FvH4_3g19730

tetraacyldisaccharide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003555
Physical Location & Seq
Forward (+)
12905818 .. 12909262
3445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19730.t5

Sequence Viewer

Length: 1170 bp
ATGGAGAAACTGAGAAAAGCCGTGAATGAAATAGCGTATACTCAACACCACGCTAAGCTCTCTCATCTCCAACTCTCTCTAATCCCCATTCTCTTCTTCGCCTCCTCCCTCTACTCCGCCGCTCTCTCTCTCCGCCGCGCTCTCTACCGTTCCGGCCTCATAACAGAGCACCGGTTGCCGGTGCCGGTGATCAGTGTTGGGAATTTGACATGGGGAGGCAATGGGAAGACTCCAATGGTGGAGTTCATTGCGAATTACTTAGCTGATTCTGGGATTTCGCCTCTCATTCTTACAAGGGGTTATGCTGGTGGGGATGAGGCCAAGATGCTTTATAGGCATTTTCTTGGAAGGCCTGTGAAGATTGGTGTGGGTGCAAAACGAGCAGCTGTTGCTGCTCATTTTTTCGAAAGATATGGTTATGTTGATCCTTGCTCAAGTAAACACTCTGAGGGACTCTGCCATGGTGAGAAAGTGGGGAGTTGTCTTAGCTCAGAAAAGATTGGCACCGTAATTCTTGATGATGGAATGCAGCACTGGAGCTTGCAGCGAAATCTGGAGATTGTTATGATTAATGCATTAACATTATGGGGCAACTGTCACTTAATCCCACGTGGACCATTGAGGGAACCTCTCAATGCGCTTAGACGGGCAGATGTTGTTGTACTCCATCATGCAGATTTGGTTTCAAAACAGAATCTTGAAGAAACTGACTTAATGCTAAGAGAAGTTAACAAGTCTGTTCCTGTTTTCTTCACGAAAATGGATCCCTGTTATTTCTTCAACGTGGGAAATGCCACCTCCAGAAAACCTGTAGAAGATTTGTCTAACACTATTGTATTATGTGTTTCTGCCATTGGTTCTGCAAATGCATTTGCAAAGGGAATAGAGAAGATTGGAGCTTTTTATGTTGATCGACTTGAATTCAGTGACCACCACAAATTTCTGGCAAAGGATATTGAGATGATCAAAAGGAGGCTACGAGAACTGGAGGACAGGTTTAGTTCCAAGCCGGTTGTTATAGTAACAGAAAAGGATTATGATCGAGACCCAAATATTTTCAAGCACTTGGATCCTTATGAAGTTCTGTCACTCTGTTCCAAATTGAAGATCATACCTAACAGAGGATGCACTGAAGATAGCTTCAAAAAGATGTTGGAGGTTTGCAGGTGA

Protein Analysis

390

Amino Acids

43.71

Weight (kDa)

