Rorug06G0419700

Guanine nucleotide-binding protein subunit beta-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010922
Physical Location & Seq
Forward (+)
55858160 .. 55860524
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0419700.1

Sequence Viewer

Length: 717 bp
ATGGAAGGGCATGGACTTACAAGTCTTGTAGTATTGGGAGTTATTTCATGGACAACAGCATTTCTATTCATCAGAAAAATGTGTCCAAAGAGATCCTTTGGATTCTGCAACCGCCTTGTTTCGACAGTCCATGCAACCATTGCTGTAACCCTAGCTTCACTCTCTGTCGAAGACTGGAGATGCCCAGTTTGTCCTCTGGCTGCTCAATCTTCTCCTCAGCAGATGAAAGCACTAGCAGTGAGCCTTTCTTATCTCATCTATGATCTCATCTGTTGCCTTTTTGACAAGCAATTCAGTCTTGACAATTTCTTCCACCATTTGGTTAGCATTATTGGAATTGTAGCAGGCCTTGCATACCAAAGGTTATGTGGGTCAGAGATGGTAGCAGCATTGTGGATAACAGAGGTCTCTAGCCCTTTCCTCCACTTGAGGGAGATTCTCAAAGAACTTGGTTACAAGGACACCGACCTAAACTTAGCAGCAGATATTTCATTTGCAGCTATATTCTCGGTTGCAAGGATGGGAGGCGGACCTTATCTCGCTTATGTAACACTATCTGCTCACAATCCTTTCATCATTAAGGGAATGGCAGTTGGACTGCAGCTGGTCAGTACTTTCTGGTTCTACAAGATAGCGAGAATGGTGATGTACAAATTAACCAAGAGGACGACTAGTGCAACGAAGGAGGCTGACATCAACCATAAAAGAGAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003735 GO:0005078 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0005886 GO:0006417 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009889 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010608 GO:0010646 GO:0010647 GO:0016020 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0022613 GO:0022622 GO:0022626 GO:0023051 GO:0023056 GO:0031323 GO:0031325 GO:0031326 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032870 GO:0032947 GO:0032991 GO:0033993 GO:0034248 GO:0035591 GO:0042221 GO:0042254 GO:0042325 GO:0042327 GO:0042788 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043408 GO:0043410 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045937 GO:0048364 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051704 GO:0051707 GO:0051716 GO:0060090 GO:0060255 GO:0060263 GO:0060267 GO:0065007 GO:0070013 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071840 GO:0071944 GO:0080090 GO:0090351 GO:0097305 GO:0097306 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:1990904 GO:2000112
KEGG Pathways
Metabolic & Signaling
ko05162 map05162
Pfam Domains
Protein Families
WD40

Protein Analysis

238

Amino Acids

26.46

Weight (kDa)

