxscaffold_1G00048830
rosa_rugosa	Rorug05G0126300
rosa_samantha	Rh5AG219200 Rh5BG218600 Rh5CG242200 Rh5DG224100
rosa_wichuraiana	Rw5G019990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	210, 291
Acc65I	GGTACC	1 cut(s)	811
AccB1I	GGYRCC	2 cut(s)	98, 811
AccB7I	CCANNNNNTGG	2 cut(s)	124, 596
AccBSI	CCGCTC	1 cut(s)	79
AccII	CGCG	1 cut(s)	89
AclWI	GGATC	3 cut(s)	21, 748, 958
AcoI	YGGCCR	3 cut(s)	115, 459, 601
AcuI	CTGAAG	1 cut(s)	815
AcyI	GRCGYC	4 cut(s)	99, 207, 288, 612
AfaI	GTAC	5 cut(s)	33, 398, 488, 588, 813
AfiI	CCNNNNNNNGG	3 cut(s)	124, 457, 596
AgsI	TTSAA	4 cut(s)	12, 349, 472, 941
AjnI	CCWGG	1 cut(s)	123
AluBI	AGCT	5 cut(s)	54, 142, 262, 773, 973
AluI	AGCT	5 cut(s)	54, 142, 262, 773, 973
Alw21I	GWGCWC	1 cut(s)	975
Alw26I	GTCTC	2 cut(s)	160, 380
AlwI	GGATC	3 cut(s)	21, 748, 958
AlwNI	CAGNNNCTG	1 cut(s)	124
Ama87I	CYCGRG	2 cut(s)	74, 333
AoxI	GGCC	7 cut(s)	5, 115, 459, 477, 558, 601, 759
ApeKI	GCWGC	3 cut(s)	51, 259, 650
AseI	ATTAAT	1 cut(s)	354
Asp718I	GGTACC	1 cut(s)	811
AspLEI	GCGC	2 cut(s)	101, 412
AspS9I	GGNCC	1 cut(s)	91
AsuC2I	CCSGG	5 cut(s)	192, 324, 334, 335, 481
AsuHPI	GGTGA	3 cut(s)	152, 607, 892
AvaI	CYCGRG	2 cut(s)	74, 333
AvaII	GGWCC	1 cut(s)	91
BaeGI	GKGCMC	1 cut(s)	315
BalI	TGGCCA	1 cut(s)	603
BanI	GGYRCC	2 cut(s)	98, 811
BanII	GRGCYC	1 cut(s)	975
Bbv12I	GWGCWC	1 cut(s)	975
BbvCI	CCTCAGC	1 cut(s)	576
BbvI	GCAGC	3 cut(s)	63, 271, 662
BccI	CCATC	4 cut(s)	26, 395, 695, 992
BciT130I	CCWGG	1 cut(s)	125
BciVI	GTATCC	1 cut(s)	1007
BclI	TGATCA	1 cut(s)	414
BcnI	CCSGG	5 cut(s)	192, 324, 334, 335, 481
BcoDI	GTCTC	2 cut(s)	160, 380
BfmI	CTRYAG	1 cut(s)	444
BfoI	RGCGCY	1 cut(s)	102
BfuI	GTATCC	1 cut(s)	1007
BglI	GCCNNNNNGGC	1 cut(s)	609
BglII	AGATCT	1 cut(s)	227
BisI	GCNGC	8 cut(s)	49, 52, 102, 109, 201, 260, 459, 651
BlsI	GCNGC	8 cut(s)	50, 53, 103, 110, 202, 261, 460, 652
BmcAI	AGTACT	1 cut(s)	398
Bme1390I	CCNGG	6 cut(s)	125, 192, 324, 334, 335, 481
Bme18I	GGWCC	1 cut(s)	91
BmeT110I	CYCGRG	2 cut(s)	74, 333
BmgT120I	GGNCC	1 cut(s)	91
BmiI	GGNNCC	4 cut(s)	100, 187, 283, 813
BmrFI	CCNGG	6 cut(s)	125, 192, 324, 334, 335, 481
BmrI	ACTGGG	1 cut(s)	428
BmsI	GCATC	3 cut(s)	120, 775, 858
BmuI	ACTGGG	1 cut(s)	428
BpmI	CTGGAG	1 cut(s)	789
Bpu10I	CCTNAGC	2 cut(s)	576, 969
BpuEI	CTTGAG	1 cut(s)	252
BpuMI	CCSGG	5 cut(s)	192, 324, 334, 335, 481
BsaHI	GRCGYC	4 cut(s)	99, 207, 288, 612
