pycom14g19480

Guanine nucleotide-binding protein subunit beta-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010922
Physical Location & Seq
Forward (+)
20783642 .. 20785597
1956 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g19480.2

Sequence Viewer

Length: 981 bp
ATGGGTGAGAATCTCGTTCTCCGCGGCACCATGAGGGCACATACCGACATGGTGACGGCCATCGCCATCCCAATCGACAACTCCGAGATGATCGTCAGCGCCTCCCGCGACAAGTCCATCATCCTCTGGCACCTGAACAAGGACGAGAAGACCTACGGTGTCCCCCGCCGCAGGCTCACCGGACATTCCCACTTCGTCGAGGATGTTGTCCTCTCCTCCGACGGCCAGTTCGCGCTGTCTGGATCCTGGGACGGCGAGCTCCGCCTCTGGGACTTGGCCCTCGGCGTTTCTGCTCGCCGCTTTGTGGGTCACACCAAGGATGTGCTCTCCGTGGCTTTCTCGATTGATAACCGTCAGATCGTATCGGCTTCCCGTGACCGCACGATCAAGCTCTGGAACACTCTCGGTGAGTGCAAGTTCACCATCCAGGACCAGGATGGGCACGGCGATTGGGTCAGCTGCGTCAGGTTCAGCCCCAGTACAATGCAGCCTACCATTGTATCCGCCTCGTGGGACAAGACTGTGAAAGTTTGGAACTTGTCTAACTGCAAGCTGAGGAACACTCTTGAGGGCCACAATGGGTATGTGAACACCGTTGCAGTGTCACCTGATGGTTCGTTGTGCGCCAGCGGAGGCAAAGACGGAGTAATTCTGCTCTGGGATTTGGCAGAGGGCAAGAGGCTGTATCATCTCGAGGCCGGCTCCATCATCCACGCTCTCTGCTTCAGTCCCAACAGGTACTGGCTGTGTGCCGCAACCGAGCAGAGCATCAAGATTTGGGATTTGGAGAGCAAGTCCATTGTGGAGGAGTTGAAGGTCGATCTCAAGACCGAGGCTGAGAAGACTGAGGATACCCATGCTGCTACTTCATACAAGAAGAAGGTTATATACTGTACAAGTTTGAACTGGAGCGCTGATGGCAGTACCTTGTTCAGTGGCTACACTGACGGAGTTATCAGAGTCTGGGGTATCGGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003735 GO:0005078 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006417 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010608 GO:0010646 GO:0010647 GO:0016020 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022613 GO:0022622 GO:0022626 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0032947 GO:0032991 GO:0033993 GO:0034248 GO:0035591 GO:0042221 GO:0042254 GO:0042325 GO:0042327 GO:0042788 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043408 GO:0043410 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045937 GO:0048364 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051704 GO:0051707 GO:0051716 GO:0060090 GO:0060255 GO:0060263 GO:0060267 GO:0065007 GO:0070013 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1990904 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko05162 map05162
Pfam Domains
Protein Families
WD40

Protein Analysis

327

Amino Acids

36.25

Weight (kDa)

