Rh5AG228300

tetraacyldisaccharide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003555
Physical Location & Seq
Forward (+)
27444039 .. 27446771
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG228300.1

Sequence Viewer

Length: 1161 bp
ATGGAGAAACTGAGAAAAGCCGTGAATGAAATGGCGTATACTCAACACCACGCTAAGCTCTCTCATCTTCAACTCTCTCTAATCCCCGTCCTCTTCTTGGCCTCATCTCTCTACAAGGCCGCTCTCTCTCTCCGCCGCGCTCTCTACCATTCCGGCTTCTTAAGACAGCACCGGTTGCCGGTGCCGGTGATCAGTGTTGGCAATTTGACATGGGGAGGCAATGGGAAAACTCCAATGGTGGAGTTCATTGCGAACTATTTAGCTGATTCAGGGATTTCACCTCTCATTCTGACCAGGGGTTATGCTGGTGGGGATGAGGCCAAGATGCTTCATAGGCATTTCCTTGGAAGGCCTGTTAAGATCGGTGTGGGTGCAAAGCGAGCAGCCGTTGCTGCTAGTTTTTTCGAAAGATATGGTTATGTTGAACCTAGCTCTAGTAAACTCTGCCATGGTCAGAAAGTGGGGAGTCATCTTAGTTCAGAAAAGATTGGCACTGTAATTCTTGATGATGGAATGCAGCACTGGAGCTTGCAGCGGAATCTGGAGATTGTTATGATTAATGGATTAACATTATGGGGCAACTGTCACTTAATCCCACGTGGACCGTTGAGGGAACCTCTCAGTGCACTTAGACAGGCAGATGTTGTTGTACTCCATCACGCAGATTTGGTTTCAGAACAAAATCTCGAAGATACTGACTTAATGCTAAGAGAAGTTAACAAGTCTGTTCCTGTTTTCTTCACAAAAATGGATCCTTGTTATTTCTTTGACGTGGGAAATGCCACCTCTAGAAAACATGTAGGAGATTTGTCTAACACTATTGTATTATGTGTTACTGCCATTGGTTCTGCAAATTCATTTGCAAAGGGAATAGAGAAGATTGGAGCATTTTATGTTGATAGACTTGAATTCAGTGACCACCACAAATTTCTGGCAAAGGATATTGAGATAATCAAAAGGAGGCTACGAGAACTGGAGGACAGGTTTAGTTCCAAGCCGGCTGTTATAGTAACAGAAAAGGATTATGATCGAGACCCATATATTTTCAAGCACTTGGATCCTTTTGAAGTTCTGGCACTCTGTTCTGAATTGAAGATCATACCTAACAGAGGATGCACTGAAGAAAGCTTCAAAAAATTGTTGAAGGAGGTTTGCACATGA

Protein Analysis

386

Amino Acids

43.29

Weight (kDa)