8.96

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 35 - 213 2.4e-24 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 21
AccB7I CCANNNNNTGG 1 cut(s) 319
AciI CCGC 2 cut(s) 112, 528
AclWI GGATC 1 cut(s) 87
AfaI GTAC 2 cut(s) 613, 650
AfiI CCNNNNNNNGG 2 cut(s) 319, 430
AhlI ACTAGT 1 cut(s) 671
AluBI AGCT 3 cut(s) 155, 500, 604
AluI AGCT 3 cut(s) 155, 500, 604
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 1 cut(s) 87
AoxI GGCC 1 cut(s) 346
ApeKI GCWGC 5 cut(s) 200, 386, 479, 497, 601
AspS9I GGNCC 1 cut(s) 530
AsuHPI GGTGA 1 cut(s) 655
AvaII GGWCC 1 cut(s) 530
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 5 cut(s) 187, 398, 491, 509, 613
BccI CCATC 2 cut(s) 373, 514
BcoDI GTCTC 1 cut(s) 412
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 4 cut(s) 152, 233, 411, 672
BfmI CTRYAG 1 cut(s) 599
BisI GCNGC 5 cut(s) 201, 387, 480, 498, 602
BlsI GCNGC 5 cut(s) 202, 388, 481, 499, 603
BmcAI AGTACT 1 cut(s) 613
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 1 cut(s) 530
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 1 cut(s) 170
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 1 cut(s) 216
BpuEI CTTGAG 1 cut(s) 448
BsaI GGTCTC 1 cut(s) 412
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 430
Bse1I ACTGG 2 cut(s) 179, 185
Bse3DI GCAATG 1 cut(s) 138
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 319, 430
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 179, 185
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 5 cut(s) 187, 398, 491, 509, 613
BshFI GGCC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 319, 430
BsmAI GTCTC 1 cut(s) 412
BsnI GGCC 1 cut(s) 348
Bso31I GGTCTC 1 cut(s) 412
Bsp1407I TGTACA 1 cut(s) 648
Bsp143I GATC 2 cut(s) 92, 262
BspACI CCGC 2 cut(s) 112, 528
BspANI GGCC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 603
BspPI GGATC 1 cut(s) 87
BspTNI GGTCTC 1 cut(s) 412
BsrDI GCAATG 1 cut(s) 138
BsrGI TGTACA 1 cut(s) 648
BsrI ACTGG 2 cut(s) 179, 185
BssMI GATC 2 cut(s) 92, 262
Bst4CI ACNGT 1 cut(s) 127
BstAPI GCANNNNNTGC 2 cut(s) 140, 350
BstAUI TGTACA 1 cut(s) 648
BstC8I GCNNGC 1 cut(s) 346
BstDEI CTNAG 2 cut(s) 216, 475
BstF5I GGATG 1 cut(s) 525
BstKTI GATC 2 cut(s) 95, 265
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 2 cut(s) 92, 262
BstMWI GCNNNNNNNGC 2 cut(s) 140, 350
BstSFI CTRYAG 1 cut(s) 599
BstV1I GCAGC 5 cut(s) 187, 398, 491, 509, 613
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 92
BstYI RGATCY 1 cut(s) 92
BsuRI GGCC 1 cut(s) 348
BtsCI GGATG 1 cut(s) 525
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 612, 649
CviAII CATG 3 cut(s) 11, 48, 131
CviJI RGCY 8 cut(s) 155, 200, 243, 348, 414, 500, 604, 689
CviKI_1 RGCY 8 cut(s) 155, 200, 243, 348, 414, 500, 604, 689
CviQI GTAC 2 cut(s) 612, 649
DdeI CTNAG 2 cut(s) 216, 475
DpnI GATC 2 cut(s) 94, 264
DpnII GATC 2 cut(s) 92, 262
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 543
Eco147I AGGCCT 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 412
Eco47I GGWCC 1 cut(s) 530
FaeI CATG 3 cut(s) 14, 51, 134
FaiI YATR 9 cut(s) 12, 49, 132, 261, 355, 367, 503, 546, 702
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FatI CATG 3 cut(s) 10, 47, 130
Fnu4HI GCNGC 5 cut(s) 201, 387, 480, 498, 602
FokI GGATG 1 cut(s) 532
Fsp4HI GCNGC 5 cut(s) 201, 387, 480, 498, 602
FspBI CTAG 4 cut(s) 152, 233, 411, 672
GluI GCNGC 5 cut(s) 201, 387, 480, 498, 602
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 1 cut(s) 348
Hin1II CATG 3 cut(s) 14, 51, 134
HinfI GANTC 2 cut(s) 102, 436
HphI GGTGA 1 cut(s) 655
Hpy188I TCNGA 2 cut(s) 74, 376
Hpy188III TCNNGA 1 cut(s) 299
HpyAV CCTTC 1 cut(s) 676
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4V TGCA 7 cut(s) 108, 134, 353, 497, 515, 601, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 350
HpyF3I CTNAG 2 cut(s) 216, 475
Hsp92II CATG 3 cut(s) 14, 51, 134
Kzo9I GATC 2 cut(s) 92, 262
LpnPI CCDG 6 cut(s) 160, 182, 198, 330, 590, 604
Lsp1109I GCAGC 5 cut(s) 187, 398, 491, 509, 613
LweI GCATC 1 cut(s) 170
MaeI CTAG 4 cut(s) 152, 233, 411, 672
MaeIII GTNAC 3 cut(s) 145, 452, 547
MalI GATC 2 cut(s) 94, 264
MboI GATC 2 cut(s) 92, 262
MboII GAAGA 3 cut(s) 182, 201, 301
MflI RGATCY 1 cut(s) 92
MluCI AATT 5 cut(s) 290, 304, 336, 653, 712
MmeI TCCRAC 1 cut(s) 574
MnlI CCTC 8 cut(s) 204, 225, 397, 423, 431, 518, 657, 679
MseI TTAA 2 cut(s) 579, 656
MspA1I CMGCKG 1 cut(s) 604
MwoI GCNNNNNNNGC 2 cut(s) 140, 350
NdeII GATC 2 cut(s) 92, 262
NlaIII CATG 3 cut(s) 14, 51, 134
PceI AGGCCT 1 cut(s) 348
PfeI GAWTC 2 cut(s) 102, 436
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 5 cut(s) 202, 388, 481, 499, 603
PspPI GGNCC 1 cut(s) 530
PstI CTGCAG 1 cut(s) 603
PsuI RGATCY 1 cut(s) 92
PvuII CAGCTG 1 cut(s) 604
RsaI GTAC 2 cut(s) 613, 650
RsaNI GTAC 2 cut(s) 612, 649
SaqAI TTAA 2 cut(s) 579, 656
SatI GCNGC 5 cut(s) 201, 387, 480, 498, 602
Sau3AI GATC 2 cut(s) 92, 262
Sau96I GGNCC 1 cut(s) 530
ScaI AGTACT 1 cut(s) 613
SetI ASST 7 cut(s) 157, 365, 408, 471, 502, 535, 606
SfaNI GCATC 1 cut(s) 170
SfcI CTRYAG 1 cut(s) 599
SinI GGWCC 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
SpeI ACTAGT 1 cut(s) 671
Sse9I AATT 5 cut(s) 290, 304, 336, 653, 712
SseBI AGGCCT 1 cut(s) 348
SsiI CCGC 2 cut(s) 112, 528
SspMI CTAG 4 cut(s) 152, 233, 411, 672
StuI AGGCCT 1 cut(s) 348
TaaI ACNGT 1 cut(s) 127
TaqI TCGA 2 cut(s) 122, 168
TasI AATT 5 cut(s) 290, 304, 336, 653, 712
TatI WGTACW 2 cut(s) 611, 648
TfiI GAWTC 2 cut(s) 102, 436
Tru1I TTAA 2 cut(s) 579, 656
Tru9I TTAA 2 cut(s) 579, 656
TscAI CASTG 1 cut(s) 243
TseI GCWGC 5 cut(s) 200, 386, 479, 497, 601
TspDTI ATGAA 5 cut(s) 36, 58, 239, 480, 562
TspRI CASTG 1 cut(s) 243
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 530
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XspI CTAG 4 cut(s) 152, 233, 411, 672
ZrmI AGTACT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.