AT3G20480

tetraacyldisaccharide 4'-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003555
Physical Location & Seq
Reverse (-)
7151387 .. 7153464
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G20480.3

Sequence Viewer

Length: 1188 bp
ATGGAGAAGCTGAGGAAGGTGGTGAACGAAATCGCCTACACGCGAGTCCATACGAATTCCCCTGCTCTTCACCGTTCTCTCGTTCCCTTCCTAACAATCGCTTCGTCTCTATACGGAGTCGCTCTCCAAATCCGCCGCTCTCTCTACCGCTACTCACTCTTACAGAAACACCGATTACCCGTTCCGGTGATCAGCGTTGGGAATCTGAGCTGGGGAGGTAATGGGAAGACGCCAATGGTGGAATACATTTCTCAATTCCTCGTTGATTCTGGACTTACACCTCTTATTCTCACTCGTGGTTATGCTGGTGGAGATGAAGTCAAAATGCTTGAAAGACATCTTCGTGGTGGACCGGTAAAGATTGGCGTTGGGGCTAACCGGGCAGCTACTGCTGCTTTGTTTTTGGATAAGTATGGTTGTGTAGATTCTTCTAGTTTAAGGTCATTCTTTGATCTTCATGAAAGAGCACAAGTTTGGACCATTTCTGAAAAAATTGGTTGTATCATATTAGATGATGGGATGCAGCATTGGAGTTTGTCTCGTGATCTCGAGATTGTGATGCTTAACGGGCTGAATCCATGGGGAAATGGTCATTTGATGCCTCATGGTCCTTTACGAGAACCTCTTCTGGCGCTTGAACGGGCAGATGTTGCTGTAGTACACCATGTGGACTTGATTACTAAACAAAGCCTCAGAGATATTGAGAACATGATACAAGGATTCAAAAAGTCGATACCGATTTTCTACTCCAAGATGGTGCCCAAATACTTGTTCGATGTTAAAAATGCTAGGTCACATGTAGCTTTAGAAGCGTTGCGCTGTGCCTCTGTGCTATGTGTTTCCGCAATTGGATCCGCTGATGCATTTGTCAAGAGCATCGAGATGACTGGAGCACATTATGTTGATAGACTTGATTTCAGTGATCATCATTTATTTGAAGCAGAGGATGTTGAAACTATGAGTAGGAGAGCAAAGGGACTAGAACACAAATCTAATTGCAAGCCTATCATTGTGGTAACTGAAAAGGATTATGATAGAGACCCGGAGATCCTCAAATGTCTGGACTCATACACAGTCTTGGTTCTATGCTCTGAGTTGCAAATAACTCCTATTTTGGAAACTGATGTAGATAGCTTTAATTATACACTTATGAAAGCTCTAGCTGCTAAGTTCTATGTTTCCAGTTAG

Protein Analysis

395

Amino Acids

44.2

Weight (kDa)