8.92

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LpxK PF02606 30 - 369 8.2e-65 Tetraacyldisaccharide-1-P 4'-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AccBSI CCGCTC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 38
AccII CGCG 1 cut(s) 138
AciI CCGC 4 cut(s) 120, 133, 136, 535
AclWI GGATC 4 cut(s) 746, 759, 1052, 1065
AcsI RAATTY 3 cut(s) 853, 908, 926
AcuI CTGAAG 1 cut(s) 1140
AcvI CACGTG 1 cut(s) 599
AfaI GTAC 1 cut(s) 651
AfiI CCNNNNNNNGG 3 cut(s) 97, 178, 1109
AflII CTTAAG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 796
AgeI ACCGGT 1 cut(s) 171
AgsI TTSAA 8 cut(s) 71, 425, 908, 1048, 1067, 1093, 1132, 1144
AjiI CACGTC 1 cut(s) 772
AjnI CCWGG 1 cut(s) 293
AluBI AGCT 5 cut(s) 58, 263, 432, 528, 1128
AluI AGCT 5 cut(s) 58, 263, 432, 528, 1128
Alw21I GWGCWC 1 cut(s) 628
Alw26I GTCTC 1 cut(s) 1026
Alw44I GTGCAC 1 cut(s) 624
AlwI GGATC 4 cut(s) 746, 759, 1052, 1065
AoxI GGCC 4 cut(s) 99, 117, 318, 350
ApaLI GTGCAC 1 cut(s) 624
ApeKI GCWGC 4 cut(s) 383, 392, 517, 532
ApoI RAATTY 3 cut(s) 853, 908, 926
AseI ATTAAT 1 cut(s) 558
AsiGI ACCGGT 1 cut(s) 171
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 1 cut(s) 602
AsuHPI GGTGA 2 cut(s) 199, 270
AsuII TTCGAA 1 cut(s) 405
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 628
BamHI GGATCC 2 cut(s) 751, 1057
BanI GGYRCC 1 cut(s) 181
BbrPI CACGTG 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 4 cut(s) 379, 395, 529, 544
BccI CCATC 2 cut(s) 503, 663
BceAI ACGGC 2 cut(s) 5, 371
BciT130I CCWGG 1 cut(s) 295
BclI TGATCA 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 1026
BfaI CTAG 4 cut(s) 396, 429, 435, 789
BfrI CTTAAG 1 cut(s) 160
BisI GCNGC 6 cut(s) 120, 136, 384, 393, 518, 533
BlpI GCTNAGC 1 cut(s) 54
BlsI GCNGC 6 cut(s) 121, 137, 385, 394, 519, 534
Bme1390I CCNGG 1 cut(s) 295
Bme18I GGWCC 1 cut(s) 602
BmgBI CACGTC 1 cut(s) 772
BmgT120I GGNCC 1 cut(s) 602
BmiI GGNNCC 4 cut(s) 183, 615, 753, 1059
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 2 cut(s) 315, 1103
BplI GAGNNNNNCTC 2 cut(s) 601, 633
BpmI CTGGAG 3 cut(s) 544, 563, 995
Bpu1102I GCTNAGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 599
BsaBI GATNNNNATC 1 cut(s) 1026
BsaI GGTCTC 1 cut(s) 1026
BsaJI CCNNGG 3 cut(s) 294, 343, 448
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 178, 1109
Bse118I RCCGGY 4 cut(s) 171, 178, 184, 997
Bse1I ACTGG 2 cut(s) 527, 978
Bse3DI GCAATG 2 cut(s) 226, 246
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 294, 343, 448
BseGI GGATG 2 cut(s) 319, 1118
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 3 cut(s) 97, 178, 1109
BseMI GCAATG 2 cut(s) 226, 246
BseMII CTCAG 1 cut(s) 634
BseNI ACTGG 2 cut(s) 527, 978
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 4 cut(s) 379, 395, 529, 544
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 4 cut(s) 101, 119, 320, 352
BshNI GGYRCC 1 cut(s) 181
BshTI ACCGGT 1 cut(s) 171
BsiHKAI GWGCWC 1 cut(s) 628
BsiSI CCGG 5 cut(s) 153, 172, 179, 185, 998
BslI CCNNNNNNNGG 3 cut(s) 97, 178, 1109
BsmAI GTCTC 1 cut(s) 1026
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 4 cut(s) 101, 119, 320, 352
Bso31I GGTCTC 1 cut(s) 1026
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
Bsp1720I GCTNAGC 1 cut(s) 54
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 4 cut(s) 120, 133, 136, 535
BspANI GGCC 4 cut(s) 101, 119, 320, 352
BspCNI CTCAG 1 cut(s) 633
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 4 cut(s) 183, 615, 753, 1059
BspPI GGATC 4 cut(s) 746, 759, 1052, 1065
BspT104I TTCGAA 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 181
BspTI CTTAAG 1 cut(s) 160
BspTNI GGTCTC 1 cut(s) 1026
