Rorug05G0136000

tetraacyldisaccharide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003555
Physical Location & Seq
Reverse (-)
12263106 .. 12265593
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0136000.1

Sequence Viewer

Length: 570 bp
ATGGGGTCGCTCGAAGAAGGCTTGGAGACCCTCAATTATCTGGACAACCTGCAGAACAGGAAATGTTGCACTGAGCAAGGGGTTGCTATAACATTTGAATCGGAGATGGACAAGATATATCTTAGCACTCCTACAAAGATTGCAATTATGGATCATGAGAAGAAGAGAACATTTGTAATACGCAAAGATGGTCTTCCAGATGCCGTGGTGTGGAATCCTTGGGATAAGAAGGCAAAGGCTATGGCTGATTTGGAGATGATGAAGGCTGCTGATATTGAGAAACCTATCACATTGAAAACTGAAGAGGAGTGGAGAGGGGGGCTGGAGCAAGAATACAAGTATCTGCAAAGGTTCAGAGTAGCAATCACTCTTGGTGCTACAATTCTACATCTGGAGTCTGATAATCATGTGATGACTGTAACTTGTGCTTTTGTAAAGGGAATCCAAAGGCTTCCTAGGCCCAAGATAAACCCTTTCGGCGCATGTGAAATGCTCCAACTGTGCATTGCAGCACAGTGCCTGGCGCATTTTATAACCTTTGTTGAATCAGGCATATCTCAGTGCAATTAA

Protein Analysis

189

Amino Acids

21.47

Weight (kDa)