8.92

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LpxK PF02606 2 - 182 8.7e-25 Tetraacyldisaccharide-1-P 4'-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1155
Acc36I ACCTGC 1 cut(s) 1155
AccB1I GGYRCC 2 cut(s) 181, 503
AccBSI CCGCTC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 38
AccII CGCG 1 cut(s) 138
AciI CCGC 4 cut(s) 117, 120, 133, 136
AclWI GGATC 5 cut(s) 419, 758, 771, 1064, 1077
AcsI RAATTY 3 cut(s) 202, 920, 938
AcuI CTGAAG 1 cut(s) 1152
AcvI CACGTG 1 cut(s) 611
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 2 cut(s) 178, 1121
AgeI ACCGGT 1 cut(s) 171
AgsI TTSAA 7 cut(s) 687, 701, 781, 920, 1060, 1105, 1144
AluBI AGCT 7 cut(s) 58, 263, 386, 489, 540, 899, 1140
AluI AGCT 7 cut(s) 58, 263, 386, 489, 540, 899, 1140
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 1038
AlwI GGATC 5 cut(s) 419, 758, 771, 1064, 1077
AoxI GGCC 3 cut(s) 154, 318, 350
ApeKI GCWGC 4 cut(s) 383, 392, 529, 544
ApoI RAATTY 3 cut(s) 202, 920, 938
AseI ATTAAT 1 cut(s) 570
AsiGI ACCGGT 1 cut(s) 171
AspLEI GCGC 2 cut(s) 140, 640
AspS9I GGNCC 1 cut(s) 614
AsuHPI GGTGA 2 cut(s) 199, 476
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 1 cut(s) 614
BamHI GGATCC 2 cut(s) 763, 1069
BanI GGYRCC 2 cut(s) 181, 503
BbrPI CACGTG 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 171
BbvI GCAGC 4 cut(s) 379, 395, 541, 556
BccI CCATC 2 cut(s) 515, 675
BceAI ACGGC 1 cut(s) 5
BclI TGATCA 2 cut(s) 189, 963
BcoDI GTCTC 1 cut(s) 1038
BfmI CTRYAG 1 cut(s) 810
BfuAI ACCTGC 1 cut(s) 1155
BisI GCNGC 6 cut(s) 120, 136, 384, 393, 530, 545
BlpI GCTNAGC 1 cut(s) 54
BlsI GCNGC 6 cut(s) 121, 137, 385, 394, 531, 546
Bme18I GGWCC 1 cut(s) 614
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 5 cut(s) 183, 505, 627, 765, 1071
BmsI GCATC 2 cut(s) 315, 1115
BpiI GAAGAC 1 cut(s) 233
BplI GAGNNNNNCTC 2 cut(s) 613, 645
BpmI CTGGAG 4 cut(s) 556, 575, 784, 1007
Bpu1102I GCTNAGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 418
BsaAI YACGTR 1 cut(s) 611
BsaBI GATNNNNATC 1 cut(s) 1038
BsaI GGTCTC 1 cut(s) 1038
BsaJI CCNNGG 1 cut(s) 460
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 1121
Bse118I RCCGGY 4 cut(s) 171, 178, 184, 1009
Bse1I ACTGG 2 cut(s) 539, 990
Bse3DI GCAATG 2 cut(s) 226, 246
Bse8I GATNNNNATC 1 cut(s) 1038
BseDI CCNNGG 1 cut(s) 460
BseGI GGATG 2 cut(s) 319, 1130
BseJI GATNNNNATC 1 cut(s) 1038
BseLI CCNNNNNNNGG 2 cut(s) 178, 1121
BseMI GCAATG 2 cut(s) 226, 246
BseMII CTCAG 2 cut(s) 438, 504
BseNI ACTGG 2 cut(s) 539, 990
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 4 cut(s) 379, 395, 541, 556
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 3 cut(s) 156, 320, 352
BshNI GGYRCC 2 cut(s) 181, 503
BshTI ACCGGT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 5 cut(s) 153, 172, 179, 185, 1010
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 2 cut(s) 178, 1121
BsmAI GTCTC 1 cut(s) 1038
BsmFI GGGAC 1 cut(s) 465
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 3 cut(s) 156, 320, 352
Bso31I GGTCTC 1 cut(s) 1038
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
Bsp1720I GCTNAGC 1 cut(s) 54
Bsp19I CCATGG 1 cut(s) 460
BspACI CCGC 4 cut(s) 117, 120, 133, 136
BspANI GGCC 3 cut(s) 156, 320, 352
BspCNI CTCAG 2 cut(s) 439, 503
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 5 cut(s) 183, 505, 627, 765, 1071
BspMI ACCTGC 1 cut(s) 1155
