AT3G18130

Guanine nucleotide-binding protein subunit beta-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010922
Physical Location & Seq
Reverse (-)
6210878 .. 6212551
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18130.1

Sequence Viewer

Length: 981 bp
ATGGCCGAGGGACTCGTATTGAAGGGCATTATGCGCGCCCACACCGACATTGTCACGGCCATCGCTACGCCGATCGACAATTCCGACATCATCGTCACAGCGTCGCGTGACAAATCCATCATCCTCTGGAAACTCACAAAGGACGATAAGTCTTACGGTGTTGCTCAGCGTAGGCTCACAGGTCACTCTCACTTCGTGGAAGATGTTGTTCTCTCATCGGACGGTCAGTTTGCACTCTCCGGAAGCTGGGACGGTGAGCTCCGTCTCTGGGATCTTGCCACGGGAGAAACAACTCGTCGATTCGTTGGTCATACGAAAGATGTGCTCTCTGTTGCATTCTCTACTGATAACCGGCAGATCGTGTCTGCTTCTCGTGATCGTACGATTAAGCTTTGGAACACACTTGGTGAGTGTAAGTATACCATCTCTGAAGGTGATGGTCACAAGGAATGGGTTAGTTGTGTTAGGTTTAGTCCTAATACTCTTGTACCAACTATTGTATCTGCTTCTTGGGATAAAACTGTGAAAGTTTGGAATCTCCAGAACTGTAAGCTGAGGAACTCTCTTGTTGGTCACTCTGGTTACCTCAACACTGTTGCTGTCTCGCCTGATGGTTCGCTATGCGCCAGTGGTGGGAAAGATGGTGTTATCTTGTTGTGGGATTTGGCTGAAGGAAAGAAGCTTTACTCGCTTGAGGCGGGTTCGATTATTCACTCGCTTTGCTTCAGTCCTAACAGATACTGGTTGTGTGCTGCTACTGAGAATAGCATTAGGATTTGGGATCTTGAGAGCAAGTCTGTTGTTGAGGACTTGAAGGTTGATCTCAAGTCTGAGGCAGAGAAGAATGAAGGTGGTGTTGGAACTGGTAACCAGAAGAAGGTTATCTACTGCACAAGCTTGAACTGGAGTGCAGATGGAAGCACATTGTTCAGTGGTTACACTGATGGAGTTGTCAGGGTCTGGGGTATTGGTCGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003735 GO:0005078 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006417 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010608 GO:0010646 GO:0010647 GO:0016020 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022613 GO:0022622 GO:0022626 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0032947 GO:0032991 GO:0033993 GO:0034248 GO:0035591 GO:0042221 GO:0042254 GO:0042325 GO:0042327 GO:0042788 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043408 GO:0043410 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045937 GO:0048364 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051704 GO:0051707 GO:0051716 GO:0060090 GO:0060255 GO:0060263 GO:0060267 GO:0065007 GO:0070013 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1990904 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko05162 map05162
Pfam Domains
Protein Families
WD40

Protein Analysis

326

Amino Acids

35.83

Weight (kDa)

6.66

Isoelectric Point (pI)