BsaI	GGTCTC	1 cut(s)	380
BsaJI	CCNNGG	6 cut(s)	94, 123, 190, 273, 333, 462
BsaWI	WCCGGW	1 cut(s)	999
BsaXI	ACNNNNNCTCC	2 cut(s)	273, 303
Bsc4I	CCNNNNNNNGG	3 cut(s)	124, 457, 596
Bse118I	RCCGGY	2 cut(s)	104, 359
Bse1I	ACTGG	2 cut(s)	434, 590
BseBI	CCWGG	1 cut(s)	125
BseDI	CCNNGG	6 cut(s)	94, 123, 190, 273, 333, 462
BseGI	GGATG	2 cut(s)	59, 980
BseLI	CCNNNNNNNGG	3 cut(s)	124, 457, 596
BseMII	CTCAG	5 cut(s)	222, 567, 650, 945, 960
BseNI	ACTGG	2 cut(s)	434, 590
BseRI	GAGGAG	1 cut(s)	122
BseSI	GKGCMC	1 cut(s)	315
BseX3I	CGGCCG	1 cut(s)	459
BseXI	GCAGC	3 cut(s)	63, 271, 662
BseYI	CCCAGC	1 cut(s)	646
Bsh1236I	CGCG	1 cut(s)	89
Bsh1285I	CGRYCG	1 cut(s)	462
BshFI	GGCC	7 cut(s)	7, 117, 461, 479, 560, 603, 761
BshNI	GGYRCC	2 cut(s)	98, 811
BsiEI	CGRYCG	1 cut(s)	462
BsiHKAI	GWGCWC	1 cut(s)	975
BsiHKCI	CYCGRG	2 cut(s)	74, 333
BslFI	GGGAC	4 cut(s)	56, 350, 434, 813
BslI	CCNNNNNNNGG	3 cut(s)	124, 457, 596
BsmAI	GTCTC	2 cut(s)	160, 380
BsmBI	CGTCTC	1 cut(s)	160
BsmFI	GGGAC	4 cut(s)	56, 350, 434, 813
BsnI	GGCC	7 cut(s)	7, 117, 461, 479, 560, 603, 761
Bso31I	GGTCTC	1 cut(s)	380
BsoBI	CYCGRG	2 cut(s)	74, 333
Bsp1286I	GDGCHC	2 cut(s)	315, 975
Bsp143I	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
BspANI	GGCC	7 cut(s)	7, 117, 461, 479, 560, 603, 761
BspCNI	CTCAG	5 cut(s)	223, 568, 649, 944, 961
BspFNI	CGCG	1 cut(s)	89
BspHI	TCATGA	2 cut(s)	135, 664
BspLI	GGNNCC	4 cut(s)	100, 187, 283, 813
BspPI	GGATC	3 cut(s)	21, 748, 958
BspQI	GCTCTTC	1 cut(s)	249
BspT107I	GGYRCC	2 cut(s)	98, 811
BspTNI	GGTCTC	1 cut(s)	380
BsrBI	CCGCTC	1 cut(s)	79
BsrFI	RCCGGY	2 cut(s)	104, 359
BsrI	ACTGG	2 cut(s)	434, 590
BssAI	RCCGGY	2 cut(s)	104, 359
BssECI	CCNNGG	6 cut(s)	94, 123, 190, 273, 333, 462
BssMI	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
BssNI	GRCGYC	4 cut(s)	99, 207, 288, 612
BssT1I	CCWWGG	1 cut(s)	273
Bst2UI	CCWGG	1 cut(s)	125
Bst4CI	ACNGT	1 cut(s)	448
Bst6I	CTCTTC	2 cut(s)	249, 491
BstACI	GRCGYC	4 cut(s)	99, 207, 288, 612
BstC8I	GCNNGC	5 cut(s)	46, 106, 119, 264, 610
BstDEI	CTNAG	8 cut(s)	231, 393, 537, 576, 636, 876, 931, 969
BstF5I	GGATG	2 cut(s)	59, 980
BstFNI	CGCG	1 cut(s)	89
BstH2I	RGCGCY	1 cut(s)	102
BstHHI	GCGC	2 cut(s)	101, 412
BstKTI	GATC	6 cut(s)	29, 230, 343, 417, 743, 966
BstMAI	GTCTC	2 cut(s)	160, 380
BstMBI	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