6.56

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 26, 129
AccII CGCG 3 cut(s) 24, 108, 233
AclWI GGATC 2 cut(s) 237, 250
AcoI YGGCCR 3 cut(s) 57, 223, 973
AcuI CTGAAG 1 cut(s) 709
AfaI GTAC 4 cut(s) 481, 740, 895, 925
AfeI AGCGCT 1 cut(s) 913
AfiI CCNNNNNNNGG 6 cut(s) 139, 171, 268, 304, 433, 510
AgsI TTSAA 2 cut(s) 814, 904
AjnI CCWGG 3 cut(s) 245, 426, 432
AluBI AGCT 4 cut(s) 259, 391, 459, 553
AluI AGCT 4 cut(s) 259, 391, 459, 553
Alw21I GWGCWC 2 cut(s) 261, 327
AlwI GGATC 2 cut(s) 237, 250
AlwNI CAGNNNCTG 2 cut(s) 741, 963
Ama87I CYCGRG 1 cut(s) 692
Aor51HI AGCGCT 1 cut(s) 913
AoxI GGCC 6 cut(s) 57, 223, 276, 571, 696, 973
ApeKI GCWGC 3 cut(s) 459, 487, 860
ArsI GACNNNNNNTTYG 2 cut(s) 212, 244
AspLEI GCGC 4 cut(s) 101, 235, 626, 914
AspS9I GGNCC 3 cut(s) 277, 430, 571
AsuHPI GGTGA 6 cut(s) 17, 64, 169, 412, 419, 597
AvaI CYCGRG 1 cut(s) 692
AvaII GGWCC 1 cut(s) 430
BaeGI GKGCMC 2 cut(s) 40, 444
BaeI ACNNNNGTAYC 2 cut(s) 483, 516
BamHI GGATCC 1 cut(s) 242
BanI GGYRCC 2 cut(s) 26, 129
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 872, 904
BauI CACGAG 1 cut(s) 508
BbsI GAAGAC 2 cut(s) 155, 848
Bbv12I GWGCWC 2 cut(s) 261, 327
BbvCI CCTCAGC 1 cut(s) 554
BbvI GCAGC 3 cut(s) 446, 499, 847
BccI CCATC 8 cut(s) 68, 74, 125, 431, 431, 605, 713, 911
BceAI ACGGC 5 cut(s) 72, 238, 268, 460, 960
BciT130I CCWGG 3 cut(s) 247, 428, 434
BciVI GTATCC 2 cut(s) 511, 844
BfoI RGCGCY 2 cut(s) 102, 915
BfuI GTATCC 2 cut(s) 511, 844
BisI GCNGC 7 cut(s) 25, 169, 298, 460, 488, 753, 861
BlsI GCNGC 7 cut(s) 26, 170, 299, 461, 489, 754, 862
Bme1390I CCNGG 3 cut(s) 247, 428, 434
Bme18I GGWCC 1 cut(s) 430
BmeT110I CYCGRG 1 cut(s) 692
BmgT120I GGNCC 3 cut(s) 277, 430, 571
BmiI GGNNCC 4 cut(s) 28, 131, 244, 703
BmrFI CCNGG 3 cut(s) 247, 428, 434
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 1 cut(s) 777
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 2 cut(s) 155, 848
BplI GAGNNNNNCTC 4 cut(s) 547, 579, 686, 718
BpmI CTGGAG 1 cut(s) 928
Bpu10I CCTNAGC 1 cut(s) 554
BpuEI CTTGAG 2 cut(s) 587, 809
BsaJI CCNNGG 6 cut(s) 22, 246, 280, 315, 330, 831
BsaWI WCCGGW 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 779, 809
Bsc4I CCNNNNNNNGG 6 cut(s) 139, 171, 268, 304, 433, 510
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 4 cut(s) 226, 477, 746, 911
BseBI CCWGG 3 cut(s) 247, 428, 434
BseDI CCNNGG 6 cut(s) 22, 246, 280, 315, 330, 831
BseGI GGATG 7 cut(s) 66, 120, 208, 325, 423, 442, 708
BseLI CCNNNNNNNGG 6 cut(s) 139, 171, 268, 304, 433, 510
BseMII CTCAG 3 cut(s) 545, 828, 837