7.68

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LpxK PF02606 2 - 242 2.2e-40 Tetraacyldisaccharide-1-P 4'-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 757
AccBSI CCGCTC 1 cut(s) 138
AccII CGCG 1 cut(s) 43
AciI CCGC 5 cut(s) 133, 136, 148, 843, 855
AclWI GGATC 3 cut(s) 846, 859, 1042
AcsI RAATTY 1 cut(s) 55
AcyI GRCGYC 1 cut(s) 230
AdeI CACNNNGTG 1 cut(s) 667
AfaI GTAC 1 cut(s) 660
AflIII ACRYGT 1 cut(s) 796
AgeI ACCGGT 1 cut(s) 352
AgsI TTSAA 5 cut(s) 332, 638, 724, 938, 953
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AluBI AGCT 7 cut(s) 10, 210, 386, 803, 1134, 1157, 1163
AluI AGCT 7 cut(s) 10, 210, 386, 803, 1134, 1157, 1163
Alw21I GWGCWC 2 cut(s) 469, 895
Alw26I GTCTC 3 cut(s) 111, 543, 1032
AlwI GGATC 3 cut(s) 846, 859, 1042
AlwNI CAGNNNCTG 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 548
ApeKI GCWGC 4 cut(s) 383, 392, 523, 1163
ApoI RAATTY 1 cut(s) 55
AsiGI ACCGGT 1 cut(s) 352
Asp700I GAANNNNTTC 1 cut(s) 624
AspLEI GCGC 2 cut(s) 634, 819
AspS9I GGNCC 3 cut(s) 350, 477, 608
AsuC2I CCSGG 2 cut(s) 380, 1043
AsuHPI GGTGA 3 cut(s) 34, 62, 199
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 3 cut(s) 350, 477, 608
BaeGI GKGCMC 1 cut(s) 762
BamHI GGATCC 1 cut(s) 851
BanI GGYRCC 1 cut(s) 757
BauI CACGAG 2 cut(s) 294, 540
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 2 cut(s) 469, 895
BbvCI CCTCAGC 1 cut(s) 11
BbvI GCAGC 4 cut(s) 379, 395, 535, 1150
BccI CCATC 2 cut(s) 509, 748
BclI TGATCA 2 cut(s) 189, 922
BcnI CCSGG 2 cut(s) 380, 1043
BcoDI GTCTC 3 cut(s) 111, 543, 1032
BfaI CTAG 4 cut(s) 432, 789, 980, 1160
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 635
BisI GCNGC 5 cut(s) 136, 384, 393, 524, 1164
BlsI GCNGC 5 cut(s) 137, 385, 394, 525, 1165
Bme1390I CCNGG 2 cut(s) 380, 1043
Bme18I GGWCC 3 cut(s) 350, 477, 608
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 3 cut(s) 350, 477, 608
BmiI GGNNCC 2 cut(s) 759, 853
BmrFI CCNGG 2 cut(s) 380, 1043
BmsI GCATC 5 cut(s) 510, 549, 588, 850, 885
BpiI GAAGAC 1 cut(s) 233
BplI GAGNNNNNCTC 4 cut(s) 108, 140, 523, 555
BpmI CTGGAG 1 cut(s) 909
Bpu10I CCTNAGC 1 cut(s) 11
BpuMI CCSGG 2 cut(s) 380, 1043
BsaHI GRCGYC 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 1032
BsaJI CCNNGG 1 cut(s) 578
BsaWI WCCGGW 2 cut(s) 184, 352
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bse118I RCCGGY 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 892, 1182
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 2 cut(s) 525, 952
BseMII CTCAG 3 cut(s) 197, 706, 1083
BseNI ACTGG 2 cut(s) 892, 1182
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 4 cut(s) 379, 395, 535, 1150
BseYI CCCAGC 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 43
BshNI GGYRCC 1 cut(s) 757
BshTI ACCGGT 1 cut(s) 352
BsiHKAI GWGCWC 2 cut(s) 469, 895
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 4 cut(s) 185, 353, 379, 1043
BslFI GGGAC 1 cut(s) 990
BsmAI GTCTC 3 cut(s) 111, 543, 1032
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 990
Bso31I GGTCTC 1 cut(s) 1032
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 3 cut(s) 469, 762, 895
Bsp143I GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 5 cut(s) 133, 136, 148, 843, 855
BspCNI CTCAG 3 cut(s) 198, 705, 1084
BspFNI CGCG 1 cut(s) 43
BspHI TCATGA 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 759, 853
BspPI GGATC 3 cut(s) 846, 859, 1042
BspQI GCTCTTC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 757
BspTNI GGTCTC 1 cut(s) 1032