20.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 7 - 136 4.3e-16 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 10 - 161 1.2e-28 WDR5 beta-propeller domain
WD40_Prp19 PF24814 11 - 99 3.8e-09 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 11 - 99 5.2e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 11 - 140 4.2e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 11 - 223 8.7e-24 WDR3 second beta-propeller domain
Beta-prop_EML PF23409 12 - 134 3.6e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_SCAP PF24017 13 - 141 8.1e-06 SCAP Beta-propeller
WDR55 PF24796 28 - 133 3.9e-07 WDR55
Beta-prop_THOC3 PF25174 29 - 274 4.2e-40 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 55 - 192 9.9e-26 WDR3 first beta-propeller domain
WD40 PF00400 56 - 91 3.7e-08 WD domain, G-beta repeat
WD40_Gbeta PF25391 56 - 141 3.4e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 59 - 270 3.2e-45 WDR5 beta-propeller domain
EIF3I PF24805 59 - 142 1.8e-07 EIF3I
WD40_MABP1-WDR62_2nd PF24782 66 - 182 4.1e-12 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 66 - 134 1.1e-10 WDHD1 first WD40 domain
WD40_Prp19 PF24814 73 - 279 3e-37 Prp19 WD40 domain
WD40 PF00400 96 - 133 1.4e-10 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 99 - 185 8.4e-08 CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st PF24817 105 - 186 4.1e-10 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 118 - 225 6.2e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 118 - 272 5.6e-12 WDR36/Utp21 first beta-propeller
WD40 PF00400 137 - 179 8.2e-09 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 143 - 274 2.9e-11 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 144 - 272 3e-12 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 144 - 321 1.6e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 144 - 271 2e-20 WDR3 first beta-propeller domain
WDR55 PF24796 145 - 267 1.6e-08 WDR55
EIF3I PF24805 147 - 322 2.1e-14 EIF3I
Beta-prop_EML_2 PF23414 164 - 321 8.4e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_SCAP PF24017 165 - 261 3.4e-08 SCAP Beta-propeller
NBCH_WD40 PF20426 166 - 226 7.8e-06 Neurobeachin beta propeller domain
WD40 PF00400 184 - 221 2.8e-08 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 195 - 276 2.5e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccI GTMKAC 1 cut(s) 419
AccII CGCG 2 cut(s) 36, 106
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 1 cut(s) 698
AclWI GGATC 2 cut(s) 279, 789
AcoI YGGCCR 2 cut(s) 3, 57
AcuI CTGAAG 3 cut(s) 450, 690, 709
AdeI CACNNNGTG 2 cut(s) 196, 407
AfaI GTAC 2 cut(s) 382, 489
AfiI CCNNNNNNNGG 4 cut(s) 246, 268, 633, 877
AgsI TTSAA 3 cut(s) 22, 814, 901
AhdI GACNNNNNGTC 1 cut(s) 148
AjuI GAANNNNNNNTTGG 2 cut(s) 840, 872
AluBI AGCT 6 cut(s) 246, 259, 391, 553, 682, 897
AluI AGCT 6 cut(s) 246, 259, 391, 553, 682, 897
Alw21I GWGCWC 2 cut(s) 261, 327
Alw26I GTCTC 2 cut(s) 269, 607
AlwI GGATC 2 cut(s) 279, 789
AlwNI CAGNNNCTG 2 cut(s) 741, 960
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 2 cut(s) 3, 57
ApeKI GCWGC 1 cut(s) 752
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspLEI GCGC 3 cut(s) 36, 38, 626
AsuHPI GGTGA 3 cut(s) 266, 419, 446
BaeI ACNNNNGTAYC 4 cut(s) 471, 483, 504, 516
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 869, 901
BauI CACGAG 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 261, 327
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 1 cut(s) 739
BccI CCATC 8 cut(s) 68, 125, 431, 431, 605, 635, 908, 938
BceAI ACGGC 1 cut(s) 72