BstMCI	CGRYCG	1 cut(s)	462
BstMWI	GCNNNNNNNGC	7 cut(s)	105, 114, 319, 557, 609, 647, 779
BstNI	CCWGG	1 cut(s)	125
BstSCI	CCNGG	6 cut(s)	123, 190, 322, 332, 333, 479
BstSFI	CTRYAG	1 cut(s)	444
BstSLI	GKGCMC	1 cut(s)	315
BstUI	CGCG	1 cut(s)	89
BstV1I	GCAGC	3 cut(s)	63, 271, 662
BstX2I	RGATCY	2 cut(s)	26, 227
BstYI	RGATCY	2 cut(s)	26, 227
BstZI	CGGCCG	1 cut(s)	459
BsuI	GTATCC	1 cut(s)	1007
BsuRI	GGCC	7 cut(s)	7, 117, 461, 479, 560, 603, 761
BtgZI	GCGATG	2 cut(s)	4, 373
BtsCI	GGATG	2 cut(s)	59, 980
BtsIMutI	CAGTG	1 cut(s)	852
Cac8I	GCNNGC	5 cut(s)	46, 106, 119, 264, 610
CaiI	CAGNNNCTG	1 cut(s)	124
CciI	TCATGA	2 cut(s)	135, 664
CfoI	GCGC	2 cut(s)	101, 412
Cfr10I	RCCGGY	2 cut(s)	104, 359
Cfr13I	GGNCC	1 cut(s)	91
Cfr9I	CCCGGG	1 cut(s)	333
CseI	GACGC	1 cut(s)	601
Csp6I	GTAC	5 cut(s)	32, 397, 487, 587, 812
CviAII	CATG	5 cut(s)	136, 328, 421, 605, 665
CviQI	GTAC	5 cut(s)	32, 397, 487, 587, 812
DdeI	CTNAG	8 cut(s)	231, 393, 537, 576, 636, 876, 931, 969
DinI	GGCGCC	1 cut(s)	100
DpnI	GATC	6 cut(s)	28, 229, 342, 416, 742, 965
DpnII	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
EaeI	YGGCCR	3 cut(s)	115, 459, 601
EagI	CGGCCG	1 cut(s)	459
Eam1104I	CTCTTC	2 cut(s)	249, 491
EarI	CTCTTC	2 cut(s)	249, 491
EciI	GGCGGA	1 cut(s)	52
Ecl136II	GAGCTC	1 cut(s)	973
EclXI	CGGCCG	1 cut(s)	459
Eco130I	CCWWGG	1 cut(s)	273
Eco147I	AGGCCT	1 cut(s)	7
Eco24I	GRGCYC	1 cut(s)	975
Eco31I	GGTCTC	1 cut(s)	380
Eco32I	GATATC	1 cut(s)	178
Eco47I	GGWCC	1 cut(s)	91
Eco52I	CGGCCG	1 cut(s)	459
Eco53kI	GAGCTC	1 cut(s)	973
Eco57I	CTGAAG	1 cut(s)	815
Eco88I	CYCGRG	2 cut(s)	74, 333
EcoICRI	GAGCTC	1 cut(s)	973
EcoRII	CCWGG	1 cut(s)	123
EcoRV	GATATC	1 cut(s)	178
EcoT14I	CCWWGG	1 cut(s)	273
EcoT38I	GRGCYC	1 cut(s)	975
EgeI	GGCGCC	1 cut(s)	100
EheI	GGCGCC	1 cut(s)	100
ErhI	CCWWGG	1 cut(s)	273
Esp3I	CGTCTC	1 cut(s)	160
FaeI	CATG	5 cut(s)	139, 331, 424, 608, 668
FaiI	YATR	8 cut(s)	137, 329, 422, 549, 606, 666, 765, 837
FalI	AAGNNNNNCTT	2 cut(s)	246, 278
FaqI	GGGAC	4 cut(s)	56, 350, 434, 813
FatI	CATG	5 cut(s)	135, 327, 420, 604, 664
FauI	CCCGC	2 cut(s)	53, 310
FbaI	TGATCA	1 cut(s)	414
Fnu4HI	GCNGC	8 cut(s)	49, 52, 102, 109, 201, 260, 459, 651
FokI	GGATG	2 cut(s)	46, 967
FriOI	GRGCYC	1 cut(s)	975
Fsp4HI	GCNGC	8 cut(s)	49, 52, 102, 109, 201, 260, 459, 651
GlaI	GCGC	2 cut(s)	100, 411
GluI	GCNGC	8 cut(s)	49, 52, 102, 109, 201, 260, 459, 651