BsrBI CCGCTC 1 cut(s) 138
BsrFI RCCGGY 1 cut(s) 352
BsrI ACTGG 2 cut(s) 892, 1182
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
BssNI GRCGYC 1 cut(s) 230
BssSI CACGAG 2 cut(s) 294, 540
BssT1I CCWWGG 1 cut(s) 578
Bst2BI CACGAG 2 cut(s) 294, 540
Bst4CI ACNGT 2 cut(s) 74, 1075
Bst6I CTCTTC 2 cut(s) 72, 630
BstACI GRCGYC 1 cut(s) 230
BstAPI GCANNNNNTGC 2 cut(s) 389, 650
BstC8I GCNNGC 1 cut(s) 1001
BstDEI CTNAG 5 cut(s) 11, 206, 692, 1092, 1167
BstDSI CCRYGG 1 cut(s) 578
BstF5I GGATG 2 cut(s) 525, 952
BstFNI CGCG 1 cut(s) 43
BstH2I RGCGCY 1 cut(s) 635
BstHHI GCGC 2 cut(s) 634, 819
BstKTI GATC 6 cut(s) 192, 454, 547, 854, 925, 1050
BstMAI GTCTC 3 cut(s) 111, 543, 1032
BstMBI GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
BstMWI GCNNNNNNNGC 7 cut(s) 380, 389, 392, 568, 650, 809, 1163
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 2 cut(s) 378, 1041
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 762
BstUI CGCG 1 cut(s) 43
BstV1I GCAGC 4 cut(s) 379, 395, 535, 1150
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 2 cut(s) 851, 1047
BstYI RGATCY 2 cut(s) 851, 1047
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 2 cut(s) 525, 952
BtsIMutI CAGTG 1 cut(s) 925
Cac8I GCNNGC 1 cut(s) 1001
CaiI CAGNNNCTG 1 cut(s) 389
CciI TCATGA 1 cut(s) 457
CfoI GCGC 2 cut(s) 634, 819
Cfr10I RCCGGY 1 cut(s) 352
Cfr13I GGNCC 3 cut(s) 350, 477, 608
CseI GACGC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 659
CspAI ACCGGT 1 cut(s) 352
CviAII CATG 6 cut(s) 458, 579, 605, 665, 709, 797
CviQI GTAC 1 cut(s) 659
DdeI CTNAG 5 cut(s) 11, 206, 692, 1092, 1167
DpnI GATC 6 cut(s) 191, 453, 546, 853, 924, 1049
DpnII GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
DraIII CACNNNGTG 1 cut(s) 667
Eam1104I CTCTTC 2 cut(s) 72, 630
EarI CTCTTC 2 cut(s) 72, 630
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 578
Eco31I GGTCTC 1 cut(s) 1032
Eco47I GGWCC 3 cut(s) 350, 477, 608
Eco88I CYCGRG 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 578
EcoT22I ATGCAT 1 cut(s) 865
ErhI CCWWGG 1 cut(s) 578
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 6 cut(s) 461, 582, 608, 668, 712, 800
FaqI GGGAC 1 cut(s) 990
FatI CATG 6 cut(s) 457, 578, 604, 664, 708, 796
FbaI TGATCA 2 cut(s) 189, 922
Fnu4HI GCNGC 5 cut(s) 136, 384, 393, 524, 1164
FokI GGATG 2 cut(s) 532, 959
Fsp4HI GCNGC 5 cut(s) 136, 384, 393, 524, 1164
FspBI CTAG 4 cut(s) 432, 789, 980, 1160
GlaI GCGC 2 cut(s) 633, 818
GluI GCNGC 5 cut(s) 136, 384, 393, 524, 1164
GsaI CCCAGC 1 cut(s) 214
GsuI CTGGAG 1 cut(s) 909
HaeII RGCGCY 1 cut(s) 635
HapII CCGG 4 cut(s) 185, 353, 379, 1043
HgaI GACGC 1 cut(s) 238
HhaI GCGC 2 cut(s) 634, 819
Hin1I GRCGYC 1 cut(s) 230
Hin1II CATG 6 cut(s) 461, 582, 608, 668, 712, 800
Hin6I GCGC 2 cut(s) 632, 817
HinP1I GCGC 2 cut(s) 632, 817
HinfI GANTC 8 cut(s) 45, 117, 202, 266, 425, 574, 720, 1064
HpaII CCGG 4 cut(s) 185, 353, 379, 1043
HphI GGTGA 3 cut(s) 34, 62, 199
Hpy166II GTNNAC 4 cut(s) 25, 350, 661, 670
Hpy188I TCNGA 4 cut(s) 207, 487, 695, 1093
Hpy188III TCNNGA 8 cut(s) 270, 458, 542, 548, 550, 871, 880, 1061
Hpy8I GTNNAC 4 cut(s) 25, 350, 661, 670
HpyAV CCTTC 2 cut(s) 10, 97
HpyCH4III ACNGT 2 cut(s) 74, 1075
HpyCH4V TGCA 4 cut(s) 523, 863, 999, 1099
HpyF10VI GCNNNNNNNGC 7 cut(s) 380, 389, 392, 568, 650, 809, 1163
HpyF3I CTNAG 5 cut(s) 11, 206, 692, 1092, 1167
Hsp92I GRCGYC 1 cut(s) 230
Hsp92II CATG 6 cut(s) 461, 582, 608, 668, 712, 800
HspAI GCGC 2 cut(s) 632, 817