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BcoDI GTCTC 2 cut(s) 269, 607
BfaI CTAG 1 cut(s) 979
BisI GCNGC 1 cut(s) 753
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 754
BmeRI GACNNNNNGTC 1 cut(s) 148
BplI GAGNNNNNCTC 2 cut(s) 547, 579
BpmI CTGGAG 2 cut(s) 524, 925
Bpu10I CCTNAGC 1 cut(s) 554
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 3 cut(s) 713, 806, 809
BsaJI CCNNGG 2 cut(s) 6, 279
BsaWI WCCGGW 1 cut(s) 239
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 268, 633, 877
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 4 cut(s) 627, 746, 868, 908
BseAI TCCGGA 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 6, 279
BseGI GGATG 1 cut(s) 120
BseLI CCNNNNNNNGG 4 cut(s) 246, 268, 633, 877
BseMII CTCAG 4 cut(s) 179, 545, 750, 822
BseNI ACTGG 4 cut(s) 627, 746, 868, 908
BsePI GCGCGC 1 cut(s) 34
BseXI GCAGC 1 cut(s) 739
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 2 cut(s) 874, 930
Bsh1236I CGCG 2 cut(s) 36, 106
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 5, 59
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 261, 327
BsiSI CCGG 2 cut(s) 240, 352
BsiWI CGTACG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 24, 263
BslI CCNNNNNNNGG 4 cut(s) 246, 268, 633, 877
BsmAI GTCTC 2 cut(s) 269, 607
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 24, 263
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 2 cut(s) 5, 59
Bsp1286I GDGCHC 2 cut(s) 261, 327
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 6 cut(s) 72, 271, 357, 376, 781, 820
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 1 cut(s) 698
BspANI GGCC 2 cut(s) 5, 59
BspCNI CTCAG 4 cut(s) 178, 546, 751, 823
BspEI TCCGGA 1 cut(s) 239
BspFNI CGCG 2 cut(s) 36, 106
BspPI GGATC 2 cut(s) 279, 789
BsrFI RCCGGY 1 cut(s) 351
BsrI ACTGG 4 cut(s) 627, 746, 868, 908
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 2 cut(s) 6, 279
BssHII GCGCGC 1 cut(s) 34
BssMI GATC 6 cut(s) 72, 271, 357, 376, 781, 820
BssNAI GTATAC 1 cut(s) 420
BssSI CACGAG 1 cut(s) 372
Bst1107I GTATAC 1 cut(s) 420
Bst2BI CACGAG 1 cut(s) 372
Bst4CI ACNGT 6 cut(s) 158, 224, 254, 523, 548, 595
BstC8I GCNNGC 1 cut(s) 36
BstDEI CTNAG 4 cut(s) 165, 554, 759, 831
BstDSI CCRYGG 1 cut(s) 279
BstEII GGTNACC 2 cut(s) 581, 866
BstF5I GGATG 1 cut(s) 120
BstFNI CGCG 2 cut(s) 36, 106
BstHHI GCGC 3 cut(s) 36, 38, 626
BstKTI GATC 6 cut(s) 75, 274, 360, 379, 784, 823
BstMAI GTCTC 2 cut(s) 269, 607
BstMBI GATC 6 cut(s) 72, 271, 357, 376, 781, 820
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 2 cut(s) 33, 688
BstPI GGTNACC 2 cut(s) 581, 866
BstUI CGCG 2 cut(s) 36, 106
BstV1I GCAGC 1 cut(s) 739
BstX2I RGATCY 2 cut(s) 271, 781
BstYI RGATCY 2 cut(s) 271, 781
BstZ17I GTATAC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 5, 59
BtgI CCRYGG 1 cut(s) 279
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 120
BtsIMutI CAGTG 4 cut(s) 591, 634, 937, 939
Cac8I GCNNGC 1 cut(s) 36
CaiI CAGNNNCTG 2 cut(s) 741, 960
CfoI GCGC 3 cut(s) 36, 38, 626
Cfr10I RCCGGY 1 cut(s) 351
CseI GACGC 1 cut(s) 90
Csp6I GTAC 2 cut(s) 381, 488
CviQI GTAC 2 cut(s) 381, 488
DdeI CTNAG 4 cut(s) 165, 554, 759, 831
DpnI GATC 6 cut(s) 74, 273, 359, 378, 783, 822
DpnII GATC 6 cut(s) 72, 271, 357, 376, 781, 820
DraIII CACNNNGTG 2 cut(s) 196, 407
DrdI GACNNNNNNGTC 1 cut(s) 92
DriI GACNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 3, 57
Eam1105I GACNNNNNGTC 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 259
Eco24I GRGCYC 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 3 cut(s) 450, 690, 709
Eco91I GGTNACC 2 cut(s) 581, 866