BsrBI CCGCTC 1 cut(s) 122
BsrDI GCAATG 2 cut(s) 226, 246
BsrFI RCCGGY 4 cut(s) 171, 178, 184, 997
BsrI ACTGG 2 cut(s) 527, 978
BssAI RCCGGY 4 cut(s) 171, 178, 184, 997
BssECI CCNNGG 3 cut(s) 294, 343, 448
BssMI GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
BssNAI GTATAC 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 343, 448
Bst1107I GTATAC 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 295
Bst4CI ACNGT 3 cut(s) 496, 584, 606
Bst6I CTCTTC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 160
BstAPI GCANNNNNTGC 2 cut(s) 175, 389
BstBAI YACGTR 1 cut(s) 599
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 3 cut(s) 381, 530, 999
BstDEI CTNAG 6 cut(s) 11, 54, 473, 620, 629, 707
BstDSI CCRYGG 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 1107
BstF5I GGATG 2 cut(s) 319, 1118
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 6 cut(s) 192, 363, 754, 1030, 1060, 1098
BstMAI GTCTC 1 cut(s) 1026
BstMBI GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
BstMWI GCNNNNNNNGC 5 cut(s) 175, 334, 380, 389, 392
BstNI CCWGG 1 cut(s) 295
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 1 cut(s) 293
BstSLI GKGCMC 1 cut(s) 628
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 4 cut(s) 379, 395, 529, 544
BstX2I RGATCY 2 cut(s) 751, 1057
BstYI RGATCY 2 cut(s) 751, 1057
BstZ17I GTATAC 1 cut(s) 39
BsuRI GGCC 4 cut(s) 101, 119, 320, 352
BtgI CCRYGG 1 cut(s) 448
BtrI CACGTC 1 cut(s) 772
BtsCI GGATG 2 cut(s) 319, 1118
BtsIMutI CAGTG 6 cut(s) 199, 492, 520, 628, 919, 1116
Cac8I GCNNGC 3 cut(s) 381, 530, 999
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 4 cut(s) 171, 178, 184, 997
Cfr13I GGNCC 1 cut(s) 602
Csp6I GTAC 1 cut(s) 650
CspAI ACCGGT 1 cut(s) 171
CviAII CATG 4 cut(s) 210, 449, 797, 1158
CviQI GTAC 1 cut(s) 650
DdeI CTNAG 6 cut(s) 11, 54, 473, 620, 629, 707
DpnI GATC 6 cut(s) 191, 362, 753, 1029, 1059, 1097
DpnII GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 343, 448
Eco147I AGGCCT 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 1026
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 1140
Eco72I CACGTG 1 cut(s) 599
EcoNI CCTNNNNNAGG 1 cut(s) 1107
EcoRI GAATTC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 293
EcoT14I CCWWGG 2 cut(s) 343, 448
ErhI CCWWGG 2 cut(s) 343, 448
FaeI CATG 4 cut(s) 213, 452, 800, 1161
FatI CATG 4 cut(s) 209, 448, 796, 1157
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 6 cut(s) 120, 136, 384, 393, 518, 533
FokI GGATG 2 cut(s) 326, 1125
Fsp4HI GCNGC 6 cut(s) 120, 136, 384, 393, 518, 533
FspBI CTAG 4 cut(s) 396, 429, 435, 789
GlaI GCGC 1 cut(s) 139
GluI GCNGC 6 cut(s) 120, 136, 384, 393, 518, 533
GsuI CTGGAG 3 cut(s) 544, 563, 995
HaeIII GGCC 4 cut(s) 101, 119, 320, 352
HapII CCGG 5 cut(s) 153, 172, 179, 185, 998
HhaI GCGC 1 cut(s) 140
Hin1II CATG 4 cut(s) 213, 452, 800, 1161
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HincII GTYRAC 1 cut(s) 718
HindII GTYRAC 1 cut(s) 718
HindIII AAGCTT 1 cut(s) 1126
HinfI GANTC 3 cut(s) 266, 466, 538
HpaI GTTAAC 1 cut(s) 718
HpaII CCGG 5 cut(s) 153, 172, 179, 185, 998
HphI GGTGA 2 cut(s) 199, 270
Hpy166II GTNNAC 5 cut(s) 39, 440, 602, 626, 718
Hpy188I TCNGA 5 cut(s) 291, 456, 481, 676, 1087
Hpy188III TCNNGA 5 cut(s) 503, 542, 686, 789, 1031
Hpy8I GTNNAC 5 cut(s) 39, 440, 602, 626, 718
HpyAV CCTTC 2 cut(s) 342, 1138
HpyCH4III ACNGT 3 cut(s) 496, 584, 606
HpyCH4IV ACGT 2 cut(s) 598, 771
HpyCH4V TGCA 8 cut(s) 374, 517, 532, 626, 851, 863, 1116, 1155
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 334, 380, 389, 392
HpyF3I CTNAG 6 cut(s) 11, 54, 473, 620, 629, 707
HpySE526I ACGT 2 cut(s) 598, 771
Hsp92II CATG 4 cut(s) 213, 452, 800, 1161