BspPI GGATC 5 cut(s) 419, 758, 771, 1064, 1077
BspT104I TTCGAA 1 cut(s) 405
BspT107I GGYRCC 2 cut(s) 181, 503
BspTNI GGTCTC 1 cut(s) 1038
BsrBI CCGCTC 1 cut(s) 122
BsrDI GCAATG 2 cut(s) 226, 246
BsrFI RCCGGY 4 cut(s) 171, 178, 184, 1009
BsrI ACTGG 2 cut(s) 539, 990
BssAI RCCGGY 4 cut(s) 171, 178, 184, 1009
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
BssNAI GTATAC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 460
Bst1107I GTATAC 1 cut(s) 39
Bst4CI ACNGT 3 cut(s) 149, 508, 596
Bst6I CTCTTC 1 cut(s) 98
BstAPI GCANNNNNTGC 2 cut(s) 175, 389
BstBAI YACGTR 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 542
BstDEI CTNAG 8 cut(s) 11, 54, 259, 447, 485, 490, 641, 719
BstDSI CCRYGG 1 cut(s) 460
BstENI CCTNNNNNAGG 1 cut(s) 1119
BstF5I GGATG 2 cut(s) 319, 1130
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 2 cut(s) 140, 640
BstKTI GATC 8 cut(s) 192, 427, 766, 913, 966, 1042, 1072, 1110
BstMAI GTCTC 1 cut(s) 1038
BstMBI GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
BstMWI GCNNNNNNNGC 5 cut(s) 175, 334, 380, 389, 392
BstSFI CTRYAG 1 cut(s) 810
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 4 cut(s) 379, 395, 541, 556
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 2 cut(s) 763, 1069
BstYI RGATCY 2 cut(s) 763, 1069
BstZ17I GTATAC 1 cut(s) 39
BsuRI GGCC 3 cut(s) 156, 320, 352
BtgI CCRYGG 1 cut(s) 460
BtsCI GGATG 2 cut(s) 319, 1130
BtsIMutI CAGTG 4 cut(s) 199, 532, 931, 1128
BveI ACCTGC 1 cut(s) 1155
Cac8I GCNNGC 1 cut(s) 542
CfoI GCGC 2 cut(s) 140, 640
Cfr10I RCCGGY 4 cut(s) 171, 178, 184, 1009
Cfr13I GGNCC 1 cut(s) 614
Csp6I GTAC 1 cut(s) 662
CspAI ACCGGT 1 cut(s) 171
CviAII CATG 3 cut(s) 210, 461, 671
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 8 cut(s) 11, 54, 259, 447, 485, 490, 641, 719
DpnI GATC 8 cut(s) 191, 426, 765, 912, 965, 1041, 1071, 1109
DpnII GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
EciI GGCGGA 2 cut(s) 106, 122
Eco130I CCWWGG 1 cut(s) 460
Eco147I AGGCCT 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 1038
Eco47I GGWCC 1 cut(s) 614
Eco57I CTGAAG 1 cut(s) 1152
Eco72I CACGTG 1 cut(s) 611
EcoNI CCTNNNNNAGG 1 cut(s) 1119
EcoRI GAATTC 1 cut(s) 920
EcoT14I CCWWGG 1 cut(s) 460
EcoT22I ATGCAT 2 cut(s) 577, 871
ErhI CCWWGG 1 cut(s) 460
FaeI CATG 3 cut(s) 213, 464, 674
FaqI GGGAC 1 cut(s) 465
FatI CATG 3 cut(s) 209, 460, 670
FbaI TGATCA 2 cut(s) 189, 963
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 6 cut(s) 120, 136, 384, 393, 530, 545
FokI GGATG 2 cut(s) 326, 1137
Fsp4HI GCNGC 6 cut(s) 120, 136, 384, 393, 530, 545
GlaI GCGC 2 cut(s) 139, 639
GluI GCNGC 6 cut(s) 120, 136, 384, 393, 530, 545
GsuI CTGGAG 4 cut(s) 556, 575, 784, 1007
HaeIII GGCC 3 cut(s) 156, 320, 352
HapII CCGG 5 cut(s) 153, 172, 179, 185, 1010
HhaI GCGC 2 cut(s) 140, 640
Hin1II CATG 3 cut(s) 213, 464, 674
Hin6I GCGC 2 cut(s) 138, 638
HinP1I GCGC 2 cut(s) 138, 638
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HinfI GANTC 4 cut(s) 229, 266, 453, 694
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 5 cut(s) 153, 172, 179, 185, 1010
HphI GGTGA 2 cut(s) 199, 476
Hpy166II GTNNAC 4 cut(s) 39, 440, 614, 730
Hpy188I TCNGA 2 cut(s) 448, 493
Hpy188III TCNNGA 6 cut(s) 515, 554, 698, 754, 801, 1043
Hpy8I GTNNAC 4 cut(s) 39, 440, 614, 730
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 3 cut(s) 149, 508, 596
HpyCH4IV ACGT 2 cut(s) 610, 783