BseNI ACTGG 4 cut(s) 226, 477, 746, 911
BseRI GAGGAG 2 cut(s) 205, 821
BseSI GKGCMC 2 cut(s) 40, 444
BseX3I CGGCCG 1 cut(s) 973
BseXI GCAGC 3 cut(s) 446, 499, 847
Bsh1236I CGCG 3 cut(s) 24, 108, 233
Bsh1285I CGRYCG 1 cut(s) 976
BshFI GGCC 6 cut(s) 59, 225, 278, 573, 698, 975
BshNI GGYRCC 2 cut(s) 26, 129
BsiEI CGRYCG 1 cut(s) 976
BsiHKAI GWGCWC 2 cut(s) 261, 327
BsiHKCI CYCGRG 1 cut(s) 692
BsiSI CCGG 2 cut(s) 180, 699
BslFI GGGAC 5 cut(s) 146, 263, 284, 527, 714
BslI CCNNNNNNNGG 6 cut(s) 139, 171, 268, 304, 433, 510
BsmFI GGGAC 5 cut(s) 146, 263, 284, 527, 714
BsnI GGCC 6 cut(s) 59, 225, 278, 573, 698, 975
BsoBI CYCGRG 1 cut(s) 692
Bsp1286I GDGCHC 4 cut(s) 40, 261, 327, 444
Bsp1407I TGTACA 1 cut(s) 893
Bsp143I GATC 5 cut(s) 90, 242, 357, 384, 820
BspANI GGCC 6 cut(s) 59, 225, 278, 573, 698, 975
BspCNI CTCAG 3 cut(s) 546, 829, 838
BspFNI CGCG 3 cut(s) 24, 108, 233
BspLI GGNNCC 4 cut(s) 28, 131, 244, 703
BspPI GGATC 2 cut(s) 237, 250
BspT107I GGYRCC 2 cut(s) 26, 129
BsrFI RCCGGY 1 cut(s) 698
BsrGI TGTACA 1 cut(s) 893
BsrI ACTGG 4 cut(s) 226, 477, 746, 911
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 6 cut(s) 22, 246, 280, 315, 330, 831
BssMI GATC 5 cut(s) 90, 242, 357, 384, 820
BssSI CACGAG 1 cut(s) 508
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 508
Bst2UI CCWGG 3 cut(s) 247, 428, 434
Bst4CI ACNGT 5 cut(s) 158, 353, 523, 595, 893
BstAUI TGTACA 1 cut(s) 893
BstC8I GCNNGC 6 cut(s) 173, 257, 295, 551, 628, 700
BstDEI CTNAG 3 cut(s) 554, 837, 846
BstDSI CCRYGG 2 cut(s) 22, 330
BstENI CCTNNNNNAGG 1 cut(s) 137
BstF5I GGATG 7 cut(s) 66, 120, 208, 325, 423, 442, 708
BstFNI CGCG 3 cut(s) 24, 108, 233
BstH2I RGCGCY 2 cut(s) 102, 915
BstHHI GCGC 4 cut(s) 101, 235, 626, 914
BstKTI GATC 5 cut(s) 93, 245, 360, 387, 823
BstMBI GATC 5 cut(s) 90, 242, 357, 384, 820
BstMCI CGRYCG 1 cut(s) 976
BstMWI GCNNNNNNNGC 3 cut(s) 105, 261, 918
BstNI CCWGG 3 cut(s) 247, 428, 434
BstSCI CCNGG 3 cut(s) 245, 426, 432
BstSLI GKGCMC 2 cut(s) 40, 444
BstUI CGCG 3 cut(s) 24, 108, 233
BstV1I GCAGC 3 cut(s) 446, 499, 847
BstV2I GAAGAC 2 cut(s) 155, 848
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BstZI CGGCCG 1 cut(s) 973
BsuI GTATCC 2 cut(s) 511, 844
BsuRI GGCC 6 cut(s) 59, 225, 278, 573, 698, 975
BtgI CCRYGG 2 cut(s) 22, 330
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 7 cut(s) 66, 120, 208, 325, 423, 442, 708
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 3 cut(s) 606, 940, 942
Cac8I GCNNGC 6 cut(s) 173, 257, 295, 551, 628, 700
CaiI CAGNNNCTG 2 cut(s) 741, 963
CfoI GCGC 4 cut(s) 101, 235, 626, 914