Ksp22I TGATCA 2 cut(s) 189, 922
Kzo9I GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 1 cut(s) 890
Lsp1109I GCAGC 4 cut(s) 379, 395, 535, 1150
LweI GCATC 5 cut(s) 510, 549, 588, 850, 885
MaeI CTAG 4 cut(s) 432, 789, 980, 1160
MaeIII GTNAC 2 cut(s) 792, 1015
MalI GATC 6 cut(s) 191, 453, 546, 853, 924, 1049
MbiI CCGCTC 1 cut(s) 138
MboI GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
MboII GAAGA 6 cut(s) 59, 238, 332, 420, 446, 617
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 2 cut(s) 851, 1047
MhlI GDGCHC 3 cut(s) 469, 762, 895
MluCI AATT 6 cut(s) 55, 254, 492, 846, 994, 1138
MlyI GAGTC 3 cut(s) 54, 126, 1058
Mph1103I ATGCAT 1 cut(s) 865
MroXI GAANNNNTTC 1 cut(s) 624
MseI TTAA 4 cut(s) 437, 564, 780, 1137
MslI CAYNNNNRTG 2 cut(s) 342, 881
MspA1I CMGCKG 1 cut(s) 857
MspI CCGG 4 cut(s) 185, 353, 379, 1043
MspR9I CCNGG 2 cut(s) 380, 1043
MunI CAATTG 1 cut(s) 846
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 7 cut(s) 380, 389, 392, 568, 650, 809, 1163
NciI CCSGG 2 cut(s) 380, 1043
NcoI CCATGG 1 cut(s) 578
NdeII GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
NlaIII CATG 6 cut(s) 461, 582, 608, 668, 712, 800
NlaIV GGNNCC 2 cut(s) 759, 853
NmuCI GTSAC 1 cut(s) 792
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 1 cut(s) 800
PaeR7I CTCGAG 1 cut(s) 548
PagI TCATGA 1 cut(s) 457
PciI ACATGT 1 cut(s) 796
PciSI GCTCTTC 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 624
PfeI GAWTC 5 cut(s) 202, 266, 425, 574, 720
PinAI ACCGGT 1 cut(s) 352
PkrI GCNGC 5 cut(s) 137, 385, 394, 525, 1165
PleI GAGTC 3 cut(s) 53, 125, 1058
PpsI GAGTC 3 cut(s) 53, 125, 1058
PscI ACATGT 1 cut(s) 796
PspFI CCCAGC 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 759, 853
PspPI GGNCC 3 cut(s) 350, 477, 608
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 2 cut(s) 851, 1047
RsaI GTAC 1 cut(s) 660
RsaNI GTAC 1 cut(s) 659
RseI CAYNNNNRTG 2 cut(s) 342, 881
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 4 cut(s) 437, 564, 780, 1137
SatI GCNGC 5 cut(s) 136, 384, 393, 524, 1164
Sau3AI GATC 6 cut(s) 189, 451, 544, 851, 922, 1047
Sau96I GGNCC 3 cut(s) 350, 477, 608
SchI GAGTC 3 cut(s) 54, 126, 1058
ScrFI CCNGG 2 cut(s) 380, 1043
SduI GDGCHC 3 cut(s) 469, 762, 895
SfaNI GCATC 5 cut(s) 510, 549, 588, 850, 885
SfcI CTRYAG 1 cut(s) 654
Sfr274I CTCGAG 1 cut(s) 548
SinI GGWCC 3 cut(s) 350, 477, 608
SlaI CTCGAG 1 cut(s) 548
SmiMI CAYNNNNRTG 2 cut(s) 342, 881
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 6 cut(s) 55, 254, 492, 846, 994, 1138
SsiI CCGC 5 cut(s) 133, 136, 148, 843, 855
SspMI CTAG 4 cut(s) 432, 789, 980, 1160
StyD4I CCNGG 2 cut(s) 378, 1041
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 2 cut(s) 74, 1075
TaqI TCGA 4 cut(s) 549, 731, 774, 879
TasI AATT 6 cut(s) 55, 254, 492, 846, 994, 1138
TatI WGTACW 1 cut(s) 658
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 5 cut(s) 202, 266, 425, 574, 720
Tru1I TTAA 4 cut(s) 437, 564, 780, 1137
Tru9I TTAA 4 cut(s) 437, 564, 780, 1137
TscAI CASTG 1 cut(s) 925
TseFI GTSAC 1 cut(s) 792
TseI GCWGC 4 cut(s) 383, 392, 523, 1163
Tsp45I GTSAC 1 cut(s) 792
TspDTI ATGAA 4 cut(s) 330, 446, 474, 1166
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 1 cut(s) 925
VpaK11BI GGWCC 3 cut(s) 350, 477, 608
XapI RAATTY 1 cut(s) 55
XceI RCATGY 1 cut(s) 800
XhoI CTCGAG 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 624
XspI CTAG 4 cut(s) 432, 789, 980, 1160
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.