EcoICRI GAGCTC 1 cut(s) 259
EcoO65I GGTNACC 2 cut(s) 581, 866
EcoT38I GRGCYC 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 269
FaiI YATR 4 cut(s) 32, 312, 420, 622
FaqI GGGAC 2 cut(s) 24, 263
FauI CCCGC 1 cut(s) 691
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 1 cut(s) 753
FokI GGATG 1 cut(s) 107
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 1 cut(s) 753
FspBI CTAG 1 cut(s) 979
GlaI GCGC 3 cut(s) 35, 37, 625
GluI GCNGC 1 cut(s) 753
GsaI CCCAGC 1 cut(s) 250
GsuI CTGGAG 2 cut(s) 524, 925
HaeIII GGCC 2 cut(s) 5, 59
HapII CCGG 2 cut(s) 240, 352
HgaI GACGC 1 cut(s) 90
HhaI GCGC 3 cut(s) 36, 38, 626
Hin6I GCGC 3 cut(s) 34, 36, 624
HinP1I GCGC 3 cut(s) 34, 36, 624
HindIII AAGCTT 3 cut(s) 389, 680, 895
HinfI GANTC 3 cut(s) 12, 300, 535
HpaII CCGG 2 cut(s) 240, 352
HphI GGTGA 3 cut(s) 266, 419, 446
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 4 cut(s) 85, 220, 430, 832
Hpy188III TCNNGA 5 cut(s) 127, 240, 374, 541, 785
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 2 cut(s) 106, 300
HpyAV CCTTC 6 cut(s) 16, 425, 665, 808, 842, 871
HpyCH4III ACNGT 6 cut(s) 158, 224, 254, 523, 548, 595
HpyCH4V TGCA 4 cut(s) 233, 335, 891, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 688
HpyF3I CTNAG 4 cut(s) 165, 554, 759, 831
HspAI GCGC 3 cut(s) 34, 36, 624
Kpn2I TCCGGA 1 cut(s) 239
Kzo9I GATC 6 cut(s) 72, 271, 357, 376, 781, 820
LmnI GCTCC 1 cut(s) 264
Lsp1109I GCAGC 1 cut(s) 739
MaeI CTAG 1 cut(s) 979
MalI GATC 6 cut(s) 74, 273, 359, 378, 783, 822
MboI GATC 6 cut(s) 72, 271, 357, 376, 781, 820
MboII GAAGA 3 cut(s) 212, 853, 886
MflI RGATCY 2 cut(s) 271, 781
MhlI GDGCHC 2 cut(s) 261, 327
MluCI AATT 1 cut(s) 79
MlyI GAGTC 1 cut(s) 6
MmeI TCCRAC 2 cut(s) 108, 838
MnlI CCTC 6 cut(s) 134, 549, 596, 688, 799, 826
MroI TCCGGA 1 cut(s) 239
MseI TTAA 1 cut(s) 387
MspI CCGG 2 cut(s) 240, 352
Mva1269I GAATGC 1 cut(s) 335
MvnI CGCG 2 cut(s) 36, 106
MwoI GCNNNNNNNGC 2 cut(s) 33, 688
NdeII GATC 6 cut(s) 72, 271, 357, 376, 781, 820
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 6 cut(s) 52, 94, 107, 182, 440, 572
PauI GCGCGC 1 cut(s) 34
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 335
PfeI GAWTC 2 cut(s) 300, 535
Pfl23II CGTACG 1 cut(s) 380
PflFI GACNNNGTC 1 cut(s) 50
PkrI GCNGC 1 cut(s) 754
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 261
PspEI GGTNACC 2 cut(s) 581, 866
PspFI CCCAGC 1 cut(s) 246
PspLI CGTACG 1 cut(s) 380
PstNI CAGNNNCTG 2 cut(s) 741, 960
PsuI RGATCY 2 cut(s) 271, 781
PsyI GACNNNGTC 1 cut(s) 50
PteI GCGCGC 1 cut(s) 34
PvuI CGATCG 1 cut(s) 75
RsaI GTAC 2 cut(s) 382, 489
RsaNI GTAC 2 cut(s) 381, 488
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 1 cut(s) 753
Sau3AI GATC 6 cut(s) 72, 271, 357, 376, 781, 820
SchI GAGTC 1 cut(s) 6
SduI GDGCHC 2 cut(s) 261, 327
SmlI CTYRAG 3 cut(s) 692, 785, 824
SmoI CTYRAG 3 cut(s) 692, 785, 824
Sse9I AATT 1 cut(s) 79
SsiI CCGC 1 cut(s) 698
SspMI CTAG 1 cut(s) 979
SstI GAGCTC 1 cut(s) 261
TaaI ACNGT 6 cut(s) 158, 224, 254, 523, 548, 595
TaqI TCGA 3 cut(s) 75, 298, 704
TasI AATT 1 cut(s) 79
TfiI GAWTC 2 cut(s) 300, 535
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TscAI CASTG 4 cut(s) 598, 634, 937, 946
TseFI GTSAC 6 cut(s) 52, 94, 107, 182, 440, 572
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 6 cut(s) 52, 94, 107, 182, 440, 572
TspDTI ATGAA 1 cut(s) 861
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 4 cut(s) 598, 634, 937, 946
Tth111I GACNNNGTC 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 419
XspI CTAG 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.