HspAI GCGC 1 cut(s) 138
KroI GCCGGC 1 cut(s) 997
KroNI GCCGGC 1 cut(s) 999
Ksp22I TGATCA 1 cut(s) 189
KspAI GTTAAC 1 cut(s) 718
Kzo9I GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
LmnI GCTCC 2 cut(s) 525, 884
Lsp1109I GCAGC 4 cut(s) 379, 395, 529, 544
LweI GCATC 2 cut(s) 315, 1103
MaeI CTAG 4 cut(s) 396, 429, 435, 789
MaeII ACGT 2 cut(s) 598, 771
MaeIII GTNAC 4 cut(s) 584, 832, 914, 1009
MalI GATC 6 cut(s) 191, 362, 753, 1029, 1059, 1097
MbiI CCGCTC 1 cut(s) 122
MboI GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
MboII GAAGA 7 cut(s) 59, 85, 701, 730, 889, 1105, 1133
MflI RGATCY 2 cut(s) 751, 1057
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 7 cut(s) 202, 498, 853, 908, 926, 1088, 1136
MlyI GAGTC 1 cut(s) 475
MroNI GCCGGC 1 cut(s) 997
MseI TTAA 7 cut(s) 161, 357, 558, 566, 590, 701, 717
MslI CAYNNNNRTG 1 cut(s) 746
MspA1I CMGCKG 1 cut(s) 535
MspCI CTTAAG 1 cut(s) 160
MspI CCGG 5 cut(s) 153, 172, 179, 185, 998
MspR9I CCNGG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 519
MvaI CCWGG 1 cut(s) 295
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 5 cut(s) 175, 334, 380, 389, 392
NaeI GCCGGC 1 cut(s) 999
NcoI CCATGG 1 cut(s) 448
NdeII GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
NgoMIV GCCGGC 1 cut(s) 997
NlaIII CATG 4 cut(s) 213, 452, 800, 1161
NlaIV GGNNCC 4 cut(s) 183, 615, 753, 1059
NmuCI GTSAC 2 cut(s) 584, 914
NspI RCATGY 1 cut(s) 800
NspV TTCGAA 1 cut(s) 405
PceI AGGCCT 1 cut(s) 352
PciI ACATGT 1 cut(s) 796
PctI GAATGC 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 999
PfeI GAWTC 2 cut(s) 266, 538
PinAI ACCGGT 1 cut(s) 171
PkrI GCNGC 6 cut(s) 121, 137, 385, 394, 519, 534
PleI GAGTC 1 cut(s) 474
PmaCI CACGTG 1 cut(s) 599
PmlI CACGTG 1 cut(s) 599
PpsI GAGTC 1 cut(s) 474
Ppu21I YACGTR 1 cut(s) 599
PscI ACATGT 1 cut(s) 796
PshBI ATTAAT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 293
PspCI CACGTG 1 cut(s) 599
PspGI CCWGG 1 cut(s) 293
PspN4I GGNNCC 4 cut(s) 183, 615, 753, 1059
PspPI GGNCC 1 cut(s) 602
PsuI RGATCY 2 cut(s) 751, 1057
RsaI GTAC 1 cut(s) 651
RsaNI GTAC 1 cut(s) 650
RseI CAYNNNNRTG 1 cut(s) 746
SaqAI TTAA 7 cut(s) 161, 357, 558, 566, 590, 701, 717
SatI GCNGC 6 cut(s) 120, 136, 384, 393, 518, 533
Sau3AI GATC 6 cut(s) 189, 360, 751, 1027, 1057, 1095
Sau96I GGNCC 1 cut(s) 602
SchI GAGTC 1 cut(s) 475
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 2 cut(s) 315, 1103
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 7 cut(s) 202, 498, 853, 908, 926, 1088, 1136
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 4 cut(s) 120, 133, 136, 535
SspMI CTAG 4 cut(s) 396, 429, 435, 789
StuI AGGCCT 1 cut(s) 352
StyD4I CCNGG 1 cut(s) 293
StyI CCWWGG 2 cut(s) 343, 448
TaaI ACNGT 3 cut(s) 496, 584, 606
TaiI ACGT 2 cut(s) 601, 774
TaqI TCGA 3 cut(s) 405, 687, 1030
TasI AATT 7 cut(s) 202, 498, 853, 908, 926, 1088, 1136
TatI WGTACW 1 cut(s) 649
TauI GCSGC 2 cut(s) 122, 138
TfiI GAWTC 2 cut(s) 266, 538
Tru1I TTAA 7 cut(s) 161, 357, 558, 566, 590, 701, 717
Tru9I TTAA 7 cut(s) 161, 357, 558, 566, 590, 701, 717
TscAI CASTG 6 cut(s) 199, 499, 527, 628, 919, 1123
TseFI GTSAC 2 cut(s) 584, 914
TseI GCWGC 4 cut(s) 383, 392, 517, 532
Tsp45I GTSAC 2 cut(s) 584, 914
TspDTI ATGAA 4 cut(s) 42, 235, 320, 846
TspRI CASTG 6 cut(s) 199, 499, 527, 628, 919, 1123
Vha464I CTTAAG 1 cut(s) 160
VneI GTGCAC 1 cut(s) 624
VpaK11BI GGWCC 1 cut(s) 602
VspI ATTAAT 1 cut(s) 558
XagI CCTNNNNNAGG 1 cut(s) 1107
XapI RAATTY 3 cut(s) 853, 908, 926
XbaI TCTAGA 1 cut(s) 788
XceI RCATGY 1 cut(s) 800
XmiI GTMKAC 1 cut(s) 38
XspI CTAG 4 cut(s) 396, 429, 435, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.