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 3 cut(s) 277, 430, 571
Cfr42I CCGCGG 1 cut(s) 25
CseI GACGC 1 cut(s) 451
Csp6I GTAC 4 cut(s) 480, 739, 894, 924
CviAII CATG 3 cut(s) 31, 49, 857
CviQI GTAC 4 cut(s) 480, 739, 894, 924
DdeI CTNAG 3 cut(s) 554, 837, 846
DpnI GATC 5 cut(s) 92, 244, 359, 386, 822
DpnII GATC 5 cut(s) 90, 242, 357, 384, 820
EaeI YGGCCR 3 cut(s) 57, 223, 973
EagI CGGCCG 1 cut(s) 973
EciI GGCGGA 2 cut(s) 251, 493
Ecl136II GAGCTC 1 cut(s) 259
EclXI CGGCCG 1 cut(s) 973
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 1 cut(s) 261
Eco47I GGWCC 1 cut(s) 430
Eco47III AGCGCT 1 cut(s) 913
Eco52I CGGCCG 1 cut(s) 973
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 709
Eco88I CYCGRG 1 cut(s) 692
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 137
EcoRII CCWGG 3 cut(s) 245, 426, 432
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 3 cut(s) 34, 52, 860
FaiI YATR 8 cut(s) 32, 42, 50, 585, 858, 871, 887, 889
FaqI GGGAC 5 cut(s) 146, 263, 284, 527, 714
FatI CATG 3 cut(s) 30, 48, 856
FauI CCCGC 2 cut(s) 113, 173
Fnu4HI GCNGC 7 cut(s) 25, 169, 298, 460, 488, 753, 861
FokI GGATG 7 cut(s) 53, 107, 215, 332, 410, 449, 695
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 7 cut(s) 25, 169, 298, 460, 488, 753, 861
GlaI GCGC 4 cut(s) 100, 234, 625, 913
GluI GCNGC 7 cut(s) 25, 169, 298, 460, 488, 753, 861
GsuI CTGGAG 1 cut(s) 928
HaeII RGCGCY 2 cut(s) 102, 915
HaeIII GGCC 6 cut(s) 59, 225, 278, 573, 698, 975
HapII CCGG 2 cut(s) 180, 699
HgaI GACGC 1 cut(s) 451
HhaI GCGC 4 cut(s) 101, 235, 626, 914
Hin1II CATG 3 cut(s) 34, 52, 860
Hin6I GCGC 4 cut(s) 99, 233, 624, 912
HinP1I GCGC 4 cut(s) 99, 233, 624, 912
HinfI GANTC 2 cut(s) 10, 960
HpaII CCGG 2 cut(s) 180, 699
HphI GGTGA 6 cut(s) 17, 64, 169, 412, 419, 597
Hpy166II GTNNAC 2 cut(s) 420, 589
Hpy188I TCNGA 4 cut(s) 85, 220, 357, 959
Hpy188III TCNNGA 7 cut(s) 240, 340, 394, 566, 692, 772, 826
Hpy8I GTNNAC 2 cut(s) 420, 589
Hpy99I CGWCG 2 cut(s) 200, 224
HpyAV CCTTC 2 cut(s) 808, 874
HpyCH4III ACNGT 5 cut(s) 158, 353, 523, 595, 893
HpyCH4V TGCA 4 cut(s) 414, 487, 549, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 261, 918
HpyF3I CTNAG 3 cut(s) 554, 837, 846
Hsp92II CATG 3 cut(s) 34, 52, 860
HspAI GCGC 4 cut(s) 99, 233, 624, 912
KroI GCCGGC 1 cut(s) 698
KroNI GCCGGC 1 cut(s) 700
KspI CCGCGG 1 cut(s) 25
Kzo9I GATC 5 cut(s) 90, 242, 357, 384, 820
LmnI GCTCC 3 cut(s) 264, 707, 909
Lsp1109I GCAGC 3 cut(s) 446, 499, 847
LweI GCATC 1 cut(s) 777
MaeIII GTNAC 4 cut(s) 52, 308, 374, 603
MalI GATC 5 cut(s) 92, 244, 359, 386, 822