GsaI	CCCAGC	1 cut(s)	650
GsuI	CTGGAG	1 cut(s)	789
HaeII	RGCGCY	1 cut(s)	102
HaeIII	GGCC	7 cut(s)	7, 117, 461, 479, 560, 603, 761
HgaI	GACGC	1 cut(s)	601
HhaI	GCGC	2 cut(s)	101, 412
Hin1I	GRCGYC	4 cut(s)	99, 207, 288, 612
Hin1II	CATG	5 cut(s)	139, 331, 424, 608, 668
Hin6I	GCGC	2 cut(s)	99, 410
HinP1I	GCGC	2 cut(s)	99, 410
HincII	GTYRAC	1 cut(s)	439
HindII	GTYRAC	1 cut(s)	439
HinfI	GANTC	3 cut(s)	39, 900, 951
HphI	GGTGA	3 cut(s)	152, 607, 892
Hpy166II	GTNNAC	2 cut(s)	34, 439
Hpy188I	TCNGA	3 cut(s)	232, 795, 934
Hpy188III	TCNNGA	5 cut(s)	136, 451, 521, 665, 988
Hpy8I	GTNNAC	2 cut(s)	34, 439
Hpy99I	CGWCG	3 cut(s)	290, 293, 494
HpyAV	CCTTC	7 cut(s)	18, 76, 469, 721, 790, 941, 985
HpyCH4III	ACNGT	1 cut(s)	448
HpyCH4IV	ACGT	2 cut(s)	207, 288
HpyCH4V	TGCA	1 cut(s)	841
HpyF10VI	GCNNNNNNNGC	7 cut(s)	105, 114, 319, 557, 609, 647, 779
HpyF3I	CTNAG	8 cut(s)	231, 393, 537, 576, 636, 876, 931, 969
HpySE526I	ACGT	2 cut(s)	207, 288
Hsp92I	GRCGYC	4 cut(s)	99, 207, 288, 612
Hsp92II	CATG	5 cut(s)	139, 331, 424, 608, 668
HspAI	GCGC	2 cut(s)	99, 410
KasI	GGCGCC	1 cut(s)	98
KpnI	GGTACC	1 cut(s)	815
KroI	GCCGGC	1 cut(s)	104
KroNI	GCCGGC	1 cut(s)	106
Ksp22I	TGATCA	1 cut(s)	414
Kzo9I	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
LguI	GCTCTTC	1 cut(s)	249
LmnI	GCTCC	2 cut(s)	281, 770
Lsp1109I	GCAGC	3 cut(s)	63, 271, 662
LweI	GCATC	3 cut(s)	120, 775, 858
MaeII	ACGT	2 cut(s)	207, 288
MaeIII	GTNAC	3 cut(s)	242, 511, 613
MalI	GATC	6 cut(s)	28, 229, 342, 416, 742, 965
MbiI	CCGCTC	1 cut(s)	79
MboI	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
MboII	GAAGA	3 cut(s)	266, 484, 508
MflI	RGATCY	2 cut(s)	26, 227
MhlI	GDGCHC	2 cut(s)	315, 975
MlsI	TGGCCA	1 cut(s)	603
MluCI	AATT	3 cut(s)	294, 655, 959
MluNI	TGGCCA	1 cut(s)	603
Mly113I	GGCGCC	1 cut(s)	99
MlyI	GAGTC	3 cut(s)	48, 909, 945
Mox20I	TGGCCA	1 cut(s)	603
MreI	CGCCGGCG	1 cut(s)	104
MroNI	GCCGGC	1 cut(s)	104
MscI	TGGCCA	1 cut(s)	603
MseI	TTAA	4 cut(s)	354, 567, 582, 804
Msp20I	TGGCCA	1 cut(s)	603
MspA1I	CMGCKG	1 cut(s)	203
MspR9I	CCNGG	6 cut(s)	125, 192, 324, 334, 335, 481
MvaI	CCWGG	1 cut(s)	125
MvnI	CGCG	1 cut(s)	89
MwoI	GCNNNNNNNGC	7 cut(s)	105, 114, 319, 557, 609, 647, 779
NaeI	GCCGGC	1 cut(s)	106
NarI	GGCGCC	1 cut(s)	99
NciI	CCSGG	5 cut(s)	192, 324, 334, 335, 481
NdeII	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
NgoMIV	GCCGGC	1 cut(s)	104