5.86

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldose_epim PF01263 7 - 106 1.4e-10 Aldose 1-epimerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
Acc36I ACCTGC 1 cut(s) 57
AclWI GGATC 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 210
AgsI TTSAA 3 cut(s) 98, 295, 545
AjnI CCWGG 1 cut(s) 519
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 1 cut(s) 159
AlwNI CAGNNNCTG 1 cut(s) 520
AoxI GGCC 1 cut(s) 458
ApeKI GCWGC 2 cut(s) 266, 509
AspA2I CCTAGG 1 cut(s) 455
AspLEI GCGC 2 cut(s) 482, 526
AspS9I GGNCC 1 cut(s) 459
AvrII CCTAGG 1 cut(s) 455
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 2 cut(s) 253, 521
BccI CCATC 2 cut(s) 100, 182
BceAI ACGGC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 521
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 57
BisI GCNGC 2 cut(s) 267, 510
BlnI CCTAGG 1 cut(s) 455
BlsI GCNGC 2 cut(s) 268, 511
Bme1390I CCNGG 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 459
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 2 cut(s) 344, 413
BsaI GGTCTC 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 204, 218, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 504
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 3 cut(s) 204, 218, 455
BseLI CCNNNNNNNGG 1 cut(s) 210
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 1 cut(s) 63
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 253, 521
BshFI GGCC 1 cut(s) 460
BslI CCNNNNNNNGG 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 20
BsnI GGCC 1 cut(s) 460
Bso31I GGTCTC 1 cut(s) 20
Bsp143I GATC 1 cut(s) 151
BspANI GGCC 1 cut(s) 460
BspCNI CTCAG 1 cut(s) 64
BspHI TCATGA 1 cut(s) 154
BspMAI CTGCAG 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 57
BspPI GGATC 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 504
BssECI CCNNGG 3 cut(s) 204, 218, 455
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 2 cut(s) 218, 455
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 3 cut(s) 418, 501, 516
Bst6I CTCTTC 2 cut(s) 158, 297
BstDEI CTNAG 3 cut(s) 72, 122, 558
BstDSI CCRYGG 1 cut(s) 204
BstHHI GCGC 2 cut(s) 482, 526
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 1 cut(s) 457
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 1 cut(s) 486
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 253, 521
BstV2I GAAGAC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 204
BtsIMutI CAGTG 3 cut(s) 69, 521, 566
BveI ACCTGC 1 cut(s) 57
CaiI CAGNNNCTG 1 cut(s) 520
CciI TCATGA 1 cut(s) 154
CfoI GCGC 2 cut(s) 482, 526
Cfr13I GGNCC 1 cut(s) 459
CviAII CATG 3 cut(s) 155, 407, 483
CviJI RGCY 7 cut(s) 21, 239, 245, 266, 322, 451, 460
CviKI_1 RGCY 7 cut(s) 21, 239, 245, 266, 322, 451, 460
DdeI CTNAG 3 cut(s) 72, 122, 558
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 158, 297
EarI CTCTTC 2 cut(s) 158, 297
Eco130I CCWWGG 2 cut(s) 218, 455
Eco31I GGTCTC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 2 cut(s) 218, 455
ErhI CCWWGG 2 cut(s) 218, 455
FaeI CATG 3 cut(s) 158, 410, 486
FaiI YATR 9 cut(s) 89, 118, 149, 156, 242, 408, 484, 533, 554
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FatI CATG 3 cut(s) 154, 406, 482
Fnu4HI GCNGC 2 cut(s) 267, 510
Fsp4HI GCNGC 2 cut(s) 267, 510
FspBI CTAG 1 cut(s) 456
GlaI GCGC 2 cut(s) 481, 525
GluI GCNGC 2 cut(s) 267, 510
GsuI CTGGAG 2 cut(s) 344, 413
HaeIII GGCC 1 cut(s) 460
HhaI GCGC 2 cut(s) 482, 526
Hin1II CATG 3 cut(s) 158, 410, 486
Hin6I GCGC 2 cut(s) 480, 524
HinP1I GCGC 2 cut(s) 480, 524
HinfI GANTC 5 cut(s) 98, 214, 395, 441, 545
Hpy188I TCNGA 3 cut(s) 103, 356, 400
Hpy188III TCNNGA 4 cut(s) 41, 155, 197, 392
HpyAV CCTTC 3 cut(s) 11, 223, 256
HpyCH4III ACNGT 3 cut(s) 418, 501, 516
HpyCH4V TGCA 7 cut(s) 52, 69, 143, 346, 504, 509, 564
HpyF10VI GCNNNNNNNGC 1 cut(s) 457
HpyF3I CTNAG 3 cut(s) 72, 122, 558
Hsp92II CATG 3 cut(s) 158, 410, 486
HspAI GCGC 2 cut(s) 480, 524
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 2 cut(s) 325, 498
LpnPI CCDG 9 cut(s) 26, 43, 62, 210, 308, 377, 506, 533, 534
Lsp1109I GCAGC 2 cut(s) 253, 521
LweI GCATC 1 cut(s) 190
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 418
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 5 cut(s) 26, 172, 175, 185, 314
MluCI AATT 4 cut(s) 34, 144, 381, 565
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 3 cut(s) 41, 298, 308
MseI TTAA 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 1 cut(s) 457
NdeII GATC 1 cut(s) 151
NlaIII CATG 3 cut(s) 158, 410, 486
NspI RCATGY 1 cut(s) 486
PagI TCATGA 1 cut(s) 154
PfeI GAWTC 4 cut(s) 98, 214, 441, 545
PkrI GCNGC 2 cut(s) 268, 511
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
PsiI TTATAA 1 cut(s) 533
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspPI GGNCC 1 cut(s) 459
PstI CTGCAG 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 520
SaqAI TTAA 1 cut(s) 568
SatI GCNGC 2 cut(s) 267, 510
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 459
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 521
SetI ASST 4 cut(s) 51, 286, 353, 539
SfaNI GCATC 1 cut(s) 190
SfcI CTRYAG 1 cut(s) 50
Sse9I AATT 4 cut(s) 34, 144, 381, 565
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 2 cut(s) 218, 455
TaaI ACNGT 3 cut(s) 418, 501, 516
TaqI TCGA 1 cut(s) 12
TasI AATT 4 cut(s) 34, 144, 381, 565
TfiI GAWTC 4 cut(s) 98, 214, 441, 545
Tru1I TTAA 1 cut(s) 568
Tru9I TTAA 1 cut(s) 568
TscAI CASTG 3 cut(s) 76, 521, 566
TseI GCWGC 2 cut(s) 266, 509
TspDTI ATGAA 1 cut(s) 275
TspRI CASTG 3 cut(s) 76, 521, 566
XceI RCATGY 1 cut(s) 486
XmaJI CCTAGG 1 cut(s) 455
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.