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 334, 380, 389, 392
HpyF3I CTNAG 8 cut(s) 11, 54, 259, 447, 485, 490, 641, 719
HpySE526I ACGT 2 cut(s) 610, 783
Hsp92II CATG 3 cut(s) 213, 464, 674
HspAI GCGC 2 cut(s) 138, 638
Ksp22I TGATCA 2 cut(s) 189, 963
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
LmnI GCTCC 2 cut(s) 537, 896
Lsp1109I GCAGC 4 cut(s) 379, 395, 541, 556
LweI GCATC 2 cut(s) 315, 1115
MaeII ACGT 2 cut(s) 610, 783
MaeIII GTNAC 4 cut(s) 596, 926, 1021, 1086
MalI GATC 8 cut(s) 191, 426, 765, 912, 965, 1041, 1071, 1109
MbiI CCGCTC 1 cut(s) 122
MboI GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
MflI RGATCY 2 cut(s) 763, 1069
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 6 cut(s) 202, 253, 510, 920, 938, 1100
MlyI GAGTC 2 cut(s) 223, 447
MmeI TCCRAC 2 cut(s) 94, 1134
Mph1103I ATGCAT 2 cut(s) 577, 871
MseI TTAA 5 cut(s) 570, 578, 602, 713, 729
MslI CAYNNNNRTG 1 cut(s) 758
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 5 cut(s) 153, 172, 179, 185, 1010
Mva1269I GAATGC 1 cut(s) 531
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 5 cut(s) 175, 334, 380, 389, 392
NcoI CCATGG 1 cut(s) 460
NdeII GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
NlaIII CATG 3 cut(s) 213, 464, 674
NlaIV GGNNCC 5 cut(s) 183, 505, 627, 765, 1071
NmuCI GTSAC 3 cut(s) 596, 926, 1086
NsiI ATGCAT 2 cut(s) 577, 871
NspV TTCGAA 1 cut(s) 405
PaqCI CACCTGC 1 cut(s) 1155
PceI AGGCCT 1 cut(s) 352
PctI GAATGC 1 cut(s) 531
PfeI GAWTC 2 cut(s) 266, 694
PinAI ACCGGT 1 cut(s) 171
PkrI GCNGC 6 cut(s) 121, 137, 385, 394, 531, 546
PleI GAGTC 2 cut(s) 223, 447
PmaCI CACGTG 1 cut(s) 611
PmlI CACGTG 1 cut(s) 611
PpsI GAGTC 2 cut(s) 223, 447
Ppu21I YACGTR 1 cut(s) 611
PshBI ATTAAT 1 cut(s) 570
PspCI CACGTG 1 cut(s) 611
PspN4I GGNNCC 5 cut(s) 183, 505, 627, 765, 1071
PspPI GGNCC 1 cut(s) 614
PsuI RGATCY 2 cut(s) 763, 1069
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 758
SaqAI TTAA 5 cut(s) 570, 578, 602, 713, 729
SatI GCNGC 6 cut(s) 120, 136, 384, 393, 530, 545
Sau3AI GATC 8 cut(s) 189, 424, 763, 910, 963, 1039, 1069, 1107
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 2 cut(s) 223, 447
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 2 cut(s) 315, 1115
SfcI CTRYAG 1 cut(s) 810
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 758
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 6 cut(s) 202, 253, 510, 920, 938, 1100
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 4 cut(s) 117, 120, 133, 136
SspI AATATT 1 cut(s) 1054
StuI AGGCCT 1 cut(s) 352
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 3 cut(s) 149, 508, 596
TaiI ACGT 2 cut(s) 613, 786
TaqI TCGA 3 cut(s) 405, 913, 1042
TasI AATT 6 cut(s) 202, 253, 510, 920, 938, 1100
TatI WGTACW 1 cut(s) 661
TauI GCSGC 2 cut(s) 122, 138
TfiI GAWTC 2 cut(s) 266, 694
Tru1I TTAA 5 cut(s) 570, 578, 602, 713, 729
Tru9I TTAA 5 cut(s) 570, 578, 602, 713, 729
TscAI CASTG 4 cut(s) 199, 539, 931, 1135
TseFI GTSAC 3 cut(s) 596, 926, 1086
TseI GCWGC 4 cut(s) 383, 392, 529, 544
Tsp45I GTSAC 3 cut(s) 596, 926, 1086
TspDTI ATGAA 3 cut(s) 42, 235, 1092
TspRI CASTG 4 cut(s) 199, 539, 931, 1135
VpaK11BI GGWCC 1 cut(s) 614
VspI ATTAAT 1 cut(s) 570
XagI CCTNNNNNAGG 1 cut(s) 1119
XapI RAATTY 3 cut(s) 202, 920, 938
XmiI GTMKAC 1 cut(s) 38
Zsp2I ATGCAT 2 cut(s) 577, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.