NlaIII	CATG	5 cut(s)	139, 331, 424, 608, 668
NlaIV	GGNNCC	4 cut(s)	100, 187, 283, 813
NmeAIII	GCCGAG	2 cut(s)	75, 487
NmuCI	GTSAC	2 cut(s)	511, 613
PagI	TCATGA	2 cut(s)	135, 664
PceI	AGGCCT	1 cut(s)	7
PciSI	GCTCTTC	1 cut(s)	249
PcsI	WCGNNNNNNNCGW	2 cut(s)	27, 204
PdiI	GCCGGC	1 cut(s)	106
PflMI	CCANNNNNTGG	2 cut(s)	124, 596
PkrI	GCNGC	8 cut(s)	50, 53, 103, 110, 202, 261, 460, 652
PleI	GAGTC	3 cut(s)	47, 908, 945
PluTI	GGCGCC	1 cut(s)	102
PpsI	GAGTC	3 cut(s)	47, 908, 945
PshBI	ATTAAT	1 cut(s)	354
Psp124BI	GAGCTC	1 cut(s)	975
Psp6I	CCWGG	1 cut(s)	123
PspFI	CCCAGC	1 cut(s)	646
PspGI	CCWGG	1 cut(s)	123
PspN4I	GGNNCC	4 cut(s)	100, 187, 283, 813
PspPI	GGNCC	1 cut(s)	91
PstNI	CAGNNNCTG	1 cut(s)	124
PsuI	RGATCY	2 cut(s)	26, 227
RsaI	GTAC	5 cut(s)	33, 398, 488, 588, 813
RsaNI	GTAC	5 cut(s)	32, 397, 487, 587, 812
SacI	GAGCTC	1 cut(s)	975
SapI	GCTCTTC	1 cut(s)	249
SaqAI	TTAA	4 cut(s)	354, 567, 582, 804
SatI	GCNGC	8 cut(s)	49, 52, 102, 109, 201, 260, 459, 651
Sau3AI	GATC	6 cut(s)	26, 227, 340, 414, 740, 963
Sau96I	GGNCC	1 cut(s)	91
ScaI	AGTACT	1 cut(s)	398
SchI	GAGTC	3 cut(s)	48, 909, 945
ScrFI	CCNGG	6 cut(s)	125, 192, 324, 334, 335, 481
SduI	GDGCHC	2 cut(s)	315, 975
SfaNI	GCATC	3 cut(s)	120, 775, 858
SfcI	CTRYAG	1 cut(s)	444
SfoI	GGCGCC	1 cut(s)	100
SgrAI	CRCCGGYG	1 cut(s)	104
SinI	GGWCC	1 cut(s)	91
SmaI	CCCGGG	1 cut(s)	335
SmlI	CTYRAG	1 cut(s)	267
SmoI	CTYRAG	1 cut(s)	267
Sse9I	AATT	3 cut(s)	294, 655, 959
SseBI	AGGCCT	1 cut(s)	7
SspDI	GGCGCC	1 cut(s)	98
SstI	GAGCTC	1 cut(s)	975
StuI	AGGCCT	1 cut(s)	7
StyD4I	CCNGG	6 cut(s)	123, 190, 322, 332, 333, 479
StyI	CCWWGG	1 cut(s)	273
TaaI	ACNGT	1 cut(s)	448
TaiI	ACGT	2 cut(s)	210, 291
TaqI	TCGA	2 cut(s)	903, 987
TasI	AATT	3 cut(s)	294, 655, 959
TatI	WGTACW	1 cut(s)	396
TauI	GCSGC	5 cut(s)	51, 104, 111, 203, 461
Tru1I	TTAA	4 cut(s)	354, 567, 582, 804
Tru9I	TTAA	4 cut(s)	354, 567, 582, 804
TscAI	CASTG	1 cut(s)	859
TseFI	GTSAC	2 cut(s)	511, 613
TseI	GCWGC	3 cut(s)	51, 259, 650
Tsp45I	GTSAC	2 cut(s)	511, 613
TspDTI	ATGAA	1 cut(s)	152
TspGWI	ACGGA	3 cut(s)	19, 58, 725
TspMI	CCCGGG	1 cut(s)	333
TspRI	CASTG	1 cut(s)	859
Van91I	CCANNNNNTGG	2 cut(s)	124, 596
VpaK11BI	GGWCC	1 cut(s)	91
VspI	ATTAAT	1 cut(s)	354
XmaI	CCCGGG	1 cut(s)	333
ZraI	GACGTC	2 cut(s)	208, 289
ZrmI	AGTACT	1 cut(s)	398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.