MboI GATC 5 cut(s) 90, 242, 357, 384, 820
MboII GAAGA 3 cut(s) 160, 853, 889
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 4 cut(s) 40, 261, 327, 444
MluCI AATT 1 cut(s) 648
MlyI GAGTC 1 cut(s) 969
MmeI TCCRAC 1 cut(s) 243
MroNI GCCGGC 1 cut(s) 698
MspA1I CMGCKG 3 cut(s) 24, 459, 630
MspI CCGG 2 cut(s) 180, 699
MspR9I CCNGG 3 cut(s) 247, 428, 434
MvaI CCWGG 3 cut(s) 247, 428, 434
MvnI CGCG 3 cut(s) 24, 108, 233
MwoI GCNNNNNNNGC 3 cut(s) 105, 261, 918
NaeI GCCGGC 1 cut(s) 700
NdeII GATC 5 cut(s) 90, 242, 357, 384, 820
NgoMIV GCCGGC 1 cut(s) 698
NlaIII CATG 3 cut(s) 34, 52, 860
NlaIV GGNNCC 4 cut(s) 28, 131, 244, 703
NmeAIII GCCGAG 1 cut(s) 261
NmuCI GTSAC 4 cut(s) 52, 308, 374, 603
PaeR7I CTCGAG 1 cut(s) 692
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 700
PfeI GAWTC 1 cut(s) 10
PfoI TCCNGGA 1 cut(s) 426
PkrI GCNGC 7 cut(s) 26, 170, 299, 461, 489, 754, 862
PleI GAGTC 1 cut(s) 968
PpsI GAGTC 1 cut(s) 968
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 245, 426, 432
PspGI CCWGG 3 cut(s) 245, 426, 432
PspN4I GGNNCC 4 cut(s) 28, 131, 244, 703
PspPI GGNCC 3 cut(s) 277, 430, 571
PstNI CAGNNNCTG 2 cut(s) 741, 963
PsuI RGATCY 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 459
RsaI GTAC 4 cut(s) 481, 740, 895, 925
RsaNI GTAC 4 cut(s) 480, 739, 894, 924
SacI GAGCTC 1 cut(s) 261
SacII CCGCGG 1 cut(s) 25
SatI GCNGC 7 cut(s) 25, 169, 298, 460, 488, 753, 861
Sau3AI GATC 5 cut(s) 90, 242, 357, 384, 820
Sau96I GGNCC 3 cut(s) 277, 430, 571
SchI GAGTC 1 cut(s) 969
ScrFI CCNGG 3 cut(s) 247, 428, 434
SduI GDGCHC 4 cut(s) 40, 261, 327, 444
SfaNI GCATC 1 cut(s) 777
Sfr274I CTCGAG 1 cut(s) 692
Sfr303I CCGCGG 1 cut(s) 25
SgrBI CCGCGG 1 cut(s) 25
SinI GGWCC 1 cut(s) 430
SlaI CTCGAG 1 cut(s) 692
SmlI CTYRAG 3 cut(s) 566, 692, 824
SmoI CTYRAG 3 cut(s) 566, 692, 824
Sse9I AATT 1 cut(s) 648
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 245, 426, 432
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 5 cut(s) 158, 353, 523, 595, 893
TaqI TCGA 5 cut(s) 75, 198, 341, 693, 819
TaqII GACCGA 1 cut(s) 845
TasI AATT 1 cut(s) 648
TatI WGTACW 2 cut(s) 479, 893
TauI GCSGC 4 cut(s) 27, 171, 300, 755
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 3 cut(s) 606, 940, 949
TseFI GTSAC 4 cut(s) 52, 308, 374, 603
TseI GCWGC 3 cut(s) 459, 487, 860
Tsp45I GTSAC 4 cut(s) 52, 308, 374, 603
TspDTI ATGAA 1 cut(s) 858
TspGWI ACGGA 3 cut(s) 319, 657, 963
TspRI CASTG 3 cut(s) 606, 940, 949
VpaK11BI GGWCC 1 cut(s) 430
XagI CCTNNNNNAGG 1 cut(s) 137
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.